Maximino Manzanera

Maximino Manzanera
University of Granada | UGR · Department of Microbiology

Ph.D. in Biochemistry and Molecular Biology

About

74
Publications
33,085
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1,715
Citations
Citations since 2016
27 Research Items
921 Citations
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2016201720182019202020212022050100150200
2016201720182019202020212022050100150200
2016201720182019202020212022050100150200

Publications

Publications (74)
Article
Full-text available
Emerging and unregulated contaminants end up in soils via stabilized/composted sewage sludges, paired with possible risks associated with the development of microbial resistance to antimicrobial agents or an imbalance in the microbial communities. An enrichment experiment was performed, fortifying the sewage sludge with carbamazepine, ketoprofen an...
Article
Full-text available
Droughts and high temperatures deeply affect crop production. The use of desiccationtolerant (or xerotolerant) microorganisms able to protect plants from droughts represents a promising alternative. These xerotolerant microorganisms have previously been used to modulate plant responses and improve their tolerance to drought. In addition, these micr...
Article
Full-text available
Salt stress is a serious agricultural problem threatens plant growth and development resulted in productivity loss and global food security concerns. Salt tolerant plant growth promoting actinobacteria, especially deep-sea actinobacteria are an alternative strategy to mitigate deleterious effects of salt stress. In this study, we aimed to investiga...
Article
Agriculture represents an important mechanism in terms of reducing plant, animal, and microbial biodiversity and altering the environment. The pressure to cope with the increasing food demands of the human population has intensified the environmental impact, and alternative ways to produce food are required in order to minimize the decrease in biod...
Article
Full-text available
Soil salinity is an enormous problem affecting global agricultural productivity. Deep-sea actinobacteria are interesting due to their salt tolerance mechanisms. In the present study, we aim to determine the ability of deep-sea Dermacoccus (D. barathri MT2.1T and D. profundi MT2.2T) to promote tomato seedlings under 150 mM NaCl compared with the ter...
Article
Full-text available
Plant growth-promoting rhizobacteria (PGPR) are beneficial soil microorganisms that can stimulate plant growth and increase tolerance to biotic and abiotic stresses. Some PGPR are capable of secreting exopolysaccharides (EPS) to protect themselves and, consequently, their plant hosts against environmental fluctuations and other abiotic stresses suc...
Chapter
El proceso de aprendizaje resulta de una estimulación basada en la generación de emociones, la cual es clave para distinguir entre el fracaso y el éxito académico (1). En un proyecto cooperativo interdisciplinar entre la facultad de Farmacia y Bellas Artes, se llevó a cabo un trabajo de modelización mediante una impresora 3D, logrando que estudiant...
Article
Full-text available
The detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) during wastewater treatment leads to concerns about whether this process may represent a focal point for the transmission of COVID-19. An epidemiological analysis, based on a COVID-19 IgG/IgM Rapid Test Cassette, performed on 134 wastewater workers from 59 wastewater trea...
Article
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Four granular sequencing batch reactors (GSBRs) were inoculated with four denitrifying Pseudomonas strains carrying nosZ to study the process of granule formation, the operational conditions of the bioreactors, and the carbon concentration needed for nitrate removal. The selected Pseudomonas strains were P. stutzeri I1, P. fluorescens 376, P. denit...
Article
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Pandemics deeply affect the health and economy of the world population. A precise determination of affected communities is of great importance to establish containment measures and reduce the economic impact. Here, we propose the development of Units for Vigilance of Emerging Diseases based on the screening of pathogens released to wastewater treat...
Article
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Desiccation-tolerant plants are able to survive for extended periods of time in the absence of water. The molecular understanding of the mechanisms used by these plants to resist droughts can be of great value for improving drought tolerance in crops. This understanding is especially relevant in an environment that tends to increase the number and...
Article
Agriculture represents an important mechanism for the reduction in plant, animal and microbial biodiversity and the alteration of the environment. The high pressure to cope with increasing demands of food for the human population intensifies the environmental impact and requires alternative ways to produce more food and to minimize the decrease in...
Article
The relevance of preserving microorganisms has been well accepted for several decades. Interest is now shifting towards investigating adequate preservation methods to improve microbial survival rates and to preserve new taxa of previously considered unculturable microorganisms. In addition, a growing interest in preserving fragile microbial consort...
