Mauricio Baptista

Mauricio Baptista
University of São Paulo | USP · Department of Biochemistry (IQ)

PhD
Full professor of Biochemistry, Universidade de São Paulo

About

282
Publications
97,133
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
9,087
Citations
Introduction
Mauricio Baptista currently works at the Department of Biochemistry (IQ), University of São Paulo. Mauricio does research in Chemistry, Biology and Medicine. Their current project deals with the effect of light in biological tissues aiming to develop better sun protecting agents as well as better drugs for Photodynamic Therapy
Additional affiliations
April 2006 - July 2006
University Joseph Fourier - Grenoble 1
Position
  • Professor
March 1998 - present
University of São Paulo
Position
  • Professor (Full)
Description
  • Research professor
January 1997 - January 1998
University of Wisconsin–Madison
Position
  • Research Associate
Description
  • Postdoctoral fellow
Education
July 1992 - September 1996
Marquette University
Field of study
  • Chemistry
February 1991 - July 1992
University of São Paulo
Field of study
  • Biochemistry
March 1986 - November 1990
University of São Paulo
Field of study
  • Pharmacy and Biochemistry

Publications

Publications (282)
Article
Cellular oxidative stress contributes to solar ultraviolet (UV) radiation‐induced skin photoaging and photocarcinogenesis. Light‐driven electron and energy transfer reactions involving non‐DNA chromophores are a major source of reactive oxygen species (ROS) in skin, and the molecular identity of numerous endogenous chromophores acting as UV‐photose...
Article
The unbridled dissemination of multidrug‐resistant pathogens is a major threat to global health and urgently demands novel therapeutic alternatives. Antimicrobial photodynamic therapy (aPDT) has been developed as a promising approach to treat localized infections regardless of drug resistance profile or taxonomy. Even though this technique has been...
Article
Aims Mitochondrial (mt) DNA replication is strongly associated with oxidative stress, a condition triggered by aging and hyperglycemia, both of which contribute to mitophagy disruption and inflammation. This observational exploratory study evaluated mtDNA-copy number (mtDNA-CN) and expression of genes involved in mitochondriogenesis (PPARGC1A, TFAM...
Article
Hydroxypyranoflavylium (HPF) cations are synthetic analogues possessing the same basic chromophore as the pyranoanthocyanins that form during the maturation of red wine. HPF cations absorb strongly in the visible spectral region, and most are fluorescent, triplet‐sensitize singlet oxygen formation in solution and are strong photooxidants, propertie...
Chapter
The development of improved photosensitizers is a key aspect in the establishment of photodynamic therapy (PDT) as a reliable treatment modality. In this chapter, we discuss how molecular design can lead to photosensitizers with higher selectivity and better efficiency, with focus on the importance of specific intracellular targeting in determining...
Article
Lipid peroxidation (LPO) plays a key role in many age-related neurodegenerative conditions and other disorders. Light irradiation can initiate LPO through various mechanisms and is of importance in retinal and dermatological pathologies. The introduction of deuterated polyunsaturated fatty acids (D-PUFA) into membrane lipids is a promising approach...
Article
Full-text available
Over the past two years, during the coronavirus (COVID-19) pandemic, science has been on the hotspot as it became the route to save lives, to avoid individual sickness, to decrease the level of population infection, and to create new solutions to easy the viremia. Light (specially UV radiation, but also visible light) is known to affect virus infec...
Chapter
Photoinduced lipid oxidation starts with light absorption by photosensitizers, forming excited states that oxidize lipids by two different mechanisms. The first mechanism involves energy transfer to molecular oxygen, forming singlet oxygen (Type II). Singlet oxygen reacts with unsaturated lipids, yielding lipid hydroperoxides. Alternatively, excite...
Article
Full-text available
Upon excitation by visible light (VL), melanin engages in photosensitization reactions, generating singlet oxygen (¹O2) and causing oxidative damage to biomolecules via both type I and type II mechanisms. Therefore, VL irradiation may be used to perturb the survival and growth of pigmented melanoma lesions. However, this hypothesis has not been inv...
Article
The formation of hydrogels by photosensitized oxidation and crosslinking of histidine-derived polymers is demonstrated for the first time. The photooxidation of pendant His mediated by singlet oxygen was used to promote covalent coupling by its dimerization. As a proof-of-concept, two systems were studied: (i) chondroitin sulfate (CS) functionalize...
Article
The 20th IUPAB Congress took place online, together with the annual meetings of the Brazilian Biophysical Society and the Brazilian Society for Biochemistry and Molecular Biology, from the 4th to the 8th of October, 2021. The ten keynote lectures, 24 symposia, two poster sessions, and a series of technical seminars covered the full diversity of cur...
