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Michael E Kjelland

Michael E Kjelland
Conservation, Genetics & Biotech, LLC · R&D

M.S., Ph.D.
https://www.youtube.com/watch?v=bh9dhT8Cam4

About

164
Publications
21,604
Reads
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745
Citations
Introduction
Additional affiliations
October 2012 - August 2020
Conservation, Genetics & Biotech, LLC
Position
  • CEO
Description
  • www.michaelkjelland.com Video clip using RFID wireless Inteli-straw technology (Inteli-straw, LLC) with livestock here: https://www.youtube.com/watch?v=n0NR7PxVBO0 See a collaboration with Hoofstock Genetics, LLC and Inteli-straw, LLC and our first Assisted Reproductive Technologies (AI & ET) 360 VR video here: https://www.youtube.com/watch?v=gyAraqLTIcQ
June 2011 - present
Fossil Excavators, LLC
Position
  • CEO
Description
  • www.fossilexcavators.com
Education
June 2001 - December 2008
Texas A&M University
Field of study
  • Wildlife & Fisheries Sciences, Ecological Modeling, and Reproductive Sciences

Publications

Publications (164)
Article
Full-text available
SexedULTRA-4M™ is made using an improved method of sex-sorting sperm in a less damaging environment for better retaining sperm integrity throughout the sorting process. The objective of this research was to compare conventional (CONV) and SexedULTRA-4M™ (ULTRA-4M) semen for bovine IVP using four Angus bulls. Matured slaughterhouse oocytes (n = 4000...
Article
The transfer of cryopreserved embryos has great potential to disseminate valuable genetics in cattle; however, a suitable protocol for cryopreservation of in vitro-produced embryos (IVP) for direct transfer is still being improved. The combination of IVP and cryopreservation can be further enhanced when associated with the new sexed semen technolog...
Article
Sexed semen technology has been modernised and is now known as ULTRA™ (STgenetics). In laboratory trials with in vitro-produced (IVP) bovine embryos, there was no difference between conventional semen (CONV) and X chromosome-bearing SexedULTRA-4M semen (ULTRA-4M) in blastocyst production. However, the scientific literature lacks information about I...
Article
In vitro embryo production (IVP) is an important tool for genetic improvement in small ruminants. For the success of IVP, semen quality is one of the most important aspects to consider. The differences between ejaculates from the same animal (especially during different seasons) is well documented in domestic sheep (Ovis aries). The objective of th...
Poster
Full-text available
Ferulic acid (4-hydroxy-3-methoxy cinnamic acid) is a phenolic compound and can be extracted in high concentrations from the cell wall of wheat bran. The objective of this study was as follows: 1) to successfully culture pancreatic cancer cells, 2) determine whether ferulic acid addition to cells causes cell death and deterioration, 3) compare effe...
Poster
Full-text available
Arabinoxylan (AX) has beneficial impacts on many physiology processes, including the ability to interact with gut bacteria and result in a prebiotic manner. The enzymatic degradation of arabinoxylan along with hydrolysis produces arabinoxylan oligosaccharides (AXOS) results in health benefits including immunomodulatory activity. The arabinoxylan wa...
Article
Domestic cat in vitro embryo production (IVEP) begins with IVM of oocytes to produce mature oocytes; that is, MII. The domestic cat (Felis catus) has been used as a model to carry out assisted reproductive technology (ART) research for application in wild feline species that may be threatened or endangered. The objective of this research was to eva...
Article
Sperm selection methods are routinely applied to prepare semen for IVF in animal species. These procedures are used to improve sperm quality characteristics as well as to remove other background material and debris. Percoll gradient is widely used in animal IVF laboratories. Different Percoll gradient concentrations and volumes can be used to impro...
Article
SexedULTRA 4M™ (Select Sires) is made using an improved method of sex-sorting sperm in a less damaging environment for retaining sperm integrity through the sorting process. The combination of in vitro embryo production (IVP) and sexed semen technologies has been successful for intensity selection in the cattle industry. The objective of this resea...