Preprint
Desiccation-tolerant plants are able to survive for extended periods of time in the absence of water. The molecular understanding of the mechanisms used by these plants to resist droughts can be of great value for the improvement of tolerance of sensitive plants with agricultural interest. This understanding is especially relevant in an environment...
Article
Full-text available
Pseudomonas aeruginosa is an opportunistic human pathogen causing infections in a variety of plant and animal hosts. The gene mcpB, part of the chemosensory gene cluster II, encodes a soluble chemoreceptor whose function remains unknown. Previous studies show that the cheB2 gene, also located in the chemosensory cluster II, is involved in a specifi...
Article
Halomonas variabilis strain W10, which was isolated from deepsea water samples, grows and produce exopolymeric substances (EPSs) under a wide range of salinity conditions. However, the characteristics of polymers synthesized varied with salt concentrations. In this study, a production index (PI) reference system to characterize the quality of EPSs...
Article
Biostimulation of the activity of degrading autochthonous microbiota constitutes one of the most accepted bioremediation tools for oil-polluted soils. Applications of nutrients such as nitrogen fertilizers, oleophilic fertilizers or surfactants are some of the strategies employed. Soil microcosm experiments were performed in open glass containers t...
Article
Full-text available
A collection of lipase-producing microorganisms was isolated from sludge derived from an urban wastewater treatment plant. The microorganisms with the highest levels of lipase activity were selected in order to use triglycerides present in the sludge effectively and were then transformed with pdc:adhB genes for the production of ethanol. The transg...
Article
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Polycyclic aromatic hydrocarbons (PAHs) are widely distributed in polluted environments and are included in the priority list of toxic compounds. Previous studies have shown that the fungus Penicillium oxalicum, isolated from a hydrocarbon-polluted pond, has a great capability to transform different PAHs in short periods under submerged fermentatio...
Article
Full-text available
Drought tolerance of plants such as tomato or pepper can be improved by their inoculation with rhizobacteria such as Microbacterium sp. 3J1. This interaction depends on the production of trehalose by the microorganisms that in turn modulate the phyto-hormone profile of the plant. In this work we describe the characterization of metabolic changes du...
Article
In this study we provide the first biochemical characterization of a bacterial pyranose 2-oxidase (AsP2Ox) from Arthrobacter siccitolerans. The enzyme catalyzes the oxidation of several aldopyranoses at the C-2 position, coupling it to the reduction of dioxygen to hydrogen peroxide. Pyranose 2-oxidases belong to the glucose-methanol-choline oxidore...
Article
Full-text available
A collection of desiccation-tolerant xeroprotectant-producing microorganisms was screened for their ability to protect plants against drought, and their role as plant growth-promoting rhizobacteria was investigated in two different crops (tomato and pepper). The most commonly described biochemical mechanisms for plant protection against drought by...
Article
Full-text available
The DNA molecule is associated with the role of encoding information required to produce RNA which is translated into proteins needed by the cell. This encoding involves information transmission to offspring or to other organisms by horizontal transfer. However, despite the abundance of this molecule in both the cell and the environment, its physio...
Research
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Plant growth-promoting bacteria (PGPB) colonize plants and enhance their growth by different mechanisms. Some of these microorganisms may represent a potential threat to human, animal or plant health; however, their use might be approved in parts of Europe if they have been recommended as plant growth enhancers. The current regulatory framework has...
Article
Full-text available
Plant growth-promoting bacteria (PGPB) colonize plants and enhance their growth by different mechanisms. Some of these microorganisms may represent a potential threat to human, animal or plant health; however, their use might be approved in parts of Europe if they have been recommended as plant growth enhancers. The current regulatory framework has...
Article
Full-text available
The genome sequence for Microbacterium sp. strain 3J1, a desiccation-tolerant organism isolated from the Nerium oleander rhizosphere, is reported here. The genome is estimated to be approximately 3.5 Mb in size, with an average G+C content of 67.7% and a predicted number of protein-coding sequences of 3,310. Copyright © 2015 Manzanera et al.
Article
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Arthrobacter koreensis 5J12A is a desiccation-tolerant organism isolated from the Nerium oleander rhizosphere. Here, we report its genome sequence, which may shed light on its role in plant growth promotion. This is believed to be the first published genome of a desiccation-tolerant plant growth promoter from the genus Arthrobacter.
