Michael Gelinsky

Michael Gelinsky
Technische Universität Dresden | TUD · Centre for Translational Bone, Joint and Soft Tissue Research

Prof. Dr. rer. nat.

About

375
Publications
71,514
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
9,768
Citations
Additional affiliations
November 2010 - present
Technische Universität Dresden
Position
  • Head of Faculty
May 2002 - October 2010
Max Bergmann Center of Biomaterials (MBC)
Max Bergmann Center of Biomaterials (MBC)
Position
  • Group Leader
January 1999 - present
Technische Universität Dresden
Position
  • Research Assistant

Publications

Publications (375)
Article
Full-text available
The fabrication of patient-specific scaffolds for bone substitutes is possible through extrusion-based 3D printing of calcium phosphate cements (CPC) which allows the generation of structures with a high degree of customization and interconnected porosity. Given the brittleness of this clinically approved material, the stability of open-porous scaf...
Article
Full-text available
Three-dimensional printing technologies exhibit tremendous potential in the advancing fields of tissue engineering and regenerative medicine due to the precise spatial control over depositing the biomaterial. Despite their widespread utilization and numerous advantages, the development of suitable novel biomaterials for extrusion-based 3D printing...
Article
Mechanical stimulation of bioprinted constructs can enhance the differentiation of cells within these scaffolds, such as driving chondrocytes towards cartilage tissue substitutes. In this study, a holistic approach is presented for designing and engineering a material-specific device based on a magnetic field setup using the Maxwell configuration f...
Article
Full-text available
Long-term stability of gellan gum (GG) at physiological conditions is expected, as very low concentration of divalent ions are required for crosslinking, as compared to alginate—which is extensively used for tissue engineering (TE) applications. Hence, GG is proposed as an ideal candidate to substitute alginate for TE. Deacylated (low acyl; LA) GG...
Article
Full-text available
To treat critical-size bone defects, composite materials and tissue-engineered bone grafts play important roles in bone repair materials. The purpose of this study was to investigate the bone regenerative potential of hybrid scaffolds consisting of macroporous calcium phosphate cement (CPC) and microporous mineralized collagen matrix (MCM). Hybrid...
Article
3D bioprinting – the fabrication of geometrically complex 3D structures from biocompatible materials containing living cells using additive manufacturing technologies – is a rapidly developing research field with a broad range of potential applications in fundamental research, regenerative medicine and industry. Currently, research into 3D bioprint...
Article
In vitro triple cultures of human primary osteoblasts, osteocytes and osteoclasts can potentially help to analyze the effect of drugs and degradation products of biomaterials as a model for native bone tissue. In the present study, degradation products of Magnesium (Mg), which has been successfully applied in the biomedical field, were studied with...
Article
Calcium phosphate cements (CPC) and mesoporous bioactive glasses (MBG) are two well studied biomaterial groups widely under investigation on their applicability to treat bone defects in orthopaedics and maxillofacial surgery. Recently the extrusion properties of CPC-MBG composites using a pasty CPC based on a hydrophobic carrier-liquid were studied...
Article
Full-text available
Besides osteoconductivity and a high degradation rate, mesoporous bioactive glasses (MBGs) are specific for their highly ordered channel structure and high specific surface area, making them suitable as drug and/or growth factor delivery systems. On the other hand, the mesoporous channel structure and MBG composition can have an effect on common ce...
Article
Bänder- und Sehnenrisse führen zu Schmerzen, Bewegungseinschränkungen, Beeinträchtigungen der Mobilität und der Lebensqualität. Daher wurde ein simulationsbasierter Ansatz angewandt, um eine flexible Technologie zur Anpassung von Textilimplantaten an die natürliche anatomische Form und das biomechanische Verhalten von Sehnen und Bändern über strukt...
Article
Additive manufacturing is a promising technology for the fabrication of customized implants with complex geometry. The objective of this study was to investigate the initial cell–material interaction of degradable Fe-30Mn-1C-0.02S stent structures in comparison to conventional 316L as a reference, both processed by laser powder bed fusion. FeMn-bas...
Article
Full-text available
One of the key challenges in osteochondral tissue engineering is to define specified zones with varying material properties, cell types and biochemical factors supporting locally adjusted differentiation into the osteogenic and chondrogenic lineage, respectively. Herein, extrusion-based core-shell bioprinting is introduced as a potent tool allowing...
