Michael Hadjiargyrou

Michael Hadjiargyrou
New York Institute of Technology | NYIT · Department of Biological & Chemical Sciences

PhD

About

218
Publications
21,821
Reads
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5,611
Citations
Introduction
The overall goal of my research is to implement innovative approaches for engineering new musculoskeletal tissue utilizing knowledge derived from basic molecular and cellular biology and biomaterials. More specifically, we are actively involved in understanding the molecular mechanisms that underlie musculoskeletal development and regeneration. My laboratory is also involved in tissue engineering research by focusing on the fabrication and characterization of biomimetic scaffolds.
Additional affiliations
September 1998 - August 2012
Stony Brook University
Position
  • Professor (Associate)
March 1996 - August 1998
Stony Brook University
Position
  • Professor (Assistant)
February 1992 - February 1996
California Institute of Technology
Position
  • Post-doctoral Fellowship
Education
January 1988 - January 1992
City University of New York - Hunter College
Field of study
  • Molecular/Cell Biology
September 1986 - December 1987
September 1982 - May 1986

Publications

Publications (218)
Article
The development of effective strategies for gene delivery is a critical goal in DNA-based tissue engineering. Previously, our laboratory utilized the process of electrospinning to fabricate plasmid DNA-based polymeric scaffolds. Although the release of DNA was robust, the in vitro transfection efficiency was low. In order to optimize these results,...
Article
The Mustn1 gene encodes a small nuclear protein (~9.6 kDa) that does not belong to any known family. Its genomic organization consists of three exons interspersed by two introns and it is highly homologous across vertebrate species. Promoter analyses revealed that its expression is regulated by the AP family of transcription factors, especially c-F...
Article
The repair of a fractured bone is critical to the well‐being of humans. Failure of the repair process to proceed normally can lead to complicated fractures, exemplified by either a delay in union or a complete nonunion. Both of these conditions lead to pain, the possibility of additional surgery, and impairment life quality. Additionally, work prod...
Article
Full-text available
Antibiotic resistance represents a threat to human health. It has been suggested that by 2050, antibiotic-resistant infections could cause ten million deaths each year. In orthopaedics, many patients undergoing surgery suffer from complications resulting from implant-associated infection. In these circumstances secondary surgery is usually required...
Article
Methylphenidate (MP) is a psychostimulant chronically prescribed for the treatment of attention deficit hyperactivity disorder (ADHD). Additionally, MP users may take breaks from using the medication during “drug holidays” which may include short‐term or long‐term breaks from medication. The present study utilized fluorodeoxyglucose (FDG) positron...
Article
Methylphenidate (MP) is extensively prescribed for attention deficit hyperactivity disorder (ADHD). While MP is effective in ameliorating symptoms of ADHD, MP is also used illicitly among healthy subjects without ADHD for cognitive-enhancing purposes. The deleterious consequences associated with long-term MP use as well as its cessation on brain ac...
Article
Full-text available
The utilization of the mRNA-based Pfizer-BioNTech and Moderna coronavirus disease 2019 (COVID-19)vaccines represents the culmination of many years of nonviral nucleic acid delivery, but more importantly, they signify a massive clinical scientific success. Scientists working in the area of nucleic acid delivery using lipid nanoparticles will undoubt...
Article
Introduction Methylphenidate (MP) is a widely used psychostimulant prescribed for Attention Deficit Hyperactivity Disorder, and is also used illicitly by healthy individuals. Chronic exposure to MP has been shown to affect physiology, behavior, and neurochemistry. Methods The present study examined its effect on the endocannabinoid system. Adolesc...
Article
During the past several years, pre-clinical experiments have established that microRNAs (miRNAs), small non-coding RNAs, serve as key regulatory molecules of fracture healing. Their easy modulation with agonists and antagonists make them highly desirable targets for future therapeutic strategies, especially for pathophysiologic fractures that eithe...
Article
Aim Examine the effects of chronic oral Methylphenidate (MP) treatment on the N-Methyl-D-aspartic acid (NMDA) glutamate receptor binding in the rat brain using a previously established drinking paradigm that has been shown to deliver MP with similar pharmacokinetic profile as observed clinically. Main methods Briefly, rats were divided into three...
Poster
