Md. Shahinozzaman

Md. Shahinozzaman
University of Maryland, College Park | UMD, UMCP, University of Maryland College Park · Department of Nutrition and Food Science

Ph.D.

About

48
Publications
35,127
Reads
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391
Citations
Introduction
Currently involved in identifying potential botanicals/natural therapeutics to modulate obesity, insulin resistance, and type 2 diabetes and thereby developing functional foods for human health benefits.
Additional affiliations
April 2019 - present
University of the Ryukyus
Position
  • PostDoc Position
Description
  • Research Grant: PAK Research Center, University of the Ryukyus Research Focus: Bioactive Compounds, Organic Synthesis, Drug Discovery, PAK1
April 2016 - March 2019
Kagoshima University
Position
  • PhD Student
Description
  • Allied University: University of the Ryukyus Research Focus: Biological resources, Medicinal Plants, Bioactive Compounds, Pharmacology
October 2015 - March 2016
University of the Ryukyus
Position
  • Visiting Research Student
Description
  • Research Grant: PAK Research Center, University of the Ryukyus Research Focus: Natural Products Chemistry, Cell Culture
Education
April 2016 - March 2019
University of the Ryukyus
Field of study
  • Agricultural Science
May 2009 - February 2011
University of Rajshahi
Field of study
  • Botany (Plant Biotechnology)
April 2004 - April 2009
University of Rajshahi
Field of study
  • Botany

