Micaela Liberti

Micaela Liberti
Sapienza University of Rome | la sapienza · Department of Information Engineering, Electronics and Telecommunications

PhD

About

271
Publications
25,882
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Introduction
Micaela Liberti is Associate Professor at DIET, University "La Sapienza" of Rome. Her research concerns the mechanisms of interaction between EM fields and biological systems, microdosimetry, the design of exposure systems and dosimetry. Since 2008 she is in the Scientific Council of the European Bioelectromagnetics Association (EBEA). In 2011 she acted as General Chair of EBEA2011, she is in COST TD1104: European network for development of electroporation-based technologies and treatments.
Additional affiliations
November 2019 - December 2020
Sapienza University of Rome
Position
  • Professor (Associate)

Publications

Publications (271)
Article
Purpose: This study aims to perform a classification and rigorous numerical evaluation of the risks of occupational exposure in the health environment related to the administration of Transcranial Magnetic Stimulation (TMS) treatment. The study investigates the numerically estimated induced electric field that occurs in the human tissues of an ope...
Article
Full-text available
Stimuli-sensitive nanocarriers have recently been developed as a powerful tool in biomedical applications such as drug delivery, detection, and gene transfer techniques. Among the external triggers investigated, low intensity magnetic fields represent a non-invasive way to remotely control the release of compounds from a magneto-sensitive carrier....
Article
Electroporation is a well-established technique used to stimulate cells, enhancing membrane permeability by inducing reversible membrane pores. In the absence of experimental observation of the dynamics of pore creation, molecular dynamics studies provide the molecular-level evidence that the electric field promotes pore formation. Although single...
Article
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The aim of this study was to investigate quantitatively local sub-cellular power deposition at frequencies upcoming for wireless power transfer (WPT) and millimeter-wave (mmWave) technologies. The study was performed on a realistic two-dimensional keratinocyte cell model, designed based on electron microscopy images and experimental data on surface...
Article
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Objectives Acute cerebral ischemia is characterized by several pathological processes evolving during time, which contribute to the final tissue damage. Secondary processes, such as prolonged inflammatory response, impaired mitochondrial function and oxidative stress, are responsible for the progression of brain injury to the peri-infarct area, cal...
Article
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Low-intensity focused ultrasound is emerging as a high-resolution highly selective alternative to standard noninvasive transcranial brain stimulation techniques. A major challenge in using ultrasound devices is designing a stimulator capable of efficiently focusing the acoustic wave to selectively target a specific brain region by compensating for...
Article
Full-text available
Electroporation has become a powerful technological platform for the electromanipulation of cells and tissues for various medical and biotechnological applications. Recently, nanoporation based on nanosecond pulsed electric fields (nsPEFs) has gained great attention due to its potential to permeabilize the membrane of small vesicles. Here, the auth...
Article
This study aims at quantifying the effect that using different skin conductivity values has on the estimation of the electric (E)-field distribution induced by transcranial magnetic stimulation (TMS) and transcranial direct current stimulation (tDCS) in the brain of two anatomical models. The induced E-field was calculated with numerical simulation...
Chapter
Full-text available
Transcranial magnetic stimulation (TMS) is a noninvasive brain stimulation technique used in the clinic to treat several neurological disorders and psychiatric diseases. One of TMS’s significant limitations is its low spatial resolution, which often results in a mismatch between the target area in the brain and the stimulation site on the scalp. To...
Article
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In the last years, microdosimetric numerical models of cells including intracellular compartments have been proposed, aiming to investigate the poration induced by the application of nanosecond pulsed electric fields (nsPEFs). A limitation of such models was the extremely approximate cell and organelle shapes, leading to an incorrect estimation of...
Article
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The increasing interest toward biocompatible nanotechnologies in medicine, combined with electric fields stimulation, is leading to the development of electro-sensitive smart systems for drug delivery applications. To this regard, recently the use of pulsed electric fields to trigger release across phospholipid membranes of liposomes has been numer...
