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Melanie Kalischuk

Melanie Kalischuk
Aerobio Solutions Ltd. · Plant Science

PhD

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29
Publications
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Publications

Publications (29)
Article
Full-text available
Rubus yellow net virus (RYNV) was cloned and sequenced from a red raspberry (Rubus idaeus L.) plant exhibiting symptoms of mosaic and mottling in the leaves. Its genomic sequence indicates that it is a distinct member of the genus Badnavirus, with 7932bp and seven ORFs, the first three corresponding in size and location to the ORFs found in the typ...
Article
Full-text available
Abiotic and biotic stresses alter genome stability and physiology of plants. Under some stressful situations, a state of stress tolerance can be passed on to the offspring rendering them more suitable to stressful events than their parents. In plants, the exploration of transgenerational response has remained exclusive to model species, such as Ara...
Article
Full-text available
Immunity cell-surface receptors Ve1 and Ve2 protect against fungi of the genus Verticillium causing early dying, a worldwide disease in many crops. Characterization of microbe-associated molecular pattern immunity receptors has advanced our understanding of disease resistance but signal amplification remains elusive. Here, we report that transgenic...
Article
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Summer squash (Cucurbita pepo L.) is a major vegetable crop produced in Georgia and Florida during the fall season. This production is vulnerable to whitefly (Bemisisia tabaci Genn.)-transmitted viruses that lead to severe yield losses. Over the past several years, whitefly populations have increased during the fall, thus leading to an increase in...
Article
Cucurbit leaf crumple virus (CuLCrV), a single-stranded DNA virus belonging to the species Cucurbit leaf crumple virus in the genus Begomovirus, causes cucurbit leaf crumple disease in many species of the Cucurbitaceae family. This report describes the development and standardization of field-based isothermal recombinase polymerase amplification (R...
Article
Aspen and poplar trees are important horticultural plants grown in Canada for aesthetic, commercial woodlot and windbreak applications. Bronze leaf is a destructive disease in Populus spp. and is caused by the fungal pathogen Apioplagiostoma populi Barr. This pathogen is often difficult to isolate and confirm from infected plant tissues and has bee...
Article
Rose rosette disease (RRD) caused by rose rosette emaravirus (RRV) is a major issue in the U.S. rose industry with no effective method for its management. This study evaluated the effect of foliar application of Acibenzolar S-methyl (ASM), a plant systemic acquired resistance inducer in reducing RRD disease severity on Rosa species cv. Radtkopink (...
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Full-text available
Pectobacterium atrosepticum is a pectolytic enterobacterium that causes blackleg in plants. Bacteriophage Peat2 was isolated from P. atrosepticum infected potato tissue and its genome sequenced. Analysis of the 48,659 bp genome revealed that Peat2 resembles the Myoviridae family Klebsiella pneumoniae bacteriophage JD001 and includes a CRISPR Cas 4...
Article
Multispectral imaging is increasingly used in specialty crops, but its benefits in assessment of disease severity and improvements in conventional scouting practice are unknown. Multispectral imaging was conducted using an unmanned aerial vehicle (UAV), and data were analyzed for five flights from Florida and Georgia commercial watermelon fields in...
Article
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The plant viral family Luteoviridae is divided into three genera: Luteovirus, Polerovirus and Enamovirus. Without assistance from another virus, members of the family are confined to the cells of the host plant’s vascular system. The first open reading frame (ORF) of poleroviruses and enamoviruses encodes P0 proteins which act as silencing suppress...
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El tizón tardío, causado por el oomiceto Phytophthora infestans (Mont.) de Bary, es una enfermedad devastadora en papa y tomate, y causa pérdidas en rendimiento y calidad en todo el mundo. La enfermedad surgió primero en América Central y desde entonces se ha dispersado en Norteamérica, incluyendo los Estados Unidos y Canadá. Recientemente han emer...
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Potato mop-top virus (PMTV) is a member of the genus Pomovirus and has a single-stranded positive-sense RNA genome divided into three segments. PMTV is transmitted by the soilborne powdery scab pathogen, Spongospora subterranea (Wallroth) Lagerheim, which is widespread and occurs in most major potato growing areas. Ten tubers of potato (Solanum tub...
