Melina lopez-meyerInstituto Politécnico Nacional | IPN · Agricultural Biotechnology
Melina lopez-meyer
Ph.D.
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72
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1,865
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Citations since 2017
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January 2000 - June 2016
Publications
Publications (72)
La diversidad de la microbiota asociada a la rizósfera de cultivos en sistemas agrícolas ha sido pobremente estudiada en México y en todo el mundo. El objetivo de este trabajo fue identificar la diversidad de bacterias cultivables en campos de maíz. Se creó una colección de cepas de 11,520 aislados purificados a partir de la rizósfera de maíz. Se p...
Bacillus circulans E9 (now known as Niallia circulans) promotes plant growth-producing indole-3-acetic acid (IAA), showing potential for use as a biofertilizer. In this work, the use of a low-cost medium containing industrial substrates, soybean, pea flour, Solulys, Pharmamedia, yeast extract, and sodium chloride (NaCl), was evaluated as a substitu...
Stevia rebaudiana (Bertoni) Bertoni is a plant of economic interest in the food and pharmaceutical industries due its steviol glycosides (SG), which are rich in metabolites that are 300 times sweeter than sucrose. In addition, S. rebaudiana plants contain phenolic compounds and flavonoids with antioxidant activity. Endophytic bacteria promote the g...
Arbuscular mycorrhizal symbiosis is an association that provides nutritional benefits to plants. Importantly, it induces a physiological state allowing plants to respond to a subsequent pathogen attack in a more rapid and intense manner. Consequently, mycorrhiza-colonized plants become less susceptible to root and shoot pathogens. This study aimed...
α-tocopherol is found in high concentrations in avocado fruit mesocarp, however, its accumulation and genetic control during maturation and ripening has not been elucidated. Based in the relevance of VTE1 and VTE5 genes in tocopherol biosynthesis and aiming to determine the association between tocopherol accumulation and expression of tocopherol bi...
Randia echinocarpa (Rubiaceae) is a plant native to Mexico that extends along the Pacific coast and is used in traditional Mexican medicine. Previous studies have shown that the fruit of R. echinocarpa presents high antioxidant, antimutagenic, and antidiabetic activities, and was innocuous in toxicity tests in mice. Unfortunately, wild populations...
Arbuscular mycorrhizal fungi (AMF) have the potential to reduce the deleterious effect of soil-borne pathogens, but their ability for pathogen biocontrol may vary depending on the genotype of the plant, the pathogen, and the AMF interaction. Thus, the aim of this work was to evaluate the effect of the Mexican biofertilizer Rizofermic-UV based on a...
ABSTRACT
Background. After its transfaunation from the Atlantic Ocean has been reported, the protozoan Perkinsus
marinus has expanded its list of hosts in several species of mollusks in the Pacific Ocean, both in cultivation
and in wild populations. Goals. The presence of this parasite was evaluated in the horsemussel Modiolus
capax, in the sout...
The phytopathogenic fungus Macrophomina phaseolina is the causal agent of charcoal rot of common beans, in which it damages the roots, stems and leaves of seedlings and plants. Due to the ability of the fungus to form resistant structures, chemical control is not sufficient for disease control. Integrated management systems associated with the use...
Antecedentes:
La defensa inducida por micorrizas se ha reportado ampliamente, en la cual se ha sugerido la participación de la ruta de señalización de las oxilipinas, principalmente el ácido jasmónico; la participación de su derivado metil-jasmonato (MeJA) es controversial.
Objetivos:
Evaluar el efecto de la colonización con Rhizophagus irregulari...
Stevia rebaudiana is an economically important crop species, due to its ability to produce steviol glycosides, which are compounds that are 300 times sweeter than sugar. Additionally, this species produces phenolic compounds and flavonoids, a portion of which accumulate in trichomes. Arbuscular mycorrhizal (AM) symbiosis is the most important mutua...
