Michel Pélandakis

Michel Pélandakis
Claude Bernard University Lyon 1 | UCBL · Unité de recherche en microbiologie, adaptation et pathogénie

PhD

About

65
Publications
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717
Citations

Publications

Publications (65)
Article
Poor bioavailability and low residence time limit the efficiency of conventional biguanide-based eye drops against Acanthamoeba keratitis. The aim of this work was to formulate an original anti-amoebic thermoreversible ocular gel combining biguanide and metalloproteases inhibitor - chelating agent. Chlorhexidine digluconate (CHX)-ethylenediaminetet...
Article
Full-text available
Free-living amoebae (FLA) are ubiquitous protozoa found worldwide in the environment. They feed by phagocytosis on various microorganisms. However, some bacteria, i.e., amoebae-resistant bacteria (ARB) or bacterial endocytobionts, can resist phagocytosis and even multiply inside FLA. This study investigated the diversity of culturable FLA in variou...
Article
Full-text available
An acute gastroenteritis (AG) outbreak occurred among participants in an obstacle race in France in the summer of 2015. An investigation in two phases was conducted to identify the source of infection and document the extent of the outbreak. First, a message on a social media website asked racers to report any symptoms by email to the Regional Heal...
Article
Full-text available
Naegleria sp. is a free living amoeba belonging to the Heterolobosea class. Over 40 species of Naegleria were identified and recovered worldwide in different habitats such as swimming pools, freshwater lakes, soil or dust. Among them, N. fowleri, is a human pathogen responsible for primary amoeboic meningoencephalitis (PAM). Around 300 cases were r...
Data
Alignment sequence of the 2 alleles (225bp and 252bp) of the NG42 locus. (DOCX)
Data
Assignment of the 47 N. fowleri strains with GENECLASS. (XLSX)
Data
N. fowleri analysis. N. fowleri strains examined before this study. (XLSX)
Article
Full-text available
Primary amoebic meningoencephalitis (PAM) is a fatal infection in most cases, caused by the amoeba flagellate Naegleria fowleri. This report describes the first cases of PAM in Algeria, in a cow and a ewe from Batna, north-eastern Algeria. The death of both ruminants occurred a week after the first clinical manifestations. The cerebrospinal fluid,...
Article
A mature male, black and white ruffed lemur (Varecia variegata variegata) died in a zoological garden after a 4-day history of lethargy and non-responsive convulsions. Necropsy and histopathological examinations revealed acute necrotizing and haemorrhagic meningoencephalitis with intralesional amoebas confirmed by immunohistochemistry. Acanthamoeba...
Article
Full-text available
We examined a partial SSU-rDNA sequence from 20 Acanthamoeba isolates associated with keratitis infections. The phylogenetic tree inferred from this partial sequence allowed to assign isolates to genotypes. Among the 20 isolates examined, 16 were found to be of the T4 genotype, 2 were T3, 1 was a T5, and 1 was a T2, confirming the predominance of T...
Article
Investigation of soil amoebae in 11 cooling towers allowed us to isolate a major unknown small-sized amoeba population (SZA). However, SZA did not appear to be specific to cooling tower ecosystems since they are also a major amoeba population found in muds isolated from different points of a water treatment plant. The SSU-rDNA sequences from SZA st...
Article
Its natural resistance to antiprotozoal chemotherapy characterizes the intestinal protozoan parasite Cryptosporidium parvum and the P-glycoprotein-related multidrug resistance proteins such as CpABC3 could be involved. In order to design and study specific inhibitors of the CpABC3 nucleotide-binding domain, a hexahistidine-tagged recombinant protei...
Article
Thirty-one Naegleria fowleri strains from different continents were studied by using the Random Amplified Polymorphic DNA (RAPD) method. Five primers among the 40 examined generated eight RAPD variants corresponding partially to their geographic origin. Four characteristic variants are detected in Oceania. The four remaining ones are present in Eur...
Article
Full-text available
A multiplex PCR was developed to simultaneously detect Naegleria fowleri and other Naegleria species in the environment. Multiplex PCR was also capable of identifying N. fowleri isolates with internal transcribed spacers of different sizes. In addition, restriction fragment length polymorphism analysis of the PCR product distinguished the main ther...