Article
Full-text available
Soft tissue infections in open fractures or burns are major cause for high morbidity in trauma patients. Sustained, long-term and localized delivery of antimicrobial agents is needed for early eradication of these infections. Traditional (topical or systemic) antibiotic delivery methods are associated with a variety of problems, including their lon...
Article
Full-text available
Highly viscous bioinks offer great advantages for the three-dimensional fabrication of cell-laden constructs by microextrusion printing. However, no standardised method of mixing a high viscosity biomaterial ink and a cell suspension has been established so far, leading to non-reproducible printing results. A novel method for the homogeneous and re...
Chapter
The chapter describes how Additive Manufacturing (AM) is used in tissue engineering, both for fabrication of polymeric or ceramic scaffolds and for the direct generation of cell-laden constructs by means of 3D Bioprinting. The most important AM technologies for these applications are introduced and the main advantages like the opportunity to fabric...
Article
Full-text available
Although bone morphogenetic protein-2 (BMP-2) is widely used for spinal fusion, some concerns cannot be ignored. The combination of growth factor with composite scaffolds represent a promising approach to promote bone regeneration and spinal fusion. Recent studies have demonstrated that the growth differentiation factor-5 (GDF-5) variant BB-1, comb...
Article
Skin wound healing is known to be impaired in space. As skin is the tissue mostly at risk to become injured during manned space missions, there is the need for a better understanding of the biological mechanisms behind the reduced wound healing capacity in space. In addition, for far-distant and long-term manned space missions like the exploration...
Article
Full-text available
The replacement of damaged or degenerated articular cartilage tissue remains a challenge, as this non-vascularized tissue has a very limited self-healing capacity. Therefore, tissue engineering (TE) of cartilage is a promising treatment option. Although significant progress has been made in recent years, there is still a lack of scaffolds that ensu...
Article
Full-text available
Magnetic resonance imaging (MRI) is a common clinical practice to visualize defects and to distinguish different tissue types and pathologies in the human body. So far, MRI data have not been used to model and generate a patient-specific design of multilayered tissue substitutes in the case of interfacial defects. For orthopedic cases that require...
Article
Full-text available
In vitro evaluation of bone graft materials is generally performed by analyzing the interaction with osteoblasts or osteoblast precursors. In vitro bone models comprising different cell species can give specific first information on the performance of those materials. In the present study, a 3D co-culture model was established comprising primary hu...
Article
Moulded hydrogels reinforced with melt-electrowritten (MEW) microfibres to tailor mechanical properties show promise in the articular cartilage regeneration field. We aim to transfer this knowledge to bioprinted constructs of volumetric dimensions towards real anatomical shapes, using a previously established alginate methylcellulose (algMC) blend....
Chapter
Design und Fertigung individueller, künstlicher Ersatzgewebe sind große Herausforderungen der biomedizinischen Forschung. Extrusionsbasierte additive Fertigungsmethoden eröffnen neue Möglichkeiten, um Biomaterialien nach einem individuellen Design anzuordnen. Der Schwerpunkt aktueller Forschungstätigkeiten liegt in der Generierung individueller bio...
Article
Full-text available
To develop cost-effective and efficient bone substitutes for improved regeneration of bone defects, heparin-modified mineralized collagen scaffolds were functionalized with concentrated, naturally occurring bioactive factor mixtures derived from adipose tissue, platelet-rich plasma and conditioned medium from a hypoxia-treated human bone marrow-der...
Article
Full-text available
Human immune system acts as a pivotal role in the tissue homeostasis and disease progression. Immunomodulatory biomaterials that can manipulate innate immunity and adaptive immunity hold great promise for a broad range of prophylactic and therapeutic purposes. This review is focused on the design strategies and principles of immunomodulatory biomat...
Article
Full-text available
Bioactive glasses have been used for bone regeneration applications thanks to their excellent osteoconductivity, an osteostimulatory effect, and high degradation rate, releasing biologically active ions. Besides these properties, mesoporous bioactive glasses (MBG) are specific for their highly ordered mesoporous channel structure and high specific...
Article
Full-text available
With the aim of understanding and recapitulating cellular interactions of hepatocytes in their physiological microenvironment and to generate an artificial 3D in vitro model, a co-culture system using 3D extrusion bioprinting was developed. A bioink based on alginate and methylcellulose (algMC) was first shown to be suitable for bioprinting of hepa...