Conference Paper
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Non-contact galvanotaxis as a way to drive the cells migration could be a promising tool for a variety of biomedical applications, such as wound healing control, avoiding the interaction between electrodes and cell cultures. To this regard, the efficacy of this electrical stimulus application has to be deeper studied to control physiological migrat...
Conference Paper
Electroporation is a well-established technique used to stimulate cells, enhancing membrane permeability. Although the biological phenomena occurring after the poration process have been widely studied, the physical mechanisms of pore formation are not clearly understood. In this work we investigated by means of molecular dynamics simulations the k...
Article
Full-text available
Power spectra of spike trains reveal important properties of neuronal behavior. They exhibit several peaks, whose shape and position depend on applied stimuli and intrinsic biophysical properties, such as input current density and channel noise. The position of the spectral peaks in the frequency domain is not straightforwardly predictable from sta...
Article
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Mechanisms of how electromagnetic (EM) field acts on biological systems are governed by the same physics regardless of the origin of the EM field (technological, atmospheric...), given that EM parameters are the same. We draw from a large body of literature of bioeffects of a man-made electromagnetic field. In this paper, we performed a focused rev...
Article
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Neuroprotective effects of pulsed electromagnetic fields (PEMFs) have been demonstrated both in vivo and in vitro. Moreover, preliminary clinical studies have been conducted and suggested PEMFs as a possible alternative therapy to treat acute ischemic stroke. In this work, we show that it’s possible to build-up a patient semi-specific head model, w...
Article
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The initiation of action potentials (APs) by membrane depolarization occurs after a brief vulnerability period, during which excitation can be abolished by the reversal of the stimulus polarity. This vulnerability period is determined by the time needed for gating of voltage-gated sodium channels (VGSC). We compared nerve excitation by ultra-short...
Article
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Electric fields can be a powerful tool to interact with enzymes or proteins, with an intriguing perspective to allow protein manipulation. Recently, researchers have focused the interest on intracellular enzyme modifications triggered by the application of nanosecond pulsed electric fields. These findings were also supported by theoretical predicti...
Article
Full-text available
Intense pulsed electric fields are known to act at the cell membrane level and are already being exploited in biomedical and biotechnological applications. However, it is not clear if electric pulses within biomedically-attainable parameters could directly influence intra-cellular components such as cytoskeletal proteins. If so, a molecular mechani...
Conference Paper
The increasing interest towards biocompatible nanotechnologies in medicine, combined with electric fields stimulation, is leading to the development of electro-sensitive smart systems for drug delivery applications. Common examples of electro-sensitive materials include phospholipids that can be used to design nano-sized vesicles suitable for exter...
Conference Paper
Numerical evaluation of the electromagnetic (EM) quantities induced inside the brain during transcranial magnetic stimulation (TMS) applications is a fundamental step to obtain the optimization of the treatment in terms of coil position and current intensity. In this sense, the human head model considered and the electromagnetic properties used to...
Conference Paper
A new miniaturized figure-of-eight coil (μCoil) for TMS applications has been developed taking advantage of the Flex circuit technology. First experiments on volunteers demonstrated the ability of the μCoil to elicit sensorial action potentials of the peripheral nervous system. The necessity of reducing the size of standard TMS stimulator arises fr...
Conference Paper
When investigating the biophysical effects induced by the interaction between electromagnetic fields and biological cells, it is crucial to estimate the electromagnetic field intensity at the microscopic scale (microdosimetry). This information allows to find a connection between the external applied field and the observed biological event required...
Article
Background: Molecular mechanisms of interaction between cells and extremely low frequency magnetic fields (ELF-MFs) still represent a matter of scientific debate. In this paper, to identify the possible primary source of oxidative stress induced by ELF-MF in SH-SY5Y human neuroblastoma cells, we estimated the induced electric field and current den...