Article
The recovery efficiency of tangential flow microfiltration for protozoa has been evaluated using water artificially seeded with a free-living amoeba belonging to a non-pathogenic species of Naegleria: N. lovaniensis. After treatment, the recovery of trophozoites from the concentrate was 24±9.5%. This result was improved further by an elution step w...
Article
Internal transcribed spacers (ITS) and the 5.8S ribosomal gene of 21 Naegleria fowleri strains and eight other species including Naegleria gruberi were sequenced. The results showed that this region can help differentiate between and within species. The phylogeny of Naegleria spp. deduced from the ITS and the 5.8S gene produced four major lineages,...
Article
Full-text available
In this study, 30 strains of the pathogenic free-living amoeba Naegleria fowleri were investigated by using the randomly amplified polymorphic DNA (RAPD) method. The present study confirmed our previous finding that RAPD variation is not correlated with geographical origin. In particular, Mexican strains belong to the variant previously detected in...
Article
Full-text available
Detection of pathogenic Naegleria fowleri in environmental water samples, which is necessary for the prevention of primary amoebic meningoencephalitis, generally requires concentrating the samples. Two concentration techniques, filtration and centrifugation, were used to study the recovery of N. fowleri, in vegetative or cystic form, that had been...
Article
Thirty-one Naegleria fowleri strains from different continents were studied by using the Random Amplified Polymorphic DNA (RAPD) method. Five primers among the 40 examined generated eight RAPD variants corresponding partially to their geographic origin. Four characteristic variants are detected in Oceania. The four remaining ones are present in Eur...
Article
A yeast artificial chromosome (YAC) contig located in 6p23 and spanning roughly 2.5 Mb has been constructed from the content of 10 sequence tagged sites (STSs) for YAC clones in 66 yeast colonies. Nine of the STSs have been genetically mapped in CEPH families. The order of STSs mapped with the contig is consistent with that of the genetic map. The...
Article
Full-text available
Nucleotide sequences of 72 species of Drosophilidae were determined for divergent D1 and D2 domains (representing 200 and 341 nucleotides respectively in D. melanogaster) of large ribosomal RNA, using the rRNA direct sequencing method. Molecular phylogenetic trees were reconstructed using both distance and parsimony methods and the robustness of th...
Article
Full-text available
It has often been suggested that the frequently observed Watson-Crick base-pair compensatory substitutions in RNA helical structures occur mainly through a slightly deleterious G.U intermediate state. We have scored base substitutions in a set of 82 related Drosophila species for the D1 and D2 variable domains of the large rRNA subunit. In all loca...
Article
Full-text available
RNA sequencing has been used to assess the relationships among species of the subgenus Sophophora of the genus Drosophila. Two divergent domains, D1 and D2, of the large ribosomal RNA (28S), totalling 550 nucleotides have been sequenced using the rRNA direct sequencing method. A tree has been reconstructed from the neighbor-joining algorithm and th...
Book
For a long time, cytochrome c, which is a slowly evolving protein, was the reference molecule to establish molecular phylogenies for very divergent eukaryotic lineages (Fitch and Margoliash 1967). Considering bacterial and chloroplastic c-type cytochomes together with cytochrome c, the first universal phylogenetic tree has been established. However...
Article
The genomic probe CB1 recognizes sites of simple sequence homotogy between the human X and Y chromosomes. DNAs restricted with EcoRI from the great apes (Pan and Gorilla) were examined for sequences homologous to this probe, by molecular hybridization experiments. Results reported here revealed, by genetic dosage with sex and using an internal stan...
Article
Five cosmidic probes (7 b; 8 j; 22 b; CB 16 and 115 i 1) recognizes site of simple-copy DNA sequence homology between the human X and Y chromosomes. Genomic DNAS restricted with Eco RI from the great apes (Gorilla and Pan) were examined for sequences homologous to these probes, by molecular hybridization studies. Experiments reported here revealed,...

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