Mustn1 is a novel pan-musculoskeletal marker discovered during an expression screen for genes upregulated during bone regeneration. While it does not belong to any known gene family, it has been found to be primarily expressed in bone, cartilage, skeletal muscle, and tendon. Mustn1 gene expression has been found in a wide range of pathologies and s...
Article
Methylphenidate (MP) is a commonly prescribed psychostimulant to individuals with Attention Deficit Hyperactivity Disorder, and is often used illicitly among healthy individuals with intermittent breaks to coincide with breaks from school. This study examined how intermittent abstinence periods impact the physiological and behavioral effects of chr...
Conference Paper
Purpose: Dry eye is a common condition treated primarily by topical lubricants, immunomodulation, and a variety of punctal and canalicular plugs (occluders). Biofilm formation has been reported in the literature as an ongoing problem with the clinical use of occluders. In order to explore the role of biofilm formation on occluders, we tested two st...
Article
Full-text available
Dry eye is a common condition that is treated primarily by topical lubricants, immunomodulation, and a variety of punctal and canalicular plugs (occluders). Biofilm formation has been reported as an ongoing problem with the clinical use of occluders. In order to explore the role of biofilm formation on occluders, we tested the bacteria strain, Stap...
Article
Full-text available
Methylphenidate (MP) is a widely prescribed psychostimulant used to treat attention deficit hyperactivity disorder. Previously, we established a drinking paradigm to deliver MP to rats at doses that result in pharmacokinetic profiles similar to treated patients. In the present study, adolescent male rats were assigned to one of three groups: contro...
Article
Methylphenidate (MP) is a commonly prescribed psychostimulant for Attention Deficit Hyperactivity Disorder (ADHD). We recently reported behavioral and developmental effects of chronic MP use in healthy rats. The current study investigated how interrupting chronic MP treatment with weekend abstinence altered the behavioral and physiological conseque...
Article
Chronic oral methylphenidate (MP) exposure in rats is associated with numerous developmental and behavioral consequences. The present study investigated the persistence of the effects of chronic oral MP exposure after abstinence from MP use. Male and female rats were exposed to daily orally self-administered water, low dose MP (LD), or high dose (H...
Article
Ketamine is one of several clinically important drugs whose therapeutic efficacy is due in part to their ability to act upon ion channels prevalent in nearly all biological systems. In studying eukaryotic and prokaryotic organisms in vitro, we show that ketamine short-circuits the growth and spatial expansion of three microorganisms, Stachybotrys c...
Article
Full-text available
Methylphenidate (MP) is the most prescribed psychostimulant for ADHD patients, with clinically demonstrated detrimental effects on bone quality, potentially leading to early onset osteoporosis and higher fracture risk. The underlying mechanism for the effects of MP on bone remains elusive. This study demonstrates that sex- and dose-dependent effect...
Article
Amphiphilic cationic poly(ethylene oxide)-S(polylysine)-poly(d,l-lactide) (mPEO-S(CKn)-PLA) tri-arm star copolymers were synthesized by a combination of ring opening polymerization (ROP) and a thiol–disulfide exchange. The mPEO-S(CKn)-PLA copolymers were found to be non-cytotoxic and could effectively condense GFP plasmid DNA into nanometer-sized c...
Article
Full-text available
Background: Increasing production of nanomaterials requires fast and proper assessment of its potential toxicity. Therefore, there is a need to develop new assays that can be performed in vitro, be cost effective, and allow faster screening of engineered nanomaterials (ENMs). Results: Herein, we report that titanium dioxide (TiO2) nanoparticles (NP...
Article
Full-text available
Previously, we created an 8-h limited-access dual bottle drinking paradigm to deliver methylphenidate (MP) to rats at two dosages that result in a pharmacokinetic profile similar to patients treated for attention deficit hyperactivity disorder. Chronic treatment resulted in altered behavior, with some effects persisting beyond treatment. In the cur...
Article
Full-text available
Methylphenidate (MP) is a psychostimulant prescribed for Attention Deficit Hyperactivity Disorder. Previously, we developed a dual bottle 8-h-limited-access-drinking-paradigm for oral MP treatment of rats that mimics the pharmacokinetic profile of treated patients. This study assessed sex differences in response to this treatment. Male and female S...
Article
Full-text available
Scientific misconduct is a serious violation of society’s trust in scientists to self-regulate and police their profession. In the past decade, we have seen a disturbing trend of a steady increase in both, the number of allegations and findings of data fabrication, falsification and plagiarism, the hallmarks of scientific misconduct. A number of pu...
Article
