Mehdi Soltani

Murdoch University · Fish Health

Essential oil composition of Otostegia pérsica Boiss. (Lamiaceae) from Iran was obtained by hydrodistallation and analyzed by GC and GC/MS techniques. Forty-four components representing 89.3% of the total oil were identified. The main components of the oil were dillapiole (43.1%), trans-verbenol (9.6%) and hexadecanoic acid (5.7%).

The essential oil of Tanacetum polycephalum Schultz Bip. subsp. farsicum Podl. growing wild in Iran was obtained by hydrodistallation and analyzed by GC and GC/MS. Thirty-eight components representing 84.5% of the total oil were identified. The main components of the oil were trans-chrysanthenyl acetate (24.7%), 1,8-cineole (9.2%), trans-chrysanthe...

The plant world represents an enormous reservoir of biologically active molecules. Essential oils, the odorous principles of plants, are widely employed because of their commercial uses and medicinal properties. Extensive application of essential oils for their antimicrobial activity in food preservations, pharmaceuticals, alternative medicine, and...

Composition of the essential oil oï Biebersteinia multifida DC. (Biebersteiniaceae) was investigated hy GC and GC/MS. Forty-four compounds represent 96.9% of the total oil were characterized. The main components of the oil were (E)-nerolidol (33.8%), phytol (17.7%), 6,10,14-tdmethyl-2-pentadecanone (15.5%) and hexadecanoic acid (11.2%).

The essential uil uf Ot(!stel!.ia midwl.txii Bri tl' growing wild in the Pars pruvince of Irall was uDt,(ined by hytirudis-tallation and an alyzed bv GC and GC/ :YIS techniques. Forty-Ave co mponents represl'nting 97.9"7,) of tlll> totil oi l were identified. The l1uin components of the oil we re dillapiole (23.9%), 2-methylhenzof1.lran (12.9%) and...

The essential oil of Dicyclophora persica Boiss. growing wild in Iran was obtained by hydrodistallation and analyzed by GC and GC/MS technique. Fifty-four components representing 89.0% of the total oil were identified. The main components of the oil were ρ-cymene (13.6%), α-pinene (13.4%), α−phellandrene (9.4%) and (Z)-falcarinol (5.0%).

The essential oil of Pycnocycla nodiflora Decne. ex Boiss. growing wild in Iran was obtained by hydrodistallation and analyzed by GC and GC/MS technique. Seventy-two components representing 86.8% of the total oil were identified. The main components of the oil were β-eudesmol (34.3%), hexadecanoic acid (11.5%) and spathulenol (6.9%). The genus Pycn...

The essential oils of the aerial parts of Helichrysum leucocephalum Boiss. and Helichrysum artemisioides Boiss. et Hausskn. collected from Iran were examined by GC and GC/MS. Ninety-two components of H. leucocephalum and 48 of H. artemisioides representing 93.0% and 92.4% of the oils, respectively were identified. Rosifoliol (22.3%), β-caryophyllen...

The essential oils of Eremostachys adenantha and Eremostachys macrophylla have been investigated by GC and GC/MS. Seventy-six compounds of E. adenantha and thirty-five of E. macrophylla representing 91.2% and 92.9% of the oils were identified, respectively. The main constituents of the oil of E. adenantha were dodecanal (12.7%), hexa-decanoic acid...

Essential oil composition of two species of Phlomis L. (Phlomis aucheri Boiss. and Phlomis elliptica Benth.) (Lamiaceae) from Iran Abstract The essential oils of Phlomis aucheri and Phlomis elliptica have been investigated by GC and GC/MS. Thirty-nine compounds of Ph. aucheri and fifty-eight of Ph. elliptica representing 91.2% and 90.1% of the oil...

The outer membrane protein of Aeromonas hydrophila is a potential candidate for vaccine development. In this study, after cloning and expression of ompTS, 270 common carp, weighing 44 ± 5.7 g divided into five groups, were injected intraperitoneally twice with 3‐week intervals. Groups included the following: PBS, PBS plus Freund's adjuvant, recombi...

This study was conducted to evaluate the antifungal effects of garlic (Allium sativum) and wormwood (Artemisia sieberi) ethanolic extracts on survival of rainbow trout (Oncorhynchus mykiss) eggs during the incubation and larval stages. The eggs were exposed, in triplicate, to three different doses of garlic (50, 100, 200 ppm) and wormwood (25, 50,...

