Michail Orfanoudakis- PhD
- Professor (Associate) at Democritus University of Thrace
Michail Orfanoudakis
- PhD
- Professor (Associate) at Democritus University of Thrace
About
62
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Introduction
Mycorrhizal symbiosis
Current institution
Publications
Publications (62)
Στην παρούσα εργασία μελετήθηκε η μυκορριζική εξάρτηση ως δείκτης επίδρασης των μυκορριζών στην ανάπτυξη αγρωστωδών που εγκαταστάθηκαν σε μητρικό υλικό, προερχόμενο από την αποσάθρωση παραγνεύσιου. Για το σκοπό αυτό χρησιμοποιηθήκαν δέκα φυτοδοχεία όπου σπάρθηκε Festuca arundinacea. Στα πέντε από αυτά εφαρμόστηκε μυκητοκτόνο και στα υπόλοιπα έγινε...
Arbuscular mycorrhizal fungi (AMF) have become attractive as bio stimulants in agriculture due to plant nutrient uptake enchantment and stress tolerance [...]
Μυκόρριζα είναι η αμοιβαία επωφελής συμβίωση ανάμεσα στα φυτά και μύκητες που αποικίζουν τις ρίζες τους και προσφέρουν στα φυτά σημαντικά πλεονεκτήματα όσον αφορά την ανάπτυξη, τη λήψη θρεπτικών συστατικών από το έδαφος, την ανθεκτικότητα σε αβιοτικές και βιοτικές καταπονήσεις, καθώς και την απόδοση. Σκοπός της παρούσας εργασίας ήταν να μελετηθεί η...
The COFORMIT research project assesses the contribution of tree plantations at restored areas to the mitigation of climate change. Forests and plantations, through photosynthesis, absorb and store CO2, which is the main greenhouse gas in the atmosphere. Carbon is stored in the above and belowground biomass, the deadwood, the litter and the carbon s...
The contribution of litterfall (dead leaves, twigs, etc., fallen to the ground) and forest floor (organic residues such as leaves, twigs, etc., in various stages of decomposition, on the top of the mineral soil) is fundamental in both forest ecosystem sustainability and soil greenhouse gases (GHG) exchange system with the atmosphere. The effect of...
Inoculation with beneficial microbes represents a promising solution for sustainable agricultural production; however, knowledge on the effects of inoculants on the indigenous microbial communities remains limited. Here, we evaluated the impact of the arbuscular mycorrhizal fungus Rhizophagus irregularis and the promoting rhizobacterium Bacillus su...
Η ανάπτυξη θυσανωδών μυκορριζών αποτελεί μια συμβίωση μεταξύ μυκήτων του φύλου Glomeromycota (Θυσανώδεις Μυκορριζικοί Μύκητες - ΘΜΜ) και χερσαίων φυτών. Η παρούσα εργασία μελετά την συμβίωση ανάμεσα σε ΘΜΜ και 13 γενοτύπους καλαμποκιού (Zea mays L.) που καλλιεργήθηκαν σε κυψελωτή διάταξη, σε δύο στάδια ανάπτυξης (πλήρης άνθηση και γέμισμα σπόρων)....
The COFORMIT research project assesses the contribution of tree plantations at restored areas to the mitigation of climate change and to the improvement of air quality. Forests and plantations, through photosynthesis, absorb and store CO2, which is the main greenhouse gas in the atmosphere. Carbon is stored in the above and below-ground biomass, th...
Terrestrial plants establish symbiotic relationships with Arbuscular Mycorrhizal Fungi (AMF) of the phylum Glomeromycota. AMF symbiosis facilitates water and nutrient uptake resulting in increased yields in agricultural plants. There is mounting evidence that plants display distinct interactions with different AM fungi, so that plant growth and/or...
Red wood ants build their nests as above-ground mounds by collecting organic
materials from the forest floor. The effects of ant nest to the soil carbon and nitrogen along
with the soil pH, were measured. There was an increase in pH with distance from the nests
while there was a decrease in C and N concentration with the distance from the nest
incr...