Detección de Perkinsus marinus en una población silvestre del mejillón "choro" Modiolus capax del suroeste del Golfo de California Detection of Perkinsus marinus in a wild population of the horsemussel Modiolus capax from the southwestern Gulf of California
Sugars act not only as substrates for plant metabolism, but also have a pivotal role in signaling pathways. Glucose signaling has been widely studied in the vascular plant Arabidopsis thaliana, but it has remained unexplored in non-vascular species such as Physcomitrella patens. To investigate P. patens response to high glucose treatment, we explor...
Background
Common bean ( Phaseolus vulgaris L. ) is a relevant crop cultivated over the world, largely in water insufficiency vulnerable areas. Since drought is the main environmental factor restraining worldwide crop production, efforts have been invested to amend drought tolerance in commercial common bean varieties. However, scarce molecular dat...
In plants, phosphorus (P) uptake occurs via arbuscular mycorrhizal (AM) symbiosis and through plant roots. The phosphate concentration is known to affect colonization by AM fungi, and the effect depends on the plant species. Stevia rebaudiana plants are valuable sources of sweetener compounds called steviol glycosides (SGs), and the principal compo...
The protozoan Perkinsus marinus has been associated with high mortality episodes of the eastern oyster Crassostrea virginica in the USA. The presence of P. marinus on the pleasure oyster Crassostrea corteziensis cultivated in two estuaries on the southeast coast of the Gulf of California was evaluated. Oysters were collected monthly (September 2016...
Dry common beans are important food crops in Mexico, and represent a significant
source of carbohydrates, proteins, lipids, minerals, and phenolic compounds in the
Mexican diet. Phenolic compounds are important mainly due to their antioxidant
properties, and their content differs among common bean varieties. It has been reported
that arbuscular myc...
Anthocyanins are colored water-soluble pigments found widespread in plants, which are strongly accumulated during development and under biotic and abiotic stress favoring plant adaptation. Bell pepper (Capsicum annuum L.) exhibits anthocyanin pigmentation during development and abiotic stress conditions, making it an interesting model to study anth...
Prevalencia de Perkinsus sp. (Apicomplexa) en la almeja chocolata Megapitaria squalida de la costa central de Sinaloa, México Prevalence of Perkinsus sp. (Apicomplexa) in the callista clam Megapitaria squalida from the central coast of Sinaloa, Mexico-Ulloa. 2019. Prevalencia de Perkinsus sp. (Apicomplexa) en la almeja chocolata Megapitaria squalid...
The objective of this study was to evaluate the effect of four exogenous phosphate concentrations on AM symbiosis establishment, and theirs relation on photosynthetic performance
Dry common beans are important food crops in Mexico, and represent a significant source of carbohydrates, proteins, lipids, minerals, and phenolic compounds in the Mexican diet. Phenolic compounds are important mainly due to their antioxidant properties, and their content differs among common bean varieties. It has been reported that arbuscular myc...
Stevia rebaudiana is a perennial herb of significant economic value due its the high content of steviol glycosides (SG´s), natural sweeteners found in leaves. Arbuscular mycorrhizal (AM) symbiosis is a biotechnological strategy used in agriculture to reduce the use of agrochemical and may increase mineral nutrients, uptaker water, plant growth, bio...
Arbuscular mycorrhizal (AM) symbiosis may uptake water, increase phosphate translocation and induce changes at the photochemical level. It is known that phosphate concentration plays an important role in symbiotic and nutritional development. Additionally, AM symbiosis can generate biotic stress, modifying its photochemical activity [1]. Stevia reb...
Heterotrimeric G-proteins (G proteins) constitute a classical signal transduction system which can operate together with G-protein coupled receptors (GPCRs) and regulators of G-protein signaling (RGS) in environmental stress-related pathways. Among several environmental factors that can cause stress in plants, ultraviolet B (UV-B) radiation affects...