Article
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The genome sequence for Rhodococcus sp. 4J2A2, a newly described desiccation-tolerant strain that removes aromatic hydrocarbons, is reported here. The genome is estimated to be around 7.5 Mb in size, with an average G+C content of 60.77% and a predicted number of protein-coding sequences of 6,354.
Article
Full-text available
We report the first genome sequence for Leucobacter sp. 4J7B1, a newly described desiccation-tolerant strain. The complete genome sequence of Leucobacter sp. 4J7B1 has been sequenced and is estimated to be around 3.5 Mb in size, with an average GC content of 62.18%. We predict 2,953 protein-coding sequences.
Article
Full-text available
We report the first genome sequence for Arthrobacter siccitolerans 4J27, a newly described desiccation-tolerant species. The complete genome of A. siccitolerans 4J27 has been sequenced and is estimated to be around 5.3 Mb in size, with an average GC content of 65.13%. We predict 4,480 protein-coding sequences (CDSs).
Article
Full-text available
A novel desiccation-tolerant, xeroprotectant-producing bacterium, designated strain 4J27T, was isolated from a Nerium oleander rhizosphere subjected to seasonal drought in Granada, Spain. Phylogenetic analysis based on 16S rRNA gene sequencing placed the isolate within the genus Arthrobacter, its closest relative being Arthrobacter phenanthrenivora...
Patent
Microorganismo de la especie bacteriana Arthrobacter sp. con número de acceso CECT7626. La presente invención también se refiere al uso de dicho microorganismo o de una población del mismo para aumentar la tolerancia al estrés hídrico de una planta o para aumentar el crecimiento de una planta en condiciones óptimas de contenido hídrico de dicha pla...
Patent
Microorganismo de la especie bacteriana Microbacterium sp. con número de acceso CECT7624. La presente invención también se refiere al uso de dicho microorganismo o de una población del mismo para aumentar la tolerancia al estrés hídrico de una planta o para aumentar el crecimiento de una planta en condiciones óptimas de contenido hídrico de dicha p...
Article
Full-text available
Plant growth-promoting rhizobacteria (PGPR) increase the viability and health of host plants when they colonize roots and engage in associative symbiosis (Bashan et al. 2004). In return, PGPR viability is increased by host plant roots by the provision of nutrients and a more protective environment (Richardson et al. in Plant Soil 321:305-339, 2009)...
Chapter
Full-text available
We thank the Junta de Andalucia (Spain) for funding this study through project reference P08-RNM-04180 and the Spanish Ministry of Science and Technology for funding through project reference CTM2009-09270. M. Manzanera received grants from the Programa Ramon y Cajal, (Ministerio de Educacion y Ciencia MEC, Spain, and ERDF, European Union).
Patent
Microorganismo de la especie bacteriana Arthrobacter sp. con número de acceso CECT7626. La presente invención también se refiere al uso de dicho microorganismo o de una población del mismo para aumentar la tolerancia al estrés hídrico de una planta o para aumentar el crecimiento de una planta en condiciones óptimas de contenido hídrico de dicha pla...
Patent
Microorganismo de la especie bacteriana Microbacterium sp. con número de acceso CECT7624. La presente invención también se refiere al uso de dicho microorganismo o de una población del mismo para aumentar la tolerancia al estrés hídrico de una planta o para aumentar el crecimiento de una planta en condiciones óptimas de contenido hídrico de dicha p...
Article
Microorganisms and plants establishment for the rhyzoremediation of recalcitrant pollutants in arid and semiarid regions, is further impeded by additional physicochemical factors such as low precipitation, high evaporation, extreme temperatures, and high winds. These abiotic factors contribute to the extremely low water content in soil and reduce t...
Article
Desiccation tolerant microorganisms present a remarkable stability in the dry state. This stability is achieved thanks to the production of xeroprotectors that avoid damages in their biomolecules when dried. We have developed a method for the isolation of desiccation tolerant microorganisms. Their xeroprotectors have been extracted, identified and...
Article
Full-text available
A novel biotechnological process has been developed for the isolation of desiccation-tolerant microorganisms and their xeroprotectants, i.e., compatible solutes involved in long-term stability of biomolecules in the dry state. Following exposure of soil samples to chloroform, we isolated a collection of desiccation-tolerant microorganisms. This col...
Conference Paper
Full-text available
In arid and semiarid regions, the establishment of microorganisms and plants for the bioremediation of polycyclic aromatic hydrocarbons (PAHs) is further impeded by a number of physicochemical factors including low precipitation, high evaporation, high winds and extreme temperatures. These factors contribute to the extremely low water content in so...