Article
In vitro embryo production (IVP) is an important tool for genetic improvement in small ruminants. Semen quality is one of the most important aspects to consider for the success of this assisted reproductive technique. With ovine IVP, it is a common practice to use fresh semen for IVF, but this could be a problem because the differences between ejac...
Article
Colour-Doppler ultrasonography (CDU) has been applied for a more detailed examination of the ovary and uterus, mainly local blood flow in ovarian follicles and the corpus luteum (CL). The main use of CDU is for the diagnosis of early pregnancy or the selection of recipients for embryo transfer in cattle; however, this tool can also be used to selec...
Poster
Full-text available
SexedULTRA 4M™ (Select Sires) is made using an improved method of sex-sorting sperm in a less damaging environment for retaining sperm integrity through the sorting process. The combination of in vitro embryo production (IVP) and sexed semen technologies has been successful for intensity selection in the cattle industry. The objective of this resea...
Poster
Full-text available
Sperm selection methods are routinely applied to prepare semen for IVF in animal species. These procedures are used to improve sperm quality characteristics as well as to remove other background material and debris. Percoll gradient is widely used in animal IVF laboratories. Different Percoll gradient concentrations and volumes can be used to impro...
Poster
Full-text available
In vitro embryo production (IVP) is an important tool for genetic improvement in small ruminants. Semen quality is one of the most important aspects to consider for the success of this assisted reproductive technique. With ovine IVP, it is a common practice to use fresh semen for IVF, but this could be a problem because the differences between ejac...
Poster
Full-text available
Colour-Doppler ultrasonography (CDU) has been applied for a more detailed examination of the ovary and uterus, mainly local blood flow in ovarian follicles and the corpus luteum (CL). The main use of CDU is for the diagnosis of early pregnancy or the selection of recipients for embryo transfer in cattle; however, this tool can also be used to selec...
Poster
Full-text available
Domestic cat in vitro embryo production (IVEP) begins with IVM of oocytes to produce mature oocytes; that is, MII. The domestic cat (Felis catus) has been used as a model to carry out assisted reproductive technology (ART) research for application in wild feline species that may be threatened or endangered. The objective of this research was to eva...
Article
The Pterostilbene (PT) molecule is a phytoalexin with a reducing‐effect on reactive oxygen species (ROS) and with a capacity to block lipogenesis. However, the potential reducing effects of PT on equatorial lipid accumulation and ROS have not yet been elucidated for in vitro‐derived bovine embryos. The present study evaluated the effects of concent...
Technical Report
Full-text available
This report assesses potential impacts to aquatic resources resulting from proposed navigation channel expansion activities within Mobile Bay, Ala-bama. This work was conducted for the U.S. Army Corps of Engineers (USACE) Mobile District, to support development of a supplemental Envi-ronmental Impact Statement. Changes in water quality and hydrodyn...
Article
Contents Cryopreservation of in vitro‐derived bovine embryos is a crucial step for the widespread reproduction and conservation of valuable high‐merit animals. Given the current popularity of bovine in vitro embryo production (IVP), there is a demand for a highly efficient ultra‐low temperature storage method in order to maximize donor ovum pickup...
Poster
Full-text available
In vitro embryo production (IVP) can increase the reproductive potential and genetic quality of cattle, as well as other species. This powerful assisted reproduction tool can be used to produce embryos from prepubertal calves, reducing the generation interval. A recent sexed semen technology known as SexedULTRA 4M® (STgenetics®), completely modifie...
Poster
Full-text available
Isolation of germinal vesicle (GV) or metaphase I (MI) oocytes from large antral follicles in hyperstimulated mice is a common technique for the retrieval of immature oocytes from ovaries. Mice are usually primed with pregnant mare serum gonadotropin (PMSG), and large antral follicles are punctured under a microscope to isolate GV or MI oocytes. Is...
