Mhd Ikhwanuddin

Mhd Ikhwanuddin
Universiti Malaysia Terengganu | umt · Institute of Tropical Aquaculture

PhD

About

311
Publications
116,328
Reads
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2,851
Citations
Additional affiliations
January 2009 - present
Universiti Malaysia Terengganu
Position
  • Senior Researcher

Publications

Publications (311)
Article
Full-text available
Environmental factors affect the biological functions of aquaculture species both in the wild and in captivity. Therefore, optimization of the husbandry conditions under captivity can improve the production performance of an aquaculture species. In this study, the effect of water velocity on the glucose concentration, energy level of different body...
Article
The present study examined the physical characteristics of semi-moist formulated feeds to the reproductive maturation of female orange mud crab, Scylla olivacea. In this study, two experiments were conducted. The first experiment compared four types of natural diets; fish offal (discarded organ), mangrove clams (Polymesoda erosa), black devil snail...
Article
Chitin is a kind of insoluble structural polysaccharide and plays different roles in different species. In crustaceans, it forms the structural components in the exoskeleton. In our previous studies, novel mud crab hybrids have been produced from the interspecific hybridization of Scylla serrata ♀ × S. paramamosain ♂. Some of the hybrid crabs have...
Article
The influence of temperature on sex determination has been reported previously on many crustaceans. The interpretability of temperature capability to regulate the ster-oidogenic pathway, which in turn governs the offspring sex of mud crab, still remains unclear. Thus, the aim was to determine the effects of temperature on sex percentage and steroid...
Article
Full-text available
The main focus of the study was to assess the effect of lipid levels on the reproduction of the female orange mud crab, Scylla olivacea. Four isonitrogenous feed (~420 g/kg) were formulated with different lipid percentages: T1 = 60 g/kg, T2 = 80 g/kg, T3 = 100 g/kg, and T4 = 120 g/kg. A feeding trial was carried out on 120 matured female mud crabs...
Article
The rapid advancement of next‐generation sequencing technologies has promoted the use of transcriptomics in non‐model organisms, including species in the aquaculture sector. Compared to that of other crustacean species, portunid crab aquaculture is impeded by the insufficient knowledge of their biological and physiological processes. This systemati...
Article
Understanding the effects of different natural diets on the growth performance and nutritional quality of mud crab Scylla paramamosain is important for both aquaculture and human nutrition perspectives. In the present study, female S. paramamosain were fed with two different natural diets, a marine bivalve species Potamocorbula rubromuscula (initia...
Article
Study on ovarian maturation stages were carried out using external morphological assessment of ovarian colourations and histological assessment of Oocyte structure for broodstock selection purpose. Stage 1 to Stage 4 of female shrimps broodstock were sampled from Kuala Muda, Kedah, Malaysia. Four obvious colourations of ovary stages were identified...
Article
In the aquaculture of fish and crustaceans, the indoor culture of M. macrocopa constitutes a requisite to produce a more effective feed for the larviculture of the cultured commercial species. The present study examined the efficiency of different diets on population density, survival, and productivity of M. macrocopa. The cladocerans [Cladocera: c...
Article
Biofloc technology is being applied increasingly in shrimp aquaculture. Many countries have adopted this technology to increase shrimp aquaculture production. Owing to its major importance, this study was conducted to identify the bacterial population from biofloc aquaculture shrimp pond water of Singkir Laut, Kedah, Malaysia. Samples of mature bio...
Article
In Scylla mud crab farming, limb autotomy is commonly used in soft-shell crab production to expedite the moulting process and temporarily achieve high stocking density during crablet transportation. However, little is known about the effect of limb loss on the feeding ability of mud crabs. Therefore, we investigated the effect of various degrees of...
Preprint
Full-text available
Background and Objectives: Blue swimming crab, Portunus pelagicus (Linnaeus, 1758) are economically important sources of soft-shell products for export demand. However, increasing over-exploitation and pollution towards the sea resources affected the production of the P. pelagicus . The study aimed to determine the microalgal preference and feeding...
Article
To provide a valuable framework for genome sequence assembly towards the elucidation of the F1 hybrid Scylla (Scylla serrata ♀ × S. paramamosain ♂) genome as well as a reference for QTL mapping and maker-assisted selection, herein F1 hybrid offspring were obtained and used to construct the first genetic linkage map of hybrid Scylla. We presented a...
Article
Full-text available
The doublesex and mab-3 related transcription factor (DMRT) gene family is conserved from invertebrates to humans. The functions of DMRT are mainly involved in sex development and the formation of many tissues and organs. In this study, a DM (Doublesex/Mab-3)-domain gene was identified in the mud crab Scylla paramamosain, and was named SpDMRT99B be...
