Michaela Jandová

Institute of Botany of the ASCR · Laboratoř molekulární cytogenetiky

Background The genus Allium is known for its high chromosomal variability, but most chromosome counts are based on a few individuals and genome size (GS) reports are limited in certain taxonomic groups. This is evident in the Allium sect. Codonoprasum , a species-rich (> 150 species) and taxonomically complex section with weak morphological differe...

Background The genus Allium is known for its high chromosomal variability, but most chromosome counts are based on a few individuals and genome size (GS) reports are limited in certain taxonomic groups. This is evident in the Allium sect. Codonoprasum, a species-rich (> 150 species) and taxonomically complex section with weak morphological differen...

Introduction Despite the wealth of studies dealing with the invasions of alien plants, invasions of alien genotypes of native species (cryptic invasions) have been vastly neglected. The impact of cryptic invasions on the biodiversity of plant communities can, however, be significant. Inland saline habitats and halophytes (i.e., salt-tolerant plant...

Background: CACTA transposable elements (TEs) comprise one of the most abundant superfamilies of Class 2 (cut-and-paste) transposons. Over recent decades, CACTA elements were widely identified in species from the plant, fungi, and animal kingdoms, but sufficiently studied in the genomes of only a few model species although non-model genomes can bri...

In the species-rich genus Impatiens, few natural hybrids are known, even though closely related species often occur sympatrically. In this study, we aim to bridge the gap between micro- and macro-evolution to disentangle pre- and postzygotic mechanisms that may prevent hybridization in the Impatiens purpureoviolacea complex from Central Africa. We...

The establishment and success of polyploids are thought to often be facilitated by ecological niche differentiation from diploids. Unfortunately, most studies compared diploids and polyploids, ignoring variation in ploidy level in polyploids. To fill this gap, we performed a large-scale study of 11,163 samples from 1,283 populations of the polyploi...

Satellite DNA (satDNA) is one of the major fractions of the eukaryotic nuclear genome. Highly variable satDNA is involved in various genome functions, and a clear link between satellites and phenotypes exists in a wide range of organisms. However, little is known about the origin and temporal dynamics of satDNA. The “library hypothesis” indicates t...

Extensive and complex links exist between transposable elements (TEs) and satellite DNA (satDNA), which are the two largest fractions of eukaryotic genome. These relationships have a crucial effect on genome structure, function and evolution. Here, we report a novel case of mutual relationships between TEs and satDNA. In the genomes of Chenopodium...

The genus Ficaria is a taxonomically intricate group in which polyploidization and hybridization contribute to taxa diversification. In central Europe, populations of diploid F. calthifolia and tetraploid F. verna subsp. verna occasionally come into contact, which results in an interspecific triploid hybrid, as recently demonstrated using molecular...

Satellite DNA (satDNA) is the most variable fraction of the eukaryotic genome. Related species share a common ancestral satDNA library and changing of any library component in a particular lineage results in interspecific differences. Although the general developmental trend is clear, our knowledge of the origin and dynamics of satDNAs is still fra...

Supplementary data 1. Occurrence of CficCl-61-40 satDNA family in genomes of Chenopodium diploid species revealed by RepeatExplorer pipeline and formations of high order repeat (HOR) units

Supplementary data 3. Repetitive elements selected for sequence characterization and in situ hybridization and primers used for amplification.

Reconstruction of the major part of the ancestral monomer BLAST-detected relatedness between minisatellite monomers of CficCl-61-40 satDNA family makes it possible to determine the major part of the ancient monomer. For this reconstruction for one RE cluster of C. bryobifolium (cluster 2) and for seven RE clusters C. vulvaria (clusters 28, 94, 112,...

Chromosomal distribution CficCl-61-40 satDNA family sequences. CficCl-61-40 is labelled red; C. acuminatum-specific HOR unit CacuCl-1-117 of 117 bp is labelled green.

N). For these monomers the sequence alignments containing the consensus sequence reconstructed by RE and sequences obtained by cloning and sequencing of clones are presented. For the cloned sequences identities with the consensus is represented by dots, differences by the alternative nucleotide characters. Tables contain the % of sequence similarit...

Allium pseudotelmatum, a new species of A. sect. Codonoprasum, is described from the southern Dalmatia (Croatia). It is a late-summer flowering species with pentaploid (2n = 40) chromosome complement, occurring in synanthropic habitats (Citrus orchards with nutrient rich and fresh moist soil). Karyology, leaf anatomy, morphology, ecology, molecular...

Aims. Many polyploids are geographically separated from their lower-ploidy progenitors at a variety of spatial scales. Diverse adaptive and non-adaptive mechanisms have been suggested to contribute to these spatial patterns. A long-standing hypothesis holds that polyploids are established and persist due to specialization to different ecological co...

Dark-induced growth (skotomorphogenesis) is primarily characterized by rapid elongation of the hypocotyl. We have studied the role of abscisic acid (ABA) during the development of young tomato (Solanum lycopersicum L.) seedlings. We observed that ABA deficiency caused a reduction in hypocotyl growth at the level of cell elongation and that the grow...

Background and AimsAlthough the large variation in genome size among different species is widely acknowledged, the occurrence and extent of variation below the species level are still controversial and have not yet been satisfactorily analysed. The aim of this study was to assess genome size variation in six ploidy levels (2n = 3x-8x) of the polypl...

Light is one of the most important factor influencing plant growth and development all through their life cycle. One of the well-known light-regulated processes is de-etiolation, i.e. the switch from skotomorphogenesis to photomorphogenesis. The hormones cytokinins (CKs) play an important role during the establishment of photomorphogenesis as exoge...

Expression by qRT-PCR of SlCYCD3;1 (A) and SlCYCD3;3 (B) in 2-do etiolated seedlings of cv. Rutgers and 7B-1 mutant subjected for 24 additional hours to the D or BL. (TIF)

Expression by qRT-PCR of SlCKX1 (A) and SlCKX5 (B) in 2-do etiolated seedlings of cv. Rutgers and 7B-1 mutant subjected for 24 h to the D or BL. (TIF)

Expression by qRT-PCR of SlLOG1 (A), SlLOG2 (B), SlLOG4 (C) and SlLOG6 (D) in 2-do etiolated seedlings of cv. Rutgers and 7B-1 mutant subjected for 24 h to the D or BL. (TIF)

The spatial distribution of cytotypes can provide valuable insights into the evolution of polyploid complexes. Previously, only tetraploid Allium oleraceum was reported from Slovakia. Analysing 863 individuals from 93 populations from Slovakia revealed an extensive variation in theDNAploidy levels of Allium oleraceum (3x, 4x, 5x and 6x). Of the mai...