Publications

Publications (48)
Article
Full-text available
Acetylcholinesterase is a promising therapeutic candidate for the treatment of neurodegenerative disorders, acetylcholine dysfunction, and other cognitive problems. In the current study, a 3D-QSAR approach was applied to a series of benzimidazole derivatives to reveal the key influencing factors contributing to their acetylcholinesterase inhibition...
Article
Full-text available
The genus Bulbophyllum is of scientific interest due to the phytochemical components and diverse biological activities found across species of the genus. Most Bulbophyllum species are epiphytic and located in habitats that range from subtropical dry forests to wet montane cloud forests. In many cultures, the genus Bulbophyllum has a religious, prot...
Article
Full-text available
Tubulin plays an indispensable role in regulating various important cellular processes. Recently, it is known as a hopeful therapeutic target for the rapid division of cancer cells. Novel series of 2-oxoquinoline arylaminothiazole derivatives have been recently identified as promising tubulin inhibitors with potent cytotoxicity activity against HeL...
Article
Full-text available
P21-activated kinases (PAKs) are serine/threonine protein kinase which have six different isoforms (PAK1–6). Of those, PAK1 is overexpressed in many cancers and considered to be a major chemotherapeutic target. Most of the developed PAK1 inhibitor drugs work as pan-PAK inhibitors and show undesirable toxicity due to having untargeted kinase inhibit...
Article
Full-text available
Cruciferous vegetables have been widely studied for cancer prevention and cardiovascular health. Broccoli is the cruciferous vegetable whose phytochemistry and physiological effects have been most extensively studied. Kale ( Brassica oleracea var. acephala) appears on lists of ‘healthiest, nutrient dense foods’ but, there is paucity of data on kale...
Article
Full-text available
Several plants have traditionally been used since antiquity to treat various gastroenteritis and respiratory symptoms similar to COVID-19 outcomes. The common symptoms of COVID-19 include fever or chills, cold, cough, flu, headache, diarrhoea, tiredness/fatigue, sore throat, loss of taste or smell, asthma, shortness of breath, or difficulty breathi...
Article
Full-text available
Lambertianic acid (LA) is a diterpene bioactive compound mainly purified from different species of Pinus. It is an optical isomer of another natural compound daniellic acid and was firstly purified from Pinus lambertiana. LA can be synthesized in laboratory from podocarpic acid. It has been reported to have potential health benefits in attenuating...
Article
Chemotherapy is the main treatment used for cancer patients failing surgery. Doxorubicin (DOX) is a well-known chemotherapeutic agent capable of suppressing proliferation in cancer cells and triggering apoptosis via inhibiting topoisomerase II activity and producing DNA breaks. This activity of DOX restrains mitosis and cell cycle progression. Howe...
Article
Background Lung cancer is considered to be the first place among the cancer-related deaths worldwide and demands novel strategies in the treatment of this life-threatening disorder. The aim of this review is to explore regulation of epithelial-to-mesenchymal transition (EMT) by long non-coding RNAs (lncRNAs) in lung cancer. Introduction LncRNAs ca...
Article
Urtica dioica (UT) vegetable attenuates diet induced weight gain and insulin resistance. We hypothesized that UT imparts metabolic health by impacting the gut microbiota composition. We examined effects of UT on the cecal bacterial taxonomic signature of C57BL/6J mice fed isocaloric diets: a low-fat diet (LFD) with 10% fat, a high fat diet (HFD) wi...
Article
Full-text available
Kale (Brassica oleracea var. acephala) is a vegetable common in most cultures but is less studied as a functional food compared to other cruciferous vegetables, such as broccoli. We investigated the effect of supplementing a high-fat diet (HFD) with kale (HFKV) in C57BL/6J mice. We particularly explored its role in metabolic parameters, gut bacteri...
Article
Full-text available
Background: Ischemia/reperfusion (I/R) injury is a serious pathologic event that occurs due to restriction in blood supply to an organ, followed by hypoxia. This condition leads to enhanced levels of pro-inflammatory cytokines such as IL-6 and TNF-, and stimulation of oxidative stress via enhancing reactive oxygen species (ROS) levels. Upon reperf...
Article
Artepillin C (ARC), a prenylated derivative of p-coumaric acid, is one of the major phenolic compounds found in Brazilian green propolis (BGP) and its botanical source Baccharis dracunculifolia. Numerous studies on ARC show that its beneficial health effects correlate with the health effects of both BGP and B. dracunculifolia. Its wide range of pha...
Article
Gastrointestinal (GI) cancers with a high incidence rate and adverse complications are related to severe morbidity and mortality around the world. MicroRNAs (miRs) are potential regulators of cellular events, and their aberrant expression occurs in gastrointestinal (GI) cancers. Increasing evidence demonstrates that plant derived-natural compounds...
Article
Propolis, a resinous substance, is collected from plants and processed by honeybees to seal holes and cracks in beehives, protecting them from microbial infection. Based on the plant source and geographical location, propolis is categorized into seven groups. Of these, Pacific propolis, found in the Pacific islands, originates from Macaranga spp. a...
Article
Transcription factors are potential targets in disease therapy, particularly in cancer. This is due to the fact that transcription factors regulate a variety of cellular events, and their modulation has opened a new window in cancer therapy. Sex-determining region Y (SRY)-related high-mobility group (HMG) box (SOX) proteins are potential transcript...
Article
Full-text available
The shoot of Urtica dioica is used in several cultures as a vegetable or herb. However, not much has been studied about the potential of this plant when consumed as a whole food/vegetable rather than an extract for dietary supplements. In a 12-week dietary intervention study, we tested the effect of U. dioica vegetable on high fat diet induced obes...
Article
Improving the nutritional quality of germinated brown rice (GBR) is important for the population consuming rice as part of the daily diet to offer health benefits. In this study, a set of popularly grown 79 rice varieties in Southern Vietnam was evaluated to identify the potential materials for producing gamma-aminobutyric acid (GABA)-enriched GBR....
Conference Paper
Malignant neoplasm is associated with inappropriate activation of the apoptosis. Apoptosis plays crucial roles in cell cycle regulation and tissue development; however, this process is believed to be aberrant, when the inhibitor of apoptosis proteins (IAPs), particularly, survivin is directly interfering with caspases during the apoptosis mechanism...
