Mauricio Arenas-Salinas

Mauricio Arenas-Salinas
Universidad de Talca · Center for Bioinformatics and Molecular Simulations (CBSM)

Ph.D Biotechnology

About

40
Publications
5,798
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242
Citations
Citations since 2016
36 Research Items
233 Citations
201620172018201920202021202201020304050
201620172018201920202021202201020304050
201620172018201920202021202201020304050
201620172018201920202021202201020304050

Publications

Publications (40)
Article
Full-text available
Interleukin-1β (IL-1β) is an important cytokine that modulates peripheral and central pain sensitization at the spinal level. Among its effects, it increases spinal cord excitability by reducing inhibitory Glycinergic and GABAergic neurotransmission. In the brain, IL-1β is released by glial cells in regions associated with pain processing during ne...
Article
Full-text available
Shiga toxin-producing Escherichia coli (STEC) cause diarrhea and dysentery, which may progress to hemolytic uremic syndrome (HUS). Vaccination has been proposed as a preventive approach against STEC infection; however, there is no vaccine for humans and those used in animals reduce but do not eliminate the intestinal colonization of STEC. The OmpT,...
Article
Full-text available
Background: In the field of protein engineering and biotechnology, the discovery and characterization of structural patterns is highly relevant as these patterns can give fundamental insights into protein-ligand interaction and protein function. This paper presents GSP4PDB, a bioinformatics web tool that enables the user to visualize, search and e...
Preprint
Shiga toxin-producing Escherichia coli (STEC) cause diarrhea and dysentery, which may progress to hemolytic uremic syndrome (HUS). Vaccination has been proposed as a preventive approach against STEC infection; however, there is no vaccine for humans and those used in animals reduce but do not eliminate the intestinal colonization of STEC. The OmpT,...
Article
The main goal of this work was to evaluate the performance of β-galactosidase from Exiguobacterium acetylicum MF03 in both hydrolysis and transgalactosylation reactions from different substrates. The enzyme gene was expressed in Escherichia coli BL21 (DE3), sequenced, and subjected to bioinformatic and kinetic assessment. Results showed that the en...
Article
Full-text available
The dispersal of mobile genetic elements and their gene cargo relies on type IV secretion systems (T4SS). In this work the ICEAfe1 Tra-type T4SS nanomachine, encoded in the publicly available genome of Acidithiobacillus ferrooxidans ATCC 23270TY, was characterized in terms of its organization, conservation, expression and mating bridge formation. T...
Article
Full-text available
Microbes are suitable candidates to recover and decontaminate different environments from soluble metal ions, either via reduction or precipitation to generate insoluble, non-toxic derivatives. In general, microorganisms reduce toxic metal ions generating nanostructures (NS), which display great applicability in biotechnological processes. Since th...
Data
Phylogenetic trees based on the 16S rRNA gene sequence. Trees were constructed using the neighbor-joining method as described in Methods. (A) Exiguobacterium, (B) Enterobacter, (C) Acinetobacter, and (D) Staphylococcus. The numbers on the nodes represent the percentage of bootstrap (1,000 repetitions).
Data
Growth curves of E. coli and L. monocytogenes exposed to NS. Effect of TeNS synthesized by crude extracts of cells overexpressing the E. cloacae gorA gene in E. coli (A) and L. monocytogenes (B). Effect of TeNS synthesized using crude extracts of MF09 in E. coli (C) and L. monocytogenes (D). Effect of AuNS synthesized by crude extracts of MF09 in E...
Data
Identification, cloning, and purification of GorA. Tellurite (A) and gold (B) reductase activity in situ. Crude extracts from each strain [MF01 (1), MF02 (2), MF03 (3), MF04 (4), MF05 (5), MF06 (6), MF07 (7), MF08 (8), MF09 (9), MF10(10)] were fractionated by NATIVE-PAGE and revealed as TeO32- or AuCl4- activity as described in Methods. (C) SDS-PAG...
Data
in vitro metal(loid)- reduction by crude extracts of the indicated strains. The reduction of TeO32-(A), AuCl4-(B), and Ag+(C) was performed as described in Methods. The activity was measured at different pH values and monitored for 24 h. Strains analyzed: (1) MF01, (2) MF02, (3) MF03, (4) MF04, (5) MF05, (6) MF06, (7) MF07, (8) MF08, (9) MF09, (10)...
Article
Full-text available
This study describes the in-silico rational design, synthesis and evaluation of cross-linked polyvinyl alcohol hydrogels containing γ-cyclodextrin (γ-CDHSAs) as platforms for the sustained release of prednisone (PDN). Through in-silico studies using semi-empirical quantum mechanical calculations, the effectiveness of 20 dicarboxylic acids to genera...