Article
Redoxome is the network of redox reactions and Redox Active Species (ReAS) that affect the homeostasis of cells and tissues. Due to the intense and constant interaction with external agents, the human skin has a robust redox signaling framework with specific pathways and magnitudes. The establishment of the skin redoxome concept is key to expanding...
Article
Full-text available
Leishmaniasis is a serious and endemic infectious disease that has been reported in more than 90 countries and territories. The classical treatment presents a series of problems ranging from difficulty in administration, development of resistance, and a series of side effects. Photodynamic therapy (PDT) has already shown great potential for use as...
Article
Retinoblastoma is a malignant tumor of the retinal precursor cells and one of the rarest types of pediatric tumor, often occurring in the earliest years of life. Symptoms are conditioned by tumor size and location; one of the most recurrent symptoms is a white reflex in the pupillary area, called leukocoria or cat's eye reflex. In the present work,...
Preprint
Full-text available
The melanins are a ubiquitous class of pigments found throughout nature from lower organisms to humans. It is one of the few biopolymers with fascinating functions in biology, medicine, chemistry, physics and engineering due to its relation to neurological diseases, skin disorders and melanoma, and, in the last couple of decades, its applications i...
Article
Full-text available
The development of resistance against photodamage triggered by photodynamic therapy (PDT) is ascribed mainly to the cellular redox defenses and repair. If the tumor tissue is not promptly eliminated by the first few PDT sessions, PDT-resistance can be favored, challenging the efficacy of the treatment. Although the mechanism of PDT resistance is st...
Article
Full-text available
Photoexcitation of endogenous photosensitizers (ePS) in the human skin by sunlight culminates with the formation of electronic excited states, such as triplet excited states, singlet oxygen, and a variety of reactive oxygen and nitrogen species, either free radicals or two-electron oxidants. Considering the endogenous skin photosensitizers, melanin...
Article
Photosensitization reactions have been demonstrated to be largely responsible for the deleterious biological effects of UV and visible radiation, as well as for the curative actions of photomedicine. A large number of endogenous and exogenous photosensitizers, biological targets and mechanisms have been reported in the past few decades. Evolving fr...
Article
Full-text available
Conjugation of photosensitizers (PS) with nanoparticles has been largely used as a strategy to stabilize PS in the biological medium resulting in photosensitizing nanoparticles of enhanced photoactivity. Herein, (Meso-5, 10, 15, 20-tetrakis (3-hydroxyphenyl) phorphyryn (mTHPP) was conjugated with diamond nanoparticles (ND) by covalent bond. Nanocon...
Article
Lipid hydroperoxides are the primary reaction products of lipid oxidation, a natural outcome of life under oxygen. While playing a major role in cell metabolism, the microscopic origins of the effects of lipid hydroperoxidation on biomembranes remain elusive. Here we probe the polar structure of partially to fully hydroperoxidized bilayers of 1-pal...
Article
Full-text available
Background Glioblastoma is the most frequent and high-grade adult malignant central nervous system tumor. The prognosis is still poor despite the use of combined therapy involving maximal surgical resection, radiotherapy, and chemotherapy. Metabolic reprogramming currently is recognized as one of the hallmarks of cancer. Glutamine metabolism throug...
Article
Background Bovine digital dermatitis (BDD) is one of the most important diseases that affect dairy cows. Methylene blue-mediated antimicrobial photodynamic therapy (MB-APDT) emerges as a promising technique to treat superficial infections in bovines. Methods Twenty BDD lesions located at the skin horn transition of the claw of pelvic limbs of 16 c...
Article
Full-text available
This Commentary describes a call for submissions for the upcoming Special Issue focused on the science presented at the 20th IUPAB Congress to be held in conjunction with the 45th Annual Meeting of the Brazilian Biophysical Society and the 49th Annual Meeting of the Brazilian Society for Biochemistry and Molecular Biology.
Article
A systematic study was carried out to evaluate the uptake and cytotoxicity of methotrexate (MTX) conjugated to superparamagnetic iron oxide nanoparticles (SPIONs) modified with glycerol phosphate (Glyc) and phosphorylethanolamine (PEA), using MCF-7 cancer cell line as model. The ligand shell composition was controlled in such a way to get SPIONs wi...
Article
Full-text available
In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monit...
Preprint
Full-text available
In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monit...
