Michail Michaylov

Michail Michaylov
Russian Academy of Medical Sciences | RAMS

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106
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Publications

Publications (106)
Article
Within the last decade, a large number of viruses genetically related to human hepatitis viruses have been identified in different animal species, including monkeys. Numerous viruses related to human hepatitis A virus (HAV, Picornaviridae: Hepatovirus: Hepatovirus A) were detected in various mammalian species in 2015–2018, predominantly in bats and...
Article
Rationale. Hepatitis E (HE) is a zooanthroponosis. Domestic pigs are the main reservoir for hepatitis E virus (HEV) in the Republic of Belarus (RB). Considering the well-developed pig farming, there is a high risk of infection spread among the population; however, the scale of virus circulation and patterns of HE epidemiology in the above region ar...
Article
Hepatitis D (delta, δ) virus (HDV) was discovered more than 40 years ago, but the understanding of its origin and evolution is poor. This is mainly due to the lack, until recently, of data on the existence of any viruses similar to HDV. The discovery in recent years of sequences of new delta-like agents in a wide range of vertebrate (Vertebrata) an...
Article
Introduction: Viral hepatitis E is a zooanthroponotic disease that occurs in humans and various animals, including monkeys. It is caused by hepatitis E virus (HEV) (Hepeviridae, Orthohepevirus: Orthohepevirus A), for which 8 genotypes have been described to date. Among them, strains of genotypes 1 and 2 have been isolated from humans, strains of g...
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The problem of choosing a strategy to control hepatitis A in the Russian Federation remains relevant for the health care of our country. By strategy we mean a scientifically based program of action that defines the priorities and resources needed to effectively control hepatitis A. The aim of the work is to present a strategy to control hepatitis A...
Article
Introduction: Hepatitis D (delta, 5) is caused by an RNA virus (hepatitis D virus, HDV) from genus Deltavirus, and is the most severe and difficult to treat disease among both viral hepatitis and infectious diseases in general. The development of HDV infection in the host organism is possible only in the presence of hepatitis B virus (HBV). Coinfe...
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The species of the genus Dirofilaria are filarial parasites causing zoonotic infections in humans with an increasing incidence in temperate and tropical areas of the world. Due to its classification as a vector-borne disease, the most important factors influencing dirofilariosis transmission are those related to climate, such as temperature and hum...
Article
Introduction: Hepatitis E (HE) is an important public health problem worldwide and is especially significant for pregnant women, among whom the associated mortality rate reaches 25%. The distribution of HE serological markers in this cohort in the endemic regions of Central Asia is poorly understood. The aim of the study was to assess the seroprev...
Article
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Introduction: Belgorod region is the territory with the highest incidence of hepatitis E in the Russian Federation. Objectives: The aim of the study was to comprehensively characterize the circulation of hepatitis E virus (HEV) in the Belgorod region, including the study of population immunity to the virus, determining the prevalence of infectio...
Article
Hepatitis A is a widespread viral infection. The HAV strains of “human” and “monkey” origin are similar in their morphological and antigenic properties, but differ genotypically. The aim of this research was a comparative study of serological and molecular-genetic markers of HAV infection in monkeys born at the Adler Primate Center and in those imp...
Article
Despite the fact that the Kyrgyz Republic (KR) belongs to the highly endemic regions of the world for hepatitis E, the true extent of the spread of this infection in the country remains poorly understood. It was estimated the prevalence of serological markers of hepatitis E virus (HEV) infection among patients with acute viral hepatitis (AVH) from...
Article
The Hepatitis E (HE) is a viral liver disease that is common on all continents at different rates and very frequently is not symptomatically distinguishable from other viral hepatitis, such as hepatitis A, B and C, as well as from other infectious diseases referred to as "acute jaundice syndrome". The GE-anthroponosis (in the case of infection caus...
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Aim . The development of the hepatitis E virus (HEV) genotype 3 recombinant capsid protein. Materials and methods . E.coli strains, plasmid vectors, serological and clinical samples, ELISA reagent kits, molecular biological, bioinformatic, biotechnological, biochemical and serological methods. Results . Using viruscontaining material from pigs of B...
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This article presents the results of a study of the epidemiology of hepatitis E in the Russian Federation. Obtained data explaining the phenomenon, known as the Balayan paradox — the wide distribution of anamnestic antibodies to the hepatitis E virus in the absence of a registered incidence. It was shown that imported cases of infection are not abl...
