Maxim NosenkoTrinity College Dublin | TCD · Biochemistry
Maxim Nosenko
Doctor of Philosophy
MSCA Postdoctoral researcher in David Finlay lab, working on immunometabolism
About
25
Publications
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220
Citations
Citations since 2017
Introduction
Additional affiliations
July 2015 - January 2016
July 2015 - present
February 2013 - June 2015
Education
September 2010 - June 2015
Publications
Publications (25)
Itaconate is an immunoregulatory metabolite produced by myeloid cells and plays a key role in the regulation of the immune response. Itaconate, on the one hand, is able to suppress the activity of succinate dehydrogenase (SDH), thereby making a significant contribution to the metabolic reprogramming of the cell. On the other hand, itaconate can reg...
To date, much remains unclear about the pathogenesis of asthma, one of the most common chronic and highly heterogenic diseases of the respiratory system. The lack of specific and highly effective therapy in case of certain asthma subtypes requires the search for new approaches to treatment. One possible approach would be to influence the metabolism...
Asthma is one of the most common chronic diseases. In many cases it is preceded by the development of an immune response to allergens such as animal fur, dust, pollens and etc. In human population this disease is heterogeneous, and no selective drugs are available at the moment for some endotypes of asthma. The role of the adaptive immune system in...
Bioengineered scaffolds are crucial components in artificial tissue construction. In general, these scaffolds provide inert three-dimensional (3D) surfaces supporting cell growth. However, some scaffolds can affect the phenotype of cultured cells, especially, adherent stromal cells, such as fibroblasts. Here we report on unique properties of 3D fib...
Systemic cytokine inhibition may be an effective therapeutic strategy for several autoimmune diseases. However, recent studies suggest that tissue or cell type–specific targeting of certain cytokines, including TNF, may have distinct advantages and show fewer side effects. Here we describe protocols for generating and testing bispecific cytokine in...
Systemic TNF neutralization can be used as a therapy for several autoimmune diseases. To evaluate the effects of cell type‐restricted TNF blockade, we previously generated bispecific antibodies that can limit TNF secretion by myeloid cells (myeloid cell‐specific TNF inhibitors or MYSTIs). In this study several such variable domain (VH) of a camelid...
In spite of successful therapeutic neutralization of proinflammatory cytokines in several autoimmune diseases, such therapy is not entirely free of side effects. The main reason relates to the fact that cytokine signaling may have protective components that need to be spared. Several approaches to achieve a less damaging cytokine inhibition are bei...
Skin wound healing is subject to an intricate regulation, involves many cell populations and molecular mediators, and is one of the key mechanisms that ensures the barrier functions of the skin and the maintenance of body homeostasis. The efficiency of this process is largely determined by the balance of proinflammatory and proregenerative signals,...
Cytokines comprise the molecular language of communication between the cells, which is needed to maintain the homeostatic functions of the body (including the immune system) and mediate various diseases. Many aspects of inflammation, autoimmune diseases and neoplasia are associated with cytokine signaling through specific receptors. The establishme...
Despite decades of research, the goal of achieving scarless wound healing remains elusive. One of the approaches, treatment with polymeric microcarriers, was shown to promote tissue regeneration in various in vitro models of wound healing. The in vivo effects of such an approach are attributed to transferred cells with polymeric microparticles func...
Systemic inflammation can influence energy balance in the organism, necessary for mobilization of resources for immune response. Many metabolic disorders are associated with inflammation, however the molecular mechanisms, linking inflammation and metabolism, are poorly elucidated. One possible mechanism can be related to proinflammatory cytokine in...
Интерлейкин-6 (IL-6) обладает множеством функций в организме, таких как регуляция воспаления и метаболизма, но также может играть патогенную роль в аутоиммунных заболеваниях и образовании опухолей *1+. Для терапии некоторых заболеваний широко применяются нейтрализующие антитела к IL-6 человека (силтуксимаб, сирукумаб, клазакизумаб, олокизумаб) *2+....
Reverse genetics approach, involving genome editing, makes it possible not only to establish the nonredundant and unique functions of genes and their products, but also to construct animal models for biomedical research. Interleukin 6 (IL-6) is an important immunoregulatory and proinflammatory cytokine that differs from many related proteins in hav...
To model human interleukin-6 (hIL-6) associated diseases, unique mice with transgenic overexpression of human IL-6 and reporter fluorescent protein EGFP in cells of macrophage-monocyte lineage were generated using loxP–Cre system. High level of hIL-6 production by macrophages and monocytes, as confirmed in vitro in primary culture of bone marrow-de...
Proinflammatory cytokines, such as TNF, IL-6, and IL-1, play pathogenic roles in multiple diseases and are attractive targets for biologic drugs. Because proinflammatory cytokines possess non-redundant protective and immunoregulatory functions, their systemic neutralization carries the potential for unwanted side effects. Therefore, next-generation...
Culturing of allogeneic or autologous cells in three-dimensional bioresorbable scaffolds is an important step in the engineering of constructs for regenerative medicine, as well as for experimental systems to study the mechanisms of cell differentiation and cell-to-cell interaction. Artificial substrates can modulate the phenotype and functional ac...
The process of tissue regeneration following damage takes place with direct participation of the immune system. The use of biomaterials as scaffolds to facilitate healing of skin wounds is a new and interesting area of regenerative medicine and biomedical research. In many ways, the regenerative potential of biological material is related to its ab...
Myeloid-derived suppressor cells (MDSCs) represent a heterogeneous population of immature myeloid cells (IMCs) that, under normal conditions, may differentiate into mature macrophages, granulocytes, and dendritic cells. However, under pathological conditions associated with inflammation, cancer, or infection, such differentiation is inhibited leadi...
This review addresses the issue of bioengineering of artificial lymphoid organs.Progress in this field may help to better understand the nature of the structure-function relations that exist in immune organs. Artifical lymphoid organs may also be advantageous in the therapy or correction of immunodefficiencies, autoimmune diseases, and cancer. The...
Interleukin-6 (IL-6) is a pleiotropic immunomodulatory cytokine that exhibits crucial functions in the regulation of immune system. However, in some cases, it may induce the development of severe disease manifestations, such as autoimmunity and cancer. Defning cell types that produce "benefcial" and "deleterious" IL-6 in vivo remains one of the mai...
Interleukin-6 (IL-6) is one of the most important proinflammatory cytokines that has a broad spectrum of immunoregulatory properties. The molecular mechanisms of signal transduction of IL-6 and its receptor, which have been previously established, were later supplemented with a concept of trans-signaling. The selective inhibition of this signaling...
Questions
Question (1)
Hey everyone! I have a naive question about SeaHorse analyzer, maybe some of you faced with this problem. In the lab we tried to adapt the SeaHorse mitochondrial stress protocol using bulk metabolic inhibitors from Sigma. At the beginning everything worked totally fine, but later on we found out that our FCCP stock had lost its activity just in a couple of weeks after reconstitution. We tried different approaches like dissolving it in 96% ethanol or DMSO, storing at -80, nothing helps. All the other reagents are stored pretty well. Actually, this is the first time I meet the molecule that is so unstable in standard solvents stored at -80. Does anyone of you dealt with such a problem and found a solution or the only way is to purchase Agilent kits for mitochondria stress test? Weighting microgram amounts for each experiment is also an option, but we don't have the scales with such precision..
