Matthias Kohl

Furtwangen University | HFU · Faculty of Medical and Life Sciences

The aim of the paper is to give a coherent account of the robustness approach based on shrinking neighborhoods in the case of i.i.d. observations, and add some theoretical complements. An important aspect of the approach is that it does not require any particular model structure but covers arbitrary parametric models if only smoothly parametrized....

Measuring gene transcription using real-time reverse transcription polymerase chain reaction (RT-qPCR) technology is a mainstay of molecular biology. Technologies now exist to measure the abundance of many transcripts in parallel. The selection of the optimal reference gene for the normalisation of this data is a recurring problem, and several algo...

Point-of-care (POC) diagnostics, in particular lateral flow assays (LFA), represent a great opportunity for rapid, precise, low-cost and accessible diagnosis of disease. Especially with the ongoing coronavirus disease 2019 (COVID-19) pandemic, rapid point-of-care tests are becoming everyday tools for identification and prevention. Using smartphones...

Facioscapulohumeral muscular dystrophy (FSHD) is one of the most common autosomal dominant muscle disorders, yet no cure or amelioration exists. The clinical presentation is diverse, making it difficult to identify the actual driving pathomechanism among many downstream events. To unravel this complexity, we performed a meta-analysis of 13 original...

In vorangegangenen Tutorien haben wir uns u.a. mit Konfidenzintervallen [1] und verschiedenen statistischen Tests [2-6] befasst. Diese Verfahren kommen aber nicht nur zum Einsatz, nachdem entsprechende Daten erhoben wurden, sondern spielen von Anfang an eine sehr wichtige Rolle. Im Rahmen der Planung der Studie müssen nämlich insbesondere Überlegun...

In the present work, we aimed to determine the effect of whole-body electromyostimulation (WB-EMS) on metabolic syndrome (MetS) as a cluster of cardiometabolic risk factors in people at moderate-to-high cardiometabolic risk. The present meta-analysis is based on a systematic literature search of a recent evidence map, which searched five electronic...

A Commentary on Commentary: Revised contraindications for the use of non-medical WB-electromyostimulation. Evidence-based German consensus recommendations By Reljic D, Herrmann HJ and Zopf Y (2024). Front Sports Act Living. 6:1425233. doi: 10.3389/fspor.2024.1425233

zu chronischen Schmerzen und Einschränkungen. Patient*innen klagen u. a. über Schmerzen und Steifigkeit in der knieumgebenden Muskulatur und erwarten physiotherapeutische Interventionen zur Linderung dieser Symptome. Es ist unklar, ob die Wahrnehmung von Muskelsteifigkeit mit objektiv erhöhter Muskelsteifigkeit einhergeht. Für die Wahl gezielter Be...

In a randomized, controlled study, whole-body electromyostimulation (WB-EMS) was investigated as a promising alternative treatment technique compared to conventional strength training for the management of knee osteoarthritis (OA). Seventy-two overweight participants with symptomatic knee OA were randomly assigned to WB-EMS (n = 36) or a usual care...

Das wichtigste Ziel in der Medizin ist das Überleben der Patient:innen. Gerade bei schwerwiegenden Erkrankungen wie etwa Krebs geht es darum, mit einer Behandlung ein möglichst langes Überleben der Patient:innen zu erreichen. Entsprechend werden Behandlungen meist dahingehend miteinander verglichen, wie lange die Patient:innen nach der Behandlung w...

Nanopore sequencing has shown the potential to democratize genomic pathogen surveillance due to its ease of use and low entry cost. However, recent genotyping studies showed discrepant results compared to gold-standard short-read sequencing. Furthermore, although essential for widespread application, the reproducibility of nanopore-only genotyping...

OBJECTIVES Adequate theoretical and practical training of prospective clinical perfusionists is essential for maintaining clinical standards and ensuring patient safety during cardiac surgery procedures. Perfusion schools play a crucial role in establishing and maintaining higher education and training standards in clinical perfusion. The aim of th...