Osteoporosis is the most common metabolic bone disorder and its management represents a tremendous public health encumbrance. While several classes of therapeutics have been approved to treat this disease, all are associated with significant adverse effects. An algorithm was developed and utilized to discover potential bioactive peptides, which led...
Article
Most animal studies using methylphenidate (MP) do not administer it the same way it is administered clinically (orally), but rather by injection, resulting in an altered pharmacokinetic profile (i.e. quicker and higher peak concentrations). Here, we evaluated several oral-dosing regimens in rats, including dual-dose drinking, to mimic the clinical...
Article
The complexity of fracture repair makes it an ideal process for studying the interplay between the molecular, cellular, tissue and organ level events involved in tissue regeneration. Additionally, as fracture repair recapitulates many of the processes that occur during embryonic development, investigations of fracture repair provide insights regard...
Article
To celebrate the success of the Journal of Controlled Release and the research covered in the journal, here we highlight some of the most cited research articles in the history of the journal. Based on the literature search in Google Scholar in July 2013, we identified ~30 research articles that have received most number of citations. Authors of th...
Article
To celebrate the success of the Journal of Controlled Release and the research covered in the journal, here we highlight some of the most cited research articles in the history of the journal. Based on the literature search in Google Scholar in July 2013, we identified ~30 research articles that have received most number of citations. Authors of th...
Article
Full-text available
Osseointegration of bone implants is a vital part of the recovery process. Numerous studies have shown that micropatterned geometries can promote cell-substrate associations and strengthen the bond between tissue and the implanted material. As demonstrated previously, exogenous zinc levels can influence the responsiveness of pre-osteoblasts to micr...
Article
In this study, the osteogenic differentiation of murine pre-osteoblasts cultured on patterned silicon was investigated. Specifically, micropatterns were fabricated in <100> orientation silicon wafers to create linear gratings with a width of either 2 µm (2 µm depth, 10 µm pitch) or 20 µm (2 µm depth, 30 µm pitch). Two subclones (4, 24) of the MC3T3...
Article
Initial cell-surface interactions are guided by the material properties of substrate topography. To examine if these interactions are also modulated by the presence of zinc, we seeded murine pre-osteoblasts (MC3T3-E1, subclone 4) on micropatterned polydimethylsiloxane (PDMS) containing wide (20 µm width, 30 µm pitch, 2 µm height) or narrow (2 µm wi...
Article
Full-text available
Growing evidence shows a close association of transposable elements (TE) with non-coding RNAs (ncRNA), and a significant number of small ncRNAs originate from TEs. Further, ncRNAs linked with TE sequences participate in a wide-range of regulatory functions. Alu elements in particular are critical players in gene regulation and molecular pathways. A...
Article
Mustn1 has been implicated in myofusion as well as skeletal muscle growth and repair; however, the exact role and spatio-temporal expression of Mustn1 have yet to be fully defined. Transgenic mice were generated with a 1512-bp sequence of the Mustn1 promoter directing the expression of GFP (Mustn1PRO-GFP). These mice were used to investigate the sp...
Article
Microgravity (MG) is known to induce bone loss in astronauts during long-duration space mission because of a lack of sufficient mechanical stimulation under MG. It has been demonstrated that mechanical signals are essential for maintaining cell viability and motility, and they possibly serve as a countermeasure to the catabolic effects of MG. The o...
Article
Full-text available
Abstract Gold nanoparticles (AuNPs) are used in various fields; however, their potential health risk(s) are still under the question. In this manuscript, we describe the interactions of AuNPs with human adipose derived stromal cells (ADSCs) and show that they are able to penetrate cell membrane and are stored in vacuoles. As expected, it was found...
Article
Full-text available
RNA interference (RNAi) is a promising approach for cancer treatment. Site specific and controlled delivery of RNAi could be beneficial to the patient, while at the same time reducing undesirable off-target side effects. We utilized electrospinning to generate a biodegradable scaffold capable of incorporating and delivering a bioactive plasmid enco...
Article
Compact fluorescent light (CFL) bulbs can provide the same amount of lumens as incandescent light bulbs, using one quarter of the energy. Recently, CFL exposure was found to exacerbate existing skin conditions; however, the effects of CFL exposure on healthy skin tissue have not been thoroughly investigated. In this study, we studied the effects of...