Article
Data on natural HEV infection of infection in monkeys are limited. We report a case of hepatitis E virus genotype 4 infection in captive non-human primates (cynomolgus monkeys) imported from Vietnam. Phylogenetic analysis demonstrated that HEV infection was not the result of spillover from single source of infection, but rather the persistent circu...
Article
Aim. To determine the prevalence of amino acid substitutions in hepatitis C virus NS5a and core proteins which are associated with resistance to direct-acting antivirals and interferon in genotype 1b (HCV-1b) strains circulating in Russia. Materials and methods. Nucleotide sequences of NS5a (n=93) and core (n=30) of HCV-1b were obtained using direc...
Article
Full-text available
Aim. Design аис1 construction of the hepatitis E virus (HEV) genotype 3 full-size ORF3 recombimnt polypeptide. Materials and methods. Escherichia coli strains, ptasmid vectors, serologiral and biological amples, molecular biological, bioinformatic, biotechnological, biochemical and serological methods.Results. RNA was isolated from pig fecal extrac...
Article
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Amino acid substitutions R70Q/H and L91M in HCV subtype 1b core protein can affect the response to interferon and are associated with the development of hepatocellular carcinoma. We found that the rate of R70Q/H in HCV 1b from Russia was 31.2%, similar to that in HCV strains from Asia (34.0%), higher than that in the European (18.0%, p=0.0010 ), bu...
Data
Table S2: presentation by HLA I alleles of 9-mer peptide variants derived from the region between aa 83 and 99 of the nucleocapsid (core) of HCV 1b encompassing aa 91, predicted using EPISPOT tool (http://bio.med.ucm.es/episopt.html; EPISPOT [45]). Nine amino acid long peptides were chosen based on the consensus amino acid sequence of nucleocapsid...
Data
Figure S1: geographical origins of samples of hepatitis C patients infected with HCV 1b from the Russian Federation used in the analysis of frequency of substitutions in HCV core amino acid positions 70 and 91. Data on the sample set is presented in Table 2.
Data
Table S1: presentation by HLA I alleles of 9-mer peptide variants derived from the region between aa 62 and 78 of the nucleocapsid (core) of HCV 1b encompassing aa 70, predicted using EPISPOT tool (http://bio.med.ucm.es/episopt.html; EPISPOT [25]). Nine amino acid long peptides were chosen based on the consensus amino acid sequence of nucleocapsid...
Data
Figure S2: phylogenetic tree for the complete HCV core sequences covering nucleotide positions 342–915 according to HCV 1a reference strain H77 (GenBank accession number NC004102) built using a Bayesian likelihood-based algorithm; detailed overview of the tree fragments (А; В). Sequences of the Russian origins are underlined. Sequences with wild ty...
Data
Figure S3: T-cell epitopes in the consensus amino acid sequence of nucleocapsid (core) of HCV 1b encompassing amino acids 70 (A) and 91 (B) localized using Immune Epitope Database and Analysis resource (http://www.iedb.org).
Data
Table S3: NetMHCIIpan 3.1 engine [48] predicts only weak binding of HCV 1b core peptides encompassing amino acid residue 91 to a limited number of human HLA class II molecules. Peptide predicted to be recognized is boxed in green and aa position 91 in red. Strong binding is characterized by binding level < 2%; weak binding, 2 to 10%; no binding, >1...
Article
The purpose of the study was to determine the duration of antibody response against hepatitis E virus (anti-HEV). Veterans of the war in Afghanistan who were in this endemic region in the late 1970s and early 1980s were tested for anti-HEV. On average, 20 years after the end of military service in Afghanistan, the rate of seropositivity was 30.0% (...
Article
Full-text available
Aim. The development of the hepatitis E virus (HEV) genotype 1 recombinant capsid protein. Materials and methods. Escherichia coli strains, plasmid vectors, serological and clinical samples, ELISA reagent kits, molecular biological, bioinformatic, biotechnological, biochemical and serological methods. Results. Using HEV genotype 1 DNA copy of subge...
Article
Full-text available
Aim. The development of the hepatitis E virus (HEV) genotype 1 full-size ORF3 recombinant polypeptide. Materials and methods. Escherichia coli strains, plasmid vectors, serological and clinical samples, ELISA reagent kits, molecular biological, bioinformatic, biotechnological, biochemical and serological methods. Results. HEV genotype 1 RNA had bee...
