Mazhar I Khan

Mazhar I Khan
University of Connecticut | UConn · Department of Pathobiology and Institute of Material Science

DVM, MPVM, PhD

About

238
Publications
37,322
Reads
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2,671
Citations
Citations since 2017
21 Research Items
1051 Citations
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2017201820192020202120222023050100150
2017201820192020202120222023050100150
Introduction
Mazhar I Khan currently works at the Department of Pathobiology and Institute of Material Science, University of Connecticut. Mazhar does research in Bioinformatics, Biostatistics and Biotechnology. Their current project is 'Immunogenicity Studies on Nanoparticle Based Vaccines for Bird Flu'.
Additional affiliations
September 1989 - present
University of Connecticut
Position
  • Professor (Full)
September 1989 - present
University of Connecticut
Position
  • Professor (Full)
Description
  • Comparative Pathology, Pathobiology of Avian Species
Education
September 1982 - December 1987
University of California, Davis
Field of study
  • Molecular Epidemiology, Comparative Pathology

Publications

Publications (238)
Article
Full-text available
Significance Bacteria can produce β-lactamases, enzymes that destroy β-lactam antibiotics and thereby resist these potent antibiotics that target cell wall synthesis. Production of β-lactamases is often controlled by β-lactam-induced perturbations in the cell wall. Here, we have identified a new mechanism controlling β-lactamase production. We foun...
Article
Full-text available
Undergraduate research (UR) is a high-impact practice (HIP) to engage undergraduate student in science, technology, engineering and mathematics (STEM), especially from underrepresented groups. UR experiences (UREs) can be integrated into the classroom, making authentic research experiences inclusive and available to all students. However, developin...
Article
Full-text available
Zika, yellow fever and dengue viruses are three important flaviviruses that are epidemic and endemic in Central Africa, Middle and South America, Southeast Asia and Pacific Islands. Since these three viral diseases present with similar symptoms and there is a possibility of multiple flaviviruses co-infection, it is important to develop an effective...
Article
Full-text available
Most of the killed, inactivated, or live-attenuated pathogen vaccines are now replaced by modern vaccines containing isolated, highly purified antigenic protein subunits which are safer than live viruses or deactivated viruses. Another strategy is the development of nanoparticles which can mimic the repetitiveness, geometry, size and shape of the h...
Article
Full-text available
Background: Infectious bursal disease virus (IBDV) is the causative agent of Infectious Bursal Disease (IBD), the disease causes immunosuppression which leads to secondary infections among rearing poultry flocks. Characterization of the virus is important for its control and eradication. The circulating IBDVs are classified on the basis of their a...
Article
Full-text available
The objective of this study was to identify the species and characterize the genetic relationships among mycoplasma isolates from commercial layer hen flocks using 16S-23S rDNA intergenic spacer region (IGSR) sequencing. Twenty-one isolates were obtained from samples collected from commercial layer flocks in four Brazilian states: São Paulo, Minas...
Preprint
Full-text available
Article
Full-text available
Vaccines are one of the most outstanding innovations in public health. They save millions of lives by the prevention of infectious diseases. Throughout the past two centuries, most vaccines have been formulated by live or killed whole pathogens. Live vaccines have a safety issue that is a risk of reversing back to their virulent state. Kill vaccine...
Article
Full-text available
Infectious bronchitis virus (IBV) affects poultry respiratory, renal and reproductive systems. Currently the efficacy of available live attenuated or killed vaccines against IBV has been challenged. We designed a novel IBV vaccine alternative using a highly innovative platform called Self-Assembling Protein Nanoparticle (SAPN). In this vaccine, B c...
Data
Amino acid sequence of each SAPN monomer. (A) IBV-SAPN monomer (Monomer A). (B) Flagellin-SAPN monomer (Monomer B); Color scheme: Red, malaria protein; Green: a pentameric coiled coil; yellow: trimeric coiled coil; Blue: HR2 B cell epitope; Black: linker sequences; Pink: CD4 T cell epitopes; Purple: Sequence of the flagellin D0 and D1 domains. (TIF...