Article
Full-text available
Type 2 diabetes (T2D) is generally characterized by elevated blood glucose level, insulin resistance, and relative lack of insulin, however, insulin resistance is the predominant risk factor. Hence, the use of insulin sensitizer drugs to increase insulin sensitivity have gained immense interest as an attractive treatment option for T2D and their ma...
Article
Full-text available
The oncogenic kinase PAK1 (p21-activated kinase 1) is involved in developing many diseases including cancers, neurofibromatosis, Alzheimer's disease, diabetes (type 2), and hypertension etc. Thus, it is thought to be a prominent therapeutic target, and its selective inhibitors have a huge market potential. Recently, herbal PAK1 inhibitors have gain...
Article
Full-text available
Medicinal plants belonging to the genus Ardisia are traditionally used to cure various human diseases including inflammation and cancer. This study aimed to purify and characterize cytotoxic and anti-inflammatory compounds from Ardisia sieboldii leaves. Bioassay-guided chromatographic analyses yielded three compounds, 2-methyl-5-(8 Z -heptadecenyl)...
Poster
Full-text available
Vascular endothelial growth factor-A (VEGF-A) is one of the most important growth factors being essential for tumor angiogenesis. It is secreted by the tumor cells and tumor-associated stromal cells, which subsequently enhances vasculogenesis and neoangiogenesis to supply oxygen and nutrients to actively proliferating tumor cells for their growth a...
Article
Full-text available
Okinawa propolis (OP) and its major ingredients were reported to have anti-cancer effects and lifespan-extending effects on Caenorhabditis elegans through inactivation of the oncogenic kinase, p21-activated kinase 1 (PAK1). Herein, five prenylated flavonoids from OP, nymphaeol-A (NA), nymphaeol-B (NB), nymphaeol-C (NC), isonymphaeol-B (INB), and 3′...
Article
Full-text available
Sea cucumbers have long been utilized in foods and Asiatic folk medicines for their nutritive and health benefits. Herein, three sea cucumber species were investigated and Holothuria atra showed the highest cytotoxicity among these. Next, a desulfated saponin, desulfated echinoside B (DEB), was purified from H. atra through bioassay-guided fraction...
Article
Full-text available
Background: The leaves of Alpinia zerumbet var. excelsa (alpinia) are used to prepare traditional food items and folk medicines. Objective: This study was designed with a view to explore the anti-inflammatory and anti-melanogenic potentials of major components of alpinia leaves. Materials and Methods: Anti-inflammatory effects of leaf-derived compo...
Article
Full-text available
Muscari armeniacum is one of the important ornamental cut flowers in floriculture industry, which is native to Southern Europe, North Africa, Western Asia and Asia Minor. In this study, bulb explants (basal plate of bulb having meristems), bulb scales, and leaf segments from in vitro derived bulblets were cultured in Murashige and Skoog (MS) medium...
Poster
Alpinia zerumbet is a perennial medicinal plant, which leaves are used to prepare traditional food items and folk medicines. Five major compounds- (E)-5-methoxy-8-(4-methoxy-2-oxo-2H-pyran-6-yl)-7-phenyl-1-styryl-2-oxabicyclo[4.2.0]oct-4-en-3-one (AS-2), 5,6-dehydrokawain (DK), dihydro-5,6-dehydrokawain (DDK), kaempferol 3-O-β-D-glucuronide (KOG),...
Article
Full-text available
Artepillin C (ARC) and caffeic acid (CA) are among the major anti-cancer ingredients of propolis, and block the oncogenic/melanogenic/ageing kinase PAK1. However, mainly due to their COOH moiety, cell-permeability of these herbal compounds is rather limited. Thus, in this study, in an attempt to increase their cell-permeability without any signific...
Article
Full-text available
In the present study, preliminary in vitro shoots propagation of Curcuma caesia Roxb. was investigated. Rhizome buds were used as explants and were cultured on Murashige and Skoog (MS) medium containing 6-Benzyl adenine (BA) alone or in combination with α-Naphthalene acetic acid (NAA). The results showed that the optimum shoot proliferation was obt...
Data
Using cotyledons of 2-week old in vitro germinated seedlings, a rapid and efficient regeneration protocol was established for A. mangium on Murashige and Skoog (MS) basal medium. Cotyledons showed better direct shoot organogenesis producing maximum 10.88 ± 0.35 shoots per explant when cultured on MS medium supplemented with 2.0 μM BA and 1.0 μM NAA...
Article
Full-text available
Using cotyledons of 2-week old in vitro germinated seedlings, a rapid and efficient regeneration protocol was established for A. mangium on Murashige and Skoog (MS) basal medium. Cotyledons showed better direct shoot organogenesis producing maximum 10.88 ± 0.35 shoots per explant when cultured on MS medium supplemented with 2.0 µM BA and 1.0 µM NAA...
Article
Full-text available
In the present study, preliminary in vitro shoots propagation of Curcuma caesia Roxb. was investigated. Rhizome buds were used as explants and were cultured on Murashige and Skoog (MS) medium containing 6-Benzyl adenine (BA) alone or in combination with α-Naphthalene acetic acid (NAA). The results showed that the optimum shoot proliferation was obt...
Article
Full-text available
An efficient protocol for in vitro clonal propagation of A. mangium was developed using seedling derived explants. Out of three different explants tested for shoot proliferation, cotyledonary node showed best performance than leaf node and shoot tip explants. MS (Murashige and Skoog�s) medium was found best for shoot proliferation and cotyledonary...
Data
An efficient protocol for in vitro clonal propagation of A. mangium was developed using seedling derived explants. Out of three different explants tested for shoot proliferation, cotyledonary node showed best performance than leaf node and shoot tip explants. MS (Murashige and Skoog's) medium was found best for shoot proliferation and cotyledonary...
Article
Full-text available
An investigation was undertaken to develop a reliable strategy for large scale multiplication of a hybrid cultivar of muskmelon through in vitro clonal propagation. Best in vitro seed germination was observed in MS medium containing 0.5 mg/l GA3. Two explants excised from axenic seedlings were tested for shoot proliferation and leaf node showed bet...
Article
Full-text available
This paper describes efficient in vitro propagation protocol of Curcuma amada Roxb., an important aromatic zingiberaceous plant in the countries of Indian subcontinent, using rhizome explants. Rhizome tip explants showed better response than rhizome bud explants in terms of shoot proliferation and hence excised rhizome tip explants were inoculated...

