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119
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Introduction
I am working as a plant breeder in Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad, Pakistan. I am working on wheat crop in addition to barley. My area of expertise include plant breeding, genetics, pathology, agronomy, seed science and related disciplines. I am always available for research collaboration and open to new ideas. You can reach me through m.a.rehman.arif@gmail.com or via telephone at 0092-333-5521394
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Publications
Publications (119)
Cadmium (Cd) contamination poses a significant threat to global agricultural production and hyper accumulating plant species, such as Solanum nigrum (L.), face challenges in phytoremediation due to limited biomass production. Application of plant growth regulators such as folic acid (FA) is a promising strategy to increase biomass production in the...
The size, shape, and color of wheat seeds are important traits that are associated with yield and flour quality (size, shape), nutritional value, and pre-harvest sprouting (coat color). These traits are under multigenic control, and to dissect their molecular and genetic basis, quantitative trait loci (QTL) analysis is used. We evaluated 114 recomb...
Ginger, a fresh rhizome, an economically important spice with extensive nutraceutical activities finds itself in vegetable and therapeutic market. Aflatoxins (AFB1, AFB2, AFG1 and AFG2) along with ochratoxin A (OTA) are the most significant and the most toxic form of mycotoxins which are produced by various fungi. This study was initiated to assess...
Yield losses because of cereal cyst nematodes could be as high as 92%, causing a bottleneck for wheat production. An integrated approach (application of pesticides, crop rotation, and use of host resistance) is needed to manage this devastating pathogen where resistant cultivars are considered most effective. This necessitates the identification of...
Pre-cleaned wheat grains were milled to produce whole wheat flour. Cleaned barley grains were soaked in water overnight which were dried till the desirable moisture of 9.0 % and milled to produce barley flour. After this, composite flour of wheat and soaked barley was made at a combination of 0, 5, 10, 15, 20, and 25% barley in the whole wheat flou...
Climate change has negatively impacted lentil growth and production, necessitating advanced omics tools for trait characterization and genetic improvement. Traditional breeding methods have reached their limits, making high-throughput omics technologies, biotechnology, and bioinformatics essential. Despite their benefits, efforts targeting lentils...
A number of consequences of climate change, notably salinity, put global food security at risk by impacting the development and production of lentils. Salinity-induced stress alters lentil genetics, resulting in severe developmental issues and eventual phenotypic damage. Lentils have evolved sophisticated signaling networks to combat salinity stres...
This study employed genome‐wide association studies (GWAS) to identify the crucial marker–trait associations (MTAs) for agronomic and physiological traits in bread wheat grown under full irrigation and 40% reduced irrigation. One hundred twenty‐four genotypes derived from three‐way crosses of landraces and synthetic bread wheat were evaluated for 2...
Low nitrogen (N) is a major limitation of cotton sustainability and productivity. N-efficient genotype cultivation can enhance productivity under the economical N usage. This study was conducted to characterize 15 cotton genotypes for N use efficiency (NUE) under differential N (0.0125 and 0.125 g kg −1 of sand) supply. All studied traits significa...
Effective identification and usage of genetic variation are prerequisites for developing nutrient-efficient cultivars. A collection of 94 safflower (Carthamus tinctorius) genotypes (G) was investigated for important morphological and photosynthetic traits at four nitrogen (N) treatments. We found significant variation for all the studied traits exc...
For full list of author affiliations and declarations see end of paper Effective identification and usage of genetic variation are prerequisites for developing nutrient-efficient cultivars. A collection of 94 safflower (Carthamus tinctorius) genotypes (G) was investigated for important morphological and photosynthetic traits at four nitrogen (N) tr...
Genetic enhancement of grain production and quality is a priority in wheat breeding projects. In this study, we assessed two key agronomic traits—grain protein content (GPC) and thousand kernel weight (TKW)—across 179 Bulgarian contemporary and historic varieties and landraces across three growing seasons. Significant phenotypic variation existed f...
Genetic enhancement of grain production and quality is a priority in wheat breeding projects. In this study, we assessed two key agronomic traits - grain protein content (GPC) and thousand kernel weight (TKW) - across 179 Bulgarian contemporary and historic varieties, and landraces in three growing seasons. Significant phenotypic variation existed...
This study aims to evaluate the role of genetic diversity originated from three-way crosses of landraces and synthetic bread wheat derivatives attempted with elite cultivars to identify the genotypes having better grain yield as well as better starch contents under flowering stage drought stress. In total, 50 genotypes comprised of 47 derivatives o...