Data
SDS-PAGE analysis of assembled SAPNs. 1- IBV-SAPN monomer (Monomer A, Mw 37.8 kDa); 2- IBV-Flagellin-SAPN assembled from Monomer A and Monomer B at a 58:2 molar ratio; and 3- Flagellin-SAPN monomer (Monomer B, Mw 64.5 kDa. (TIF)
Article
Full-text available
Vaccines are one of the most outstanding innovations in public health. They save millions of lives by the prevention of infectious diseases. Throughout the past two centuries, most vaccines have been formulated by live or killed whole pathogens. Live vaccines have a safety issue that is a risk of reversing back to their virulent state. Kill vaccine...
Article
Full-text available
Vaccines are one of the most outstanding innovations in public health. They save millions of lives by the prevention of infectious diseases. Throughout the past two centuries, most vaccines have been formulated by live or killed whole pathogens. Live vaccines have a safety issue that is a risk of reversing back to their virulent state. Kill vaccine...
Article
Full-text available
Avian Colibacillosis is among the major causes of economic loss in the poultry industry worldwide, with a more vivid impact on developing countries. The involvement of several bacteria has made it challenging to develop effective vaccines for this disease, particularly because it is notoriously difficult to make a vaccine that contains all the cont...
Data
NC3Rs ARRIVE guidelines checklist. (PDF)
Data
Temperature dependent induction of E-lysis protein expression. (TIF)
Article
Full-text available
We used a platform of self-assembling protein nanoparticles (SAPNs) to develop a self-adjuvanted vaccine for avian influenza virus (AIV). A SAPNs named SA-MC-Penn was designed to display the ectodomain of influenza matrix protein 2 (M2e) as a tetramer and Helix C (Hel C) of hemagglutinin as a trimer. Flagellin domains expressed within a peptide cha...
Article
Full-text available
The poultry industry has emerged as one of the largest and fastest growing public sectors in the developed and developing countries. Unfortunately, this industry is under a major threat from diseases that are viral (Newcastle disease, infectious bursal disease, influenza, hydropericardium syndrome), bacterial (colibacillosis, pasteurellosis, salmon...
Article
Full-text available
The objective of this study was to evaluate the susceptibility of metallo-β-lactamase (MBL)-producing Acinetobacter baumannii (A. baumannii) clinical isolates to biocides. We also determined the prevalence and correlation of efflux pump genes, class 1 integron and MBL encoding genes. In addition, blaVIM, blaNDM-1, qacE and qacEΔ1 nucleotide sequenc...
Article
Full-text available
The World Health Organization has listed H6 and H9 avian influenza viruses (AIVs) for impending pandemic outbreaks. Therefore, a specific, sensitive and rapid method for diagnosing H6 and H9 subtype’s infections is crucial for establishing control strategies after outbreaks. A duplex reverse transcriptase loop-mediated isothermal amplification (dRT...
Article
Full-text available
Current influenza vaccines should be improved by the addition of universal influenza vaccine antigens in order to protect against multiple virus strains. We used our self-assembling protein nanoparticles (SAPNs) to display the two conserved influenza antigens M2e and Helix C in their native oligomerization states. To further improve the immunogenic...
Article
Current influenza vaccines should be improved by the addition of universal influenza vaccine antigens in order protect against multiple virus strains. We used our self-assembling protein nanoparticles (SAPNs) to display the two conserved influenza antigens M2e and Helix C in their native oligomerization states. To further improve the immunogenicity...
Chapter
Viral quasispecies sequences reconstruction by analyzing HTS/Next Generation Sequencing (NGS) data will contribute to understanding the roles and interactions of infected animal and viral variants for improving animal health and productivity. This understanding is necessary for animal health, and production efficiency, which is one of the goals inc...
