
Maura Téllez-Téllez- Universidad Autónoma del Estado de Morelos
Maura Téllez-Téllez
- Universidad Autónoma del Estado de Morelos
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Publications (52)
Background/Objetive. The chili pepper (Capsicum annuum) is one of the most important agricultural crops in Mexico. Due to this, the aims were to determine the in vitro effect of the spent substrate by the edible fungus P. ostreatus, strain HEMIM-50, (SSPO) against N. aberrans and evaluate the in situ effectiveness of the SSPO against N. aberrans in...
Cordyceps s.l. is a group of morphologically, phylogenetically and ecologically diverse fungal species, parasites of insects and arachnids.
Worldwide more than 1000 species are known and in Mexico 48 have been recorded, most of them from the west, center and south of the country. Particularly in the state of Morelos 13 species have been recorded. T...
Fungi are a source of a variety of secondary metabolites of importance in different areas of biotechnology. Several compounds have been characterized with antioxidant, antimicrobial, and anti-inflammatory activity from fungi of the division of the Ascomycota, among which is the species Daldinia eschscholtzii, an endophyte fungus of pantropical dist...
Fungi are a diverse group, and they are essential for health, the economy, and food. Interest in these organisms has increased because of the importance and effect of their chemical components viz., phenolic compounds, which are considered an alternative source of antioxidants. Antioxidants are compounds that prevent cell damage and can help preven...
El hongo Rhizopus stolonifer causa pudrición de frutas y verduras, por lo que es importante la búsqueda de alternativas de control biológico. El objetivo fue realizar antagonismo y evaluar la actividad antifúngica de H. coffeata contra R. stolonifer. Se evaluó el porcentaje de reducción del crecimiento micelial (PRCM) y el tipo de antagonismo de R....
Anti-inflammatory and radical-scavenging activities of the methanolic extract from the leaves of Galphimia spp. collected in Morelos, Mexico, and isolated galphimidin and galphimidin B, were evaluated. Nor-triterpenes galphimidin and galphimidin B displayed anti-inflammatory activity inhibiting the nitric oxide production by the lipopolysaccharide-...
Mushrooms have been used by humans for centuries as food and medicine because they have been shown to affect certain diseases. Mushrooms for medicinal purposes have been consumed in the form of extracts and/or biomass of the mycelium or fruiting body. The beneficial health effects of mushrooms are due to their content of bioactive compounds (polysa...
PurposeIn the present study, the nematicidal activity of the chemical fractionation of the spent substrate of the edible mushroom Pleurotus ostreatus against eggs and L3 larvae of Haemonchus contortus was evaluated.Methods
The hydroalcoholic extract of the spent substrate was subjected to a bipartition with ethyl acetate giving two fractions: one a...
Mushrooms are available as an alternative source to obtain natural pigments (fruiting body, culture medium and mycelium). Pigments have a wide range of applications in the pharmaceutical, cosmetic, food and textile industries. Mushroom pigments are extracellular metabolites, which can be used in many products. Many fungi that degrade wood produce c...
Background: Edible mushrooms have nutraceutical properties, mainly in humans, animals and plants. Pleurotus species represent an alternative approach to parasite control because they have nematicidal activity against different taxonomic genera of nematodes of importance for livestock.
Objective: To present a general overview of fungi of the genus P...
Edible coatings (EC) are a biodegradable alternative for food preservation. In this work EC based on extracellular compounds of Humphreya coffeata and pectin (PHC) were elaborated and characterized through testing on strawberries. Two PHC types were obtained (PHC1, which settled, and PHC2, which floated during the first separation). The conservatio...
Mushrooms are used directly as foods; many species are widely demanded due to excellent flavor, aroma, and texture; they are also considered as functional foods, because their nutritional properties and bioactive compounds promote health. Also they have a great importance in the ecosystem because they perform various environmental functions, such a...
Objective
In the present study, the in vitro and in vivo effect of the hydroalcoholic extract of Neolentinus ponderosus (EHNP) on L3 larvae of Haemonchus contortus was evaluated.Material and methodsThe N. ponderosus fungus was cultivated in potato dextrose liquid medium for 7 days at 120 rpm and 25 °C. Subsequently, the EHNP was obtained; in vitro...
Biofilm is an interesting niche for fungi and essential for growth, pathogenicity, and resistance that form high challenges both in current trends and in the future in the management of fungal diseases. Fungi cause many problems for different organisms in several fields such as in agriculture, livestock, and public health. Fungicides are widely uti...
Background and Aims:
The production of edible fungi is affected by bacterial, fungal and viral diseases, which very often cause large losses. In the production of mushrooms of the genus Pleurotus, the fungi of Trichoderma spp. represent a serious problem of contamination and although there are some chemical compounds that control the infection, the...
