Michael Ben-Yosef

Michael Ben-Yosef
Agricultural Research Organization ARO | aro · Department of Entomology, Nematology and Chemistry

Ph.D.
https://michaelb759.wixsite.com/insect-physiology

About

19
Publications
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Introduction
I'm studying insect – microbe systems of agricultural importance, with particular interest in fruit flies (Tephritidae and Drosophilidae). Within this frame, I'm aiming to demonstrate how microbes contribute to insect phenotypes and behavior which are relevant to agroecosystems, e.g. host plant utilization, resistance to abiotic stress and insecticides, and attraction. Our work combines insect bioassays with NGS community sequencing, molecular and classic microbiology and biochemical analyses.

Publications

Publications (19)
Article
Full-text available
Here we compare the Coxiella -like endosymbionts (CLE) proteomes from Malpighian tubule (Mt) and the ovaries (Ov) of the brown dog tick Rhipicephalus sanguineus . Our results support the hypothesis that CLEs function interdependently with host requirements in each of the organs.
Article
Full-text available
Insects, similarly to other small terrestrial invertebrates, are particularly susceptible to climatic stress. Physiological adjustments to cope with the environment (i.e., acclimation) together with genetic makeup eventually determine the tolerance of a species to climatic extremes, and constrain its distribution. Temperature and desiccation resist...
Article
Domestication disconnects an animal from its natural environment and diet, imposing changes in the attendant microbial community. We examine these changes in Philornis downsi (Muscidae), an invasive parasitic fly of land birds in the Galapagos Islands. Using a 16S rDNA profiling approach we studied the microbiome of larvae and adults of wild and la...
Article
Full-text available
Obligatory hematophagous arthropods such as lice, bugs, flies, and ticks harbor bacterial endosymbionts that are expected to complement missing essential nutrients in their diet. Genomic and some experimental evidence support this expectation. Hard ticks (Acari: Ixodidae) are associated with several lineages of bacterial symbionts, and very few wer...
Article
Full-text available
Background: The Mediterranean fruit fly Ceratitis capitata is a major pest in horticulture. The development of fly larvae is mediated by bacterial decay in the fruit tissue. Despite the importance of bacteria on larval development, very little is known about the interaction between bacteria and larvae in their true ecological context. Understandin...
Article
Full-text available
Philornis downsi Dodge and Aitken (Diptera: Muscidae) is an avian parasitic fly that has invaded the Galapagos archipelago and exerts an onerous burden on populations of endemic land birds. As part of an ongoing effort to develop tools for the integrated management of this fly, our objective was to determine its long- and short-range responses to b...
Article
Microbial associations are widespread across the insects. In the olive fruit fly Bactrocera oleae (Diptera: Tephritidae), vertically transmitted gut symbionts contribute to larval development inside the olive host, and to adult nutrition. Nevertheless, their effect on behavioural decisions of adults is unknown. In this study, we show that symbiotic...
Preprint
Full-text available
Philornis downsi (Diptera: Muscidae) is a nest parasitic fly that has invaded the Galapagos archipelago and exerts an onerous burden on populations of endemic land birds. As part of an ongoing effort to develop tools for the integrated management of this fly, our objective was to determine its long and short-range responses to bacterial and yeast c...
Preprint
Full-text available
Background: The Mediterranean fruit fly Ceratitis capitata is a major pest in horticulture. The development of fly larvae is mediated by bacterial decay in the fruit tissue. Despite the importance of bacteria on larval development, very little is known about the interaction between bacteria and larvae in their true ecological context. Understanding...
Article
Full-text available
The composition and diversity of bacteria forming the microbiome of parasitic organisms have implications for differential host pathogenicity and host-parasite co-evolutionary interactions. The microbiome of pathogens can therefore have consequences that are relevant for managing disease prevalence and impact in affected hosts. Here we investigate...
Article
Full-text available
The olive fruit fly, Bactrocera oleae, is the most destructive pest of olive orchards worldwide. The monophagous larva has the unique capability of feeding on olive mesocarp, coping with high levels of phenolic compounds and utilizing non-hydrolyzed proteins present, particularly in the unripe, green olives. On the molecular level, the interaction...
Article
Full-text available
“ Candidatus Erwinia dacicola” is a Gammaproteobacterium that forms a symbiotic association with the agricultural pest Bactrocera oleae . Here, we present a 2.1-Mb draft hybrid genome assembly for “ Ca. Erwinia dacicola” generated from single-cell and metagenomic data.
Article
Full-text available
Ripe fruit offer readily available nutrients for many animals, including fruit fly larvae (Diptera: Tephritidae) and their associated rot-inducing bacteria. Yet, during most of their ontogeny, fruit remain chemically defended and effectively suppress herbivores and pathogens by high levels of secondary metabolites. Olive flies (Bactrocera oleae) ar...
Article
Insects are often associated with symbiotic micro-organisms, which allow them to utilize nutritionally marginal diets. Adult fruit flies (Diptera: Tephritidae) associate with extracellular bacteria (Enterobacteriaceae) that inhabit their digestive tract. These flies obtain nutrients by foraging for plant exudates, honeydew and bird droppings scatte...
Article
Full-text available
The Mediterranean fruit fly (medfly) (Ceratitis capitata) lays eggs in fruits, where larvae subsequently develop, causing large-scale agricultural damage. Within its digestive tract, the fly supports an extended bacterial community that is composed of multiple strains of a variety of enterobacterial species. Most of these bacteria appear to be func...
Article
Mediterranean fruit flies (Ceratitis capitata Wiedemann, Diptera: Tephritidae), harbour a diverse community of bacteria in their digestive system. Molecular and culture-based techniques show that members of the Enterobacteriaceae form the dominant populations in the gut of the Mediterranean fruit fly. Among them, many are diazotrophs and actively f...
Article
Olive flies (Bactrocera oleae) are intimately associated with bacteria throughout their life cycle, and both larvae and adults are morphologically adapted for housing bacteria in the digestive tract. We tested the hypothesis that these bacteria contribute to the adult fly's fitness in a diet-dependent fashion. We predicted that when dietary protein...
Article
Mediterranean fruit flies (Ceratitis capitata Wiedemann, Dipt.: Tephritidae) harbour a diverse community of bacteria in their digestive system. This microbiota may have important functions impacting on the fly’s fitness. Recently, we described the effect of eliminating intestinal bacteria on the reproductive success of C. capitata males and females...
Article
Abstract Mediterranean fruit flies (Ceratitis capitata Wiedemann, Diptera: Tephritidae) harbor a community of diazotrophic bacteria in their digestive system. The present study aims to test the hypothesis that bacteria contribute to fly fitness by enhancing copulatory success and egg production in males and females, respectively. After eclosion, fl...

Projects

Projects (3)
Project
To examine the ecological, behavioral and evolutionary aspects of olive fly- bacteria symbiosis comparatively to other fruit flies
Project
To characterize the sour rot system in vineyards in Israel and examine the complementary association between larvae and their microbiome