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Full-text available
Pectobacterium atrosepticum is a common phytopathogen causing significant economic losses worldwide. To develop a biocontrol strategy for this blackleg pathogen of solanaceous plants, P. atrosepticum bacteriophage Peat1 was isolated and its genome completely sequenced. Interestingly, morphological and sequence analyses of the 45,633-bp genome revea...
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The late blight pathogen (Phytophthora infestans) continues to cause major losses on potato and tomato in Canada and worldwide. An increased diversity of P. infestans and dramatic shifts in pathogen populations have occurred in Canada in recent years. In 2011, a survey identified different genotypes of P. infestans in Canada, including the new US-2...
Article
Full-text available
Late blight, caused by Phytophthora infestans (Mont.) de Bary, is the most historically significant and economically destructive disease of potatoes (Solanum tuberosum L.). In addition to potato, P. infestans can also infect tomato and some other members of the Solanaceae, and this has contributed to the recent late blight epidemic in Canada and th...
Article
Full-text available
Late blight, caused by Phytophthora infestans, is a devastating disease of potatoes and tomatoes that causes significant crop losses worldwide. Late blight diversity has been increasing since 2009 in many areas of Canada on both potatoes and tomatoes. Although the US-8 genotype of P. infestans has dominated pathogen populations in much of Canada fo...
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Potato leafroll virus (PLRV) is a positive-strand RNA virus that generates subgenomic RNAs (sgRNA) for expression of 3' proximal genes. Small RNA (sRNA) sequencing and mapping of the PLRV-derived sRNAs revealed coverage of the entire viral genome with the exception of four distinctive gaps. Remarkably, these gaps mapped to areas of PLRV genome with...
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A dramatic increase in the incidence of late blight and changes within populations of Phytophthora infestans have been observed in various regions of Canada. In this study, the occurrence of several new genotypes of the pathogen was documented with associated phenotypes that dominated pathogen populations. Genotype US-23, previously detected only a...
Article
Full-text available
Forisomes are protein polymers found in leguminous plants that have the remarkable ability to undergo reversible "muscle-like" contractions in the presence of divalent cations and in extreme pH environments. To gain insight into the molecular basis of forisome structure and assembly, we used confocal laser scanning microscopy to monitor the assembl...
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Full-text available
Bacterial ring rot (BRR) is a regulated disease of potato caused by the gram-positive bacterium Clavibacter michiganensis subsp. sepedonicus. Many countries have seed certification programs that involve pre- and post-harvest inspections for disease symptoms supplemented by laboratory immunological and nucleic acid diagnostics. Several studies have...
Article
Full-text available
Our previous experiments showed that infection of tobacco (Nicotiana tabacum) plants with Tobacco mosaic virus (TMV) leads to an increase in homologous recombination frequency (HRF). The progeny of infected plants also had an increased rate of rearrangements in resistance gene-like loci. Here, we report that tobacco plants infected with TMV exhibit...
Article
Poplar (Populus spp.) is an important ornamental, windbreak, and pulp and wood product tree in Alberta and across western Canada because of its rapid growth, architecture, and hardiness. It is also a major component of native tree stands in the parkland area of the Canadian Prairies. Until recently in North America, infections of Apioplagiostoma po...
Article
In North America, Rubus yellow net virus (RYNV), a member of the genus Badnavirus, family Caulimoviridae, is vectored in a semipersistent manner by the large raspberry aphid (Amphorophora agathonica Hottes) and is responsible for producing net-like chlorosis of tissue along the leaf veins (2). Red raspberry (Rubus idaeus L.) is commonly grown in Ca...
Article
One of the challenges of managing spruce-aspen mixedwoods in the boreal forest is the difficulty in determining site index for spruce in those stands in which it occurs primarily in the understory. A study was conducted to determine if growth increment of understory spruce, adjusted for available light, could predict spruce site index. In each of n...

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