Michał Oskiera

Universität Bremen | Uni Bremen · Microbe-Plant Interactions

Microbiological analyses of lettuce, radish, carrot and beetroot were conducted to determine the effect of production system (organic and conventional) on the microbial quality of vegetables in Poland. During 2010-2014 growing seasons, 600 organic and 372 conventional samples were collected from certified farms. The vegetables were analyzed for aer...

Although many Pseudomonas syringae strains have already been determined, only a few genomes of strains belonging to pathovar lachrymans have been sequenced so far. In this study we report the genome sequence of P. syringae pv. lachrymans strain 814/98, which is highly virulent to cucumber. The genome size was estimated to be 6.58 Mb, with 57.97% GC...

Strains of Trichoderma are used in crop plant production as plant growth promoters and biological control agents. In this study, strains of T. atroviride and T. harzianum, overgrown on organic material, were applied as biopreparations to the soil in open-field lettuce cultivation, and their population levels were monitored over time. A newly develo...

This study was aimed to isolate, identify and characterize Pectobacterium spp. causing soft rot disease of cabbage and Chinese cabbage in Central Poland. Of fifty-two plant samples of cabbage and Chinese cabbage showing disease symptoms collected in Central Poland from 2007-2010, 542 bacterial isolates were obtained. Of isolates 117 caused soft rot...

Investigations were carried out in 2008-2010 on Chinese cabbage (Brassica rapa L. var. pekinensis (Lour.) Olson). The main problem in cultivation of this vegetable is physiological disorder – tipburn. It is connected with low level of calcium in young leaves and with water deficiency. In 2008, seeds of Chinese cabbage were sown twice, in April and...

Trichoderma strains that are beneficial to both the growth and health of plants can be used as plant growth-promoting fungi (PGPF) or biological control agents (BCA) in agricultural and horticultural practices. In order to select PGPF or BCA strains, their biological properties and taxonomy must be carefully studied. In this study, 104 strains of T...

Molecular markers that enable monitoring of fungi in their natural environment or assist in the identification of specific strains would facilitate Trichoderma utilization, particularly as an agricultural biocontrol agent (BCA). In this study, sequence analysis of internal transcribed spacer regions 1 and 2 (ITS1 and ITS2) of the ribosomal RNA (rRN...

Bacterial diseases of onion (Allium cepa L.) are serious problems in Poland. In this study bacterial strains were isolated from onion bulbs with soft-rot symptoms. Pathogenicity tests were conducted on onion bulbs and also on tobacco leaves on whose basis twelve isolates were chosen to further characterization. These isolates were identified using...

Numerous Trichoderma strains are beneficial to plants and can support plant growth and protect plants against stress factors, mainly pathogenic fungi. Consequently, Trichoderma are used as biocontrol agents in agriculture. Application of biocontrol Trichoderma strains to soil necessitates a suitable method to confirm the presence of these fungi dur...

In this study, the rep-PCR technique was used to differentiate isolates of bacteria belonging to genus Pseudomonas and phosphate-dissolving bacteria collected from the root vicinity of apple and sour cherry trees. DNA amplification was carried out with complementary primers for repetitive sequences: REP (repetitive extragenic palindromic sequence),...

Specific bacterial disease symptoms were observed on onion bulbs in almost all regions in Poland. For the purpose of identification of agents causing disease, bacteria were isolated from the symptomatic plants. Their pathogenicity was confirmed by using pathogenicity test on onion scales. These bacteria were identified biochemically and molecularly...

Interspecific somatic hybrids between a diploid potato clone DG 81-68 susceptible to Phytophthora infestans (Mont.) de Bary and a resistant diploid tuber-bearing species Solanum × michoacanum were generated and analyzed. About 30 regenerants displaying an intermediate morphology were obtained as a result of three separate PEG-mediated fusion experi...

The aim of work was to identify of bacteria causing pale brown or yellowish blotch of the cultivated mushroom. The effectiveness of disinfectants in growth reduction of pathogenic bacteria was also examined. The isolation of causal microorganism from mushroom caps with pale brown blotch was conducted. Biochemical, pathogenicity assays and 16S rRNA...

Antagonistic properties of four isolates of Trichoderma against fungal pathogen Rhizoctonia solani were investigated. The addition of T. harzianum strain PBG to the potground protected cucumber plants against root rot caused by R. solani and increased the weight of cucumber plants growing in potground with pathogen by 19-26%, compared to infested c...