Md. Eaqub Ali

Md. Eaqub Ali
University of Malaya | UM · Centre for Research in Nanotechnology and Catalysis (NANOCEN)

Doctor of Philosophy

About

171
Publications
95,650
Reads
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4,687
Citations
Additional affiliations
March 2012 - September 2016
University of Malaya
Position
  • Professor (Associate)

Publications

Publications (171)
Article
Low DNA concentration recovered from highly processed products such as gelatin and gelatin-based products renders difficulty in detecting porcine contamination using conventional PCR techniques. We documented here a porcine-specific loop-mediated isothermal amplification (LAMP) to identify porcine traces in gelatin products. The porcine-specific pr...
Article
Full-text available
The significance of total polyaromatic hydrocarbons (TPAH) determination in assessing the carcinogenicity of environmental samples for measuring the level of environmental pollution cannot be overemphasized. Despite the environmental danger of TPAH, its laboratory quantification is laborious, which consumes appreciable time and other valuable resou...
Article
Detection of animal materials in gelatin-based products is required to address religious and cultural concerns, because porcine and bovine gelatins are prohibited in Halal, Kosher and Hindus consumer goods. In this paper, multiplex quantitative polymerase chain reaction (qPCR) assay using TaqMan probe was developed to discriminate bovine, porcine a...
Article
Full-text available
Cat, rabbit, rat, and squirrel species are very sensitive in food products because most of them are potential carriers of zoonotic diseases and rejected in most religions and cultures. Since cats and rats are abundant in most parts of the world and their meats do not carry any value in legal markets, these meats could be considered as potential adu...
Article
Species authentication of meat and fish products is crucial to safeguard public health, economic and religious sanctity. We developed a heptaplex PCR (h-PCR) assay targeting short amplicon length (73–198 bp) for the simultaneous detection and differentiation of cow, buffalo, chicken, cat, dog, pig, and fish species in raw and processed food using s...
Article
Full-text available
Objective: The objective of this study was to employ real-time or quantitative polymerase chain reaction (q-PCR) using novel species specific primer (SSP) targeting on mitochondrial cytochrome-b of wild boar species (CYTBWB2-wb) gene for the identification of non-halal meat of wild boar meat (WBM) in meatball products. Materials and methods: The...
Article
Agglomeration is a major setback in the use of pristine multiwall carbon nanotube for many applications. Therefore, application of hydroxyl functionalized multiwall carbon nanotubes (MWCNT-OH) for the removal of naphthalene and fluorine was investigated and compared with pristine MWCNT for the first time. FTIR, BET, TGA, SEM, and TEM were used to i...
Article
Consumption and exploitation of crocodiles have been rampant for their exotic, nutritive and medicinal attributes. These depredations are alarming and although they have continued to be monitored by wildlife and conservation agencies, unlawful trading of crocodiles shows an increasing trend worldwide. Recently, conventional polymerase chain reactio...
Article
Mislabelling in fish products is a highly significant emerging issue in world fish trade in terms of health and economic concerns. DNA barcoding is an efficient sequencing-based tool for detecting fish species substitution but due to DNA degradation, it is in many cases difficult to amplify PCR products of the full-length barcode marker (~650 bp),...
Article
Full-text available
Isothermal amplification is a technique that can amplify target DNA sequences at a single incubation temperature. Loop-mediated isothermal amplification (LAMP) is an extension of the isothermal DNA amplification technique that combines rapidity, simplicity, high specificity and sensitivity. Due to its overwhelming characteristics, LAMP has been exp...
Article
Polycyclic aromatic hydrocarbons (PAHs) that increasingly emanating from natural and human activity sources are invisible pollutants and the presence of even minute amounts of these substances makes them undesirable due to their negative attributes. The PAHs extraction and removal from effluents are major steps for analysis and removal. Chemical, b...
Article
A feeding trial was conducted to evaluate the effects of different ratios of fish meal (FM) to a fermented blend (FB: fermented soybean meal and squid by-product blend) on growth and economic performance of climbing perch, Anabas testudineus (Bloch, 1792), in earthen ponds. Five diets were prepared where FB was substituted for 0 (D1), 25 (D2), 50 (...
Book
Preparation and Processing of Religious and Cultural Foods covers the production and processing of foods from major religions, focusing on the intersection of religion, science and cultural perceptions in the production and processing of modern religious and vegetarian foods. Quality control and authentication technologies are looked at in-depth, w...
Article
Gelatin is widely used in pharmaceuticals as a protective coating, such as soft and hard capsule shells. However, the animal source of gelatin is a sensitive issue because certain gelatins such as porcine and bovine gelatins are not welcome in Halal, Kosher and Hindus’ consumer goods. Recently, we have documented DNA barcoding and multiplex PCR pla...