Grain protein content (GPC) is a key aspect of grain quality, a major determinant of the flour functional properties and grain nutritional value of bread wheat. Exploiting diverse germplasms to identify genes for improving crop performance and grain nutritional quality is needed to enhance food security. Here, we evaluated GPC in a panel of 255 Tri...
Seed longevity is the most important trait in the genebank management system. No seed can remain infinitely viable. There are 1241 accessions of Capsicum annuum L. available at the German Federal ex situ genebank at IPK Gatersleben. C. annuum (Capsicum) is the most economically important species of the genus Capsicum. So far, there is no report tha...
Background
The narrow genetic diversity of chickpea is a serious impediment to modern cultivar creation. Seed storage proteins (SSPs) are stable and have minimal or no degradation when subjected to isolation and SDS-PAGE.
Methods and results
We have characterized SSPs of 436 chickpea genotypes, belonging to nine annual Cicer species, originated fr...
Cumulative oxidation of cellular macromolecules during storage reduces seed longevity. This study was undertaken to unravel the physiological and biochemical changes in rice seeds that contribute to deterioration during storage. Rice seeds maintained at three different seed moisture contents (SMC; 10, 12 and 14%) were stored in airtight glass jars....
This study was directed to incorporate barley flour derived from hulled barley variety "Jau-17" in the whole wheat flour to develop chapatti. Five different samples of flour blend for chapatti production were made by adding barley (at the rate of 0, 5, 10, 15, 20 and 25%) into the whole wheat flour and their proximate analyses and other quality var...
One of the several impediments to increasing wheat productivity challenge is the low genetic diversity in modern wheat. Successful breeding requires the identification of donor germplasm in genetically diverse pools highlighting the importance of selection and use of diverse genotypes in a breeding program. In this investigation, we attempted to ch...
Sustainable global wheat production requires wheat varieties, that are sufficiently resistant to the main wheat diseases. The economically important fungal pathogens worldwide include powdery mildew (PM), yellow rust (YR), leaf rust (LR) and blotch causing pathogens including Septoria nodorum blotch (SNB) and Septoria tritici blotch (STB). Here, we...
High salinity levels affect 20% of the cultivated area and 9%–34% of the irrigated agricultural land worldwide, ultimately leading to yield losses of crops. The current study evaluated seven salt tolerance-related traits at the seedling stage in a set of 138 pre-breeding lines (PBLs) and identified 63 highly significant marker-trait associations (M...
Yellow (YR) and leaf (LR) rusts caused by Puccinia striiformis f. sp. tritici (Pst) and Pucciniatriticina, respectively, are of utmost importance to wheat producers because of their qualitativeand quantitative effect on yield. The search for new loci resistant to both rusts is an ongoingchallenge faced by plant breeders and pathologists. Our invest...
Citation: Saleem, K.; Shokat, S.; Waheed, M.Q.; Arshad, H.M.I.; Arif, M.A.R. A GBS-Based GWAS Analysis of Leaf and Stripe Rust Resistance in Diverse Pre-Breeding Germplasm of Bread Wheat (Triticum aestivum L.).
Yellow (YR) and leaf (LR) rusts caused by Puccinia striiformis f. sp. tritici (Pst) and Puccinia triticina, respectively, are of utmost importance to wheat producers because of their qualitative and quantitative effect on yield. The search for new loci resistant to both rusts is an ongoing challenge faced by plant breeders and pathologists. Our inv...
Wheat is a staple food of about 40% of the world population, and continuous improvement is vital to meet the increasing demands of the world population. Climate change, a serious concern of the present time, could strongly affect the wheat crop. To mitigate the climate change effects on wheat, scientists are developing wheat germplasm tolerant to t...
Development of nutrient efficient cultivars depends on effective identification and utilization of genetic variation. We characterized a set of 276 pre-breeding lines (PBLs) for several traits at different levels of nitrogen application. These PBLs originate from synthetic wheats and landraces. We witnessed significant variation in various traits a...
Seed longevity is the most important trait related to the management of gene banks because it governs the regeneration cycle of seeds. Thus, seed longevity is a quantitative trait. Prior to the discovery of molecular markers, classical genetic studies have been performed to identify the genetic determinants of this trait. Post-2000 saw the use of D...