A comparative proteomic approach was performed to analyze the differential accumulation of leaf proteins in response to the symbiosis between Solanum lycopersicum and the arbuscular mycorrhizal fungus (AMF) Rhizophagus irregularis. Protein profiling was examined in leaves from tomato plants colonized with AMF (M), as well as non-colonized plants fe...
Las proteínas G perciben el ambiente extracelular a través de receptores en la membrana plasmática transmitiendo señales hacia moléculas de señalización en el interior de las células conocidas como efectores. En las plantas, estos efectores comprenden algunas proteínas reguladoras de la transcripción, enzimas metabólicas, fosfolipasas y proteínas d...
Low temperature (LT) and ultraviolet-B (UV-B) radiation are abiotic factors that cause plant stress and limit crop production because of their detrimental effects on photosynthetic components. There is evidence of chlorophyll and carotenoid degradation by LT and UV-B radiation, as well as the production of phenolic compounds as defense components a...
Species in the fungal genus Trichoderma have been reported to modulate tolerance to heavy metal stress in plants. However, the mechanism for enhanced plant tolerance to copper (Cu) by Trichoderma spp., has received little attention. To evaluate the potential of the fungus Trichoderma asperellum (To strain) to reduce the adverse effects of Cu on pla...
Cellulolytic activities in Bacillus subtilis have been demonstrated and it is known that the eglS gene encodes an endoglucanase that could play a key role. Three Bacillus subtilis strains (RZ164, RS351, and RS273) isolated from corn stover with contrasting cellulase activity were examined in this work. The aim was to analyze the influence of eglS g...
G-proteins perceive the extracellular environment through receptors on the plasma membrane and transmit signals to signaling molecules inside the cells known as effectors. In plants, these effectors comprise some transcription regulatory proteins, metabolic enzymes, phospholipases and scaffold proteins of the MAPK pathway. G proteins in plants have...
Plants of tropical and subtropical origin are damaged by temperatures below 15°C; these conditions produce cold stress in plants. The degradation of the ozone layer leads to a greater penetration of ultraviolet-B (UV-B) radiation to the planet's surface, especially in tropical and subtropical latitudes, which interferes with plant development. Cold...
The stalk, ear and root rot (SERR) of maize caused by Fusarium verticillioides (Fv) severely impacts crop production in tropical and subtropical regions. The aim of the present work was to screen bacterial isolates in order to find novel native biocontrol agents against Fv. A culturable bacterial collection consisting of 11,520 isolates enriched in...
The effect of mycorrhizal colonization on reducing susceptibility to Sclerotinia sclerotiorum was investigated in four common bean genotypes: Azufrado Regional 87 (Az Reg 87), Azufrado Higuera (Az Hig), A-55, and BAT 93 using the 'straw test' method. Colonization by the arbuscular mycorrhizal fungi Rhizophagus irregularis (formerly known as Glomus...
Pathogenicity of microencapsulated spores of Beauveria bassiana (Balsamo) Vuillemin and Metarhizium anisopliae (Metsch) Sorokin at a dose of 1 × 10 8 spores per milliliter were used against tobacco budworm, Heliothis virescens (Fabricius) (Lepidoptera: Noctuidae). Toxicity of non-microencapsulated spores at the same dose was also assessed. Bovine g...
Propagules of Metarhizium anisopliae (Metchnikoff) Sorokin were obtained from solid fermentation of rice grains to produce microcapsules by the spray drying technique. Bovine gelatin, nixtamalized corn, and corn starch were used as matrix, in addition to a feeding stimulant and a UV protector. Eight microencapsulated formulations were obtained adju...
In addition to the nutrient exchange that is promoted by the arbuscular mycorrhiza symbiosis (AMS) between plants and fungi, AMS triggers mycorrhiza-induced protection against plant pathogens. Although the induction of this protection against diverse plant pathogens has been described for several plant species, it is not clear if its onset differs...