Plant species effect on microbial activity of a limestone quarry with
pyrolusite’s byproducts mix as cover was investigated. For the remediation project three plant species: pine, cypress, and broom were
planted at 9 randomized plots. Microbial biomass (Cmic), basal respiration
(BR), alkaline phosphatase activity (ALP), along with microbial quot...
Arbuscular mycorrhizal (AM) symbiosis is an obligatory symbiosis among soil born fungi from the phylum Glomeromycota and terrestrial plants. AM symbiosis is well documented in several agricultural systems. However, such data is scarce at the honeycomb experimental design, which ensures the absence of competition and can be a powerful tool for plant...
The global average surface temperature is determined by the energy balance of the Earth, which is affected by, among other factors, gases that can absorb or reflect either solar radiation or the radiation from the Earth’s surface. Greenhouse gases (GHGs) in the atmosphere absorb radiation emitted from the Earth’s surface and re-emit that radiation...
Στην παρούσα εργασία μελετήθηκε η επίδραση της βλάστησης στη σταθερότητα των συσσωματωμάτων (ΣΣ) ενός μίγματος κάλυψης λατομικού χώρου ασβεστόλιθου που αποτελείτο κυρίως από παραπροϊόντα πυρολουσίτη. Στον υπό ανάπλαση χώρο φυτεύτηκαν τρία δασικά είδη (πεύκο, κυπαρίσσι και σπάρτο). Στη διάρκεια του πειράματος (3,5 έτη) η ΣΣ στη ριζόσφαιρα των φυτικώ...
Phylogenetic analyses of 18S–ITS–28S nuc rDNA sequences indicated that the arbuscular mycorrhizal fungus originally described as Glomus tortuosum and later transferred to the genus Corymbiglomus represents a separate, previously unrecognized clade at the rank of genus in the family Diversisporaceae (order Diversisporales, phylum Glomeromycota). The...
Τα κόκκινα δασικά μυρμήγκια φτιάχνουν τις φωλιές τους πάνω από την επιφάνεια του εδάφους συγκεντρώνοντας οργανικά υλικά από το δασικό τάπητα. Στην παρούσα έρευνα συγκρίναμε τις χημικές ιδιότητες του εδάφους στο κέντρο των φωλιών και του υπέργειου τμήματος τους με τον υπερκείμενο δασικό τάπητα και το έδαφος. Για τον σκοπό αυτό στην περιοχή μελέτης τ...
Honeycomb mass selection method dictates that plants should be arranged in a plot widely apart so as to eliminate any plant-to-plant interference for resources (i.e. nil-competition). Nil-competition allows for maximum phenotypic expression, such as maximum yield per plant, but also maximum phenotypic differentiation thus revealing the greatest pos...
Different plant species, water regimes and microbes in the rhizosphere might shape rhizosphere microbial communities due to their effects on root exudation patterns and interactions. In this study, we investigated whether rhizosphere microbial communities have distinct structures according to plant type ( Festuca pratensis , Dactylis glomerata or a...
The intensity of thinning has an impact on annual litterfall production in coniferous forests. The knowledge of thinning impacts on forest mass and litterfall production and thus, their correlation with soil–atmospheric GHG fluxes exchange in the Mediterranean region remains limited.The aim of this study was i) to quantify the spatial and temporal...
Knowledge of the response of thinning implementation on forest soil–atmospheric greenhouse gas (GHG) (CO2, CH4, N2O) fluxes exchange system in Mediterranean region is limited because of the high heterogeneity of both soil properties and forest biomass. The novelty of this study is grounded predominantly in evaluating for the first time the response...
Efficiency in product yield and purity when isolating microbial DNA from soil could be a critical factor for several molecular analyses. Progressively, traditional extraction protocols are being replaced by effective commercial soil DNA extraction kits, however, it has been shown that differences in soil properties and microbial population composit...
The importance of beneficial microorganisms in soil structure and plant development is known for many years. Especially arbuscular mycorrhizal fungi (AMF) constitute one of the most significant groups of soil microorganisms because of their ability to provide plants with nutrients, such as phosphorus and other immobile elements and improve plant gr...
