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Introduction
Meenu Mridula currently works at the Department of Botany, University of Kerala. Meenu does research in plant tissue culture with reference to identification of bio synthetic pathway of quinazoline alkaloids.
Publications
Publications (3)
In this paper, we compared the efficacy of observation based modeling approach using a genetic algorithm with the regular statistical analysis as an alternative methodology in plant research. Preliminary experimental data on in vitro rooting was taken for this study with an aim to understand the effect of charcoal and naphthalene acetic acid (NAA)...
The minimal data set used for the analysis.
Legends NAA, CH, BC, R, RL, NR and NRL represent the concentration of NAA, concentration of charcoal, basal callus diameter, percentage of shoots rooted, length of longest roots, number of roots and number of lateral roots, respectively.
(CSV)
A protocol for in vitro production and acclimatization of wrightia tinctoria (rox.) R. Br., is reported. Under the regime used in the study, the most critical factor for rooting was activated charcoal (0.05%) along with 6 μM Naphthalene aceticacid. The plantlets hardened with longer photoperiods had better survival percentages when compared with th...
Questions
Questions (4)
I found this floating in a fresh water tub containing rotting vegetation and coco peat.
I am interested in augmenting a phyto-compound in vitro; and to that end i tried some elicitors to plant cell suspension cultures. In a system where methyl jasmonate was used i was able to collect the desired compound as evidenced by running a co-TLC against its standard that was purchased.
The problem surfaced when i viewed the TLC plates in far UV; where i got around 9 different fluorescent bands corresponding to some other compounds. So even though these are not of my primary interest, its still obligatory to know what those other things are. Scraping each of them and doing a NMR is beyond my scope given the time and the initial quantity of sample. Is there any other way to identify any of these bands? Will doing an LCMS analysis of the sample and comparing the molecular mass with previous reports be a viable option?
I have attached the picture of the TLC plates as seen in UV light. For our consideration we are taking only the first lane.. and then the last lane which is the standard that i purchased.
Any suggestion is highly appreciated.
Hey,
I had done the NMR analysis of a crystal that i suppose is Typtanthrin ( C15H8N2O2). The crystal was dissolved in CDCl3 for the analysis. I am given a plot as the results and i can not make any progress with its interpretation. Can anyone help me make progress with its interpretation. I dont have any expertise with NMR data and any help is highly appreciated.
I have attached the plots for perusal.
Thank you
When plantlets are obtained by direct organogenesis from nodal explants, is there a need to confirm its genetic fidelity to mother plant by means of ISSR or PCR?