Article
Full-text available
Spiny lobsters of the genus Panulirus are economically important and support local fishing communities. However, mud spiny lobster Panulirus polyphagus is among the least known species within this genus in terms of their biological information. This study relates to the size distribution, length-weight relationship, and size at morphometric maturit...
Chapter
Recently, coronavirus disease 2019 (Covid-19) has posed a threat to global food security. Assessing recent literature on food security in the presence of Covid-19 is important for policymakers and funding sponsors to make future decisions. While research on the health impacts of Covid-19 is rapidly emerging, there is limited evidence on the food se...
Article
Full-text available
Understanding factors affecting the reproductive performance of mud crab (Scylla paramamosain) is important for the optimization of the culture conditions. There are some indications that complete darkness could enhance and accelerate the spawning in female decapods, but sometimes it may lead to low larval quality. Therefore, the present study was...
Article
This study focused on the effect of Noni, Morinda citrifolia fruit extract supplementation on the growth and the hepatopancreas function of Whiteleg shrimp, Penaeus vannamei Post Larvae (PL). The crude extract of M. citrifolia fruit was obtained through drying and methanol extraction followed by concentration through rotary evaporation. The extract...
Article
Interest in the production of crustacean aquaculture has focussed primarily on mud crab species; however, mud crab production still relies on wild‐caught juveniles due to the low production of viable seeds of mud crab. Thus, the aim of this study was to enhance mass propagation of purple mud crab seed by studying the relation of temperature on grow...
Article
This experiment aimed to evaluate the efficiency of formulated feeds with different lipid levels (T1 = 60 g/kg, T2 = 80 g/kg, T3 = 100 g/kg and T4 = 120 g/kg) in the biochemical composition of female orange mud crab, Scylla olivacea, during the fattening period. Experiments were conducted on 120 crabs with BW and CW of 186.42 ± 37.90 g and 10.24 ±...
Article
Brachyuran aquaculture is gaining attention due to its high market value across the globe. In efforts to maximize the benefits of monosex culture in brachyurans, sex manipulation techniques benefit from the knowledge of sex determination system. However, unlike fish, information on the sex determination of decapod crustaceans including brachyurans...
Article
Ecdysone receptor (EcR) is the primary regulator of the ecdysteroid signalling pathway, a critical pathway that directly links to moulting. In addition, EcR also regulates growth, development, reproduction and regeneration in crustaceans. However, there remains a huge gap of knowledge between the detailed structure and functional role(s) of crustac...
Article
Full-text available
Identification and exploring the role of novel sex-related genes is a crucial step for understanding the regulation mechanism of sexual development. In the present study, we identified a novel sex-related gene (designated as Sp-Pol) upstream of a sex-specific single nucleotide polymorphism (SNP). SNP1888 together with Sp-Pol were mapped on LG32 (wh...
Article
Full-text available
Sexual dimorphism is generally obvious in brachyurans, and sexual dimorphism between species is of a higher degree to allow separation of trophic niches compared to the lower intraspecific variations between sexes. Mud crab genus Scylla are distributed along the Indo-West Pacific region, and species within this genus often exhibit overlapping niche...
Article
Sexual dimorphism in mud crab occurs from the early crablet stage until the crab has the ability to mate. The development of sexual dimorphism is involved in both morphology and physiology. In terms of morphology, the mud crab expressed several characteristics such as the abdomen, chelae, size, weight and reproductive system (gonopore, gonopod...
Article
Full-text available
In order to uncover the sexual difference in morphology and how early they appear during the development stage of mud crab Scylla paramamosain, we measured, observed, and biostatistically analyzed morphological traits related to sex. For unveiling the morphological differences between sexes, morphological traits involving abdomen width (AW), carapa...
Article
The increasing demand for fish produced by aquaculture industries has led to a high demand of live feeds, such as larvae. Further development of culture techniques is important to lower the production costs as well as to maximize the production of natural food for larval rearing. Moina macrocopa is a potential freshwater live feed that can replace...
Article
Full-text available
The nursery stages of mud crab, genus Scylla, proceed from the megalopa stage to crablet instar stages. We review the definition and several of the key stages in mud crab nursery activities. The practice of the direct stocking of megalopa into ponds is not recommended due to their sensitivity. Instead, nursery rearing is needed to grow-out mud crab...
Article
Full-text available
Crabs within the family Portunidae are important marine species in both aquaculture and fishery sectors. The current aquaculture status of most portunids, however, still relies on wild-caught fisheries due to the lack of essential knowledge regarding their reproductive biology and underlying governing mechanism. With the advancement of sequencing t...
