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Michael Sandholzer

Michael Sandholzer
Dental Industry

M.Sc. (Vienna) Ph.D. (Birmingham)

About

34
Publications
93,251
Reads
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391
Citations
Introduction
After working on heat-induced and mechanical alterations of dental tissues during my M.Sc. and Ph.D., my postdoctoral work focused on various in-vivo and microscopic imaging methods (rodent development, disease models of lung and mesothelium). Now working in medical device industry, focusing on dental imaging.
Additional affiliations
August 2016 - present
Dental Industry
Position
  • Business Development Manager
April 2015 - July 2016
Helmholtz Zentrum München Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH)
Position
  • 3-D Imaging Specialist
February 2014 - March 2015
Mrc Harwell
Position
  • Postdoctoral Scientist Imaging
Education
February 2011 - March 2014
University of Birmingham
Field of study
  • Forensic Odontology, Biomedical Imaging
March 2006 - September 2010
Medical University of Vienna
Field of study
  • Human Medicine (Co-registration)
October 2005 - September 2010
University of Vienna
Field of study
  • Physical Anthropology: Osteology, passed with distinction

Publications

Publications (34)
Article
Full-text available
Human dental tissues consist of inorganic constituents (mainly crystallites of hydroxyapatite, HAp) and organic matrix. In addition, synthetic HAp powders are frequently used inmedical and chemical applications. Insights into the ultrastructural alterations of skeletal hard tissues exposed to thermal treatment are crucial for the estimation of temp...
Article
Micro- and ultrastructural analysis of burned skeletal remains is crucial for obtaining a reliable estimation of cremation temperature. Earlier studies mainly focused on heat-induced changes in bone tissue, while this study extends this research to human dental tissues using a novel quantitative analytical approach. Twelve tooth sections were burne...
Article
Understanding the degree and pattern of shrinkage undergone by bone when subjected to heating is crucial to accurately deduce a biological profile from incinerated remains. X‐ray microtomography (micro‐CT) enables a nondestructive insight into hard tissue structural changes, while allowing for an accurate documentation of volumetric and trabecular...
Data
This image is a close up macro shot composed of 66 individual images which have been focus-stacked to get the extended depth of field with the surface detail in focus. Nikon D800 with Nikkor 60mm f/2.8 Micro lens. Helicon Focus software.
Article
Peritoneal adhesions are fibrous tissues that tether organs to one another or to the peritoneal wall and are a major cause of postsurgical and infectious morbidity. The primary molecular chain of events leading to the initiation of adhesions has been elusive, chiefly due to the lack of an identifiable cell of origin. Using clonal analysis and linea...
Article
Full-text available
In the field of X-ray microcomputed tomography (μCT) there is a growing need to reduce acquisition times at high spatial resolution (approximate micrometers) to facilitate in vivo and high-throughput operations. The state of the art represented by synchrotron light sources is not practical for certain applications, and therefore the development of...
Article
Full-text available
Dietary restriction regimes extend lifespan in various animal models. Here we show that longevity in male C57BL/6J mice subjected to every-other-day feeding is associated with a delayed onset of neoplastic disease that naturally limits lifespan in these animals. We compare more than 200 phenotypes in over 20 tissues in aged animals fed with a lifel...
Article
Full-text available
The vertebrate Scube (Signal peptide, CUB and EGF-like domain-containing protein) family consists of three independent members Scube1-3, which encode secreted cell surface-associated membrane glycoproteins. Limited information about the general function of this gene family is available, and their roles during adulthood. Here, we present the first S...
Article
Full-text available
Animal models resembling human mutations are valuable tools to research the features of complex human craniofacial syndromes. This is the first report on a viable dominant mouse model carrying a non-synonymous sequence variation within the endothelin receptor type A gene (Ednra c.386A>T, p.Tyr129Phe) derived by an ENU mutagenesis program. The ident...
Article
Statement of significance: We studied the micron-scale residual stresses that exist within human teeth, between enamel (outer tooth shell, hardest substance in the human body) and dentine (soft bone-like vascularised tooth core). The dentine-enamel junction (DEJ) is an extremely interesting example of nature's design in terms of hierarchical struc...
Article
A laser-wakefield driven x-ray source is used for the radiography of human bone. The betatron motion of accelerated electrons generates x-rays which are hard (critical energy Ecrit <30 keV), have small source size (<3 μm) and high average brightness. The x-rays are generated from a helium gas cell which is near-instantly replenishable, and thus the...
Conference Paper
Extremely bright coherent femtosecond x-ray pulses are generated in compact laser-driven electron accelerators. Micro-tomography obtained with the Gemini laser indicates the usefulness of these sources in research and clinical applications.
Chapter
The most reliable and frequently applied method for identification of fire victims is comparative dental radiography. Forensic investigators are often confronted with fragmented and isolated dental remains, frequently removed from their anatomical position. Whilst the heat-induced dimensional and morphological changes in bone have been extensively...
Article
Full-text available
Terrorism is a complex issue and various researchers have identified numerous causes and conditions that generate or are capable of generating terrorism. In the last decade 36,495 lives have been lost besides injuring 27,985 people in Pakistan as a result of 3482 bomb blasts and 281 suicide attacks. The aim of this research was to establish the maj...
Conference Paper
Full-text available