Pleurotus ostreatus produces laccase isoforms depending on the conditions in which it grows, it has been suggested that the presence of inducers, the pH of the culture medium as well as the type and concentration of carbon and nitrogen sources promote the production of these isoforms, however, the effect of temperature of Pleurotus ostreatus growth...
The heterologous expression of the gene LACP83 (encoding a laccase) from Pleurotus ostreatus in Escherichia coli was characterized. The laccase enzyme activity and kinetics of bacterial growth with an inducer (IPTG) and without inducer were determined. The maximum enzymatic activity was observed at 7 h post induction with a value of 3740 ± 342 U/L,...
Abstract Activity and zymogram patterns of laccases produced by Pycnoporus cinnabarinus (HEMIM-79) grown in three systems of submerged fermentation (airlift reactor, stirred tank reactor and Erlenmeyer flasks) and in solid-state fermentation using polyurethane foam as inert support, were studied. A culture medium based in glucose, yeast extract and...
Wild mushrooms are an important cultural patrimony, used since time immemorial as food and medicines according to traditional ecological knowledge. Chemical and biological characteristics of the wild mushrooms are of interest because they are a natural source of great importance for the production of compounds with potential biotechnological applic...
Different species of wild edible mushrooms are known in Mexico, which are harvested for consumption or sale in the weekly markets or street markets. Collectors have important knowledge of these species, then, the aim of this study was to determine which species are sold in the market and street markets of the town of Tlayacapan, Morelos. From June...
This research was conducted to extend the knowledge on the differential regulation of laccase genes in response to dyes. In order to accomplish this, we analyzed both, the expression of five laccase genes by real time RT-qPCR, and also the laccase activity and isoforms patterns during the time-course of a Pleurotus ostreatus submerged fermentation...
Abstract
Fungi of the Pycnoporus are efficient degraders of lignocellulosic materials, so they are
classified as white-rot fungi. A distinctive feature is its color ranging from orange to bright red,
attributed to cinnabarin, cinnabarinic acid and tramesanguin mainly, compounds to which have
been attributed some biological activities. This review u...
Total polyphenols and flavonoids contents, as well as ferric reducing antioxidant power (FRAP), metal ions chelating activity, reducing power assay and scavenging activity of DPPH and ABTS radicals in aqueous and methanolic extracts obtained from mycelium, primordium, and fruiting body of Pleurotus ostreatus in both fresh as dry, were evaluated. Th...
In thepresent study, a protein extracted from the fruiting body of Lentinula edodes showed growth inhibition of Escherichia coli and Staphylococcus aureus. An aqueous extract from the dry fruiting body for purification process was used. This process was carried out in three stages, isoelectric focusing, ion exchange, preparative electrophoresis. Th...
Crude extract samples of Pleurotus pulmonarius and Pycnoporus cinnabarinus were taken during growth in liquid broth in an airlift reactor. Growth was monitored indirectly by sugar consumption and pH profile. During growth Pleurotus pulmonarius consumed glucose more slowly than Pycnoporus cinnabarinus, reaching a final pH of 8.0. In contrast, Pycnop...
México es pionero en América Latina para el cultivo de hongos del género Pleurotus y en la actualidad es el segundo hongo más cultivado en nuestro país. En este trabajo se caracterizó el crecimiento en cajas de Petri de nueve cepas de cinco especies del género Pleurotus (P. ostreatus, P. eryngii, P. pulmonarius, P. citrinopileatus and P. djamor var...
Edible mycorrhizal mushrooms have been collected and consumed for centuries in indigenous communities and are part of the diet of its inhabitants. They have economic importance, as several communities supplement their income with the economic trade of these species in specific, in the state of Morelos in the community of Huitzilac these mushrooms a...
The genus Pycnoporus includes saprophytes and basidiomycetes fungi common in tropical and subtropical areas of the world. These fungi have a great potential in the production of metabolites. In this study, two strains of Pycnoporus spp HEMIM-55 and 80 were investigated. The effect of pH culture media (4.5, 5.5, 6.5, 7.5 and 8.5) on radial growth ra...
The genus Pycnoporus includes saprophytes and basidiomycetes fungi common in tropical and subtropical areas of the world. These fungi have a great potential in the production of metabolites. In this study, two strains of Pycnoporus spp HEMIM-55 and 80 were investigated. The effect of pH culture media (4.5, 5.5, 6.5, 7.5 and 8.5) on radial growth ra...
The genus Pycnoporus includes saprophytes and basidiomycetes fungi common in tropical and subtropical areas of the world. These fungi have a great potential in the production of metabolites. In this study, two strains of Pycnoporus spp HEMIM-55 and 80 were investigated. The effect of pH culture media (4.5, 5.5, 6.5, 7.5 and 8.5) on radial growth ra...