Article
Full-text available
The objectives of this study are to optimize the extraction of fat from bambangan kernel using response surface methodology and to determine its antioxidant activities and total phenolic contents (TPC). Bambangan kernel was also investigated to determine proximate and amino acids in a pursuit to identify an innovative fruit which could be exploited...
Article
Full-text available
This study was investigated to examine supplemental effects of dietary inosine monophosphate (IMP) on growth performance, feed utilization, biochemical, hematological and immunological parameters of juvenile Nile tilapia Oreochromis niloticus. Disease resistance to experimental infection with Streptococcus agalactiae was also assessed. A semi-purif...
Article
Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus are the most notorious fish borne-pathogens responsible for several deadly diseases in human. However, the prevalence of these three pathogenic vibrios in Malaysian fishes has not been thoroughly investigated, leaving a study gap for the tracing of possible Vibrio outbreak through carri...
Article
Gelatin is widely used in many confectionery products such as gummy, candies, pastilles and marshmallows. However, the acceptance of gelatin in consumer goods largely depends on its animal origins. The main sources of gelatin are pig, cow and fish collagens that have differential acceptance in Halal, Kosher and Hindu foods. Currently, several simpl...
Article
Full-text available
Crocodiles have been hunted and consumed for centuries for skins, nutrients, and medicines. These indomitable trends have overpowered restrictions from wildlife and conservation agencies, continuing the illegal trades of crocodiles across the world. This paper described the development of a very stable, fast, and secured polymerase chain reaction-r...
Article
Full-text available
The aim of the study was to isolate digestive enzymes inhibitors from Mimosa pudica through a bioassay-guided fractionation approach. Repeated silica gel and sephadex LH 20 column chromatographies of bioactive fractions afforded stigmasterol, quercetin and avicularin as digestive enzymes inhibitors whose IC50 values as compared to acarbose (351.02...
Article
Species substitution, the use of a low value fish in place of a high value fish, is the biggest problem in international trade and the leading cause of fraud in the fisheries arena sector. Current DNA barcoding systems have partly solved this problem but also failed in many instances to amplify PCR targets from highly processed products because of...
Article
Full-text available
Supercritical fluid extraction (SFE) using carbon dioxide (CO2) and liquid CO2 using Soxhlet (CO2-Soxhlet) extraction were employed to extract three (3) antidiabetic compounds viz. stigmasterol, quercetin and avicularin from Mimosa pudica. Various extraction parameters were studied. Extracts were analyzed pharmacologically, qualitatively and quanti...
Article
Rabbit meat is receiving increasing attention because it contains more proteins and less fat. On the other hand, squirrel meat is served in aristocrat meals in certain countries and so it is sold at higher prices. The other side of the coin is rat meat that has family ties with rabbit and squirrel but possesses huge threats to public health because...
Article
The demands for rabbit meat are rapidly growing and Rabbitry is becoming a mean of livelihood for many youths. Rats and squirrels are very close relatives of rabbits, could be hunted freely or raised in domestic farming and so could be substituted in expensive rabbit meat. This study, for the first time, developed and validated a tetraplex polymera...
Article
In this study, mango seed fat (MSF) and its recommended blends were stored under accelerated condition. During the accelerated storage, the changes of fatty acids, total phenolic, tocopherol, and phytosterol contents, iodine, free fatty acid (FFA), and peroxide values were examined every six days. Results upon storage, palmitic and stearic acids in...
Article
Cattle, buffalo, and porcine materials are widely adulterated, and their quantification might safeguard health, religious, economic, and social sanctity. Recently, conventional polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism (RFLP) assays have been documented but they are just suitable for identification, cannot qua...
Article
Activated carbon (RSAC) was prepared using the two steps of hydrothermal carbonization (HTC) and chemical activation from the sawdust of Calamus gracilis, commonly known as Rattan (RS). The HTC process was carried out using a Teflon-lined autoclave to produce hydrochar, followed by chemical activation using phosphoric acid (H3PO4). The highest remo...
Article
Full-text available
The effect of temperature on the physiochemical characteristics of a solid fuel or biocoal derived from the dried trunk of Adansonia digitata (Baobab) was studied using torrefaction processes. The chemical composition of the solid fuel or char obtained by wet (HTC) and dry torrefaction processes were determined by elemental and thermogravimetric (T...
Article
Full-text available
Mud crab (Scylla sp.) aquaculture has gained in popularity due to diminishing wild stocks and high market demand. However, the industry is facing a shortage in quality formulated feed and research on practical feed development remains scarce. We conducted a 2×2 factorial experiment to examine the interactive effects of different levels of fishmeal...