Preprint
Full-text available
Intense pulsed electric fields are known to act at the cell membrane level and are already being exploited in biomedical and biotechnological applications. However, it is not clear if intra-cellular components such as cytoskeletal proteins could be directly influenced by electric pulses within biomedically-attainable parameters. If so, a molecular...
Article
Full-text available
Electroporation is a matter of intensive ongoing research interest, and a much-neglected topic in trans-membrane proteins, particularly in view of such promising potential applications in medicine and biotechnology. In particular, selected such novel and exciting applications are predicated on controlling ionic conductivity through electro-pores. H...
Article
Human aquaporin 4 has been studied using non-equilibrium molecular dynamics simulations in the absence and presence of pulses of external electric fields. The pulses were 100 ns in duration and 0.005–0.015 V/Å in intensity acting along the pores’ axes. Water diffusivity and the dipolar response of various residues of interest within the pores have...
Article
The use of magnetic fields in therapeutic applications has considerably increased in recent years. In particular, many researchers have focused their attention on the use of low-intensity magnetic fields, either alone or in combination with nanoparticles for drug delivery systems in nanomedicine. Laboratory experiments aimed at defining in vitro...
Article
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Electroporation characterisation is a topic of intensive interest probed by extensive ongoing research efforts. Usually, these studies are carried out on lipid-bilayer electroporation. Surprisingly, the possibility of water-channel electropore formation across trans-membrane proteins themselves, particularly in view of such a promising application,...
Article
Full-text available
Tinnitus is a debilitating perception of sound in the absence of external auditory stimuli. It may have either a central or a peripheral origin in the cochlea. Experimental studies evidenced that an electrical stimulation of peripheral auditory fibers may alleviate symptoms but the underlying mechanisms are still unknown. In this work, a stochastic...
Article
A processing technique for decoding the information transferred from a sinusoidal input to the output spike sequence of a neuron model is a desirable tool for understanding the encoding principles of neuronal systems. An automatic decoding procedure, already proposed by the authors, is based on an improved version of the Signal to Noise Ratio (SNR)...
Article
Full-text available
Recently, magnetic nanoparticles (MNPs) have been used to trigger drug release from magnetoliposomes through a magneto-nanomechanical approach, where the mechanical actuation of the MNPs is used to enhance the membrane permeability. This result can be effectively achieved with low intensity non-thermal alternating magnetic field (AMF), which, howev...
Article
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The analytical and numerical design, implementation, and experimental validation of a new grounded closed coplanar waveguide for wide-band electromagnetic exposures of cells and their optical detection in real-time is reported. The realized device fulfills high-quality requirements for novel bioelectromagnetic experiments, involving elevated tempor...
Article
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Microsecond pulsed electric fields (μsPEF) permeabilize the plasma membrane (PM) and are widely used in research, medicine and biotechnology. For internal membranes permeabilization, nanosecond pulsed electric fields (nsPEF) are applied but this technology is complex to use. Here we report that the endoplasmic reticulum (ER) membrane can also be el...
Article
Full-text available
Extremely low frequency magnetic fields (ELF-MF) could be an alternative neuroprotective approach for ischemic stroke because preclinical studies have demonstrated their effects on the mechanisms underlying ischemic damage. The purpose of this open-label, one arm, dose-escalation, exploratory study is to evaluate the safety and tolerability of ELF-...
Chapter
Molecular dynamics (MD) simulations recently proved to be a useful tool for unveiling many aspects of pore formation in lipid membranes under the influence of external electric fields. In particular, the study of the structure and transport properties of electropores must definitely take advantage of a rigorous characterization of pore geometry and...
Article
Full-text available
Molecular dynamics (MD) simulations have proved to be a useful tool for unveiling many aspects of pore formation in lipid membranes under the influence of external electric fields. In order to compare the size-related properties of pores in bilayers of various compositions, generated and maintained under different physical and chemical conditions,...