Article
Currently, we are experiencing a true pandemic of a communicable disease by the virus SARS-CoV-2 holding the whole world firmly in its grasp. Amazingly and unfortunately, this virus uses a metabolic and endocrine pathway via ACE2 to enter our cells causing damage and disease. Our international research training programme funded by the German Resear...
Article
Full-text available
Background: Copper-containing biomaterials are increasingly applied for bone regeneration due to their pro-angiogenetic, pro-osteogenetic and antimicrobial properties. Therefore, the effect of Cu2+ on osteoclasts, which play a major role in bone remodeling was studied in detail. Methods: Human primary osteoclasts, differentiated from human monoc...
Article
This research investigated 3D printed scaffolds of alginate/polyvinylalcohol incorporated with silk fibroin (SF) fabricated by mimicking soft materials similar to bone extracellular matrix. The alginate/polyvinyl alcohol incorporated with 0%, 0.5%, 1%, and 2% SF were fabricated into 3D printed scaffolds. The morphology was observed using scanning e...
Article
Mechanical stimulation of cells embedded in scaffolds is known to increase the cellular performance toward osteogenic or chondrogenic differentiation and tissue development. Three-dimensional bioplotting of magnetically deformable scaffolds enables the spatially defined distribution of magnetically inducible scaffold regions. In this study, a magne...
Article
Full-text available
The tympanic membrane (TM) transfers sound waves from the air into mechanical motion for the ossicular chain. This requires a high sensitivity to small dynamic pressure changes and resistance to large quasi-static pressure differences. The TM achieves this by providing a layered structure of about 100µm in thickness, a low flexural stiffness, and a...
Article
Full-text available
Cleft alveolar bone defects can be treated potentially with tissue engineered bone grafts. Herein, we developed novel biphasic bone constructs consisting of two clinically certified materials, a calcium phosphate cement (CPC) and a fibrin gel that were biofabricated using 3D plotting. The fibrin gel was loaded with mesenchymal stromal cells (MSC) d...
Article
Full-text available
In vitro evaluation of bone graft materials is generally performed by analysing the interaction with osteoblasts or osteoblast precursors. In vitro bone models comprising different cell species can give more specific first information on the performance of those materials after being implanted. In the present study, a 3D co-culture model was establ...
Article
Full-text available
Bioceramics have been developed from bioinert to bioactive or biodegradable materials in the past few decades. However, at present, traditional bioceramics are still mainly used in bone tissue regeneration and dental restoration. In this work, a new generation of “black bioceramics,” extending the applications from tissue regeneration to disease th...
Article
25 years after the first Berlin Workshop on Developmental Toxicity this 10th Berlin Workshop aimed to bring together international experts from authorities, academia and industry to consider scientific, methodologic and regulatory aspects in risk assessment of developmental toxicity and to debate alternative strategies in testing developmental effe...
Article
Fifty years after the first human landed on the Moon mankind has started to plan next steps for manned space exploration missions. The international space agencies have begun to investigate the requirements for both a human settlement on the Moon and manned missions to Mars. For such activities significantly improved medical treatment facilities on...
Article
Full-text available
The proteasome inhibitor bortezomib (BZM) is one of the most potent anti-cancer drugs in the therapy of multiple myeloma. In this study, an adhesive drug delivery system (DDS) for BZM was developed. Therefore, we extended the present DDS concept of polyelectrolyte complex (PEC) nanoparticle (NP) based on electrostatic interactions between charged d...
Article
Full-text available
The development of an engineered non-contact multicellular coculture model that can mimic the in vivo cell microenvironment of human tissues remains challenging. In this study, we successfully fabricated a cell-container-like scaffold composed of β-tricalcium phosphate/hydroxyapatite (β-TCP/HA) bioceramic that contains four different pore structure...
Article
Full-text available
Molluscan shells are an example of a mineral-based biocomposite material, and most studies to date have focused on understanding their biomineralization mechanisms. Meanwhile, large amounts of these shells are produced as waste globally by seafood which is used by other industries as a source of biogenic calcium carbonates. In this study, we propos...
Article
The present work focuses on the application of time-of-flight secondary ion mass spectrometry (ToF-SIMS) in osteoporotic bone research. In order to demonstrate the benefit, the authors present concrete application examples of ToF-SIMS in three different areas of bone research. ToF-SIMS as a mass spectrometric imaging technique allows simultaneous v...