The dentine-enamel junction (DEJ) is an important internal interface with strong and durable bonding between the hard outer layer (enamel) and the soft inner tooth core (dentine). This study investigated the microstructure of the DEJ by X-ray imaging method and synchrotron X-ray scattering techniques. Further high resolution elastic modulus mapping...
Article
Full-text available
Context: Only a few methods have been published dealing with the visualization of heat-induced cracks inside bones and teeth. Aims: As a novel approach this study used nondestructive X-ray microtomography (micro-CT) for volume analysis of heat-induced cracks to observe the reaction of human molars to various levels of thermal stress. Materials...
Chapter
Teeth subjected to high temperatures following natural disasters, airplane crashes or house fires can sometimes be the only remains available for forensic identification and are also frequently found as part of cultural cremations in an archaeological context. It is therefore important to characterise any tissue changes that take place in such high...
Thesis
Full-text available
In forensic investigations involving severely burned human remains, dental analysis stands alone as other means of identification are often destroyed. Therefore, the aim of the present work was to investigate the influence of duration of heat exposure and heating regimes regarding the macroscopic, compositional, structural and crystalline alteratio...
Article
Human teeth consist of enamel, dentine and cementum, hierarchical mineralised tissues with a two-level composite structure. The understanding of the mechanical behaviour of dentine and enamel in terms of their micro- and nano-scale structure has been somewhat limited. Here we present an overview of our recent work aimed at improving the understandi...
Conference Paper
Full-text available
After attending this presentation, attendees will have gained an understanding of the potential of micro-computed tomography for the advancement of forensic anthropological research as a non-destructive means of measuring changes in the bones’ micro-architecture, volume and stereology. This presentation will impact the forensic community by demon...
Article
Human teeth are hierarchical mineralized structures consisting of collagen fibres decorated with hydroxyapatite (HAp) crystallites at the nano-scale. Teeth are composed of two major calcified tissues: harder outer enamel layer and softer dentine core. These tissues are joined by an interface known as the dentine-enamel junction (DEJ). The knowledge...
Article
The knowledge of the mechanical properties of dental materials related to their hierarchical structure is essential for understanding and predicting the effect of microstructural alterations on the performance of dental tissues in the context of forensic and archaeological investigation as well as laser irradiation treatment of caries. So far, few...
Article
Human enamel is a typical hierarchical mineralized tissue with a two-level composite structure. To date, few studies have focused on how the mechanical behaviour of this tissue is affected by both the rod orientation at micro-scale and the preferred orientation of mineral crystallites at the nano-scale. In this study, Wide Angle X-ray Scattering (W...
Article
Full-text available
Human enamel is a hierarchical mineralized tissue with a two-level composite structure. Few studies have focused on the structure-mechanical property relationship and its link to the multi-scale architecture of human enamel, whereby the response to mechanical loading is affected not only by the rod distribution at micro-scale, but also strongly inf...
Article
Full-text available
Teeth subjected to high temperatures following natural disasters, airplane crashes or house fires can sometimes be the only remains available for forensic identification. The information on macro- and microscopic heat-induced changes of teeth can therefore provide relevant information that can facilitate the identification process. An important mac...
Conference Paper
Objective: The structural and functional characterization of the nanostructures in natural dental tissues allows the morphological and structural composition to be determined which provides essential information for the design of future dental treatments and restoration materials. Synchrotron-based small-angle X-ray scattering (SAXS) enables the hi...
Conference Paper
Mineral trioxide aggregate (MTA) has been shown to induce profound wound healing responses to the injured dental pulp following its application, however the biological mechanism of action is yet to be fully explained. Solubilisation of ionic components from these materials by local tissue fluids may lead to the creation of an environment in which t...
Article
Human dentine is a hierarchical mineralized tissue with a two-level composite structure, with tubules being the prominent structural feature at micro-level, and collagen fibres decorated with hydroxyapatite (HAp) crystallite platelets at nano-scale. Few studies have focused on this two-level structure of human dentine, where the response to mechani...
Thesis
Full-text available
By now, only a few methods have been published dealing with the visualization of heat-induced cracks and fissures inside bones and teeth. During the last few decades the focus of interest shifted from the macroscopic to the microscopic level of heat-induced changes. In this diploma thesis, the method of non-invasive x-ray micro tomography (micro-CT...

Questions

Questions (9)
Question
As there are multiple tissue clearing techniques around at the moment, BABB is seemingly the most commonly used for standard optical projection tomography imaging. Have you tried any alternative like e.g. SeeDB, 3DISCO, CLARITY, ClearT/T2? Also, how well have these techniques worked with (multiple) antibody labeling?
Question
I just try to put together some lecture notes about possible artifacts that can occur during micro-CT data acquisition and somehow I haven't yet come across an in-depth review covering multiple types of micro-CT artifact (ring, misalignment, partial-volume, noise, beam hardening etc.). Do you have any recommendation for a paper/book chapter/online-lecture?