Article
Full-text available
The Psychrobacter genus is a cosmopolitan and diverse group of aerobic, cold-adapted, Gram-negative bacteria exhibiting biotechnological potential for low-temperature applications including bioremediation. Here, we present the draft genome sequence of a bacterium from the Psychrobacter genus isolated from a sediment sample from King George Island,...
Article
Full-text available
This study describes the in-silico design, synthesis, and evaluation of a cross-linked PVA hydrogel (CLPH) for the absorption of organophosphorus pesticide dimethoate from aqueous solutions. The crosslinking effectiveness of 14 dicarboxilic acids was evaluated through in-silico studies using semiempirical quantum mechanical calculations. According...
Article
The electrostatic potential plays a key role in many biological processes like determining the affinity of a ligand to a given protein target, and they are responsible for the catalytic activity of many enzymes. Understanding the effect that amino acid mutations will have on the electrostatic potential of a protein, will allow a thorough understand...
Data
Antibiotic resistance determinants found in the P. putida ATH-43 genome.
Data
ANI and pangenome analysis of P. putida ATH-43. (A) Heatmap representing the comparison of the average nucleotide identity among ten P. putida genomes. (B) Pangenome comparison bar chart showing the number of shared genes clustered in cloud (genes in 0–20% of genomes), shell (genes in 20–90% of genomes), soft core (90–99% of genomes), and core geno...
Data
Metal resistance determinants found in the P. putida ATH-43 genome.
Article
Full-text available
At low concentrations, heavy metals and metalloids are highly toxic for most microorganisms (Lemire et al., 2013). Over evolution, bacteria have developed several molecular mechanisms in order to cope with heavy metal/metalloid toxicity (Lemire et al., 2013; Nies, 2000). Pseudomonas putida belongs to a group of versatile microorganisms capable to t...
Article
Full-text available
The tellurium oxyanion tellurite (TeO32-) is extremely harmful for most organisms. It has been suggested that a potential bacterial tellurite resistance mechanism would consist of an enzymatic, NAD(P)H-dependent, reduction to the less toxic form elemental tellurium (Te0). To date, a number of enzymes such as catalase, type II NADH dehydrogenase and...
Data
SDS-PAGE of purified putative tellurite reductases. (A) Purified E. coli flavoproteins E3 (∼56 kDa), YkgC (∼48 kDa), GorA (∼49 kDa), AhpF (∼56 kDa), TrxB (∼35 kDa), SthA (∼52 kDa), and NorW (∼41 kDa) were visualized by PAGE-SDS. Each lane contained 15 μg of protein. (B) Mercuric reductase (MerA, ∼56 kDa, 10 μg) from the environmental plasmid pTP6....
Data
Amino acid sequence alignment of the indicated E. coli flavoproteins. Secondary structure elements of E3 (PDB_ID: 4JDR), GorA (PDB_ID: 1GER), AhpF (PDB_ID: 1FL2), and TrxB (PDB_ID: 1CL0) are indicated at the top (hairsprings: α-helix; arrows, β-sheets). Cys residues are indicated by black dots.
Data
Transmission electron microscopy of in vivo synthesized electron-dense assemblies. The structures resulting from the in vivo tellurite reduction by E. coli strains overproducing AhpF (A), E3 (B), GorA (C), YkgC (D), TrxB (E), and NorW (F) were visualized by TEM as described in Section “Materials and Methods.”
Article
In all ferritns of known structure the short fifth E α-helix acquire an acute angle to the bundle near the C-terminus tail. However, the crystal structure of the recombinant Chlorobium tepidum ferritin (rCtFtn) shows that the presence of Arg150 in the helix E and the fact that the DE loop is formed by two amino acids produced that the helix E acqui...
Article
Full-text available
With advancements in crystallographic technology and the increasing wealth of information populating structural databases, there is an increasing need for prediction tools based on spatial information that will support the characterization of proteins and protein–ligand interactions. Herein, a new web service is presented termed amino acid frequenc...
Article
The dihydrolipoamide dehydrogenase (LpdA) from the tellurite-resistant bacterium Aeromonas caviae ST reduces tellurite to elemental tellurium. To characterize this NADH-dependent activity, the A. caviae lpdA gene was subjected to site-directed mutagenesis and genes containing C45A, H322Y and E354K substitutions were individually transformed into Es...
Article
The tellurium oxyanion, tellurite, is extremely noxious to most living organisms. Its toxicity has been mainly related to the generation of reactive oxygen species (ROS) as well as to an unbalancing of the thiol:redox buffering system. Nevertheless, a few bacteria are capable of thriving at high tellurite concentrations. One mechanism of resistance...

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