Maud de Lagarde

• Doctor of Veterinary Medicine
• Adjoint professor at Université de Montréal

Antimicrobial resistance can be effectively limited by improving the judicious use of antimicrobials in food production. However, its effect on the spread of AMR genes in animal populations is not well described. In the province of Québec, Canada, a new legislation implemented in 2019 has led to an unprecedented reduction in the use of critical ant...

Simple Summary There is little information about antimicrobial resistance and the transmission of this resistance in dairy cattle. The aim of this work is to use cutting-edge technology (whole genome sequencing) to characterize antimicrobial resistance of bacteria (Escherichia coli) retrieved from the manure of dairy cattle and to determine how thi...

To tackle antimicrobial resistance (AMR), one of the major health threats of this century, the World Health Organization (WHO) endorsed a global action plan in 2015. This plan calls countries to develop national actions to address AMR. The province of Québec, Canada, adopted a new regulation on the 25th of February 2019, to limit the use in food an...

The definition of a high risk clone for antibiotic resistance dissemination was initially established for human medicine. We propose a revised definition of a high risk clone adapted to the One Health context. Then, we applied our criteria to a cluster of enrofloxacin non susceptible ETEC:F4 isolates which emerged in 2013 in diseased pigs in Quebec...

Aims: Describe the temporal trends in E. coli pathotypes and antimicrobial resistance detected in isolates from diseased-pig cases submitted to the EcL from 2008 to 2016, in Quebec, Canada, and investigate the presence of spatio-temporal and phylogenetic clusters. Methods and results: Detection of 12 genes coding for virulence factors in pathoge...

Simple Summary Antimicrobial resistance has been recognised as a global threat by the WHO. ESBL/AmpC genes, responsible for cephalosporin resistance, are particularly worrisome. Escherichia coli is a ubiquitous bacterium. Most strains are commensal, although some can cause disease in humans and animals. Due to its genome plasticity, it is a perfect...

The objective was to identify the genetic determinants and supports of expanded-spectrum cephalosporin (ESC) resistance in commensal Escherichia coli from healthy horses in France in 2015. Faecal samples from 744 adult horses were screened for ESC-resistant E. coli isolates. The ESBL/AmpC resistance genes were identified using PCR and sequencing. E...

This study evaluated virulence and resistance profiles of Escherichia coli in chicken carcasses from three retail systems in Vietnam. Fresh chicken carcasses from traditional markets and fresh and frozen chicken carcasses from supermarkets were sampled in Vietnam. E. coli isolates from carcass rinses were characterized for extraintestinal pathogeni...

Background Although antimicrobial resistance is increasingly common in equine medicine, molecular and epidemiological data remains scarce. Objectives We estimated the prevalence of, and risk factors for, shedding of multidrug resistant (MDR), extended spectrum β‐lactamase (ESBL)‐producing, and AmpC β‐lactamase‐producing, or some combination of the...

The identification of serum biomarkers of lung inflammation would facilitate the diagnosis of inflammatory airway disease (IAD) in horses. Horses with IAD have higher serum concentrations of markers of inflammation compared to controls. Twelve horses with IAD and 10 control horses. This was a prospective case-control study. Blood and BALF were coll...

Bronchospasm results in airway obstruction in horses with heaves. Atropine is the most potent bronchodilator drug currently available in horses, but is associated with side effects limiting its use. Like atropine, N-Butylscopolamonium bromide (NBB) is an anticholinergic agent with bronchodilatory properties. To compare the bronchodilating and side-...