Article
The consensus on the negative effects of excessive sun exposure on human health has long emphasized the negative effects of solar UV radiation. Nevertheless, although UV radiation has been demonized, less is known about the consequences of sun exposure while using sunscreen, which can lead to high visible light exposure. UV and visible light play a...
Article
Full-text available
Cancer is considered an age-related disease that, over the next 10 years, will become the most prevalent health problem worldwide. Although cancer therapy has remarkably improved in the last few decades, novel treatment concepts are needed to defeat this disease. Photodynamic Therapy (PDT) signalize a pathway to treat and manage several types of ca...
Article
Lipophilic photosensitizers able to photo-induce lipid oxidation in biomembranes are, in general, much more efficient than hydrophilic ones; acting through the formation of singlet oxygen (¹O2), which oxidizes the fatty acid double bonds (type II mechanism). Here we investigate the binding and photosensitizing properties of 4-(decyloxy)pteridin-2-a...
Preprint
Full-text available
the PDF can be download freely on pubmed. https://pubmed.ncbi.nlm.nih.gov/33634751/
Cover Page
Full-text available
In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monit...
Article
Full-text available
In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monit...
Article
Full-text available
Plant extracts (PEs) are frequently used as ingredients in skin and hair care products to improve protection against the excess of sun exposure. PEs are usually selected based on their antioxidant capacity for reducing the damage in lipids and proteins, which are key molecules in the maintenance of skin homeostasis and hair integrity. Interestingly...
Article
Full-text available
Lack of effective treatments for aggressive breast cancer is still a major global health problem. We have previously reported that photodynamic therapy using methylene blue as photosensitizer (MB-PDT) massively kills metastatic human breast cancer, marginally affecting healthy cells. In this study, we aimed to unveil the molecular mechanisms behind...
Article
The lipid composition impacts directly on the structure and function of the cytoplasmic as well as organelle membranes. Depending on the type of membrane, specific lipids are required to accommodate, intercalate, or pack membrane proteins to the proper functioning of the cells/organelles. Rather than being only a physical barrier that separates the...
Article
Introduction The production of β-lactamases by Gram-negative bacteria is among the most important factors of resistance to antibiotics, which has contributed to therapeutic failures that currently threaten human and veterinary medicine worldwide. Antimicrobial photodynamic therapy and antimicrobial blue light have a broad-spectrum antibacterial act...
Article
Abstract The lipid composition impacts directly on the structure and function of the cytoplasmic as well as organelle membranes. Depending on the type of membrane, specific lipids are required to accommodate, intercalate, or pack membrane proteins to the proper functioning of the cells/organelles. Rather than being only a physical barrier that sepa...
Article
Aim: To develop a series of superparamagnetic iron oxide nanoparticles (SPIONs) by coconjugating them with ibuprofen (ibu) and glycerol phosphate (glycerol) or ibu and glucose-1-phosphate and to assess capacity of these conjugates to inhibit the release of nitric oxide (NO) in macrophages, even at low concentrations. Materials & methods: The SPION...
Article
UV‐chromophores contained in human skin may act as endogenous sensitizers of photooxidative stress and can be employed therapeutically for the photodynamic elimination of malignant cells. Here we report that 6‐formylindolo[3,2‐b]carbazole (FICZ), a tryptophan‐derived photoproduct and endogenous aryl hydrocarbon receptor agonist, displays activity a...
Article
A dysfunction in the mitochondrial-lysosomal axis of cellular homeostasis is proposed to cause cells to age quicker and to accumulate lipofuscin. Typical protocols to mediate lipofuscinogenesis are based on the induction of the senescent phenotype either by allowing many consecutive cycles of cell division or by treating cells with physical/chemica...
Article
Full-text available
The global dissemination of the novel coronavirus disease (COVID-19) has accelerated the need for the implementation of effective antimicrobial strategies to target the causative agent SARS-CoV-2. Light-based technologies have a demonstrable broad range of activity over standard chemotherapeutic antimicrobials and conventional disinfectants, neglig...
Preprint
Lack of effective treatments for aggressive breast cancer is still a major global health problem. We previously reported that Photodynamic Therapy using Methylene Blue as photosensitizer (MB-PDT) massively kills metastatic human breast cancer, marginally affecting healthy cells. In this study we aimed to unveil the molecular mechanisms behind MB-PD...
Article
In order to understand the intracellular delivery of drugs, as well as to improve the cell killing efficiency of photosensitizers (PSs) used in Photodynamic Therapy (PDT), we prepared TyroSphere nanoparticles, which are triblock polymer [poly(ethylene glycol)-block-oligo(desaminotyrosyltyrosine octyl ester suberate)-block-poly(ethylene glycol)] agg...