Article
Full-text available
Aim. Experimental reproduction of HEV-infection in mini-pigs and comparative analysis of virological and immunological characteristics of experimental infection. Materials and methods. Wiesenauer minipigs (2 females and 4 males, age 50 - 60 days, weight 5-10 kg) were infected by HEV genotype 3 strain isolated from swine feces in Belgorod region in...
Article
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Aim. To determine of the prevalence of viral hepatitis A, E, B, C and D markers in migrant workers.Materials and methods. Blood serum samples from 1,333 migrant workers recently arrived in Russia from Uzbekistan (n = 464), Tajikistan (n = 415), Ukraine (n = 308) and Moldova (n = 146) were analyzed. Anti-HAV IgG, anti-HEV IgM and IgG, HBsAg, anti-HB...
Article
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Aim. To determine the prevalence of hepatitis C virus (HCV) in different age groups of the conditionally healthy population of Russia.Materials and methods. Total 4764 serum samples from a conditionally healthy population of five regions of Russia (Moscow, Rostov, Sverdlovsk regions, Tyva Republic, and Sakha Republic (Yakutia) were tested. The stud...
Article
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Aim. Determine spread of hepatitis C vims genotypes, circulating in Moscow Region over the last decade. Materials and methods. The presence of HCV RNA, genotype and subtype of the vims were determined in blood sera samples obtained in 2006 - 2014 from 2847 individuals with the presence of HCV infection, who had or did not have injectable dmg admini...
Article
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Serum from humans (n = 646) and monkeys (n = 1867) collected during the period 1999-2013 was tested by enzyme immunoassay. Anti-HEV IgG was detected significantly more frequently (P > 0.001) in rhesus macaques (Macaca mulatta) - 45.1 ± 1.6% (n = 1001) than in cynomolgus macaques (M. fascicularis) 16.2 ± 1.8% (n = 426). Single seropositive individua...
Article
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Aim: Description of the first documented case of imported hepatitis E, associated with geno- type 4 of HEV and introduced from southern France. Materials and methods: Clinical, epidemio- logic and laboratory analysis of the imported case of disease of hepatitis E was carried out. Phylogenetic analysis of nucleotide sequences of HEV isolate, take...
Article
Full-text available
Methods: 6,217 sera samples collected from volunteers in six epidemiologically different regions of Russia were tested for serological and molecular markers of HBV infection. A mathematical model developed by the U.S. Centers for Disease Control and Prevention was used to estimate the effect of vaccination and birth dose coverage on the incidence...
Article
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We developed a model of hepatitis E virus infection in common marmosets (Callithrix jacchus) and determined optimal route of infection, duration, clinical and virological characteristics of infection in laboratory animals. Using this model, we demonstrated that replication of hepatitis E virus primarily occurs in the liver, while virus replication...
Article
The study was carried out to develop approaches to standardization of laboratory diagnostic of hepatitis E. The three stages of standardization are establishment of analytical sensitivity of molecular test for detection of RNA of virus of hepatitis E; establishment of analytical sensitivity in International Units of enzyme-linked immunosorbent assa...
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Резюме Что стоит за бессимптомным повышением активности аминотрансфераз? Путь к окончательному диагнозу в таких ситуациях весьма сложен. Определение лишь двух маркеров инфицирования вирусами гепатитов – HBsAg и anti-HCV, принятое в общей клинической практике, и сужение показаний к проведению биопсии печени значительно ограничивает диагностические в...
Article
Hepatitis delta (HD) is characterized by rapid progression to fibrosis, and development of hepatocellular carcinoma, and a high mortality rate. The article presents data on the epidemiology, diagnosis, treatment of HD. The views of the epidemiological, clinical and virological characteristics of HD-infection among population of the Russian Federati...
Article
Frequency of detection determination for past and current hepatitis E virus (HEV) infection markers in children with immune suppression, as well as children with normal immune status. The presence of HEV markers (anti-HEV IgG and IgM, HEV RNA) was studied in 609 sera samples of children with neurologic pathologies, 87 samples--from children with im...