In Mediterranean ecosystems an increasing demand for in situ trace gas exchange data is emerging to enhance the adaptation and mitigation strategies under forest degradation. Field-chamber green-house gas fluxes and site characteristics were analysed in two Mediterranean peri-urban pine forests showing degradation symptoms. We examined the effect o...
Phytoremediation was used to regenerate a limestone quarry area. Plant growth mixed medium added over the quarry surface, consisting of a mixture of pyrolusite byproducts, natural soil, sand, and rice husk. Three different plant species: pine, cypress, and broom were planted at 9 randomized plots in order to assess the effects of vegetation on the...
Several studies have attempted to clarify the effect of plant essential oils on microorganisms in vitro. The specificity of mycorrhizas lies in the fact that it must be carried out in vivo. The aim of this study was to explore the effect of aromatic Lavandula stoechas L. degradation on development of two mycorrhizal species Septoglomus deserticola...
The arbuscular mycorrhizal fungi (AMF) and the essential oils are both agents of sustainable agriculture, and their independent effects on the community of free-living soil microbes have been explored. In a tomato pot experiment, conducted in a sandy loam mixture, we examined the independent and joint effects of inoculation with the fungus Rhizopha...
Στην παρούσα εργασία μελετήθηκε η επίδραση της βλάστησης στις φυσικές, χημικές και βιολογικές ιδιότητες μίγματος κάλυψης λατομικού χώρου ασβεστόλιθου που αποτελείτο σε ποσοστό70% από παραπροϊόνταπυρολουσίτη. Για το σκοπό αυτό στον υπό ανάπλαση χώρο φυτεύτηκαν τρία δασικά είδη: πεύκο, κυπαρίσσι και σπάρτο. 3,5 χρόνια μετά την φύτευση, η μικροβιακή β...
The issue of high concentration of salt in soil is not restricted to coastal areas, but also expands to cultivated lands, complicating or even intercepting the growth of plants. The objective of this paper is to study the influence of zeolite, effective microorganisms (EM), arbuscular mycorrhizal fungi (AMF) and seaweed extract on apple plants MM10...
Limited information is available about the selection of effective mycorrhizae and the exploitation of their beneficial effects on the enhancement of the forage production of Medicago sativa under the predicted warmer and drier conditions in the Mediterranean region the following years. The objective of this study was to test the effects of Rhizopha...
Abstract. Legumes are particularly important feed sources for livestock due to their high nutritive value. The
objective of this study was to examine the agronomic phosphorus use efficiency (PUE) of Trifolium
subterraneum at different seeding rates (13, 17, 20 kg ha-1) and phenological stages (inflorescence,
fruiting). The experiment was conducted...
Για την ανάπλαση λατομικού χώρου ασβεστόλιθου χρησιμοποιήθηκε υποστρώμα κάλυψης που αποτελείτο από μίγμα παραπροϊόντων πυρολουσίτη (MnO2). Στον υπό κάλυψη χώρο φυτεύτηκαν τρία δασικά είδη πεύκο, κυπαρίσσι και σπάρτο. Σκοπός της μελέτης αυτής ήταν να μελετηθεί η ανάπτυξη της βιολογικής δραστηριότητας στο μίγμα κάλυψης. Ως μάρτυρας (Μ) χρησιμοποιήθηκ...
Η ξηρασία αποτελεί την πιο σημαντική περιβαλλοντική καταπόνηση για τα φυτά, καθώς επηρεάζει την αύξηση και την ανάπτυξή τους. Για το λόγο αυτό έχουν πραγματοποιηθεί πολλές προσπάθειες, ώστε να βελτιωθεί η παραγωγικότητα των καλλιεργειών σε συνθήκες μειωμένης άρδευσης. Στην εργασία αυτή μελετήθηκε η επίδραση λιπάσματος, ζεολίθου, μυκορριζών και ενερ...
Legumes are important feed source for small ruminants due to their high nutritive value. The objective of this study was to assess the effects of phosphorous fertilization (40 kg ha-1 and control) and seeding rate (20, 17 and 13 kg ha-1) on forage production and the nutritive value of Trifolium subterraneum collected at two phenological stages (ear...