Article
Microbial drug-resistance demands immediate implementation of novel therapeutic strategies. Antimicrobial photodynamic therapy (aPDT) combines the administration of a photosensitizer (PS) compound with low-irradiance light to induce photochemical reactions that yield reactive oxygen species (ROS). Since ROS react with nearly all biomolecules, aPDT...
Article
Full-text available
Background. Diabetes mellitus is a highly prevalent disease with rapid universal growth. In 2013, there were already 382 million people with diabetes, and it is expected that by 2035, this number will double. Chronic hyperglycemia causes a series of biochemical and structural changes, especially in the eyes, kidneys, heart, arteries, and peripheral...
Cover Page
Full-text available
The 3D-printed scaffold can upconvert NIR laser irradiation into visible light. Higher transparency of biological tissues at NIR wavelengths enables delivering visible light to deep-layers to locally activate a photosensitizer drug (erythrosine B), which in turns generates reactive oxygen species (ROS) involved in tumor cell destruction. The scaffo...
Chapter
Recent advances in the research into skin protection from damage caused by the sun, clearly illustrates the need to adjust photoprotection policies. The old idea of sunbathing for short periods of time (without using sunscreen) and avoiding overexposure, even with filter application, seems to be the best option available. Comprehensive photoprotect...
Article
Full-text available
Lipid oxidation encompasses chemical transformations affecting animals and plants in many ways, and light is one of the most common triggers of lipid oxidation in our habitat. Still, the molecular mechanisms and biological consequences of photoinduced lipid oxidation were only recently understood at the molecular level. In this review, we focus on...
Article
The effect of the ligand shell on the cellular uptake efficiency was evaluated by a systematic study using fully dispersed 6 nm diameter superparamagnetic iron oxide nanoparticles (SPIONs), mono and bis-conjugated with glycerol phosphate (glyc), dopamine (dopa), 4,5-dihydroxy-1,3-benzenedisulfonic acid (tiron) and phosphorylethanolamine (pea). Nega...
Article
Full-text available
Photodynamic therapy (PDT) appears as a promising alternative in the treatment of breast cancer since it can be highly effective in curing cancer while preserving normal tissue. However, predicting outcomes in PDT still constitutes a great challenge. One of the parameters that is usually empirically determined is the rate of photon flux delivered t...
Article
Although Photodynamic Therapy (PDT) of cancer has been continuously improved, its efficiency is still limited by the high toxicity in the absence of irradiation, aggregation and deactivation by biomolecules of the most common photosensitizers (PS). The association of PS to nanoparticles (NPs) can be a promising tool to overcome these limitations an...
Article
Food safety and quality management play a pivotal role in the dairy industry. Milk is a highly nutritious food that also provides an excellent medium for growth of pathogenic microorganisms. Thus, dairy industry focuses most of their processes and costs on keeping contamination levels as low as possible. Thermal processes for microbial decontaminat...
Conference Paper
Introduction: The most frequent primary tumor of the central nervous system is the malignant glioma, being the glioblastoma (GBM), grade IV astrocytoma, the most aggressive and lethal glioma. Malignant astrocytomas are responsive for therapy targeting autophagy as temozolomide, the standard adjuvant treatment which induces autophagic cell death. Au...
Article
Full-text available
Upconversion nanoparticles (UCNPs) are suitable materials for bioapplications due to their ability to emit visible light under near-infrared (NIR) excitation, in the biological transparency range. Polycaprolactone(PCL)-based scaffolds are widely used in tissue engineering in combination with inorganic compounds to improve bioactivity and osteocondu...
Article
Full-text available
Photostability is considered a key asset for photosensitizers (PS) used in medical applications as well as for those used in energy conversion devices. In light-mediated medical treatments, which are based on PS-induced harm to diseased tis-sues, the photoinduced cycle of singlet oxygen generation has always been considered to correlate with PS eff...
Article
In this review, we describe how photooxidation changes membrane properties that can ultimately lead to permanent membrane damage. Lipid photooxidation occurs in the presence of reactive oxygen species such as singlet oxygen and by direct reactions of lipids with a photosensitizer in the excited state. Indeed, lipid oxidation triggers chemical trans...
Article
Full-text available
The search for conditions that maximize the outcome of Photodynamic Therapy (PDT) continues. Recent data indicate that PDT-induced cell death depends more on the specific intracellular location of the photosensitizer (PS) than on any other parameter. Indeed, knowledge of the PS intracellular location allows the establishment of clear relationships...
Conference Paper
Full-text available