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AIM: Viruses from genus Anelloviridae (TTV, TTMDV and TTMV) are small DNA viruses that are widespread in human popu- lation. Data on tissue tropism, cell localization and morphometry of anelloviruses are scarce. The purpose of this study was to determine the prevalence of TTV, TTMDV and TTMV in persons with liver disease and in healthy individuals,...
Article
AIM: Clinical and virological characteristic of hepatitis delta familial clusters in region of Russia that is endemic for this infection (Republic Tuva).
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Data about efficacy of conducting of routine vaccination of children against Hepatitis A are presented in the article. The results of realization such strategy of vaccine prevention of Hepatitis A in selected regions of Russian Federation are evaluated. The perspectives of using this experience in other regions of the country is discussed.
Article
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Enterovirus 71 (EV71) is an emerging human pathogen causing massive epidemics of hand, foot and mouth disease with severe neurological complications in Asia. EV71 also circulates in Europe, however it does not cause large outbreaks. The reason for distinct epidemiological patterns of EV71 infection in Europe and Asia and the risk of EV71 epidemic i...
Article
A review of recent publications on epidemiology and seroepidemiology of enterovirus type 71 in various regions of the world and authors' own results of study of seroepidemiology and molecular epidemiology of EV71 in Russia are presented.
Article
The virucidal action of solvent tributyl phosphate and detergent sodium cholate used in the production of immunoglobulin for inactivation of viruses with lipid envelope was studied on the model of duck hepatitis B virus. PCR analysis revealed no significant decrease in duck hepatitis B virus DNA concentrations after treatment with solvent/detergent...
Article
Aim: Carry out retrospective clinical-epidemiological analysis of sporadic and group cases of acute HE morbidity in St. Petersburg (2000 - 2012). MATERIALS AND METHODS; Medical histories of 11 patients with sporadic morbidity (9 males and 2 females, average age 36 +/- 18) and 13 patients involved in group HE morbidity were analyzed. Acute hepatiti...
Article
Full-text available
The aim of the investigation was to study the prevalence of hepatitis B virus (HBV) variants with polymerase gene mutations among HBV-infected and HBV/HIV-coinfected patients previously received no nucleotide/nucleoside analogues therapy. Materials and Methods. We studied the blood serum samples of 459 patients with HBV-monoinfection and 590 HIV-i...
Article
Full-text available
With improved HCV therapy, challenges regarding HCV diagnosis, such as seronegative window period, false positive readings, and differentiation between recent, chronic, and resolved infections, are of increasing importance. To address these challenges an innovative device—SMARTube HIV & HCV—was used. Blood samples were tested for anti-HCV antibodie...
Article
The detection of HBsAg in blood serum using immune-enzyme analysis techniques decisively matters both for diagnostics of acute and chronic hepatitis B and screening of donor's blood and its components, controls of persons from risk groups of hepatitis B injection. The making of panels containing wide specter of samples of blood serums with sero-var...
Article
Aim: Evaluate the effect of mass vaccination against hepatitis B implemented within the framework of national project Health on the rate of development of unfavorable outcomes of infection and on average lifetime of the population of Russia. Materials and methods: Hepatitis B markers (HBsAg, anti-HBc, HBeAg, anti-HBe) were determined by enzyme i...
Article
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593 patients with chronic viral hepatitis were tested for HGV-RNA and TTV DNA of. HGV-RNA detected in 3 times less than TTV DNA - in 13.6% and 46.64%, respectively. A comparative evaluation of clinical and biochemical parameters in patients with coinfection HCV/HGV and HCV monoinfection revealed significant differences in the average level of activ...
Article
The mechanisms of development of autoimmune diseases may be associated with a complex of genetic, immune, hormonal, and infectious factors. Autoimmune diseases include a wide range of systemic and organ-specific diseases, including autoimmune hepatitis (AIH). It is currently assumed that the pathogenesis of AIH is due to compromised immune regulati...
Article
Aim: Analysis of hepatitis A (HA) incidence in Tyva Republic in 2008 exploiting traditional epidemiological and molecular methods. Materials and methods: Epidemiological analysis of HA cases and contact persons in Erzinsky and Kyzyl regions of Tyva Republic was performed. Class M and G antibodies to HAV were determined in serum samples (n = 28),...