Tree growth, understorey characteristics and soil parameters were evaluated in a silvopastoral system in northern Greece at seven and twelve years after its establishment. The experiment was laid out in 1992 and included three understorey treatments (grass, legume and control-unsown), two tree species-sycamore (Acer pseudoplatanus L.) and Scots pin...
Η εύρεση κατάλληλης ποιότητας εδάφους για εδαφοκάλυψη αποτελεί ένα από τα μεγαλύτερα προβλήματα της αποκατάστασης των ισχυρά διαταραγμένων από τη λατομική δραστηριότητα εδαφών. Το πρόβλημα γίνεται ιδιαίτερα οξύ στις περιπτώσεις στις οποίες η δραστηριότητα αυτή ασκείται σε ορεινές και ημιορεινές περιοχές. Λόγω του προβλήματος αυτού καταβάλλεται διεθ...
Tree growth, understorey characteristics and soil parameters were evaluated in a silvopastoral system in northern Greece at seven and twelve years after its establishment. The experiment was laid out in 1992 and included three understorey treatments (grass, legume and control- unsown), two tree species - sycamore (Acer pseudoplatanus L.) and Scots...
Limited information is available regarding the selection of effective mycorrhizae and the exploitation of their beneficial effects on the enhancement of the forage production of Dactylis glomerata under the predicted warmer and drier conditions in the Mediterranean region. The objective of this study was to test the effects of Glomus intraradices,...
Abstract Phylogenetic analyses of SSU-ITS-LSU nrDNA sequences and morphological studies of spores and mycor- rhizae confirmed our supposition of finding two new spe- cies of arbuscular mycorrhizal fungi of the genus Septoglomus in the phylum Glomeromycota. Morphologi- cally, the first species, named S. jasnowskae, is distin- guished by its pale yel...
Understanding how the management practices of silvopastoral systems affect the long-term sustainability of oak ecosystems and what their influence is on nutrient cycling and plant community, is of great interest. The aim of this study was to examine the effects of relative grazing intensity on soil properties and on vegetation characteristics in an...
Rooted leafy cuttings of three Greek olive (Olea europaea L.) cultivars (Koroneiki, Kothreiki and Chondrolia Chalkidikis) were grown for six months in three soil types, in an experimental greenhouse, in order to investigate: i) if their root system was colonized by arbuscular mycorrhiza fungus (AMF) genus and, ii) if genotypic differences concernin...
Mine tailings -- the waste rock produced in the mining process – pose a serious environmental hazard in parts of the Halkidiki peninsula, Greece. Native plant species, such as Nerium oleander are used for the phytoremediation of areas covered by dried tailings and the filter cake produced by the implementation of modern froth-flotation methods. The...
Alnus glutinosa is an important pioneer species that forms effective symbioses with Frankia and ecto and arbuscular mycorrhizal fungi (AMF). There is evidence that Frankia and AMF interact and the focus of this study was to investigate how interactions affected root system and root hair development. A. glutinosa seedlings were grown in pots in soil...
The trace element analysis of Aleppo pine (Pinus halepensis Mill) as a function of tree age, was achieved by the tree-ring sampling method, in a polluted region of Attica, Greece. The elements quantitatively determined for the last 140 years by atomic spectrophotometry analysis were: Cu, Fe, Zn, Mn and Pb. With the exception of Pb, all other elemen...
Soil characteristics of Pseutotsuga menziensii, Thuja plicata, Pinus sylvestris and Pinus ponterosa 50 year old exotic plantation, were studies and compared with those of a natural nearby Fagus moesiaca stand, in order to determine the ecological cost of the exotic species use. The pH, the concentration and accumulation of the N, P, Ca, K, Mg, C, C...
Glasshouse experiments showed significant differences in early interactions of Alnus glutinosa with five different arbuscular mycorrhizal species and Frankia. Thirty and 60 days after inoculation of roots with AMF alone, colonisation was highest with Glomus hoi and G. mosseae followed by Gigaspora rosea. Lowest colonisation was with Acaulospora scr...
