Md. Mer Mosharraf HossainJessore University of Science and Technology | JSTU · Department of Fisheries and Marine Bioscience
Md. Mer Mosharraf Hossain
BSc, MS, PhD
About
95
Publications
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Introduction
Current Research Interest:
Primer Design, DNA or RNA isolations, PCR diagnosis of DNA products, Gel /PAGE electrophoresis of PCR products, DNA quantifications, Genomic sequence of PCR products. Gateway Gene cloning technology. TiLV, EMS, AHPNS diseases, (SPSS, NTSYS, NTSYS-UPGMA, Power Marker software, POPGENE, BLAST, MEGA-X, Chromas, SegMAN, DNAMAN, and Genemarker, etc.
Publications
Publications (95)
The metal contamination levels in soil, water, and fish samples from the Sundarbans in Bangladesh were thoroughly examined. Key pollutants were identified using Principal Component Analysis (PCA), and pollution and health risks were evaluated using indices like TRI, Igeo, and Pearson correlation coefficients. Metal concentrations (Mn, Cu, Zn, Pb, C...
This study investigated the impact of dietary guava leaf extract (GLE) on immunity, resistance to Pseudomonas aeruginosa infection, and growth in Oreochromis niloticus (Nile tilapia). Four groups of fish (28.6 g.fish−1) were fed diets supplemented with ethanolic GLE at 0 (control), 3, 5, or 7 g.fish−1 diet (GLE0diet, GLE3diet, GLE5diet, GLE7diet) a...
The study aimed to address the recurring outbreaks of microbial diseases in shrimp aquaculture in Bangladesh the study focused on the utilization of bacteriophages and non-pathogenic Vibrio. The bacteriophages were isolated from sewage water sample collected from shrimp farm, hatchery, and the JUST campus. The bacte-riophages were tested for their...
A R T I C L E I N F O Keywords: 16S rRNA gene Pathogenic bacteria Non-pathogenic bacteria The Sundarbans mangrove Finfish and shellfishes A B S T R A C T The Sundarbans, located in Bangladesh, is the world's largest mangrove forest with a rich and diverse microbial community. The main objective of this study was to use 16S rRNA sequencing to identi...
Concerns have been conveyed regarding the availability and hazards of microplastics (MPs) in aquatic biota due to their widespread presence in aquatic habitats. Zebrafish (Danio rerio) are widely used as a model organism to study the adverse impacts of MPs due to their several compelling advantages, such as their small size, ease of breeding, inexp...
This study investigated the impact of dietary guava leaf extract (GLE) on immunity, resistance to Pseudomonas aeruginosa infection, and growth in Oreochromis niloticus (Nile tilapia). Four groups of fish (28.6 g.fish−1) were fed diets supplemented with ethanolic GLE at 0 (control), 3, 5, or 7 g.fish−1 diet (GLE0diet, GLE3diet, GLE5diet, GLE7diet) a...
The study aimed to address the recurring outbreaks of microbial diseases in black tiger shrimp ( Penaeus monodon) aquaculture in Bangladesh by utilizing bacteriophages and non-pathogenic Vibrio . Bacteriophages were isolated from sewage water collected from a shrimp farm, hatchery, and the JUST campus. The infectivity of the bacteriophages against...
Vibrio parahaemolyticus, the causative agent of Acute hepatopancreatic necrosis disease (AHPND), was discovered in 2013 as a unique isolate that produces toxins and kills penaeid shrimps in devasting nature in Bangladesh and causes severe economic losses. This research aimed to understand the expressions of immune genes in different stages of the h...
The microbial environment of the Sundarbans mangrove forest contains sediments and water usually more diverse than the other aquatic environment, and pathogenic for finfish and shellfishes will be revealed by phylogenetic tree analysis of the positive controls PCR product. Approximately, 21 samples of the soil, 21 samples of water, 21 samples of fi...
Acute hepatopancreatic necrosis disease (AHPND) is an emerging shrimp (Penaeus monodon) disease caused by Vibrio parahaemolyticus (VP) since 2013 in Bangladesh. The aim of this work was to evaluate a PCR and RT-PCR techniques as rapid methods for detecting V. parahaemolyticus AHPND-positive P. monodon using genetic markers. Healthy and diseased shr...
The present study was conducted to evaluate the effects of dietary nano-nutrients on the growth, physiological and amino acid responses in tilapia fish. Vitamins were incorporated with chemically synthesized nanoparticles (Fe, Zn, Cu and Se) to form a nano-nutrient complex (NNC). Powder X-ray diffraction (PXRD) and scanning electron microscopy (SEM...