Article
Full-text available
The increasing market demand for decapods has led to a considerable interest in cultivating decapod species at a larger scale. Following the development of hatchery technologies, most research has focused on the development of formulated feeds for commercially farmed decapods once they enter the juvenile stages. The use of formulated feed for decap...
Preprint
Full-text available
Background: We previously discovered a potential sex-specific single nucleotide polymorphism (SNP) locus named SNP1888 in mud crab (Scylla paramamosain). Methods: In this study, we first verified SNP1888 to be truly a female-specific locus and then we identified a novel imprinted gene (designated as Sp-Pol) at the upstream of SNP1888 (SNP1888 is lo...
Article
The wingless-type MMTV integration site family member-4 (Wnt4), a member of the wingless-related integration site (Wnt) family, is widely accepted as a key regulator of ovarian development in mammals. In this study, a full-length cDNA of Wnt4 (designated as Sp-Wnt4) was cloned, characterized, and functionally studied in mud crab (Scylla paramamosai...
Article
Full-text available
Microbial communities in biofloc technology (BFT) are responsible in minimalize water exchange and producing microbial proteins that are beneficial as a supplementals diet for mud crab, Scylla paramamosain larvae culture. Therefore, a study was conducted to determine the effect of bacterial communities in different sedimentable solids in order to i...
Article
Full-text available
This study was carried out to determine the physiological changes (survival, growth, molting cycle, sex differentiation, and gill condition) of mud crab, Scylla paramamosain crablet at different water temperatures of 24, 28 and 32 °C, and ambient temperature of 27 to 30 °C. Thermoregulatory behavior, represented by preferred temperature (29.83 ± SD...
Article
Full-text available
Crustacean culture has been developing rapidly in various parts of the world. Therefore, it is important to understand their reproductive biology. Insulin-like androgenic gland hormone (IAG) secreted from the androgenic gland (AG) is widely accepted as a key regulator of sexual differentiation in male crustaceans. However, recently several sex-rela...
Article
Full-text available
Sexual dimorphism is a common phenomenon in the animal kingdom. To test the consistency of sexual dimorphism patterns among sympatric species of the same genus, ten morphometric characteristics of mud crabs Scylla olivacea, S. tranquebarica and S. paramamosain were measured and compared using Discriminant Function Analysis (DFA). The descriptive an...
Article
Soft‐shell crabs are gaining attention internationally as a more lucrative option of selling commercially important portunid species due to their ease of consumption, high nutritional values, and unique and excellent taste. This product, however, is only attainable in captivity as crabs are harvested right after moulting when their exoskeletons are...
Article
Full-text available
The United Nation’s 2030 development agenda adopted in 2015 outlines 17 Sustainable Development Goals (SDGs), and the organization has continued to put food security in the center of its vocalization. Aquaculture is currently the fastest-growing food production sector globally and a sustainable option for attaining food security. Food as a basic ne...
Article
Characterization of the morphometric traits of crustacean larvae is the basis for comparative works in many fields, including ecology, fisheries and aquaculture. Often, field sampled specimens will be stored in preservative before being sorted in the laboratory. Length changes are significant in fish larvae stored in different preservatives. Howeve...
Article
Multiple species of mud crabs are known to co-exist sympatrically within the same ecosystem. However, little is known about the potential factors influencing their community composition. In addition, four types of presumed hybrids (SH 1–4) of the genus Scylla with distinct variations in morphological and morphometric characters were reported recent...
Article
Full-text available
The mud crab Scylla paramamosain is a species with significant sexual dimorphism in growth rate and body size, of which the females are of higher economic and nutritional values than the males. Accordingly, there is an urgent need to explore the molecular mechanism underlying sex determination and gonadal differentiation. The single-molecule long-r...
Article
Full-text available
Malaysian aquaculture sector consists of 391,000 t of cultivated organisms produced per year with an economic value for over USD 700 million as estimated in 2019. The COVID-19 pandemic caused a large global human pandemic and it is currently ongoing, affecting profoundly Malaysian aquaculture, which is vital for seafood provision and security assur...
Article
Black tiger shrimp (Penaeus monodon) is one of the most important internationally traded fishery species, and the commercial success of their aquafarming primarily depends on the high quality broodstock for seed production. Therefore, identifications of genetically diverse broodstocks of P. monodon are essential to eliminate inbreeding effects, ens...
Preprint
Full-text available
This study attempted to explain the definition, objectives, and key stages in mud crab nursery activities. The nursery stages of Scylla spp was started from megalopa stage to several crablet instar stages or started from earlier crablet stage to several crablet stages. Direct stocking of megalopa into ponds is not recommended due to their sensitivi...
