Md Abdul KafiBangladesh Agricultural University | BAU · Department of Microbiology and Hygiene
Md Abdul Kafi
DVM & MS (BAU), PhD (Sogang), JSPS Fellow (Kyushu) & Marie Curie Individual Fellow (Glasgow)
Looking for collaborators, researchers, fellows interested on Nanobiomaterial, Biosensor and Tissue engineering research
About
55
Publications
15,107
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Introduction
Md Abdul Kafi received DVM and MS in Microbiology from BAU, Bangladesh. Then, he pursued his PhD from Sogang University, Korea. During PhD research, Dr. Kafi developed cell based sensor environmental monitoring with level free electrochemical methods. Afterwards, he was awarded with Japanese Society for the Promotion of Science (JSPS) fellowship for working at Kyushu University, Japan. Where, he developed peptide functionalized chitosan scaffold suitable for hMSC adhesion and proliferation. Now, Dr. Kafi is working as a Marie Curie Individual fellow at University of Glasgow, UK. He is working on BIOPOLYMER based platform for biocompatible, biodegradable and bioresorbable electronics for wearable/implantable applications.
Additional affiliations
August 2016 - August 2018
August 2015 - present
November 2012 - November 2014
Education
August 2007 - August 2011
January 2002 - June 2003
August 1996 - September 2001
Publications
Publications (55)
Extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli cause severe health hazards. Migratory birds are reservoirs and transmitters of many pathogens including ESBL-producing E. coli. To examine migratory birds as potential carriers of ESBL-producing E. coli and E. coli-carrying antibiotic resistance genes, 55 PCR-positive E. coli isola...
Battle with the adverse impact of climate change on the agricultural productivity and susceptibility of the crops is of premier importance for ensuring food security and sustained agricultural developments. The agro-nanotech innovations offer "low input but maximum output" based agro-farming are well aligned with the desired crops production. This...
The rapid emergence of virulent and multidrug-resistant (MDR) non typhoidal Salmonella(NTS) enterica serovars is a growing public health concern globally. The present study focused on the assessment of the pathogenicity and antimicrobial resistance (AMR) profiling of NTS enterica serovars isolated from the chicken processing environments at wet mar...
The rapid emergence of virulent and multidrug-resistant (MDR) non-typhoidal Salmonella (NTS) enterica serovars are a growing public health concern globally. The present study focused on the assessment of the pathogenicity and antimicrobial resistance (AMR) profiling of NTS enterica serovars isolated from chicken processing environments at wet marke...
This research presents the application of glycine amino acid for establishing firm cell-substrate interaction instead of expensive adhesion proteins, peptides and peptide derivatives. The glycine amino acid is chemically functionalized on the coverslip to achieve self-assembled nanostructure. Glycine self-assembly on NaCl treated coverslips is init...
Migratory birds are carriers of multidrug resistant pathogenic Escherichia coli. However, their roles in the dissemination of these resistant pathogens are still being neglected in Bangladesh. The present study was therefore carried out to detect multidrug resistant E. coli. In addition, these isolates were also screened for the presence of avian p...
An antibiogram study of newly introduced Silver Nano particles (AgNPs) was performed against the available poultry bacteria. Herein, this research involved isolation, identification and characterization of Staphylococcus, E. coli and Salmonella of different bird types from two live bird markets surrounding Bangladesh Agricultural University (BAU) c...
This research involved synthesis and characterization of
Silver Nano particle (AgNP) for using as antibacterial
agent. AgNP was achieved from Silver Nitrate reduced
with Sodium Tripolyphosphate. The synthesized AgNP
were characterized with AFM and SEM. The morphological investigations revealed 100- 150nm sized particles
homogenously from the yielde...
Cellular electrophysiological state is sensitive to any adverse external stimuli such as changes in nutrient, and presence of toxicants, heavy metals, and infectious agents. Based on the issues and promises related to cell‐based sensing devices, this chapter discusses the state‐of‐the‐art cell chip design and fabrication, substrate functionalizatio...
Background and Aim: Houseflies (Musca domestica) are synanthropic insects which serve as biological or mechanical
vectors for spreading multidrug-resistant bacteria responsible for many infectious diseases. This study aimed to detect
antibiotic-resistant bacteria from houseflies, and to examine their resistance genes.
Materials and Methods: A total...
In vitro tissue engineering requires a progenitor cell source and a porous scaffold providing three dimensional (3D) supports for growth and differentiation to attain tissue architectures. This research focused on fabrication and characterization of 3D porous scaffolds using chitosan (CS), collagen (CG) and chitosan-collagen (CS-CG) composite to in...