Chapter
Full-text available
This volume, which contains the contributions of specialists in respective viral pathogen research, summarizes current diagnostic approaches for major animal viral pathogens with a special emphasis on molecular techniques. Each chapter includes a brief review on the classification, epidemiology, clinical features and diagnosis of animal viral patho...
Article
Fowl adenovirus-4 is an infectious agent causing Hydropericardium syndrome in chickens. Adenovirus are non-enveloped virions having linear, double stranded DNA. Viral genome codes for few structural and non structural proteins. 100K is an important non-structural viral protein. Open reading frame for coding sequence of 100K protein was cloned with...
Article
Full-text available
Fowl adenovirus-4 is an infectious agent causing Hydropericardium syndrome in chickens. Adenovirus are non-enveloped virions having linear, double stranded DNA. Viral genome codes for few structural and non structural proteins. 100K is an important non-structural viral protein. Open reading frame for coding sequence of 100K protein was cloned with...
Article
Full-text available
Background The H10 subtype avian influenza viruses (H10N4, H10N5 and H10N7) have been reported to cause disease in mammals, and the first human case of H10N8 subtype avian influenza virus was reported in 2013. Recently, H10 subtype avian influenza viruses (AIVs) have been followed more closely, but routine diagnostic tests are tedious, less sensiti...
Article
Full-text available
One of the serious and growing challenges for infection control programs worldwide is hospital acquired infections. Pseudomonas aeruginosa is considered the most common cause of hospital acquired Gram-negative infections. Intensive exposure of hospital pathogens to biocides may result in the emergence of resistance not only to the biocides, but pos...
Article
Full-text available
Salmonella Enteritidis (SE) is a major foodborne pathogen responsible for causing gastrointestinal infections in humans, predominantly due to the consumption of contaminated eggs. In layer hens, SE colonizes the intestine and migrates to various organs, including the oviduct, thereby leading to egg yolk and shell contamination. This study investiga...
Article
Full-text available
Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) are the most pathogenic and economically important mycoplasma pathogens that infect chickens. The development of rapid and innovative molecular diagnostic techniques is of pivotal importance for their effective control. The aim of the present study was to develop a novel duplex TaqMan real-...
Article
Full-text available
Abstract: Bovine viral diarrhea virus (BVDV) and bovine rotavirus (BRV) are important pathogens of cattle. These pathogenselicitsigns that is sometimes difficult to differentiate. A real-time duplex RT-PCR assay would allow the simultaneous detection of the two viruses in the same sample. A real-time duplex RT-PCR assay was developed and optimized....
Article
Full-text available
Avian infectious bronchitis (IB) is a viral, acute and highly contagious disease caused by infectious bronchitis virus (IBV). The disease affects broilers improvement and performance causing major economic losses in the world poultry industry. Generally, IBV infections can be diagnosed by detection of IBV itself or the specific antibody response. T...
Article
Full-text available
Salmonella Enteritidis (SE) is a major foodborne pathogen in the United States, causing gastroenteritis in humans, primarily through consumption of contaminated eggs. Chickens are the reservoir host of SE. In layer hens, SE colonizes the intestine and migrates to various organs including the oviduct, leading to egg contamination. This study investi...
Article
Full-text available
Fowl adenovirus-4 is an infectious agent causing Hydropericardium syndrome in chickens. Adenovirus are non-enveloped virions having linear, double stranded DNA. Viral genome codes for few structural and non structural proteins. 100K is an important non-structural viral protein. Open reading frame for coding sequence of 100K protein was cloned with...
Article
Recent trials on novel oral anticoagulants (NOAC) in patients undergoing cardioversion showed that NOACs are as safe and effective as treatment with vitamin K antagonists in patients with atrial fibrillation undergoing electric or pharmacological cardioversion. We conducted an EMBASE and MEDLINE search for studies in which patients undergoing cardi...