Chapter
Detection of specific nucleic acid targets is of enormous and increasing interest in molecular biodiagnostics, food analysis, forensic investigation, and environmental monitoring. The advancement of nanotechnology with a myriad of novel and engineered nanomaterials and their hybrids with attractive physiochemical properties has raised hopes for the...
Article
Gelatin is a highly purified animal protein of pig, cow and fish origins and is extensively used in food, pharmaceuticals and personal care products. However, the acceptability of gelatin products greatly depends on the animal sources of the gelatin. Porcine and bovine gelatins have attractive features but limited acceptance because of religious pr...
Article
Subcritical carbon dioxide Soxhlet extraction of biologically active compounds from Clincanthus nutans was investigated by full factorial design to identify and optimize the factors (particle size and co-solvent) affecting extract yield, antioxidant activity, total phenolic content, total flavonoid content, and α-glucosidase inhibitory activity. An...
Article
Full-text available
Distinctly active poly(hydroxamic acid) anchored copper (Cu) catalysts were synthesized from chemically modified kenaf cellulose. They were characterized by Fourier transform infrared spectroscopy (FT-IR), field emission scanning microscopy (FESEM), high resolution transmission electron microscopy (HRTEM), inductively coupled plasma atomic emission...
Article
Replacement of beef by buffalo and vice versa is frequent in global markets, but their authentication is challenging in processed foods due to the fragmentation of most biomarkers including DNA. The shortening of target sequences through use of two target sites might ameliorate assay reliability because it is highly unlikely that both targets will...
Article
Full-text available
Although many methods have been documented for carbon nanotube (CNT) synthesis, still, we notice many arguments, criticisms, and appeals for its optimization and process control. Industrial grade CNT production is urgent such that invention of novel methods and engineering principles for large-scale synthesis are needed. Here, we comprehensively re...
Article
Full-text available
Phaleria macrocarpa, known as “Mahkota Dewa”, is a widely used medicinal plant in Malaysia. This study focused on the characterization of α-glucosidase inhibitory activity of P. macrocarpa extracts using Fourier transform infrared spectroscopy (FTIR)-based metabolomics. P. macrocarpa and its extracts contain thousands of compounds having synergisti...
Article
Muscle growth mechanisms are controlled by different cellular and molecular pathways, and dietary supplementation of nucleotides (NTs) may play an important role to influence such pathways in fish. In this study, we hypothesized that dietary supplementation of inosine monophosphate (IMP) would enhance cellular muscle growth, and upregulate growth-r...
Article
Full-text available
The Malayan box turtle (Cuora amboinensis) (MBT) is a vulnerable and protected turtle species, but it is a lucrative item in the illegal wildlife trade because of its great appeal as an exotic food item and in traditional medicine. Although several polymerase chain reaction (PCR) assays to identify MBT by various routes have been documented, their...
Data
Pairwise distances of the Malayan box turtle (MBT)-specific primer sites of the cytochrome b gene against the corresponding sites of 29 different species. (PDF)
Data
Evolutionary distances between Malayan box turtle and other reptiles. 1–44: turtles/tortoises; 48–57: snakes; 58–65: crocodiles; and 45–47 and 66–67: lizards. Phylogenetic tree was constructed using the Malayan box turtle-specific 120-bp site of the cytochrome b gene against 66 reptile species, of which 44 were turtle (1–44), 10 were snake (48–57),...
Poster
Perkembangan pesat teknologi pemprosesan makanan telah menimbulkan pelbagai isu yang berkaitan dengan halal dan haram sehingga dapat memberi kegusarankepadapara penggunaterutamanyapenggunaMuslim. Misalnya, pelbagai bentuk pemalsuan dan penipuan dilakukan oleh pengeluar untuk mengurangkan kos di samping untuk mengoptimumkan keuntungan. Misalnya sepe...
Article
Full-text available
Activated carbon was produced by chemical activation of hydrothermally carbonized (HTC) banana empty fruit bunch (BEFB), using phosphoric acid (H3PO4) as the activating agent. The activation process was optimized using a Box-Behnken factorial design (BBD), with an outcome of 17 different experiments under the predefined conditions. Three different...
Article
The Malayan box turtle (Cuora amboinensis) (MBT) is a vulnerable and protected turtle species widely used in exotic foods and traditional medicines. Currently available polymerase chain reaction assays to identify MBT lack automation and involve long targets which break down in processed or denatured tissue. This SYBR Green duplex real time PCR ass...