The individual and combinational effects of probiotics on Persian sturgeon (Acipenser persicus) were evaluated through assessing the growth performance, proximate body composition, digestive enzymes and intestinal morphology. On the basis of feeding with experimental diets, the fish were assigned into four groups denoted as follows: control (basal...

This study aims to evaluate the effects of Bifidobacterium animalis PTCC-1631 and Bifidobacterium lactis PTCC-1736 on growth, nutrient digestibility, carcass composition, and intestinal microbiota of Oncorhynchus mykiss fry, which had initial body weights of 0.58 ± 0.19 g. Four iso-nitrogenous diets with probiotics, namely T1 (1 × 10⁷ CFU g⁻¹), T2...

Nowadays, use of the natural plant derivatives as an immunostimulant in aquaculture is becoming more useful than antibiotics which are destructive to target animals, consumers, and the environment. The present study aimed to evaluate the efficacy of dietary substitution of wheat flour at 2, 5, 7.5, and 10% of olive pomace (OP2, OP5, OP7.5, and OP10...

This study investigated the effects of nonylphenol (NP) on hematological and immunological parameters in both male and female rainbow trout (Oncorhynchus mykiss). Fish were randomly distributed into six groups and administered with NP (10, 50 and 100 μg g⁻¹ week⁻¹ BW) and a single dose of 17-β estradiol (E2; 2 μg g⁻¹ week⁻¹ BW, positive control). T...

Immunological methods (e.g. FAT, IHC, ELISA) enable the rapid specific detection of fish pathogens. Enzyme-Linked Immunosorbent Assay (ELISA) method can also be used in serology (detection of pathogen-specific antibodies in the host fishes) as a means of disease surveillance programs in aquaculture. IHN, IPN and VHS are serious viral diseases in ra...

The present study evaluated the effect of Lactobacillus plantarum as a probiotic on the growth performance and haematological parameters of juvenile rainbow trout (Oncorhynchus mykiss) weighing 29.6±1.8 g immunized with streptococcus/lactococcus bivalent vaccine for 60 days at 16±1.5ºC. Fish were randomly allocated to 4 equal groups: probiotic (g k...

The zoonotic bacteria Streptococcus iniae is the causative agent of streptococcosis associated with serious economic losses in the world aquaculture industry, including Iran. The antimicrobial activities of Thymus vulgaris, Carum carvi, and Menthalong flora essential oils were determined against two pathogenic isolates of S. iniae using broth micro...

The objective of this study was to assess the effects of rearing density on the growth and fatty acid profile of Nereis diversicolor and on its capability to bioremediate wastewater in an integrated culture system with rainbow trout (Oncorhynchus mykiss). To this end, a batch of juvenile N. diversicolor (0.03 ± 0.01 mg) was assigned into four diffe...

Replacement of olive pomace (OP) with wheat flour in diet was studied in diet of yearling Siberian sturgeon (Acipenser baerii). Fish (165 ± 6.8 g) were randomly allocated to 15 fibreglass tanks (15 fish/tank, in triplicate). Fish were assigned to one of five dietary treatments at 19.1 ± 1.5°C for 8 weeks: control diet (crude protein and crude lipid...

Adjuvant effect of G2 (extraction of buffalo spleen) was assessed in intraperitoneally immunized rainbow trout (100-150 g) with killed- Y. ruckeri bacterin biotype I [0.1 mL (1 × 107 cells/fish) of vaccine diluted with the adjuvant in a ratio of 1:1 (V/V)] at 12.4 ± 1.3 °C for 10 weeks. Leukocyte and lymphocyte counts, anti-Y. ruckeri antibody tite...

BACKGROUND: Yersiniosis or enteric redmouth disease (ERM), caused by Yersinia ruckeri, is a serious bacterial disease in the farmed salmonids that causes economic problems in this industry. OBJECTIVES: This study was aimed to assess the experimental pathogenicity of Yersinia ruckeri in Rainbow trout (Oncorhynchus mykiss). METHODS: Two hundred Rainb...

This study aimed to assess efficacy of commercial and indigenous sources of Bacillus subtilis and Bacillus lichenformis as probiotic dietary supplementation on the growth performance, immuno-physiological variables and resistance response to some stressors in white leg shrimp (Litopenaeus vannamei) weighing 50 ± 6.54 mg at 28 °C for 60 days. Shrimp...

Abdominal distention occurred at an incidence of 1% (15 from 1500 fish) in the population of 1-year-old bester (Huso huso 9 Acipenser ruthenus). Computed tomography (CT) images and radiographs showed a soft tissue mass compressed the posterior part of the swim bladder. Ultrasonography showed that the masses had different patterns. Internal examinat...