This study aimed to investigate the effect of black cumin seed oil (BCSO) on the growth metrics, innate immunity and resistance against Pseudomonas fluorescens infection in Nile tilapia Oreochromis niloticus. This was done by conducting a feeding trial experiment on the fish. A total of four diets (protein, 35.99 ± 0.16%; lipid, 9.86 ± 0.06%) varyi...
This study aimed to investigate the effect of black cumin seed oil (BCSO) on the growth metrics, innate immunity and resistance against Pseudomonas fluorescens infection in Nile tilapia Oreochromis niloticus. This was done by conducting a feeding trial experiment on the fish. A total of four diets (protein, 35.99 ± 0.16%; lipid, 9.86 ± 0.06%) varyi...
Background and objectives: Tilapia lake virus (TiLV) has been marked as an emerging infectious agent that causes mass die-offs in farmed mono-sex Nile tilapia (Tilapia niloticus) in Bangladesh, indicates rapid diagnostic assay. This study was aimed to develop molecular detection method to confirm the TiLV in Tilapia niloticus and construct a geneti...
To control of Edwardsiella tarda in the freshwater cultured fish species of Bangladesh that recorded massive threat to indigenous species, and caused the severe infestations. This study was aimed at the determination of the E. tarda bacteria in freshwater cultured fish species and construct a genetic baseline to control this pathogen. This study wa...
The invasive mosquito fish Gambusia affinis, affected hatchery populations and abundant in different wetlands of Jashore regions which carry ectoparasites. This study evaluated genetic diversity between hatchery and wild populations of G. affinis using polymorphic SSR DNA markers, which have not yet been clarified. A total of 50 polymorphic SSR and...
Background and objectives: To improve the sustainable management and control of North American, western mosquitofish (Gambusia affinis) in the freshwater of Bangladesh, the most invasive to indigenous species, caused the severe infestation to all carps, genetic diversity of the species were studied using DNA sequences of nucleotides first time in B...
The aim of this study was to evaluate the biology of a small fish, darkina, Esomus danricus collected from natural pond located in the Fisheries Field Laboratory complex, BAU, Mymensingh. The experiment was undertaken in a pond using thirty six small hapas (0.25 m 3) and one big hapa (1 m 3). All the hapas were placed in a big pond (420 m 2). The s...
A survey was carried out to investigate culture strategies, disease patterns and mitigations in mono-sex Nile tilapia in Jessore sadar region. Data obtained by questionnaire interview, individual interview, telephonic interview, PRA method, mono-sex Nile tilapia farm survey from four villages. It was observed that 30.769% farmers and farm owner had...
Lernaea cyprinacea (Anchor worm), a parasitic cyclopoid copepod is found to parasitize in the
broodstock, nursery, and culture system in the freshwater aquaculture ponds and hatchery of Jessore
regions. Lernea sp. infestations found in major carps (Indian & Chinese), minor barbs, catfish and
perches. Total eight hundred (800) fish of different host...
This study was carried out in different water bodies to assess production potentials on carp-mola polyculture commonly found from July-December 2012. Pond and pond like inundated rice plots were employed for the experiment. It was comprised of three treatments with having 12 replicate in two districts. The examined species: rohu (Labeo rohita), cat...
An experiment was conducted to study the effect of different feeds on growth and production of freshwater Mud Eel (Monopterus cuchia) in nine aquariums over a period of 90 days during June to August 2016. Three different feeds namely Feed A (live feed), Feed B (supplementary feed) and Feed C (probiotics) were applied to treatments I, II and III res...
An investigation was conducted for simultaneous production of rice and fish in framers' plot from July-December 2012 in Northwest region of Bangladesh. It consisted of three treatments and each having five replicates. The experimental species were rui (Labeo rohita), catla (Catla catla), mrigal (Cirrhinus cirrhosus), silver carp (Hypophthalmichthys...
The present study evaluated the efficacy of dietary probiotic Biozyme on the immunological parameter and disease resistance against Pseudomonas sp. infection in Vietnam Koi (Anabas testudineus). Fish with an average body wt. 25±5 g were fed twice a day at 5% of their body wt.) experimental diet prepared with the supplementation of 0 g, 0.5 g, 1.0 g...
Lernaea cyprinacea (Anchor worm), a parasitic cyclopoid copepod is found to parasitize in the broodstock, nursery, and culture system in the freshwater aquaculture ponds and hatchery of Jessore regions. Lernea sp. infestations found in major carps (Indian & Chinese), minor barbs, catfish and perches. Total eight hundred (800) fish of different host...