Background Although patients with shoulder complaints are frequently referred to physiotherapy, putative predictive factors for outcomes are still unclear. In this regard, only a limited amount of scientific data for patients with subacromial pain syndrome exist, with inconsistent results. An improved knowledge about the ability of baseline variabl...

Introduction: Due to its time-effective, joint friendly and highly customizable character whole-body-electromyostimulation (WB-EMS) is regarded as a feasible solution for a large variety of training issues. Not least for this reason WB-EMS research has increased considerably during the last few years. To identify gaps in research and summarize prev...

Comment on: Cytokines (IL1β, IL6, TNFα) and serum cortisol levels may not constitute reliable biomarkers to identify individuals with post-acute sequelae of COVID-19

Nachdem wir in den letzten Tutorials statistische Signifikanztests [1] für quantitative und ordinale Merkmale [2,3] behandelt haben, werden wir in diesem Tutorial auf wichtige Tests für nominale Merkmale eingehen, wobei wir den k-Stichprobenfall (k ≥ 1) betrachten. Hierbei unterscheiden wir zwischen Merkmalen mit zwei (binär/dichotom) und mehr Merk...

The present systematic review determined the effects of physical activity/exercise added to Denosumab, Romosozumab, Abaloparatide or Teriparatide-therapy on bone strength and fall incidence in middle-aged and older people with osteopenia/osteoporosis. A systematic literature search of five electronic databases and two registers (up to 30/05/2023) w...

Evidence of an anti-inflammatory effect of WB-EMS is growing. However, applying comparable impulse-protocols, studies reporting positive effects on inflammatory markers, possibly including adipokines, used high volume, superimposed WB-EMS protocols that differ from the time-efficient, non-superimposed concepts mainly used in scientific and commerci...

Whole-body electromyostimulation has proven to be a highly effective alternative to conventional resistance-type exercise training. However, due to adverse effects in the past, very extensive contraindications have been put in place for the commercial, non-medical WB-EMS market. Considering recent positive innovations e.g., federal regulation, mand...

In a randomized, controlled study, whole-body electromyostimulation (WB-EMS) was investigated as a promising alternative to conventional strength training for the treatment of knee osteoarthritis (OA). 72 overweight participants with symptomatic knee OA were randomly assigned to WB-EMS (n = 36) or a usual care control group (CG, n = 36). For seven...

Background: Post-pandemic considerations, as well as lessons learned from terrorist attacks, wars, and disasters worldwide demonstrate that emergency departments must be prepared for mass casualty events related to chemical, biological, radiological, and nuclear (CBRN) hazardous materials. Our aim was to evaluate whether German emergency department...

A wide range of approaches can be used to detect micro RNA (miRNA)–target gene pairs (mTPs) from expression data, differing in the ways the gene and miRNA expression profiles are calculated, combined and correlated. However, there is no clear consensus on which is the best approach across all datasets. Here, we have implemented multiple strategies...

Hypertension is a frequent condition in untrained middle-aged to older adults, who form the core group of whole-body electromyostimulation (WB-EMS) applicants. So far, the acute effects of varying impulse intensities on blood pressure responses have not been evaluated in normo- and hypertensive people. Thirteen hypertensive and twelve normotensive...

Background Osteosarcopenia is a common geriatric syndrome with an increasing prevalence with age, leading to secondary diseases and complex consequences such as falls and fractures, as well as higher mortality and frailty rates. There is a great need for prevention and treatment strategies. Methods In this analysis, we used magnetic resonance imag...

Whole-body electromyostimulation (WB-EMS) can be considered as a time-efficient, joint-friendly, and highly customizable training technology that attracts a wide range of users. The present evidence map aims to provide an overview of different non-athletic cohorts addressed in WB-EMS research. Based on a comprehensive systematic search according to...

In den vorangegangenen beiden Tutorials wurde detailliert der Vergleich von Mittelwerten im 1- und 2-Stichprobenfall besprochen [1,2]. In diesem Tutorial wird nun die Situation von drei oder mehr unabhängigen oder abhängigen Gruppen behandelt. Man spricht in diesem Fall auch von einer 1-Weg ANOVA (ANalysis Of VAriance) oder 1-Weg Varianzanalyse. Im...