Conference Paper
Full-text available
Bioremediation is an important technology for the restoration of oil polluted environments by indigenous or selected microorganisms. In general, the rate of biodegradation depends on the number and types of microorganisms, the nature and chemical structure of pollutants to be degraded and the environmental conditions. In this study we have evaluate...
Article
Full-text available
A novel bacterium, strain BM90, previously isolated from Tyrrhenian Sea, was metabolically characterized testing its ability to use 95 different carbon sources by the Biolog system. The bacterium showed a broad capacity to use fatty-, organic- and amino-acids; on the contrary, its ability to use carbohydrates was extremely scarce. Strain BM90 was i...
Article
Phosphatases, glucosidase, protease, esterase and dehydrogenase activities in a MBR (membrane bioreactor) system equipped with ultrafiltration membranes for the treatment of real urban wastewater were measured at different volatile suspended solid (VSS) concentrations, total suspended solid (TSS) concentrations, hydraulic retention times (HRT), tem...
Article
Full-text available
Biodegradation is one of the primary mechanisms for elimination of petroleum and other hydrocarbon pollutants from the environment. It is considered an environmentally acceptable way of eliminating oils and fuel because the majority of hydrocarbons in crude oils and refined products are biodegradable. Petroleum hydrocarbon compounds bind to soil co...
Article
Full-text available
Pseudomonas putida dried in the presence of hydroxyectoine or trehalose can withstand exposure to organic solvents and therefore can be encapsulated inside plastics such as polystyrene. Here we show that P. putida in a plastic-encapsulated dried tablet exhibits remarkable tolerance to chemical stress, comparable to that of spores of Bacillus subtil...
Article
Full-text available
Biodiesel is an alternative energy source and could be a substitute for petroleum-based diesel fuel. To be a viable alternative, a biofuel should provide a net energy gain, have environmental benefits, be economically competitive, and be producible in large quantities without reducing food supplies. Most of the sources, methods and apparatus to pro...
Article
We demonstrate the self-assembly of DNA and DNA binding proteins into two-dimensional networks that are then addressable by sending a second protein to a specific recognition site on the DNA network. These networks cover centimeters in area but can be addressed with nanometer precision. This hierarchical self-assembly of specific DNA protein comple...
Article
Full-text available
We demonstrate the self-assembly of DNA and DNA binding proteins into two-dimensional networks that are then addressable by sending a second protein to a specific recognition site on the DNA network. These networks cover centimeters in area but can be addressed with nanometer precision, This hierarchical self-assembly of specific DNA protein comple...
Article
Full-text available
Escherichia coli and Pseudomonas putida dried in hydroxyectoine or trehalose are shown to be highly resistant to the organic solvents chloroform and acetone, and consequently, they can be encapsulated in a viable form in solid plastic materials. Bacteria are recovered by rehydration after physical disruption of the plastic. P. putida incorporated i...
Article
The tetrahydropyramidine hydroxyectoine acts as an osmolyte in a range of bacterial species, but its use as a desiccation protectant is less well explored. Recently, it was demonstrated that hydroxyectoine provides effective stabilisation of the Gram-negative species Pseudomonas putida, which is only relatively poorly preserved by the better-charac...
Article
Full-text available
The tmoABCDEF genes encode the toluene-4-monooxygenase from Pseudomonas mendocina KR1. Upstream from the tmoA gene an open reading frame, tmoX, encoding a protein 83% identical to TodX (todX being the initial gene in the todXFC1C2BADEGIH operon from Pseudomonas putida DOT-T1E) was found. The tmoX gene is also the initial gene in the tmoXABCDEF gene...
Article
Full-text available
The genes norCBQD that encode the bc-type nitric oxide reductase from Bradyrhizobium japonicum USDA110 have been isolated and characterized. norC and norB encode the cytochrome c-containing subunit II and cytochrome b-containing subunit I of nitric oxide reductase, respectively. norQ encodes a protein with an ATP/GTP-binding motif, and the predicte...
Article
Full-text available
Anhydrobiotic engineering aims to increase the level of desiccation tolerance in sensitive organisms to that observed in true anhydrobiotes. In addition to a suitable extracellular drying excipient, a key factor for anhydrobiotic engineering of gram-negative enterobacteria seems to be the generation of high intracellular concentrations of the nonre...