Article
Full-text available
For the generation of multi-layered full thickness osteochondral tissue substitutes with an individual geometry based on clinical imaging data, combined extrusion-based 3D printing (3D plotting) of a bioink laden with primary chondrocytes and a mineralized biomaterial phase was introduced. A pasty calcium phosphate cement (CPC) and a bioink based o...
Article
A new approach for additive manufacturing of individual implants is the utilisation of hydrogels with stem cells and magnetic micro-particles. Due to the magnetic particles, the implant can be deformed in an alternating magnetic field, whereby a controlled differentiation of the stem cells is possible. In this study, a matrix material of alginate-m...
Article
Full-text available
Three-dimensional (3D) biopolymer-based scaffolds including chitinous matrices have been widely used for tissue engineering , regenerative medicine and other modern interdisciplinary fields including extreme biomimetics. In this study, we introduce a novel, electrochemically assisted method for 3D chitin scaffolds isolation from the cultivated mari...
Article
The present work focuses on the application of time-of-flight secondary ion mass spectrometry (ToF-SIMS) in osteoporotic bone research. In order to demonstrate the benefit, the authors present concrete application examples of ToF-SIMS in three different areas of bone research. ToF-SIMS as a mass spectrometric imaging technique allows simultaneous v...
Article
Full-text available
Acellular soft hydrogels are not ideal for hard tissue engineering given their poor mechanical stability, however, in combination with cellular components offer significant promise for tissue regeneration. Indeed, nanocomposite bioinks provide an attractive platform to deliver human bone marrow stromal cells (HBMSCs) in three dimensions producing c...
Article
Full-text available
For cementless total joint replacement implants, the biological response to physicochemical surface characteristics is crucial for their success that depends on fixation by newly formed bone. In this study, the surface of TiAl6V4 (Tilastan®) implants was modified by (a) corundum blasting, (b) corundum blasting followed by electrochemical calcium ph...
Article
Full-text available
3D printing alias additive manufacturing can transform 3D virtual models created by computer-aided design (CAD) into physical 3D objects in a layer-by-layer manner dispensing with conventional molding or machining. Since the incipiency, significant advancements have been achieved in understanding the process of 3D printing and the relationship of c...
Article
Full-text available
Introduction Bone marrow stromal cells (BMSC) are highly attractive for tissue engineering due to their ability to differentiate into different cell types, to expand extensively in vitro and to release paracrine soluble factors with a high regenerative potential. They were observed to migrate towards the sites of injury in response to chemotactic s...
Article
Full-text available
With the aid of biofabrication, cells can be spatially arranged in three dimensions, which offers the opportunity to guide tissue maturation in a better way compared to traditional tissue engineering approaches. A prominent technqiue allowing biofabrication of tissue equivalents is extrusion-based 3D (bio)printing, also called 3D (bio)plotting or r...
Article
Full-text available
One of the most common hereditary craniofacial anomalies in humans are cleft lip and cleft alveolar bone with or without cleft palate. Current clinical practice, the augmentation of the persisting alveolar bone defect by using autologous bone grafts, has considerable disadvantages motivating to an intensive search for alternatives. We developed a n...
Conference Paper
Additive Manufacturing of biomaterials is typically based on extrusion-based methods: A pasty material is extruded from a cartridge building up a volumetric structure (scaffold) in a layer-by-layer fashion through defined travel movements. The porosity of such objects-a critical factor triggering mechanical and bioactive properties-is primarily det...
Article
Extrusion-based bioprinting, also known as 3D bioplotting, is a powerful tool for the fabrication of tissue equivalents with spatially defined cell distribution. Even though considerable progress has been made in recent years, there is still a lack of bioinks which enable a tissue-like cell response and are plottable at the same time with good shap...
Article
Full-text available
In this study, the bone-regenerative potential of bioactive factors derived from adipose tissue, platelet-rich plasma (PRP) and conditioned medium from hypoxia-treated human telomerase immortalized bone-marrow-derived mesenchymal stem cells (hTERT-MSC) was investigated in vitro with the aim to develop cost-effective and efficient bone substitutes f...
Article
Full-text available
Systematic analysis of the extrusion process in 3D bioprinting is mandatory for process optimization concerning production speed, shape fidelity of the 3D construct and cell viability. In this study, we applied numerical and analytical modelling to describe the fluid flow inside the printing head based on a Herschel-Bulkley model. The presented ana...