The genus Pycnoporus includes saprophytes and basidiomycetes fungi common in tropical and subtropical areas of the world. These fungi have a great potential in the production of metabolites. In this study, two strains of Pycnoporus spp HEMIM-55 and 80 were investigated. The effect of pH culture media (4.5, 5.5, 6.5, 7.5 and 8.5) on radial growth ra...
The genus Pycnoporus includes saprophytes and basidiomycetes fungi common in tropical and subtropical areas of the world. These fungi have a great potential in the production of metabolites. In this study, two strains of Pycnoporus spp HEMIM-55 and 80 were investigated. The effect of pH culture media (4.5, 5.5, 6.5, 7.5 and 8.5) on radial growth ra...
Background: Enzymatic activity and laccase isoenzymes number of Pleurotus ostreatus grown in different pH values of the growing medium in submerged fermentation and incubated in buffer solutions of different initial pH values were determined. The expression profiles of five laccase genes (Lacc1, Lacc4, Lacc6, Lacc9 and Lacc10) in these cultures wer...
The degradation of 2,6-dimethoxyphenol (DMP) and decolorization of Remazol brilliant blue R dye (RBB), added to culture media of Pleurotus ostreatus developed in submerged fermentation, and the laccase, manganese peroxidase and veratryl alcohol oxidase activities produced in these systems were evaluated. Both compounds were removed from the culture...
Laccases (EC 1.10.3.2) are glycoproteins classified as multi-copper oxidases, which catalyze one-electron oxidation of a wide range of inorganic and organic substances, coupled with reduction of oxygen to water.These enzymes are widely distributed in fungi, higher plants, bacteria and insects, however, recently evidence for distribution of laccases...
Zymogram patterns of extracellular laccases of ten strains of Pleurotus grown on agar without addition of inducers, using 2.6-dimethoxyphenol, ρ-anisidine and o-tolidine as substrates, were obtained. Zymogram patterns were only similar for strains within same species, independently of the substrate used, six strains op P. octreatus and one strain o...
In this work, a gene (lacP83) encoding a Pleurotus ostreatus laccase isoenzyme expressed in submerged fermentation conditions is described. A 2,887 bp sequence was obtained from a genomic library of P. ostreatus by using a PCR inverse strategy. The coding sequence, 1,527 bp long, showed 17 exons and encoded a protein of 509 amino acids, with a puta...
Zymogram patterns of extracellular laccases of ten strains of Pleurotus grown on agar without addition of inducers, using 2.6-dimethoxyphenol, ρ-anisidine and o-tolidine as substrates, were obtained. Zymogram patterns were only similar for strains within same species, independently of the substrate used, six strains of P. ostreatus and one strain o...
Pleurotus ostreatus showed atypical laccase production in submerged vs. solid-state fermentation. Cultures grown in submerged fermentation produced laccase at 13,000 U l(-1), with a biomass production of 5.6 g l(-1) and four laccase isoforms. However, cultures grown in solid-state fermentation had a much lower laccase activity of 2,430 U l(-1), bio...
Radial growth rate, intracellular laccases and proteases activities, and protein content were evaluated in five strains of Pleurotus ostreatus, grown on starch-based and glucose-based agar media containing different concentrations of the glucose analogue 2-deoxyglucose (2-DG). Productivity of the strains in pilot scale cultivation was also determin...
The constitutive intracellular laccase activity of ten strains of Pleurotus spp. was determined in vitro and by zymograms, using different substrates. Differences in the in vitro activities were observed between all the strains; however, zymogram patterns were only similar for strains within same species, independently of any of the three substrate...
The intracellular and constitutive laccase activities of the peripheral and central zones of colonies of four strains of Pleurotus, grown on potato dextrose agar were evaluated. In all the strains, hyphae from the periphery (i.e. young hyphae) and from the centre (i.e. mature hyphae) of the vegetative mycelium of the colony showed differences in th...
Aims: To know the ultrastructural and biochemical differences of vegetative hyphae and fruit body initials in colonies of Pleurotus pulmonarius.
Methods and Results: Feulgen reagent was used to detects differentiation of hyphae. The intracellular laccases, proteases and β-1,3-glucanases activity, content of cytoplasmic protein, glycogen and glucans...
The physiology of a colony of Pleurotus pulmonarius grown on potato dextrose agar overlaid with a Cellophane membrane (PDA-WC) was studied. On PDA-WC, the colony presented higher biomass density and productivity of fruit body formation (4.84+/-0.23 mg mycelial biomass/cm2 and 23.7+/-3.12 microg ml(-1) h(-1), respectively) than that which developed...