A study was carried out to investigate the drugs and medicine used in aquaculture activities in greater Comilla region in Bangladesh for fish health management and increasing aquaculture production. Questionnaire interview, personal contact, market survey and participatory rural appraisal like focus group discussion were conducted to collect the da...
Green water indoor tank culture (GWT) of Tilapia with carp is an appropriate method for commercially producing of Tilapia in substitutional of different water bodies likes ponds, lakes, cages and reservoirs etc. that have environmental constraints such as land use conflicts, source of water, water quality and sub optimal temperatures, where a green...
Indian major carp, rohu, (Labeo rohita) (30±6g), were fed with two types of diets; an experimental diet, containing root extract (0.5%) of Achyranthes aspera as an ingredient and control diet without the root extract. Experimental rohu were injected intraperitoneally with Pseudomonas fluorescens (3.2×10-6 CFU ml-1) and bloods were sampled 14 and 28...
Plants are being explored as a major source of medicinal compounds. The present study was intended to explore the efficacy of dietary doses of Psidium guajava leaf extracts mixed with Maize bran (control) on growth performance, immunological parameters and disease resistance against Pseudomonas fluorescens infection in mono-sex Nile tilapia, Oreoch...
To study the effect of different stocking densities on the growth, survival and production of the freshwater prawn the post larvae with mean initial weight of 0.22±0.04g were stocked at 40, 50, and 80PL/decimal and cultured for a period of 150 days under gher conditions. The culture ponds were manured with cow dung and inorganic fertilizers and a s...
Aims: The present study evaluated the efficacy of Achyranthes aspera extract on immunological parameters and disease resistance against Pseudomonas fluorescens infection in Indian major carp, Rohu, Labeo rohita. Study Design: One hundred and eighty (180) fish with average weight (28.1±1.2 g) were acclimatized and stocked in (6) indoor aquariums (10...
An effective vaccine against Edwardsiella tarda has not been reported in substitution for high concentration of formalin for the prevention of edwardsiellosis disease. In this study, the efficacy of inactivated E. tarda was evaluated and compared by intraperitoneal (IP) injection-immunization or challenge against Japanese eel (Anguilla japonica). F...
The use of probiotics in aquaculture is now gaining a wide acceptance. This study was conducted to evaluate the commercially available probiotic Bioprob (PIC-BIO company, Tokyo, Japan) on the efficacy of its use on mean immune parameters and Pseudomonas fluorescens disease challenge in Thai pangas (Pangasius hypophthalmus) under laboratory conditio...
The effect of Nigella sativa (black cumin) seed extracts supplemented diets at 2%, 4% and 6% doses with common diet as control (0%) fed to common carp, Cyprinus carpio against Pseudomonas fluorescens was investigated critically on weeks 1, 2 and 4 as immunomodulator. Immunological factors including bactericidal activity and phagocytic action were e...
The effect of Nigella sativa (black cumin) seed extracts supplemented diets at 2%, 4% and 6% doses with common diet as control (0%) fed to common carp, Cyprinus carpio against Pseudomonas fluorescens was investigated critically on weeks 1, 2 and 4 as immunomodulator. Immunological factors including bactericidal activity and phagocytic action were e...
Streptococcus iniae is an important pathogen that can cause a broad range of disease in aquatic animals. To avoid the use of antibiotics and drugs, it is critical to identify protective antigens for developing highly effective vaccines against this pathogen. Vaccination is the most effective means of preventing infectious diseases; however, few vac...
Pangus (Pangasius pangasius) has been increasing for its high potential and play vital role in aquaculture in Bangladesh. The effects of dietary A. aspera supplementation on innate immunity, growth and disease against P. fluorescens infection were evaluated in Pangus (P. pangasius). Fish were feeding for 28 days with control diet and three experime...
This study showed the uses of garlic (Allium sativum) supplemented feed on Clarias batrachus for the judgment of garlic potential against Acanthocephala. Garlic allicin content (an active component of garlic) worked for C. batrachus to inhibit infection by Acanthocephala. Four garlic supplemented diets (50, 100 150 and 200 ml/kg) of different conce...
Labeo bata is one of the important minor carps in Bangladesh with great demand as good table fish. The study was conducted to observe the breeding performance at different management practices in Mafatema, Rupali, Modhumoti and Anan fish hatchery and disinfection treatments of water, eggs and fry in Jessore, Bangladesh during 10 March 2014 to 15 Ma...