Scaffold engineering has attracted significant attention for three-dimensional (3D) growth, proliferation and differentiation of stem cells in vitro. Currently available scaffolds suffer from issues such as poor ability for cell adhesion, migration and proliferation. This paper addresses these issues with 3D porous chitosan scaffold, fabricated and...
This work presents a Chitosan - Graphene Oxide (CS-GO) based array of ultra-thin biosensors with gold (Au) based microgap (60μm) electrode. The cross-linked GO is shown to improve the stability of chitosan substrate in aqueous medium and compatibility with microfabrication steps. The sensor patch has been evaluated for label free monitoring by immo...
This research demonstrates fluctuation of glutathione peroxidase1 (Gpx1) throughout cell cycle progression with significant decreased expression at mitosis of HeLa cell. This was achieved with western blot (WB) analysis of target proteins from each phase of synchronized cells. The synchronizations were performed with double thymidine (T/T) for G1/S...
his paper reports a Graphene Oxide (GO)-Chitosan based chemiresistor for in situ label-free electrochemical detection of serotonin. This ultra-flexible sensor consists of platinum (Pt) electrodes, separated by a 60 µm gap, patterned on 2.5 µm thick GO-Chitosan film which acts as the active sensing material. Due to its low film thickness, our propos...
An in vitro virus infectivity assay based on the newly introduced spectrochemical method was performed using Peste des Petits Ruminants (PPR) virus infected Vero cell line as a model infection. Herein, the mitochondrial reductase enzyme activity was monitored using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reagent as a subs...
Biomaterials having cell adhesion ability are considered to be integral part of a cell chip. A number of researches have been carried out to search for a suitable material for effective immobilization of cell on substrate. Engineered ECM materials or their components like collagen, Poly-l-Lysine (PLL), Arg-Gly-Asp (RGD) peptide have been extensivel...
Avian influenza viruses (AIVs) continue to pose a global threat. Waterfowl are the main reservoir and are responsible for the spillover of AIVs to other hosts. This study was conducted as part of routine surveillance activities in Bangladesh and it reports on the serological and molecular detection of H5N1 AIV subtype. A total of 2169 cloacal and 2...
Development of a rapid, sensitive and cost-effective method for toxicity assessment of commonly used nanoparticles is urgently needed for the sustainable development of nanotechnology. A neural cell with high sensitivity and conductivity has become a potential candidate for a cell chip to investigate toxicity of environmental influences. A neural c...
Calcium phosphate bioceramics such as hydroxyapatite (HA) have widely been applied as scaffolds in bone tissue engineering because of high osteo-conductivity and biocompatibility. In the present study, continuous porous HA scaffold was fabricated using the template method. Human mesenchymal stem cells (hMSC) were then seeded into the HA scaffold up...
Fabrications of biodegradable polymer materials are of much significance in establishing living cells on artificial surfaces for tissue engineering applications. Chitosan derived by de-acetylation of chitin may be one of the important candidates for this purpose. In the present study, porous chitosan scaffolds were fabricated using freezing (-20°c...
A cell-based chip was recently developed and shown to be an effective in vitro tool for analyzing effect of environmental toxin on target cells. However, common cell chips are inappropriate for the detection of multiple environmental toxins. Here, we fabricated a neural cell chip to detect different cellular responses induced by BPA (bisphenol-A) a...
The present study estimated the efficacy of electrochemical detection of imidazolidinyl urea-induced cell toxicity in skin human fibroblast cells (HFF cells). The gold nanopunct structures were fabricated through a nanoporous alumina mask, and the structural formations were confirmed via scanning electron microscopy. The HFF cells were allowed to a...
In this paper, a method was introduced for the fabrication of vertically and spatially-controlled peptide nanostructures that enhance cell adhesion, proliferation, spreading on artificial surfaces. The RGD nanostructures with different heights were fabricated on gold surfaces by self-assembly technique through a nanoporous alumina mask composed of...
Cell chip was fabricated to analyze different cell lines and cell cycle-stages based on the electrochemical tools. Cell-based biochips are becoming promising tools in the various kinds of biotechnology field such as early diagnosis, cell therapy and drug screening. In this study, cells were immobilized on collagen coated gold surface and subjected...
A cell chip with a nano-scaled thin film of cysteine modified synthetic oligopeptide C(RGD)4 was fabricated to detect dopamine secretion from neuronal cells. Thin C(RGD)4 peptide layer was fabricated on chip surface for increasing the binding affinity of cells to gold electrode surface, which is essential for the electrochemical detection of dopami...