Increasing of anthropogenic electromagnetic fields in aquatic environments has been recently become the core of attention. In this study, the effect of extremely low-frequency electromagnetic fields (50 Hz) on immune status and metabolic markers of common carp fingerling was assessed. The fish were exposed to extremely low-frequency electromagnetic...

This study conducted to evaluate the effect of dietary zinc (Zn) levels on feed utilization, tissue Zn composition and serum enzyme activities of juvenile Siberian sturgeon, Acipenser baerii. Five isoenergetic and isonitrogenous semi-purified diets were formulated with increasing Zn sulphate (ZnSO4.5H2O) level to provide the actual Zn values of 14....

In this study, effects of Lactobacillus plantarum on growth performance, proteolytic enzymes activity and intestine morphology of Oncorhynchus mykiss were studied. Juvenile rainbow trout (n=270) in 3 administration groups in 9 cement tanks were kept and fed with diets containing different doses (0, 0.3 and 0.5 g kg⁻¹ feed) of the probiotic for 90 d...

Lactococcosis has been defined as acute septicaemia, which causes economic losses in farmed fish, especially in rainbow trout. This study was done to evaluate the effects of the essential oils of Satureja bachtiarica and Nigella sativa on the efficacy of lactococcosis vaccine in rainbow trout. A total number of 270 fishes with a mean weight of 120...

White spot syndrome virus (WSSV) is a double-stranded DNA virus that causes the most serious losses to shrimp farmers worldwide. Since the first WSSV incidence in Iran in 2001, it has caused several outbreaks of disease with high mortality and economic loss in the Iranian shrimp farming industry. During this study from May 2010 to November 2015, WS...

This study evaluated the effects of diets containing 0, 2.5, 5, 7.5 and 10% Arthrospira platensis on skin pigmentation, growth performance, and digestive enzymes of koi, Cyprinus carpio. A completely randomized design was performed with five treatments and three replicates. One hundred and fifty koi fish with average initial weight of 30±1g were as...

Date palm seed (Phoenix dactylifera) (DPS) extract has been used worldwide in pharmaceutical agent due to its constituents. This study aimed to assess the effects of DPS extract as a dietary supplementation at on the body composition, lipid peroxidation and tissue quality of common carp (Cyprinus carpio) based on the total volatile nitrogen (TVN) t...

IPNV in Atlantic salmon is represented by various strains with different virulence and immunogenicity linked to various motifs of the VP2 capsid. IPNV variant with P217, T221, A247 (PTA) motif is found to be avirulent in Atlantic salmon, but virulent in rainbow trout, and other salmonid species. This study describes a DNA vaccine delivered intramus...

Nowadays, probiotics are among the most attractive food supplements for promoting animal’s growth and health condition with the aim of increasing production and lowering losses. Four hundred and eighty fish weighing 31.8 ± 2.6 g were randomly divided into four groups each in triplicates. Groups 1 to 3 were fed with commercial carp feed containing 0...

The effects of different concentrations of Rosmarinus officinalis essential oil (EO) (0, 0.0015, 0.045, and 0.135 %) and nisin (N) (0, 0.25, and 0.5 µg/mL) as well as temperature (4 and 8 ºC) and storage time (up to 15 days) on growth of Streptococcus iniae and Lactococcus garvieae in fillets of Oncorhynchus mykiss were evaluated in a factorial des...

Grouper Iriovirus (GIV) is one of the most devastating viral diseases of marine and cultured groupers worldwide. In the current study, 5 presumptive Malaysian GIV isolates were characterised through PCR amplification of the major capsid protein (MCP) gene and phylogenetic analysis of the sequences. The sequences from the five GIV isolates showed 10...

BACKGROUND: Goldfish (Carassius auratus), a carp fish of Cyprinidae family, is one of the most popular aquarium fishes in Iran and also in the world. Ornamental fish trade, especially Goldfish, is aged over a hundred years. If we believe that any aquatic species or country has its own specific and unknown fauna of pathogens, the risks of transmissi...

Infectious pancreatic necrosis virus (IPNV) is the etiological agent of a contagious viral disease causing remarkable mortalities in different fish species. Despite the availability of commercial vaccines against IPN, the disease still constitutes one of the main threats to the aquaculture industry worldwide. In this study, we developed a DNA vacci...

This study was undertaken to evaluate the role of polychaete Nereis diversicolor in bioremediation of waste water and its growth performance and fatty acid composition in an integrated culture system with great sturgeon, Huso huso. Three treatments consisting of N. diversicolor fed with H. huso waste (FNW), N. diversicolor fed with fish feed waste...