Common carp (Cyprinus carpio) is one of the commercially important and commonly cultured fish. In the hatchery intensive incubation leads to microbial overgrowth in C. carpio eggs that hamper egg development, hatchability and larval survivability. The aim of this study is to find out causes of mass mortality in C. carpio eggs during peak- breeding...
The Recirculating Aquaculture Systems (RAS) with a biological filter is the most noticeable representative which treats internally the water polluted with dissolved organics and ammonia. It also decreases the amount of water use. This study showed lower microbial content and lesser toxic element in treated water than non-treated water. Among the tr...
An investigation was conducted to know the breeding biology of Vietnamese climbing perch (Anabas testudineus), locally known as Koi. Monthly samples were collected from Rupali Fish Hatchery throughout the period from January to July 2014. Five gross ovarian stages i.e. immature (mean ova diameter 0.17±0.05 mm), maturing (mean ova diameter 0.33±0.05...
Pangus (Pangasius pangasius) has been increasing for its high potential and play vital role in aquaculture in Bangladesh. The effects of dietary A. aspera supplementation on innate immunity, growth and disease against P. fluorescens infection were evaluated in Pangus (P. pangasius). Fish were feeding for 28 days with control diet and three experime...
The Recirculating Aquaculture Systems (RAS) with a biological filter is the most noticeable representative which treats internally the water polluted with dissolved organics and ammonia. It also decreases the amount of water use. This study showed lower microbial content and lesser toxic element in treated water than non-treated water. Among the tr...
This study deals with the isolation and characterization of parasitic infection in Channa punctata collected from different water bodies in Jessore, Bangladesh. Skins, gills, intestine, kidney, liver were observed for finding parasites. In total thirty parasites were identified under five species from fifty experimental fish. Different types of par...
Seven fresh bacterial isolates were isolated from disease affected fish species such as Labeo rohita, Cyprinus carpio, H. molitrix, and Puntius sarana from different carp farms and market sources. Bacterial isolates were collected from different tissues or organ such as skin, gill, spot lesions on the skin, abdomen, kidney etc. Several tests were c...
Pseudomonas sp is a gram-negative, rod shape bacterium belonging to the family Pseudomonadaceae. The species is highly adaptable opportunistic pathogen, capable of surviving in a variety of environment, including aquaculture environment. Antibiotics are used in the aquaculture environment, and their improper usage poses a risk of potential transfer...
This study was intended to address, the effect of oxytetracycline, erythromycin, and streptomycin on the antimicrobial resistance of Aeromonas sp. isolated during the production process, from infected common carp (Cyprinous carpio). Antimicrobial resistance patterns were defined by determination of minimal inhibitory concentrations (MIC) of three a...
Seven fresh bacterial isolates were isolated from disease affected fish species such as Labeo rohita, Cyprinus carpio, H. molitrix, and Puntius sarana from different carp farms and market sources. Bacterial isolates were collected from different tissues or organ such as skin, gill, spot lesions on the skin, abdomen, kidney etc. Several tests were c...
Pseudomonas sp is a gram-negative, rod shape bacterium belonging to the family Pseudomonadaceae. The species is highly adaptable opportunistic pathogen, capable of surviving in a variety of environment, including aquaculture environment. Antibiotics are used in the aquaculture environment, and their improper usage poses a risk of potential transfer...
The present study determined the protection afforded by dietary administration with Azadirachta indica extract in terms of mortality and innate immune response to Aeromonas hydrophila infection in silver carp, Hypophthalmichthys molitrix after parasitic infestation in the aquarium. The silver carp was fed with 0% (control), 1.0%, 2.0% and 3.0% supp...
The present study determined the protection afforded by dietary administration with Azadirachta indica extract in terms of mortality and innate immune response to Aeromonas hydrophila infection in silver carp, Hypophthalmichthys molitrix after parasitic infestation in the aquarium. The silver carp was fed with 0% (control), 1.0%, 2.0% and 3.0% supp...
Aims: The study was conducted to examine the efficacy of dietery black cumin seed oil (Nigella
sativa) on the immune response of climbing perch, Anabas testudineus against A. hydrophila.
Place and Duration: This experiment was performed in the Laboratory of Fisheries and Marine
Bioscience (FMB), Jessore University of Science and Technology (JUST),...
The study was carried out to know the present status of Macrobrachium rosenbergii culture in Bagerhat district, Bangladesh from March 2012 to January 2013. Education levels of farmers were found as illiterate (12.3%), primary (36.19%), secondary (20%), SSC (13.33%), HSC (12.38%) and graduate (5.71%). M.