A cell-based chip was fabricated for in vitro label-free monitoring of dopamine secreted from neural cells. PC12 cells were grown on the chip and allowed for cyclic voltammetric measurement to detect dopamine secretion. A quasireversible redox process was successfully observed from the cellular dopamine, and the voltammetric results showed the posi...
A new strategy for on-site monitoring of cell cycle progression was proposed using cell chip technology. Cell synchronization has been utilized in intensive cellular research due to the fact that cells in different phases of the cell cycle exhibit different behaviors even when exposed to the same concentrations of drugs or toxicants. However, confi...
A cysteine-terminated C(RGD)(4) peptide film was fabricated on a gold electrode for improving the attachment of cells. The electrochemical signals of cyclic voltammogram from cells on a C(RGD)(4) deposited electrode was enhanced from 0.27 to 0.49 μA compared to a bare electrode. The developed cell-based sensor determined the effect of bisphenol-A (...
The investigation was conducted to determine the persistence of maternally derived antibody as well as its influence on vaccination programme against fowl pox infection. A total of 60-day-old chicks divided into four groups such as A, B, C, D. Chicks of Group A and B were used for the detection of persistence of maternally derived antibody which ar...
Efficacy of experimentally prepared formalin killed fowl cholera vaccine in Fayoumi chickens via different routes of vaccination was determined during the period from April 2002 to March 2003. Pasteurella multocida (PM-38) serotype 1 (X-73) was employed for vaccine preparation and antibody titres of the chicken sera were determined by passive haema...
The peste des petits ruminants (PPR) has now been recognized as endemic viral disease associated with high morbidity and mortality rates in goats of Bangladesh. As there is no known effective drug against viral diseases, an attempt was made to evaluate the efficacy of antibiotic combined hyperimmune serum therapy (ACHST) against the deadly viral di...
The serological responses and protection of Sonali breed chickens with Australian Newcastle disease V4 heat resistant (NDV4HR) live freeze-dried vaccine (Australian Webster Pvt. Ltd., Sydney) was compared with that of locally produced conventional Lentogenic F-strain Baby Chick Ranikhet Disease vaccine (BCRDV, DLS, Dhaka) of Bangladesh. Thirty day-...
The study was undertaken during the period from July to November 2005, for the first time, to determine the efficacy of thermostable PPR vaccine incubated at -20 0 C and room temperature (RT) for 14 days which was developed by Bangladesh Livestock Research Institute (BLRI), Savar, Dhaka, in collaboration with and Livestock Research Institute (LRI),...
In an investigation of Newcastle disease (ND) vaccination programme, the performance of ND killed vaccine as a secondary one was performed to examine duration and level of antibody in both broiler and layer breeder parent stock in the Department of Microbiology and Hygiene, BAU, Mymensingh during the period from January to April 2004. For this, a t...
Persistence of maternally derived antibody to Baby Chick Ranikhet Disease Vaccine and its influence on vaccination programme in chicks of vaccinated and nonvaccinated origin were determined during the period from July 2005 to May 2006. The study suggested an effective vaccination schedule of Baby Chick Ranikhet Disease Vaccine in chicks of vaccinat...
Questions
Questions (2)
I am Looking for suitable centrifugal speed for separation nano-particles from bulk solution,
Can any one advise me the way of in situ monitoring of redox couples during a voltametric analysis?
Projects
Projects (6)
Regeneration of Bone tissue In vitro
This work presents a Chitosan - Graphene Oxide (CS-GO) based array of ultra-thin biosensors with gold (Au) based microgap (60μm) electrode. The cross-linked GO is shown to improve the stability of chitosan substrate in aqueous medium and compatibility with microfabrication steps. The sensor patch has been evaluated for label free monitoring by immobilizing the CS-GO surface with human dermal fibroblast (HDF) cells. The cyclic voltammetry (CV) of HDF cell immobilized CS-GO surface show quasi-reversible nature with characteristic cathodic peak at 300mV and anodic peak at +300mV. Both peaks are stable and repeatable up to 50-scan cycle without any potential shift. The device shows steady state peak enhancement (1.923-11.195nA) during the DHF cell growth period (0-96h). The redox peak enhancement correlates with the cell proliferation rates over time, indicating that it could be employed for investigation of cyto-physiological state against any endo and exogenous stimulation. In addition, the developed sensor-patch was used to detect a wide range of glucose from 1μM to 20mM in vitro with a sensitivity of 0.17μA/mM. Considering these, the presented sensor-patch has a great potential for the detection of glucose level, cell-health proliferation rate at the wound site and diabetic wound monitoring applications.
Strengthening small-scale livestock farming