The study of probiotic application as an important rearing strategy was started more than 30 years ago and most of these studies were carried out to increase growth and survival of larvae. Effect of Bifidobacterium animalis PTTC-1631 and B. lactis PTTC-1736 as probiotic supplement has been studied on growth performance and hematological changes on...

The study of probiotic application as an important rearing strategy was started more than 30 years ago and most of these studies were carried out to increase growth and survival of larvae. Effect of Bifidobacterium animalis PTTC-1631 and B. lactis PTTC-1736 as probiotic supplement has been studied on growth performance and hematological changes on...

This study investigated the effect of dietary Zn levels on growth performance, feed utilization, and hematological parameters of juvenile Siberian sturgeon (Acipenser baerii, Brandt 1869). The five semipurified diets were formulated by adding 0, 5, 10, 20, and 40 mg Zn kg-1 diet in the form of zinc sulfate (ZnSO4) to provide the actual dietary valu...

In this study the genetic diversity of wild Caspian trout (Salmo trutta caspius) in the Sardabroud and Astara Rivers was evaluated using D-Loop region sequencing. For this purpose, 35 specimens of adult Caspian brown trout were collected from these rivers in the Mazandarn and Gilan Provinces in fall and winter 2011. Approximately 3-5 g of soft and...

This study was conducted with different levels (0, 0.25, 0.5,1,2 g) of Zingiber officinale powder per 100 g of common carp commercial diet. Feed was offered for 8 weeks by the post pelleting liquid spraying method. Results showed that there were significant differences in all growth parameters and body composition except in survival rate, Gain Weig...

This study aimed to examine the effects of Chlorella vulgaris (CV) on the hematological and immune parameters of Koi carp (Cyprinus carpio). An experiment was designed with five treatments, including diets with 0, 2, 5, 7 and 10% of C. vulgaris each with three replicates for 8 weeks. At the end of experiment, the hematological and, immune system pa...

In the present study, rainbow trout weighting 3±0.3 g were vaccinated with an oral DNA vaccine encoding VP2 gene of a prevalent isolate of IPNV in Iranian trout farms encapsulated in sodium alginate microspheres and Chitosan tripolyphosphate (CS/TPP) nanoparticles. The vaccinated fish were then challenged with a virulent isolate of IPNV at 30 days...

Infectious hematopoietic necrosis virus (IHNV) is the etiological agent of a contagious disease (IHN) mainly in salmonid fish. In the present study, we isolated and identified IHNV in trout fry from Iranian trout farms with unexplained high mortality in 2016. The affected fry showed cumulative mortality of 90% with the gross pathological signs incl...

This study evaluated the effect of whey protein concentrate (WPC) coating incorporated with 1.5% cinnamon essential oil (CEO) on the quality of beluga sturgeon (Huso huso) fillet during refrigerated storage (4 ± 1C). Microbial, chemical, and sensorial properties of the fillets were measured for 20 days. Whey protein edible coating containing 1.5% C...

Iridovirus is the causative agent of grouper iridovirus infection (GIV), which causes severe epizootics resulting in large-scale mortalities and huge economic losses in cultured and marine fishes worldwide. The current study evaluated the gross and histopathological lesions, and molecular detection of GIV in naturally infected grouper farms in Mala...

Despite several reports on the presence of the potentially zoonotic nematodes among edible fishes in the Persian Gulf, there is still no study on the specific identification of these parasites or their genetic characterisation. In the present study, a total of 600 fish belonging to five popular species of fish in the region, including Otolithes rub...

An investigation was conducted in order to determine the etiological factors of Fry Mortality Syndrome (FMS) that causes a serious economical loss in rainbow trout's farms in Iran and around the world. The increased number of farms and the improvement of culture techniques and facilities in Iran had boosted the annual production of trout from 280 t...

The effects of orally administered purple cone flower Echinacea purpura and garlic (Allium satvium) on the non-specific immunity and growth condition of beluga weighing 1.15 ± 0.02 kg were evaluated for a period of 60 days. The tree groups of fish were fed two diets containing 0.5% purple coneflower and 1% garlic and a control diet containing no su...

Viral hemorrhagic septicemia virus (VHSV) is a rhabdovirus that causes one of the most important fish diseases in rainbow trout (Oncorhynchus mykiss) production industry. During the present study from October 2014 to July 2015, the virus causing viral hemorrhagic septicemia (VHS) was isolated and identified in rainbow trout farms from five of sixte...

