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17
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February 2013 - October 2016
Publications
Publications (17)
Second‐harmonic generation (SHG) microscopy provides a high‐resolution label‐free approach for noninvasively detecting collagen organization and its pathological alterations. Up to date, several imaging analysis algorithms for extracting collagen morphological features from SHG images—such as fiber size and length, order and anisotropy—have been de...
Non‐linear microscopy is a powerful imaging tool to examine structural properties and subcellular processes of various biological samples. The competence of Third Harmonic Generation (THG) includes the label free imaging with diffraction‐limited resolution and three‐ dimensional visualization with negligible phototoxicity effects. In this study, TH...
Aging is the major risk factor for several life-threatening pathologies and impairs the function of multiple cellular compartments and organelles. Age-dependent deterioration of nuclear morphology is a common feature in evolutionarily divergent organisms. Lipid droplets have been shown to localize in most nuclear compartments, where they impinge on...
The availability of non-invasive technologies, which can be used separately or in combination for obtaining chemical composition data and structural information of Cultural Heritage (CH) materials, is of prime importance for improving the understanding the environmental or ageing impact on monuments and artefacts and defining optimal strategies for...
Polarization-dependent second harmonic generation (PSHG) microscopy is used as an innovative, high-resolution, non-destructive, and label-free diagnostic imaging tool to elucidate biological issues with high significance. In the present study, information on the structure and directionality of collagen fibers in equine pericardium tissue was collec...
The in‐vivo elucidation of the molecular mechanisms underlying muscles dysfunction due to aging via non‐invasive label free imaging techniques is an important issue with high biological significance. In the present study, polarization‐dependent second‐harmonic generation (PSHG) was used to evaluate structural alterations in the striated muscles dur...
A range of sophisticated imaging techniques have been developed in recent years that can reveal the surface structure of cultural heritage objects with varying precision. In combination with various spectroscopic methods, they allow the study of the chemical composition of the object; thus, conclusions can be drawn about the origin of the object or...
In this paper, the authors present an overview of the implementation of non-linear imaging contrast microscopy techniques as novel, powerful diagnostic tools for cultural heritage (CH) studies. These high-resolution, non-destructive modalities are employed for the in-depth compositional identification and morphological characterisation of protectiv...
This paper examines for the first time the potential complementary imaging capabilities of Optical coherence tomography (OCT) and non-linear microscopy (NLM) for multi-modal 3D examination of paintings following the successful application of OCT to the in situ, non-invasive examination of varnish and paint stratigraphy of historic paintings and the...
Age-dependent collapse of lipid homeostasis results in spillover of lipids and excessive fat deposition in non-adipose tissues. Ectopic fat contributes to lipotoxicity and has been implicated in the development of a metabolic syndrome that increases risk of age-associated diseases. However, the molecular mechanisms coupling ectopic fat accumulation...
The non-linear techniques Second and Third Harmonic Generation (SHG, THG) have been employed simultaneously to
record three dimensional (3D) imaging and localize the lipid content of the muscular areas (ectopic fat) of Caenorhabditis
elegans (C. elegans). Simultaneously, Two-Photon Fluorescence (TPEF) was used initially to localize the stained lipi...
The elucidation of the molecular mechanisms that lead to the development of metabolic syndrome, a complex of pathological conditions including type-2 diabetes, hypertension, and cardiovascular diseases, is an important issue with high biological significance and requires accurate methods capable of monitoring lipid storage distribution and dynamics...
The non-linear techniques Second and Third Harmonic Generation (SHG, THG) have been employed simultaneously to record three dimensional (3D) imaging and localize the lipid content of the muscular areas (ectopic fat) of Caenorhabditis elegans (C. elegans). Simultaneously, Two-Photon Fluorescence (TPEF) was used initially to localize the stained lipi...
The present work investigates the applicability of nonlinear imaging microscopy for the precise assessment of degradation of the outer protective layers of painted artworks as a function of depth due to aging. Two fresh and artificially aged triterpenoid varnishes, dammar and mastic, were tested. Nonlinear imaging techniques have been employed as a...
The cell membrane is a highly complex structure consisting of a large diversity of phospholipids and macromolecules. There exist a variety of diseases that compromise the integrity of this key component of the cell. This thesis considers the investigation of interactions between β-amyloid peptide (Aβ₄₂) and lipid bilayers. To facilitate understandi...
We discuss the range of tip-enhanced optical microscopies - namely mapping scattered light, fluorescence, Raman and coherent anti-Stokes Raman scattering (CARS) signals on the scale of 10-20 nm. Through the use of measurements and finite-element simulations, we explain what the optimum tip materials and sizes are and illumination wavelengths to use...