Conference Paper
Avian influenza (AI) outbreaks pose a significant risk to poultry production in the U.S. and in other countries, human health as well. Type A influenza viruses are typically infecting avian hosts. Control of AI in poultry varies by country and may be based on vaccination and/or surveillance/eradication programs. Limitations exist with the use of li...
Article
Full-text available
We report here the full-length RNA genomic sequence of the bovine viral diarrhea virus (BVDV) strain GX4, isolated from a cow in southern China. Studies indicate that BVDV GX4 belongs to the BVDV-1b subtype. This report will help in understanding the epidemiology and molecular characteristics of BVDV in southern China cattle.
Article
Full-text available
We report here the full-length RNA genomic sequence of the bovine viral diarrhea virus (BVDV) strain GX4, isolated from a cow in southern China. Studies indicate that BVDV GX4 belongs to the BVDV-1b subtype. This report will help in understanding the epidemiology and molecular characteristics of BVDV in southern China cattle.
Article
Full-text available
Avian reovirus (ARV) infection causes considerable economic loss to the commercial poultry industry. Live-attenuated vaccine strain S1133 (v-S1133, derived from parent strain S1133) is considered the safest and most effective vaccine and is currently used worldwide. To identify the genes responsible for its attenuation, DNA sequences of open readin...
Article
Avian reovirus infection causes considerable economic loss to the commercial poultry industry. Live-attenuated vaccine strain S1133 (v-S1133, derived from parent strain S1133) is considered the safest and most effective vaccine and is currently used worldwide. To identify the genes responsible for its attenuation, DNA sequences of open reading fram...
Article
Full-text available
We have previously developed a software package called PrimerHunter to design primers for PCR-based virus subtyping. In this study, 9 pairs of primers were designed with PrimerHunter and successfully used to differentiate the 9 neuraminidase (NA) genes of avian influenza viruses (AIVs) in multiple PCR-based assays. Furthermore, primer pools were de...
Article
Full-text available
A duplex quantitative real-time polymerase chain reaction (dq-PCR) assay was optimized to simultaneously detect Haplosporidium spp. and Perkinsus spp. of shellfish in one reaction. Two sets of specific oligonucleotide primers for Haplosporidium spp. and Perkinsus spp., along with two hydrolysis probes specific for each parasite group, were used in...
Article
Full-text available
Chronic Respiratory Disease (CRD) is caused by Mycoplasmas gallisepticum (MG) and is prevailing in layer, broiler and breeder poultry flocks. The infected birds showed sneezing, rales, coughing and exudates from nostrils and eyes. Mycoplasma species were recovered 27.3% overall, 39.3 per cent of the tracheal swabs, 15.9 per cent of tracheal tissues...
Article
In the present study formaldehyde inactivated Montanide ISA70 based MG vaccine from the PCR confirmed positive local isolate was prepared and evaluated in broilers. An amount of immune gen per 0.3ml of the dose was 107 Colony forming units of the bacteria. At the age of 14 days, the broilers were randomly divided into three groups (A, B and C), eac...
Article
Full-text available
Experiment was conducted to study heterosis for different morphological traits i.e. productive tillers per plant, primary branches per panicle, seeds per panicle, seed weight per panicle, 1000 seed weight, yield per plant and seed length width ratio under different irrigated conditions with Randomized Complete Block Design (RCBD) at University of A...
Article
Full-text available
Infectious bronchitis is caused by a coronavirus, infectious bronchitis virus (IBV). Infectious bronchitis is an acute and highly contagious disease of economic importance due to the reduction in weight gain observed with infected broilers and the drop in egg quality and production associated with infected laying hens. The presence of deep pectoral...
Article
Full-text available
In this study, we investigated the therapeutic efficacy of the plant compounds trans-cinnamaldehyde (TC) and eugenol (EG) on reducing Salmonella enterica serovar Enteritidis (SE) in commercial, market-age broiler chickens. Straight run, day-old commercial broiler chicks (N = 84) were randomly grouped into 6 groups of 14 birds each (n = 14/group): a...