Max-Joseph Kraus

Max-Joseph Kraus
Universität Koblenz-Landau · Institute of Medical Technology and Information Processing

Dr. med. Dr. med (univ. bud.) M. Sc.

About

26
Publications
38,015
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62
Citations
Introduction
I am interested in the reproducible documentation and exact mathematical description of platelet shape change and it's dynamics.
Additional affiliations
February 2005 - present
Praxis Geiselgasteig
Position
  • Medical practioner
August 2002 - January 2003
Universitätsklinikum Erlangen
Position
  • PostDoc Position
January 2000 - September 2001
Universitätsklinikum Carl Gustav Carus Dresden
Position
  • PostDoc Position
Education
September 2010 - January 2012
September 2001 - August 2002
Universität Heidelberg
Field of study
  • Medical Informationmanagement
September 1992 - August 1999
Semmelweis University
Field of study
  • Medicine

Publications

Publications (26)
Article
Many conventional microscopy techniques for investigating platelet morphology such as electron or fluorescence microscopy require highly invasive treatment of the platelets such as fixation, drying and metal coating or staining. Here, we present two unique but entirely different microscopy techniques for direct morphology analysis of live, unstaine...
Article
Full-text available
Blood platelets are highly sensitive circulating subcellular sensors that respond to a multitude of stimuli with a change of their resting discoid shape into more rounded structures processing blebs and pseudopodia. The platelet outline can be automatically segmented from digital images and shows a fractal behaviour. Due to the sensitivity of plate...
Article
Full-text available
Abstract Platelet shape change is a dynamic membrane surface process that exhibits remarkable morphological heterogeneity. Once the outline of an irregular shape is identified and segmented from a digital image, several mathematical descriptors can be applied to numerical characterize the irregularity of the shapes surface. 13072 platelet outlines...
Conference Paper
Full-text available
Text: Aim : Cell surfaces exhibit fractal behaviour. Platelet shape change (PSC) is a dynamic membrane surface process of high morphological irregularity and heterogeneity. We examined, if the fractal Hausdorff dimension (Df) can be used to mathematical describe PSC and compared Df to euclidian geometrical measures of PSC. Method: 5 µL of platelet...
Article
Background: Early diagnosis of Alzheimer's disease (AD) is challenging, and easily accessible biomarkers are an unmet need. Blood platelets frequently serve as peripheral model for studying AD pathogenesis and might represent a reasonable biomarker source. Objective: In the present study, we investigated the potential to differentiate AD patient...
Poster
Full-text available
Background Platelet shape change and spreading is regarded a process particularly sensitive to a multitude of preanalytical factors such as the type of anticoagulant used. We initiated a prospective and blinded study to quantitatively evaluate the impact of different preanalytical conditions on platelet morphology. Method A standardized protocol wa...
Poster
Full-text available
Introduction The current study investigates the possibility to differentiate early and late onset Alzheimers Diseases (AD) patients from each other and from a healthy control population by employing a combined multivariate analysis of platelet shape data, patient blood count as well as aggregation- and flow cytometry markers. Methods A total of 4...
Poster
Full-text available
A plethora of microscopic procedures allows for the qualitative depiction of blood platelet shape change. While each imaging procedure has its specific advantages and disadvantages to visualize specific aspects of platelet shape change, the different results can not be directly quantitatively compared. Based on our previous research on the segmenta...
Article
Full-text available
Blood platelets are highly sensitive circulating subcellular sensors that respond to a multitude of stimuli with a change of their resting discoid shape into more rounded structures processing blebs and pseudopodia. The platelet outline can be automatically segmented from digital images and shows a fractal behaviour. Due to the sensitivity of plate...
Poster
Full-text available
Background The automated morphometry of blood platelets [1] allows for the numerical description of platelet shape and its dynamic change. It is based on the algorithmic analysis of a series of microscopic images. For valid results a standardized microscopic examination and documentation procedure is needed. Therefore an automated microscope system...
Conference Paper
Full-text available
Platelet activation and cell surface dynamics draws significant interest in the scientific community. The current work focuses on the question whether simple geometrical features derived from platelets scanned using standard light microscopy techniques are useful predictors of platelet shape change. The corresponding parameters, such as area or per...
Conference Paper
Full-text available
AIM The geometrical dimensions of single platelets can be automatically determined from microscopic images [1]. We examined the numerical differences in platelet shape after activation with ADP and TRAP. METHOD Platelet rich plasma from anticoagulated whole blood (3.2% buffered sodium-citrate 0,105 M) was prepared and microscopic images of pl...
Conference Paper
Full-text available
AIM The geometrical dimensions of single platelets can be automatically determined from microscopic images [1]. We applied a newly developed algorithm [2] to track individual platelets during microscopic time-series imaging [3]. METHOD 5 µl of platelet rich plasma from anticoagulated whole blood (3.2% buffered sodium-citrate 0,105 M) were prepared...
Conference Paper
Full-text available
AIM The geometrical dimensions of single platelets can be automatically determined from microscopic images [1]. Repeated examinations were performed to assess the test-retest reliability of this automated morphometric approach. METHOD 5 µl of unfixed PRP were prepared and microscopic images of platelets were acquired as described [1]. The focus rem...
Data
Full-text available
Conference Paper
Full-text available
Background Platelet shape is traditionally classified into morphological categories (e.g. discoid, round, dendritic). Fractal Hausdorff-Besicovitch dimension (FD) and circularity (C) are mathematical descriptors for the surface roughness and roundness of irregular shapes. They can be applied to the outlines of platelets automatically detected on mi...
Thesis
Full-text available
Summary Background and objectives Blood platelets respond to many stimuli by changing shape from resting, discoid forms into more rounded structures processing blebs and pseudopodia. Morphological change of platelet structure during activation is generally referred as the platelet shape change (PSC) reaction. PSC is a dynamic process and shows a re...
Article
Full-text available
BACKGROUND: Platelet shape change is a dynamic process that has been classified in different types. Exact documentation of platelet structure needs an improved method of measuring platelet shape. METHODS: 10 μl of platelet-rich plasma (PRP) from anticoagulated whole blood (3.2% buffered sodium citrate 0.105 mol/l) was put onto a glass slide covered...
Conference Paper
Full-text available
Aim Measuring platelet shape change can be improved due to the exact documentation of platelet structure. A new standardized method allows measuring platelet diameter and surface structure to detect the dynamic of platelet shape change in platelet rich plasma (PRP). Methods 20 µL of PRP from anticoagulated whole blood (3.2% buffered Sodium-Citrate...

Questions

Question (1)
Question
I am looking for a fluid solution to close the lateral borders of a coverslip against the diffusion of air under the slip. I used nail polish before. This works fine, but I would like to use something less toxic. It is important, that I must not apply even minimal pressure to the coverslip. Best would be some chemically clearly defined fluid that will not interact with my specimen (living platelets, very sensitive).

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