Article
In 1970s enterovirus type 71 (EV71) caused several epidemics of poliomyelitis-like disease with severe neurologic sequelae. In the last 20 years EV71 was the cause of series of outbreaks and epidemics of foot-and-mouth disease-like conditions with neurologic sequelae in countries of South-East Asia. During the last epidemic of EV71 infection, which...
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Full-text available
Part of the Federal System of External Quality Assessment of Clinical Laboratory Tests named PCR-identification of hepatitis C virus (HCV) and aimed at detection of HCV RNA by polymerase chain reaction (PCR) assay is functioning from 2000. Ninety, 98, and 112 laboratories from more than 50 regions of Russian Federation participated in it in 2000,...
Article
Two tests for measurements of hepatitis B virus DNA in human serum and plasma, VERSANT HBV 3.0 (based on the use of branched DNA chains) and Biotitre-B (a real-time PCR variant), were compared. Serum specimens from patients (n=56) with documented viral hepatitis were tested. For specimens with DNA values in the linear range in both tests the correl...
Article
The virus inactivation in Methylene-blue treated fresh plasma was studied. Plasma processing was made by Macotronic device ("Macopharma S.A.", France). The absence of appreciable effects on plasma biochemistry was confirmed by coagulological and chromatographic analyses of the samples before and after virus inactivation. The efficiency of virus ina...
Article
A total of 3194 cases of enterovirus meningitis were notified in the Russian Federation in 2005, of them there were 1434 cases in the Khabarovsk Territory. Enteroviruses were isolated from 1020 out of the virologically studied 1362 patients from the Khabarovsk Territory. Viruses E6 and E30 were isolated in 80 and 14.7% of cases, respectively. E1, E...
Article
As revealed in the present survey, during the last 3 years, against a background of decreased number of registered cases of acute hepatitis B (HB) and acute hepatitis C (HC), an increase in the proportion of patients with the chronic forms of these diseases was observed. The incidence rate of carriership of hepatitis B (HBV) and hepatitis C viruses...
Article
Certain specific features of the present epidemic situation with hepatitis B (HB) in Russia were established: significant growth of HB morbidity, starting from 1995; the prevalence of persons aged 15-29 years among HB patients, which was linked with the sharp activation of the sexual route of the transmission of HB virus in recent years; an essenti...
Article
Anti-HVA IgM, HBsAg, anti-HBc IgM, anti-HVC were determined in the blood sera and saliva specimens obtained from patients with acute hepatitis A, B, C (HA, HB, HC) and from healthy persons of the control group. 37 HA patients, 27 HB patients and 24 HC patients, observed in the dynamics of the disease (for a period of 1 month and longer from the app...
Article
To determine the present-day specific clinical features of viral hepatitis A (HA) in children, observation on 447 children aged 1 year and 10 months to 14 years with verified HA monoinfection was carried out. At the season of 1993 essential differences were observed in the clinical picture of HA: the disease took a more severe course with the preva...
Article
Antibody to hepatitis E virus of IgG class (anti-HEV IgG) is regularly detected in industrialized countries, where HEV is non-endemic, at levels not exceeding 2-3%; seropositive individuals are often found in certain groups of patients and professionals exposed to an increased risk of blood-borne infections. The present study was aimed at the ident...
Article
Increase in the number of seropositive subjects in the population of European and North-American regions not endemic for hepatitis E stimulated research in this field. This study was aimed at investigating the incidence of IgG antibodies to hepatitis E virus (anti-HEV-IgG) in subjects with different liver diseases and in groups at increased risk of...
Article
Increase in the number of seropositive subjects in the population of European and North-American regions not endemic for hepatitis E stimulated research in this field. This study was aimed at investigating the incidence of IgG antibodies to hepatitis E virus (anti-HEV-IgG) in subjects with different liver diseases and in groups at increased risk of...
Article
32 children on the treatment for acute leukemia and aplastic anemia with hepatic dysfunction have been screened for hepatitis B and C viruses. 16 children (50%) had toxic hepatitis, 14 (44%) viral hepatitis B, 2 (6%) viral hepatitis C. All the examinees have undergone blood transfusions. Among the patients 3 children completed the treatment and wer...
Article
Hepatitis E is a major cause of acute icteric disease widespread in tropical and sub-tropical regions but rarely occurring in industrialized countries. Recently solid-phase enzyme immunoassays with recombinant antigens have been introduced for diagnosis of this infection. The aim of this study was to evaluate the diagnostic potential of a newly dev...