Climate change is an undeniable threat to sustainable wheat production in the future as an increased temperature will significantly increase grain loss due to the increased number of generations per season of multivoltine species that are detrimental to plants. Among insects, orange wheat blossom midge (OWBM), yellow wheat blossom midge (YWBM), sad...
Plant genetic resources are stored and regenerated in > 1750 gene banks storing > 7,000,000 accessions. Since seeds are the primary storage units, research on seed longevity is of particular importance. Quantitative trait loci (QTL) analysis of 15 traits related to seed longevity and dormancy using 7584 high-quality SNPs recorded across 2 years and...
Fusarium head blight (FHB) of wheat, caused by Fusarium graminearum (Schwabe), is a destructive disease worldwide, reducing wheat yield and quality. To accelerate the improvement of scab tolerance in wheat, we assessed the International Triticeae Mapping Initiative mapping population (ITMI/MP) for Type I and II resistance against a wide population...
The effective utilization of natural variation has become essential in addressing the challenges that climate change and population growth pose to global food security. Currently adopted protracted approaches to introgress exotic alleles into elite cultivars need substantial transformation. Here, through a strategic three-way crossing scheme among...
Continuous increase in global population prompts increased wheat production. Future wheat breeding will heavily rely on dissecting molecular and genetic bases of wheat yield and related traits which is possible through the discovery of quantitative trait loci (QTLs) in constructed populations, such as recombinant inbred lines (RILs). Here, we prese...
The rise in human population necessitates the use of all available tools to enhance wheat productivity. In this regard, pre-breeding has mobilized novel underutilized genetic variation into breeding programs. However, this germplasm needs to be characterized for its efficient utilization. This investigation was initiated to evaluate the early and l...
Genetic mapping of seed germination traits has been performed with many plant species. In tobacco, however, investigations are rare. In the present study, a bi-parental mapping population consisting of 118 doubled haploid lines and derived from a cross between 'Beinhart-1000' and 'Hicks' was investigated. Four germination-related traits, total germ...
The rise in human population necessitates the use of all available tools to enhance wheat productivity. In this regard, pre-breeding has mobilized novel under-utilized genetic variation into breeding programs. However, this germplasm needs to be characterized for its efficient utlization. This investigation was initiated to evaluate the early and l...
Wheat is a foremost food grain of Pakistan and occupies a vital position in agricultural policies of the country. Wheat demand will be increased by 60% by 2050 which is a serious concern to meet this demand. Conventional breeding approaches are not enough to meet the demand of growing human population. It is paramount to integrate underutilized gen...
This study was initiated to identify genomic regions conferring resistance to Karnal Bunt (KB) disease in wheat through a genome-wide association study (GWAS) on a set of 179 pre-breeding lines (PBLs). A GWAS of 6,382 high-quality DArTseq SNPs revealed 15 significant SNPs (P-value <10⁻³) on chromosomes 2D, 3B, 4D and 7B that were associated with KB...
Global durum wheat consumption (Triticum durum Desf.) is ahead of its production. One reason for this is abiotic stress, e.g., drought. Breeding for resistance to drought is complicated by the lack of fast, reproducible screening techniques and the inability to routinely create defined and repeatable water stress conditions. Here, we report the fir...
Global durum wheat consumption (Triticum durum Desf.) is ahead of its production. One reason for this is abiotic stress, e.g., drought. Breeding for resistance to drought is complicated by the lack of fast, reproducible screening techniques and the inability to routinely create defined and repeatable water stress conditions. Here, we report the fir...
Worldwide, ex situ genebanks are given the task to store seeds to prevent the danger of extinction of plant genetic resources. A regular monitoring of their germination capacity is central to any genebank and any drop in that beyond a certain threshold determines their regeneration cycle. Seed longevity varies among different species and is a quant...
Worldwide, ex situ genebanks are given the task to store seeds to prevent the danger of extinction of plant genetic resources. A regular monitoring of their germination capacity is central to any genebank and any drop in that beyond a certain threshold determines their regeneration cycle. Seed longevity varies among different species and is a quant...
Frequent changes in ecosystems and environments through the change of climate have modified the rainfall patterns and seasons. This unpredictability has placed new emphasis on breeding resilient wheat varieties alongside higher yield and better nutritional quality. Use of few successful varieties as parents for breeding new varieties led to the los...
Questions
Question (1)
Dear all.
Hello. I am trying to self learn the usage of r in genetical analysis. Can anyone tell me structure of .csv file which can be read in R from where I can start learning various executions in "Genetics" package of R. A hypothetical example would be highly appreciated.
Best regards