Poster
Full-text available
The first commercial production of sexed semen was at the Cogent company in the United Kingdom. Since then millions of offspring have been born using sexed semen produced by flow cytometry. Currently, the technology of sexed semen has been modernized to what is now known as SexedULTRA ® (STgenetics ®), completely modifying the technique, the media...
Poster
Full-text available
White-tailed Deer (WTD) in vitro embryo production (IVP) is not well documented, in part due to the seasonality of wildlife species and available hunting permits. It is noteworthy that more than half of the 45 species (or subspecies) in the family Cervidae are threatened or endangered with extinction. Assisted reproductive technologies (ARTs), incl...
Poster
Full-text available
The aim of this study was to compare OPU yields, developmental competence of oocyte cumulus complexes (COCs) and pregnancy rates from Angus donors in a commercial IVP setting. Synchronization, FSH stimulation and 48 h coasting prior to ovum pick up in Angus cows increased the number of collected viable oocytes and embryo development rates. More tra...
Article
Aims The present study aims to define maturation, yield, health, and ease of collection of murine immature oocytes recovered using the conventional method or from mice treated with cilostazol. Main methods The conventional method included the superovulation of mice and the recovery of germinal vesicle (GV) or metaphase (MI) oocytes from preovulato...
Article
White-tailed deer (WTD) in vitro embryo production is not well documented, due to the seasonality of the wildlife species and available hunting permits. The objective of the present study was to conduct IVM and IVF using WTD oocytes vitrified with trehalose (TH) or sucrose (SC). A total of 121 immature oocytes were obtained from ovaries (n = 18) us...
Article
In vitro embryo production (IVP) has become a reliable alternative for genetic improvement in beef and dairy herds. Maximizing embryo yield and pregnancy per donor are key factors. The aim of this study was to compare ovum pickup (OPU) yields, developmental competence of cumulus-oocyte complexes (COCs), and pregnancy rates from Angus donors in a co...
Article
The first commercial production of sexed semen was at the Cogent company in the United Kingdom. Since then millions of offspring have been born using sexed semen produced by flow cytometry. Sexed semen technology has recently been modernized to what is now known as ULTRA (ST Genetics), completely modifying the technique, medium, and sperm concentra...
Article
In vitro embryo production (IVP) can increase the reproductive potential and genetic quality of cattle, as well as other species. This powerful assisted reproduction tool can be used to produce embryos from prepubertal calves, reducing the generation interval. A recent sexed semen technology known as ULTRA (ST Genetics), completely modified the tec...
Article
Administration of cilostazol in mice and swine has resulted in the ovulation of immature oocytes at the germinal vesicle (GV) or metaphase I (MI) stages. The present study aimed to define oocyte synchronised maturation, yield, health, and ease of collection from mice treated with cilostazol. The conventional method included mice primed with pregnan...
Article
Full-text available
The aim of the present research was to simulate the Salton Sea elevation, volume, and total dissolved solids (TDS) to assess 34 different scenarios through the year 2024 in order to better evaluate the effects of potential water management scenarios. Parameterization of an existing Salton Sea simulation model, i.e., Salton Sea Stochastic Simulation...
Article
Full-text available
In vitro production ( IVP ) of embryos and associated technologies in cattle have shown significant progress in recent years, in part driven by a better understanding of the full potential of these tools by end users. The combination of IVP with sexed semen ( SS ) and genomic selection ( GS ) is being successfully and widely used in North America,...
Article
Full-text available
Nearly all animal species have utilized photoperiod to cue seasonal behaviors and life history traits. We investigated photoperiod responses in keystone species, Daphnia magna, to identify molecular processes underlying ecologically‐important behaviors and traits using functional transcriptomics analyses. Daphnia magna were photoperiod‐entrained im...
Article
Sperm cryopreservation facilitates the storage and transport of germplasm for its use in artificial insemination (AI) and other advanced reproductive technologies. The cryopreservation process can damage sperm and compromise functionality. Several cryobiological studies have found that the physical and biological factors that affect sperm survival...
Article
Full-text available
Macro-imagineered computer model of Salton Sea alternative futures under climate uncertainty and water transfer considerations. February 2019.