To examine the effect of dietary silver nanoparticles (Ag-NPs) on different organs of D. rerio as well as evaluating of its retention and toxicity under laboratory conditions. Four experimental diets were designed with a concentration of 1, 4, 6 and 8 mg.kg-1 (ppm) nanoparticles in the diet (named C1, C4, C6 and C8, respectively) and control group...

This study was carried out to survey the effect of immune motivator Macrogard and Spirulina platensis on some hematological and immunophysiological parameters of young cultured stellate sturgeon Acipenser stellatus. This investigation was designed using a completely random plan containing 0%, 0.1% and 0.5% Macrogard and Spirulina in 5 treatments wi...

The effects of different doses of date palm (Phoenix dactylifera) seed (DPS) extract on heavy metals (Pb, Cd, As, Hg) concentrations of common carp (Cyprinus carpio) were evaluated. The fish (31.6±3.7 g) were assigned to five treatments of three replications each. Five treatments were held using five different DPS extracts at doses of 0% (control),...

Background: Strepotococcosis caused by Streptococcus iniae is one of the important emerging bacterial diseases in aquaculture sector worldwide. Objectives: In this study, the genetic diversity of S. iniae strains was assessed in some rainbow trout farms in Iran. Methods: Gram positive and catalase negative bacterial isolates were first obtained fro...

BACKGROUND: Lactococcus lactis is one of the lactic acid bacteria that can be considered as a probiotic in Persian sturgeon. OBJECTIVES: The aim of the present study was to increase the resistance to Aeromonas hydrophila after feeding the L. lactis JF831150. METHODS: In the present study, first 60 fish were fed different doses of L.lactis JF831150...

Yersiniosis is an economically important bacterial disease affecting the aquaculture industry. Some immune response parameters and efficacy of intraperitoneal (IP) injection of Yersinia ruckeri bacterin (biotype I) formulated with Montanide ISA 763 AVG adjuvant were assessed in 100 g rainbow trout during 2-12 weeks post-vaccination. Fish were immun...

Yersiniosis is an economically important bacterial disease affecting the aquaculture industry. Some immune response parameters and efficacy of intraperitoneal (IP) injection of Yersinia ruckeri bacterin (biotype I) formulated with Montanide ISA 763 AVG adjuvant were assessed in 100 g rainbow trout during 2-12 weeks post-vaccination. Fish were immun...

Pick handle barracuda (Sphyraena jello) is one of the most important commercial species in the Persian Gulf. In order to investigate prevalence and intensity of nematoda, 150 S. jello from three sites (Khuzestan, Bushehr and Hormozgan provinces) of the Iranian coast of the Persian Gulf were monthly investigated in the period 2012-2013. The nematode...

Introduction: Stressful condition in fish is inevitable and cause increasing in cortisol level of plasma, suppress of immune system, catching diseases and can death. Using some anesthetic composition can reduce metabolism and stress, thus increase fish survival. But chemical and herbal material have some restrictions. The derm of palm bloom known a...

Genetic diversity of six populations of bartail flathead (Platycephalus indicus Linnaeus, 1758) was investigated using amplified fragment length polymorphism (AFLP). A total of 118 reproducible bands amplified with ten AFLP primer combinations were obtained from 42 fishes that were collected from six different locations in the northern part of the...

Silver nanoparticles (AgNPs) are known to have bactericidal and fungicidal effects. Since, there is few information available on the interaction of colloidal nanosilver with fish pathogens. Hence, the current study investigated the effects of colloidal AgNPs on the in vitro growth of the fish pathogen Saprolegnia sp.. Before the experiments, variou...

One application of medicinal plants is their use as antimicrobial agents. The aim of this study was to investigate the effect of essential oils on four species of fungi in rainbow trout (Oncorhynchus mykiss) eggs, including Saprolegnia parasitica, Aspergillus niger, Aspergillus fumigates, Aspergillus flavus. Essences minimum inhibitory concentratio...

To determine the incidence of onychomycosis based on age and sex, morphological pattern of the disease, predisposing factors and identification of fungus by direct microscopy and culture methods. A prospective study was conducted on 140 patients with nail disorders. A detailed history and thorough examination was done in all patients. The samples w...

In this study the genetic diversity of wild Caspian trout (S. trutta caspius) population migrating to the Astara and Chalus Rivers was evaluated using D-Loop sequencing. For this purpose, 47 specimens of adult Caspian brown trout were collected from these rivers in the Gilan and Mazandarn provinces in fall and winter 2011 approximately 3-5 g of sof...