Article
Full-text available
Benzyl α-L-rhamnopyranoside 4, obtained by both conventional and microwave assisted glycosidation techniques, was subjected to 2,3-O-isopropylidene protection to yield compound 5 which on benzoylation and subsequent deprotection of isopropylidene group gave the desired 4-O-benzoylrhamnopyranoside 7 in reasonable yield. Di-O-acetyl derivative of ben...
Article
Beef, buffalo and pork are the major meat of economic, religious and health concern. Current methods to authenticate these materials in food chain are based on mainly single gene targets which are susceptible to break down by food processing treatments. We, for the first time, described here a double gene targeting short-amplicon length multiplex p...
Article
Beef, buffalo and pork adulteration in food chain is an emerging and sensitive issue. Current molecular techniques to authenticate these species depend on PCR assays involving long and single targets which break down under natural decomposition and/or processing treatments. This novel PCR-RFLP assay targeted two different gene-sites for each of the...
Article
Full-text available
A free radical chain initiation reaction was exploited to prepare poly(acrylonitrile)-grafted Khaya cellulose. The synthesis of the poly(amidoxime) ligand was also performed using oximation reactions. Transition metal cations formed some complexes with the polymeric ligand. The pH of the solution played an important role in the optical detection of...
Article
Full-text available
This research emphasizes the isolation of nanocrystalline cellulose (NCC) from palm tree cellulose (PTC) and α-cellulose (AC), using acidic FeCl3-assisted catalytic pretreatment coupled with ultrasonication. The cavitation effect of ultrasonication affects the microstructure of the fibers, ultimately enhancing the crystallinity index of the prepare...
Article
Background: Poor quality and quantity of DNA extracted from gelatin and gelatin capsule often cause failure in animal species determination using PCR. Gelatin, which is mainly derived from porcine and bovine, has been a matter of concern among customers in order to fulfill religious obligation and safety precaution against several transmissible in...
Article
An identification method of lard in chocolates using real-time polymerase chain reaction was developed to address Halal authentication. However, polymerase chain reaction detection of lard in chocolate has been in vain. In order to investigate the inhibitory effect exerted by each of the chocolate components, four basic chocolate components, sugar,...
Article
Full-text available
This research emphasizes the isolation of nanocrystalline cellulose (NCC) from palm tree cellulose (PTC) and α-cellulose (AC), using acidic FeCl3-assisted catalytic pretreatment coupled with ultrasonication. The cavitation effect of ultrasonication affects the microstructure of the fibers, ultimately enhancing the crystallinity index of the prepare...
Article
The development of an environmentally benign process for extraction of valued chemicals by catalytic hydrolysis of cellulose is considered as one of the key technologies for making complete usage of cellulosic biomacromolecules in near future. Present research deals with the application of Lewis acid of iron(III) chloride (FeCl3) based catalytic hy...
Article
The macaque (Macaca fascicularis) monkeys are the third-largest primate population in tropical forests. Being the potential carrier of Simian Immunodeficiency, Ebola and Corona viruses as well as religious and wildlife protected species could not saved macaques from being over hunted and consumed. Despite having needs, methods to detect monkey spec...
Article
Full-text available
Clean and safe water crises have become one of the major global problems for decades. To address this issue, various water purification technologies have been adopted. Conventional water purification technologies are time consuming, expensive, and have low affinity and efficiency to newly emerging micropollutants in water. The paradigm might compel...
Article
Hydrophobic carbon nanotubes aggregate and precipitate in polar solvents. These make their handling difficult and limit their applications in various fields including water purification technologies, catalysis, polymers, composites, sensors and optoelectronics. Here we report two covalent functionalization schemes for multi-walled carbon nanotubes...
Article
Full-text available
Adulteration of meat in processed food is a sensitive issue since certain meat species are prohibited in some religions such as Islam and Judaism. Some meat types are also potential carrier of some deadly diseases such as sars, anthrax, and hepatitis. Furthermore, unconscious consumption of certain meat might lead to an allergic reaction. Feline me...
Article
Full-text available
Here we describe a short-amplicon based TaqMan probe quantitative real-time PCR (qPCR) assay for the quantitative detection of canine meat in chicken nuggets which are very popular across the world including Malaysia. The assay targeted a 100-bp fragment of canine cytb gene using a canine specific primer and TaqMan probe. Specificity against 10 dif...
Article
Full-text available
Food forgery has posed considerable risk to public health, religious rituals, personal budget and wildlife. Pig, dog, cat, rat and monkey meat are restricted in most religions, but their sporadic adulteration are rampant. Market controllers need a low-cost, but reliable technique to track and trace suspected species in the food chain. Considering t...