Patent
Full-text available
A container system for cryogenically and non-cryogenically storing and identifying biological material stored therein wherein the container system includes semen straws, vitrification straws, and canes for holding such straws, the present improvement including positioning and locating a radio frequency identification (RFID) tag on or within semen s...
Data
This study used Pterostilbene to optimize the in vitro embryo culture system. Pterostilbene, which has the capacity to block the lipogenesis in other cell lineages, was used in the embryo culture media to test whether or not supplementing embryos with Pterostilbene decreases intracytoplasmatic lipid accumulation.
Article
The scientific literature lacks information regarding the incubation conditions and hatching success of the resplendent quetzal (Pharomachrus mocinno mocinno). Jesús Estudillo López, founder of the El Nido aviary, was the first to succeed with quetzal reproduction in captivity. Quetzals do not typically reproduce in captivity and the aim of this st...
Article
Multiple ovulation and embryo transfer (MOET) is a biotechnology widely used for genetic improvement in cattle; however, there is great variability in donor response. Differences between management, genotype, environmental conditions, among other factors, can affect MOET results. The traditional protocols consist of 8 twice-daily FSH applications i...
Article
Improving in vitro production efficiency involves the development of effective oocyte in vitro maturation conditions. Although >80% of cumulus-oocyte complexes (COC) undergo nuclear maturation, only approximately 30 to 35% of immature bovine COC develop to the blastocyst stage. Also, animal-sourced FSH is typically used in IVF, so an effective alte...
Article
Assisted reproductive technologies (ART) continue to develop rapidly, allowing for the development of techniques to increase reproductive efficiency and contribute to the genetic improvement of cattle. Some of these techniques include in vitro production (IVP) of embryos and embryo transfer. These modern ART can help produce offspring with highly d...
Article
Reproductive biotechnology has continued to evolve rapidly, allowing the development of techniques to increase reproductive efficiency and contribute to the genetic improvement of cattle. Some of these techniques include the in vitro maturation and IVF of oocytes, sperm sexing and cloning. These modern assisted reproductive techniques can help prod...
Article
Various permeating cryoprotectants, such as glycerol and ethylene glycol, have been used in the cryopreservation of embryos to help maintain cellular viability during indefinite and prolonged periods of storage in liquid nitrogen. The objective of this study was to compare the efficiency of glycerol (G) and ethylene glycol (EG) after storage in liq...
Poster
Full-text available
Results: A total of 27 quetzal eggs [mean weight (g) = 17.3, s.d. = 1.2] were incubated over 3 years, of which 10 (~37%) hatched, with 3 (30%) fledging and surviving to adulthood (Figure 1c, d). Six of the eggs (~22%) suffered embryonic mortality during incubation, whereas 11 eggs were infertile (~41%). Hatchability over the study period was 62.5%...
Poster
Full-text available
Improving in vitro production efficiency involves the development of effective oocyte in vitro maturation conditions. Although >80% of cumulus-oocyte complexes (COC) undergo nuclear maturation, only approximately 30 to 35% of immature bovine COC develop to the blastocyst stage. Also, animal-sourced FSH is typically used in IVF, so an effective alte...
Poster
Full-text available
Multiple Ovulation and Embryo Transfer (MOET) is a biotechnology widely used for genetic improvement in cattle; however, there is great variability in donor response. Differences between management, genotype, environmental conditions, among other factors, can affect MOET results. The traditional protocols consist of 8 twice-daily FSH applications i...
Poster
Full-text available
Assisted reproductive technologies (ART) continue to develop rapidly, allowing for the development of techniques to increase reproductive efficiency and contribute to the genetic improvement of cattle. Some of these techniques include in vitro production (IVP) of embryos and embryo transfer. These modern ART can help produce offspring with highly d...
Poster
Full-text available
Reproductive biotechnology has continued to evolve rapidly, allowing the development of techniques to increase reproductive efficiency and contribute to the genetic improvement of cattle. Some of these techniques include the in vitro maturation and IVF of oocytes, sperm sexing and cloning. These modern assisted reproductive techniques can help prod...