Questions

Questions (14)
Question
With the active fractions from Si-gel CC, I did high resolution LC-MS/MS experiments and got the spectrum. However, I haven't find any reference of purifying the compounds from the same sample. Could anyone suggest me how to search the database with MS spectrum to get the chemical structure?
Or which database can I sear for my purposes?
Question
I am interested in Molecular dynamics calculation. After finishing the docking, I would like to carry out dynamics calculation to understand the stability of the ligand-protein pose. If anyone can help me I would be grateful.
Please suggest me some good tutorial, books, articles or manuals etc.
Question
Can anyone tell me how to search AIST database with proton/carbon NMR chemical shifts? Before I tried, but in the long run failed to get the suggested chemical structure. Your cooperation would highly be acknowledged.
Question
I have collected one active fraction from Si-gel CC using Hexane:EtOAc gradient. The active fraction eluted with 3:2 ratio. Now its time to purify the active compounds from this fraction. TLC analysis shows the presence of many spots, that's why now which method I need to follow to purify the compounds?
Expert suggestion would highly be acknowledged.
Question
I would like to elucidate chemical structure of an unknown sample using proton-NMR, Carbon-NMR, 2D-NMR and Mass spectrum Data. Can anybody suggest me how to do this and what type of references I need to study to learn this?
Thanks in advance.
Question
I am using Nicotinic acid for synthesizing some ester derivatives. I am using EDC/DMAP/DMF. Whenever I tried to purify the ester from reaction mixer with EtOAc or EtOAc-Hexane solvents (liquid-liquid extraction), I was unable to purify the target? Can anyone suggests me how to purify the esters from the reaction mixture?
Question
I would like to prepare Tolmetin from Tolmetin sodium salt dihydrate (TSSD). TSSD is an commercial product, however I would like to work with Tolmetin only.
Question
I would like to know the details about the reaction attached.
It would be highly acknowledged in any one can explain it to me. 
Thanks in advance.
Question
Now, I am planning to synthesize some Alkyne derivatives using the -COOH moiety of some target natural compounds. I know about the Fishcer Esterification using DMF as a slovent and DMAP and EDC.HCl as a catalyst while using Propargyl Alcohol as a reactant with Carboxyllic acid.
Another route, I know that using Propargyl Halides as reactant with Carboxyllic acid, and in this case THF needs to use as solvent and Caesium carbonate as catalyst.
I would like to know the expert opinions to produce Alkyne derivatives from the Carboxyllic acids. Your opinion would highly be acknowledged.
Thanks in advance.
Question
I would like to stain the cells with DAPI. I am using 24 well plate for cell culture. Is it possible to stain and observe under fluorescence microscope using the well plate directly? Could anyone help me to provide the detail protocol for it?

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Projects

Projects (3)
Project
Studying terrestrial and marine resources. Exploring their pharmacological activities as anti-inflammatory, anti-melanogenesis and anti-cancer therapeutics. Purification of bioactive components. Studying computational chemistry and in silico studies.