Mayuri Sadoine

Mayuri Sadoine
Heinrich-Heine-Universität Düsseldorf | HHU · Department of Biology

PhD in Molecular Biophysics
Molecular Biophysicist & Microbiologist working on plant-microbe interactions and career development

About

14
Publications
1,495
Reads
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103
Citations
Citations since 2017
13 Research Items
92 Citations
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Introduction
I am watching at the thieves... Pathogens infect host for reproduction, this means in fine nutrition. A very fundamental question is how pathogens feed in the host. As thieves, there are driven by the needs to get valuable goods and to stay invisible to the alarm system. Is there something more challenging than looking at something that wishes to stay hidden?
Additional affiliations
February 2015 - November 2018
RWTH Aachen University
Position
  • PhD Candidate
December 2014 - December 2017
Forschungszentrum Jülich
Position
  • Research Assistant
Description
  • Studying co-translational folding and protein conformation by using single-molecule FRET technics
Education
February 2015 - November 2018
September 2013 - June 2014
Université Libre de Bruxelles
Field of study
  • Master's degree of Public health
September 2009 - June 2011
Université Libre de Bruxelles
Field of study
  • Master's degree Biochemistry, molecular and cellular biology

Publications

Publications (14)
Article
Förster Resonance Energy Transfer (FRET) studies performed at the single molecule level have unique abilities to probe molecular structure, dynamics and function of biological molecules. This technique requires specimens, like proteins, equipped with two different fluorescent probes attached at specific positions within the molecule of interest. He...
Article
Full-text available
Genetically encoded fluorescent sugar sensors are valuable tools for the discovery of transporters and for quantitative monitoring of sugar steady-state levels in intact tissues. Genetically encoded Förster resonance energy-transfer sensors for glucose have been designed and optimized extensively, and a full series of affinity mutants is available...
Article
Full-text available
The understanding of signaling and metabolic processes in multicellular organisms requires knowledge of the spatial dynamics of small molecules and the activities of enzymes, transporters, and other proteins in vivo, as well as biophysical parameters inside cells and across tissues. The cellular distribution of receptors, ligands, and activation st...
Article
Full-text available
Plant hormones play important roles in plant growth and development, physiology and in acclimation to environmental changes. The hormone signaling networks are highly complex and interconnected. It is thus important to not only know where the hormones are produced, how they are transported and how and where they are perceived, but also to quantitat...
Article
Full-text available
Interactions between physical forces and membrane proteins underpin many forms of environmental sensation and acclimation. Microbes survive osmotic stresses with the help of mechanically gated ion channels and osmolyte transporters. Plant mechanosensitive ion channels have been shown to function in defense signaling. Here, we engineered genetically...
Preprint
Full-text available
Interactions between physical forces and membrane proteins underpin many forms of environmental sensation and acclimation. Microbes survive sudden osmotic stresses with the help of mechanically gated ion channels and osmolyte transporters. Plant mechanosensitive ion channels have been shown to function in defense signaling. Here, we engineered gene...
Preprint
Genetically encoded fluorescent sugar sensors are valuable tools for the discovery of transporters and for quantitative monitoring of sugar steady-state levels in intact tissues. Genetically encoded Förster Resonance Energy Transfer sensors for glucose have been designed and optimized extensively, and a full series of affinity mutants is available...
Article
Genetically encoded biosensors are powerful tools for quantitative visualization of ions and metabolites in vivo. Design and optimization of such biosensors typically require analyses of large numbers of variants. Sensor properties determined in vitro such as substrate specificity, affinity, response range, dynamic range, and signal-to-noise ratio...
Thesis
Single-molecule Förster Resonance Energy Transfer (smFRET) is a peerless tool for studying molecular structures, dynamics, functions and interactions of biological macromolecules in heterogeneous systems. Nowadays, the technique is mainly limited to in vivo expressed proteins. However, its combination with cell-free protein synthesis (CFPS) appears...
Article
Single-molecule FRET (smFRET) is a powerful tool to investigate molecular structures and conformational changes of biological molecules. The technique requires protein samples that are site-specifically equipped with a pair of donor and acceptor fluorophores. Here, we present a detailed protocol for preparing double-labeled proteins for smFRET stud...
Article
Single-molecule FRET (smFRET) is a powerful tool to investigate conformational changes of biological molecules. In general, smFRET studies require protein samples that are site-specifically double-labeled with a pair of donor and acceptor fluorophores. The common approaches to produce such samples cannot be applied when studying the synthesis and f...
Article
Successful colonization and survival in variable environments require a competitive advantage during the initial growth phase after experiencing nutrient changes. Starved yeast cells anticipate exposure to glucose by activating the Hxt5p glucose transporter, which provides an advantage during early phases after glucose resupply. cAMP and glucose FR...

Network

Cited By

Projects

Projects (2)
Project
Sensors, microfluidics for plant-pathogens
Project
Synthesis of site-specifically and selectively double-labeled proteins for more accurate single-molecule FRET studies. The more convenient cell-free approach is an ideal tool for labs focusing on spectroscopic techniques, since cell cultures and safety regulations for recombinant organisms are not necessary.