Arbuscular mycorrhizal symbiosis is a beneficial association between plant roots and fungi that occurs in approximately 80 % of terrestrial plants and which confers different benefits including mineral nutrient acquisition and enhanced defense capacity. Although mycorrhizal colonization takes place in roots, the symbiosis establishment has systemic...
The arbuscular mycorrhizal (AM) symbiosis is an intimate association between specific soil-borne fungi and
the roots of most land plants. AM colonisation elicits an enhanced defence resistance against pathogens, known as mycorrhizal-induced resistance (MIR). This mechanism locally and systemically sensitises plant tissues to boost their basal defen...
Photosynthetic and respiratory responses (P–E curves) of Gracilaria parvispora from the southeast Gulf of California were studied at four temperatures (20, 25, 30, 35 °C) and salinity (25, 30, 35, 40 psu) combinations. The alga showed acclimation in its photosynthetic and respiratory responses to tropical temperature as well as to oceanic salinity....
Gracilaria parvispora is reported for the first time as an invasive species in northwestern Mexico based on morphological, anatomical and molecular data. The species is widely distributed in the region, where it colonizes sandy and rocky habitats from intertidal areas down to a depth of 1.5 m. The morphological and anatomical data from the Mexican...
Abstract.
A simplified protocol to obtain transgenic tomato plants was established. The effects of culture media composition and Agrobacterium concentration were evaluated. The highest shoot-forming capacity index (5.6) was observed when leaf explants were cultured for 6 weeks with 2 mgL–1 zeatin, 0.1 mgL–1 indoleacetic acid, and 300 mgL–1 timentin...
A simplified protocol to obtain transgenic tomato plants was established. The effects of culture media composition and Agrobacterium concentration were evaluated. The highest shoot-forming capacity index (5.6) was observed when leaf explants were cultured for 6 weeks with 2 mg·L -1 zeatin, 0.1 mg·L -11 indoleacetic acid, and 300 mg·L -1 timentin. S...
We utilized the two-compartment system to study the effect of arsenic (As) on the expression of the Glomus intraradices high-affinity phosphate transporter GiPT, and the GiArsA gene, a novel protein with a possible putative role as part of an arsenite efflux pump and similar to ArsA ATPase. Our results show that induction of GiPT expression correla...
Textbook of Molecular Biotechnology covers an amazing range of topics from the basic structure of the cell and diversity of microorganisms to the latest techniques in the field of biotechnology. Various topics have been included for the benefit of graduate and postgraduate students. In addition, the book will be of immense help for the researchers...
Plant establishment, presence of arbuscular mycorrhizal fungi (AMF) and other rhizospheric fungi were studied in mine wastes from Zimapan, Hidalgo state, Mexico, using a holistic approach. Two long-term afforested and three non-afforested mine tailings were included in this research. Fifty-six plant species belonging to 29 families were successfull...
The protective effect of a probiotic mixture (PM) and antiviral plants, against the white spot syndrome virus (WSSV) in Litopenaeus vannamei, was evaluated in three experiments. The PM was composed of four lactic acid bacteria (LAB) and one yeast strain. The plant mixture was composed of Ocimum sanctum and commercial antiviral plants (VPH®, HSV®)....
Introducción. El tomate (Solanum lycopersicum) es uno de los principales cultivos alimentarios a nivel mundial, representando una fuente importante de licopeno, vitamina C y β-caroteno. Dentro de los numerosos genotipos desarrollados de este cultivo, el cultivar Micro-Tom, ha recibido recientemente especial interés debido a sus características únic...
The plant hormone ethylene negatively regulates bacterial infection and nodule formation in legumes in response to symbiotic rhizobia, but the molecular mechanism(s) of ethylene action in symbiosis remain obscure. We have identified and characterized multiple mutant alleles of the MtSkl1 gene, which controls both ethylene sensitivity and nodule num...