Poster
Full-text available
Various permeating cryoprotectants like Glycerol and Ethylene Glycol have been used in the cryopreservation of embryos to help maintain cellular viability during indefinite and prolonged periods of storage in liquid nitrogen. The objective of this research was to compare the efficiency of Glycerol (G) and Ethylene Glycol (EG) after storage in liqui...
Poster
Full-text available
Industrial polluters play a major role in the effects of ecotoxicity translated to apex predators. Bioaccumulation of harmful metals up the food chain is an alarming human-health concern, particularly in at-risk populations such as young children and women of child-bearing age. Alligator meat has been touted as a healthy protein alternative to beef...
Article
Full-text available
Transvaginal ultrasound needle-guided ovum pick-up (OPU) and IVP represent powerful tools to multiply selected females in a short period of time. Maximizing the quantity and quality of retrieved oocytes is key for obtaining the best embryo development and implantation rates. The aim of this study was to compare OPU yields and developmental competen...
Article
Efecto del pH de cinco soluciones extensoras sobre la movilidad espermática en trucha arcoíris (Oncorhynchus mykiss) Effect of pH on rainbow trout (Oncorhynchus mykiss) sperm motility using five extender solutions Recibido: 29 de mayo de 2017. Aceptado: 26 de junio de 2018. Peralta-Martínez M. de los Á., S. Romo García, M. E. Kjelland y H. González...
Patent
Full-text available
The present invention is a cryogenic storage receptacle for RFID data made up of a cryogenic container having a delivery end and a tag end partially closed by a pressure equalization cap. A RFID tag is located adjacent to the tag end. Partial closure of the tag end by pressure equalization cap allows a plunger arm to push the plunger and expel the...
Article
To more accurately predict recruitment of zebra mussels (Dreissena polymorpha) in waterbodies downstream of a source population, factors that control the successful transport of zebra mussel larvae need to be quantified. Turbulence has been identified in previous studies as a likely factor in larval morality in rivers and streams where turbulent en...
Article
It is known that Zinc (Zn) is important in several aspects of reproduction, including fertility and the production of sexual hormones. The aim of the present study was to evaluate different amounts and types of Zn on ejaculate collection time and training behaviour of young boars. For this purpose, non-castrated domestic boars (York × Landrace, Sus...
Article
Zinc (Zn) is essential for the development and activity of sperm, although its cytotoxic effect on sperm has been little studied. This study evaluated the effect of organic Zn; that is, Zn-methionate (Zn-Met), on the DNA fragmentation of boar sperm. Domestic boars (York × Landrace, Sus scrofa domesticus; n = 15) were randomly allocated into 3 level...
Article
Rainbow trout (Oncorhynchus mykiss) sperm extender protocols can differ considerably with regards to composition of the extenders and handling. The objective of this study was to determine the effect of pH for 5 extenders on rainbow trout sperm motility and activation during storage at 5°C. Two-year-old sexually mature rainbow trout males (n = 46)...
Article
Inteli-Straw (I-S) devices equipped with radiofrequency identification (RFID) technology were developed for gamete and embryo packaging, storage, and information retrieval to benefit the assisted reproduction industry. The aim of this study was to develop and test software for use with I-S technology. Two types of I-S were used, those with 125 and...
Article
Full-text available
Although considered a species of least concern by the International Union for Conservation of Nature red list, the Desert Bighorn sheep (Ovis canadensis nelsoni) is listed in Appendix II of CITES (Convention on International Trade in Endangered Species of Wild Fauna and Flora) in Mexico, due to population size and the lack of protected areas. Postm...
Poster
Rainbow trout (Oncorhynchus mykiss) sperm extender protocols can differ considerably with regards to composition of the extenders and handling. The objective of this study was to determine the effect of pH for 5 extenders on rainbow trout sperm motility and activation during storage at 5°C. Two-year-old sexually mature rainbow trout males (n = 46)...