Preprint
There are several literatures that cover different views of crustacean food, feeding and behavior aspects, but little was known on its interaction between them and it's also shown a different perspective. Thus, a better understanding of the interactions between food, feeding and diets in crustaceans is vital for developing better quality of seed or...
Article
Crustaceans play essential roles as food sources for global coastal communities, and their fisheries and aquaculture support the economic growth of many coastal countries. Thus, the understanding of diseases in crustaceans, especially those with high economic values, are important for their cure and prevention. Among other parasites that parasitise...
Article
Mud crab aquaculture is gaining attention in tropical and subtropical regions for its high commercial value. Mating is the fundamental requirement for the natural seed production of mud crabs. The aim of this study was to develop an induced mating technique of orange mud crab, Scylla olivacea in captivity to support sustainable mud crab farming thr...
Article
The optimum distance and duration of ultraviolet (UV) irradiation for the complete inactivation of African catfish Clarias gariepinus egg nucleus was investigated in this study. The UV light was suspended above the unfertilized eggs at four distances (5, 10, 20 and 30 cm) and for five durations (1, 2, 3, 4 and 5 min). Then, the irradiated eggs were...
Article
Full-text available
The sustainability of aquaculture industry strongly depends on numerous factors such as environment, ecology, economics, industry, human behaviour, policy and many others. The interdependence and balance of these factors is called as eco-aquaculture. However, eco-aquaculture field has not been widely studied, especially in Malaysia. Therefore, to e...
Article
Full-text available
Infestation of Sacculina beauforti on an aquaculture species, mud crab Scylla olivacea is alarming due to its high prevalence and the extreme morphological changes in hosts. To further understand its pathological effect on growth and reproduction of S. olivacea, gonadal and hepatopancreatic histological changes of infected individuals were compared...
Article
Full-text available
Gynogenesis is an established technique to generate all female type offspring and this technique has been successfully induced diploid gynogens progeny in aquatic animals of fishes and crustaceans. Monosex culture of all female shrimp and fishes were selected attribute to all female type offspring which have better size than male and help increase...
Article
Full-text available
Background and objective: Biofloc application has been introduced in aquaculture in order to reduce the nutrients level and sustain good water quality. Due to its importance, a study was conducted to identify the effect of biofloc application on shrimp growth performance, specific growth rate and survival rate in a closed hatchery system. Materia...
Article
Full-text available
Molt stages in Giant freshwater prawn, Macrobrachium rosenbergii is among one of the most important factor affecting feed intake and their biochemical compositions. The present study was conducted to determine the effects of various molting stage on the feed intake and biochemical composition of glucose level, lipid and protein in M. rosenbergii du...
Article
Full-text available
The year 2020 was being ushered in by a global pandemic. Within months, COronaVIrus Disease (COVID-19) has spread to almost every country. With its long incubation period and highly contagious characteristics, most countries have initiated lockdown and social distancing is the new norm in most societies. Malaysia implemented the Movement Control Or...
Article
Full-text available
The present study aims to isolate, identify, and evaluate potential probiotic, Lactic Acid Bacteria (LAB) from the gut and hepatopancreas of 10 healthy wild giant freshwater prawn, Macrobrachium rosenbergii. The samples were cultivated using a modified De Man, Rogosa and Sharpe (MRS) agar supplemented with 2% of CaCO 3 (w/v). Bacterial colonies wit...
Article
Full-text available
This study aimed to determine the effect of rearing water temperature on survival, growth and molting cycle of the mud crab, S. paramamosain from Megalopa up to 1st crablet instar (Crablet 1) and from Crablet 1 to 2nd crablet instar (Crablet 2). Different water temperature regimes were implemented i.e. 20 °C, 24 °C, 28 °C, 32 °C, 36 °C and ambient...
Article
Ecdysis is a common phenomenon that happens throughout the life phase of the giant freshwater prawn Macrobrachium rosenbergii. It is vital to better understand the correlation between cannibalism and biochemical compound that exists during the moulting process. The objective of the present study was to determine the amino acid profile released by M...
Article
Full-text available
The experiment was conducted to determine the effects of different substrate on the survival, growth and total number of moulting in juvenile red claw, Cherax quadricarinatus for aquaculture purposes. In the present study, there is a problem in culturing juvenile stage of C. quadricarinatus especially for survival and growth. Thus, a substrate was...
Article
This review focuses on the potential of Moina sp. as a promising live feed in aquaculture, as well as on low-cost enrichment methods, which can improve nutrition levels available from this live feed species. Since formulated feed for this purpose has not yet been established effectively, the features of live feeds in the performance of marine and f...