In natural ecosystems, the roots of many plants exist in association with arbuscular mycorrhizal (AM) fungi, and the resulting symbiosis has profound effects on the plant. The most frequently documented response is an increase in phosphorus nutrition; however, other effects have been noted, including increased resistance to abiotic and biotic stres...
The majority of the vascular flowering plants have the ability to enter into symbiotic associations with a unique group of soil fungi, the arbuscular mycorrhizal (AM) fungi. The symbiosis develops in the roots of the plant, where the plant provides the fungus with a source of carbon and the fungus delivers mineral nutrients to the roots. In particu...
WSSV has caused great losses to the global shrimp industry in recent years. This virus can infect shrimps asymptomatically. However, once the clinical signs are developed, mortalities can reach 100% in 3–10 days. PCR has been extensively used to detect WSSV in a specific and sensitive manner. Nested PCR is even more sensitive than single-step PCR a...
Vestitone reductase (VR; E.C. 1.1.1._) and isoflavone reductase (IFR; E.C. 1.3.1.45) are phytoalexin-specific enzymes involved in the biosynthesis of medicarpin in alfalfa (Medicago sativa L.). Immunoblot analysis of total soluble protein extracts indicated that these proteins constitutively accumulate in roots and nodules of alfalfa plants, and in...
Medicarpin and maackiain are antifungal pterocarpan phytoalexins produced by many legumes, and are thought to be important components of the defense response of these legumes to certain fungal pathogens. The Mak1 gene from the fungal pathogen Nectria haematococca encodes an FAD-dependent mono-oxygenase, known to specifically hydroxylate the phytoal...
Camptothecin (CPT) is a valuable anti-cancer monoterpene alkaloid produced by the Chinese tree Camptotheca acuminata. Tryptophan decarboxylase (TDC) supplies tryptamine for the indole moiety of CPT and its derivatives, and is considered a key step in monoterpene indole alkaloid biosynthesis as it links primary and secondary metabolism. This report...
Over a 12-week period, new growth was collected at different intervals from Camptotheca acuminata trees to determine whether a leaf harvest strategy would be an efficient means for the production of the alkaloid camptothecin. Because camptothecin accumulates in young leaves and because the harvesting of young tissue stimulates axillary bud outgrowt...
An aromatic amino acid decarboxylase DNA fragment was generated from opium poppy (Papaver somniferum L.) genomic DNA by the PCR using primers designed from conserved amino acid sequences of other aromatic amino acid decarboxylase genes. Using this fragment as a probe, a genomic clone was isolated that encodes a new member of the opium poppy tyrosin...
Camptothecin is an anticancer and anti-viral alkaloid produced by the Chinese tree Camptotheca acuminata (Nyssaceae). Despite previous reports of low levels of anticancer activity in leaves of Camptotheca acuminata, we have discovered that camptothecin accumulates to approximately 0.4% of the dry weight of young leaves. This level is 1.5-fold highe...
HMGR (3-hydroxy-3-methylglutaryl-coenzyme A reductase; E.C.1.1.1.34) supplies mevalonate for the synthesis of many plant primary and secondary metabolites, including the terpenoid component of indole alkaloids. Suspension cultures of Camptotheca acuminata and Catharanthus roseus, two species valued for their anticancer indole alkaloids, were treate...
The release of alkaloids from root culturesDatura stramonium andCatharanthus roseus and thiophenes from root cultures ofTagetes patula was found to increase when the pH of the culture media (ranging from 4.8 to 7.0) was reduced to 3.5. The extent of the effect
was different in each type of culture. Increases ranged from 4- to 20-fold, which in some...
Projects
Projects (2)
The main focus is to study genes related to cell wall synthesis regulated by arbuscular mycorrhizal symbiosis. It is well known that the arbuscular mycorrhizal symbiosis induces a local response (in the root) that can trigger a systemic response in the leaves, inducing a defense response against the attack of pathogens. In particular, this research study genes involved in the synthesis of xyloglucan, the main component of hemicellulose.