Poster
Full-text available
Zinc (Zn) is essential for the development and activity of sperm, although its cytotoxic effect on sperm has been little studied. This study evaluated the effect of organic Zn; that is, Zn-methionate (Zn-Met), on the DNA fragmentation of boar sperm. Domestic boars (York × Landrace, Sus scrofa domesticus; n = 15) were randomly allocated into 3 level...
Poster
Full-text available
It is known that Zinc (Zn) is important in several aspects of reproduction, including fertility and the production of sexual hormones. The aim of the present study was to evaluate different amounts and types of Zn on ejaculate collection time and training behaviour of young boars. For this purpose, non-castrated domestic boars (York × Landrace, Sus...
Poster
Full-text available
Inteli-Straw (I-S) devices equipped with radiofrequency identification (RFID) technology were developed for gamete and embryo packaging, storage, and information retrieval to benefit the assisted reproduction industry. The aim of this study was to develop and test software for use with I-S technology. Two types of I-S were used, those with 125 and...
Poster
Full-text available
Although considered a species of least concern by the International Union for Conservation of Nature red list, the Desert Bighorn sheep (Ovis canadensis nelsoni) is listed in Appendix II of CITES (Convention on International Trade in Endangered Species of Wild Fauna and Flora) in Mexico, due to population size and the lack of protected areas. Postm...
Article
Full-text available
Purpose: Anti-sperm antibodies (ASA) in men impair not only sperm motility but also fertilization and conception. However, utilization of corticosteroids to suppress ASA has shown variable pregnancy outcomes. This controversy is also extended to include the usefulness of conventional in vitro fertilization (IVF) and intracytoplasmic sperm injectio...
Conference Paper
Un desafío para la mayoría de los centros de investigación involucra al medio de cultivo embrionario, ya que juega un papel importante en la calidad embrionaria. Los sistemas de Producción in vitro (PIV), han tratado de simular el ambiente fisiológico in vivo. Cuando se llegan a hacer comparaciones entre los embriones que han sido producidos in vit...
Article
The present research demonstrates a novel example of merging agent-based, spatially-explicit simulation modelling results, while specifically addressing different model scenarios, with 3-dimensional (3D) printing technology for the production of tangible 3D data output. Based on our knowledge, this study provides the first demonstration of using sp...
Article
Contents Straws of sex‐sorted sperm are usually packaged at a low concentration (e.g., ~2.1 × 10⁶ sperm/ml) and cost significantly more than unsorted conventional semen from the same sire. In order to maximize the efficiency of using sex‐sorted sperm under in vitro fertilization conditions, the selection of an appropriate sperm separation technique...
Poster
Full-text available
Rainbow trout (Oncorhynchus mykiss) sperm extender protocols can differ considerably with regard to composition of the extenders and handling. The objective of this study was to determine the effect of pH for 5 extenders on Rainbow trout sperm motility and activation during storage at 5°C. Sperm extenders (i.e., 306, 512, Mounib’s, Erdahl & Graham,...
Poster
Full-text available
In order to understand the impacts of managing oyster reefs for environmental and economic concerns, we used the integrated Chesapeake Bay Oyster Population Model (CBOPM) to simulate eastern oyster (Crassostrea virginica) dynamics under seven different management scenarios that included combinations of reefs dedicated as reserves and reefs subject...
Article
In vitro production of germline chimeras and avian cloning may utilise the transfer of avian embryos from their original eggshell to a surrogate eggshell for culture during incubation. Such embryo transfer is valuable for avian cloning as the only alternative would be to transfer the cloned avian embryos into the infundibulum of recipient birds. Gi...
Article
The Colorado River Quantification Settlement Agreement (QSA) of 2003 gives urgency for studying the environmental consequences of the cessation of mitigation water transfers to the Salton Sea. The Salton Sea Stochastic Simulation Model (S⁴M) is a spatially-driven, stochastic, simulation model representing water flow, i.e., water volume and quantity...