Physical exercise favorably affects visceral adipose tissue (VAT) which is a risk factor for cardiometabolic diseases. However, many people are unable or unwilling to conduct frequent and intensive exercise programs that have favorable effects on VAT. The present study aimed to determine the effect of the time-efficient and joint-friendly whole-bod...

Background The established therapy of asthma might be supported by additional non-pharmaceutical measures, such as the Buteyko breathing technique (BBT); however, the available data are mixed. To clarify the effects of BBT in patients with asthma, we investigated whether it led to clinical improvements with correlation to functional parameters. Me...

Whole-body electromyostimulation (WB-EMS) can be considered as a time-efficient, joint-friendly and highly customizable training technology that attracts a wide range of users. The present evidence map aimed to provide an overview of different non-athletic cohorts addressed by WB-EMS research. Based on a comprehensive systematic search according to...

Big Data has become part of our everyday lives. Whether you’re using a smartphone, a fitness tracking device, or social media, almost everything in our life generates data points that are being stored, analyzed, and evaluated often without us even thinking about it. The amount of data produced and stored in the Global Datasphere grows vastly every...

Die Analyse und der Vergleich von Mittelwerten gehören zu den am häufigsten durchgeführten statistischen Analysen. Allgemeiner geht es darum, die Lage (Lokation) der Werte von einer, zwei oder mehr Populationen zu bestimmen und mit einem Referenzwert bzw. untereinander zu vergleichen. In den meisten Fällen kommen hierfür statistische Signifikanztes...

Background: Antibiotic use in neonatal intensive care units (NICUs) remains high. Low antibiotic prescribing has been documented among physicians trained in complementary medicine. This study sought to identify if an NICU integrating complementary medicine has low antibiotic prescribing. Methods: We conducted a retrospective analysis at the level-2...

Statistische Tests gehören zu den am häufigsten verwendeten statistischen Verfahren. Sie spielen eine zentrale Rolle, um (Forschungs-)Hypothesen zu prüfen bzw. zu falsifizieren, weshalb man auch von Hypothesentests spricht. Die heute üblicherweise verwendeten statistischen Tests basieren auf der Theorie von Neyman und Pearson (1933) [1] und werden...

Zusammenfassung Stürze sind der wichtigste Risikofaktor für Frakturen im Alter. Epidemiologische Studien haben viele Risikofaktoren für Stürze identifiziert, die durch strukturiertes körperliches Training beeinflussbar sind. Dieser Artikel beschreibt die Evidenz zu Inhalten, Methoden und Belastungsdosierung eines körperlichen Trainings zur Vermeidu...

Early treatment is the mainstay of sepsis therapy. We suspected that early recognition of sepsis by prehospital healthcare providers may shorten the time for antibiotic administration in the emergency department. We retrospectively evaluated all patients above 18 years of age who were diagnosed with sepsis or severe infection in our emergency depar...

Early treatment is the mainstay of sepsis therapy. We suspected that early recognition of sepsis by prehospital healthcare providers may shorten time to antibiotic administration in the Emergency Department. We retrospectively evaluated all patients above 18 years of age that were diagnosed with sepsis or severe infection in our Emergency Departmen...

Exercise is a recognized component in the prevention and therapy of osteoporosis. The present systematic review and meta-analysis aimed to determine the effect of Vitamin D (Vit-D) added to exercise versus exercise alone on bone mineral density (BMD) at the lumbar spine (LS) or hip in older adults. A systematic review based on six literature databa...

We read with interest the article "Myalgic encephalomyelitis/chronic fatigue syndrome: a review of the current evidence" [1] had read. However, literature search and analysis are methodologically inadequate and the results are at odds with the current evidence.

Zusammenfassung Die Definition von relevanten Trainingszielen und deren weitgehend individualisierte Allokation zu Gruppen unterschiedlicher Anforderungen sollte der erste Schritt einer jeden Trainingsstrategie zur optimierten Trainingsplanung und sicheren Trainingsdurchführung sein. Die Festlegung der Trainingsziele im Bereich der Frakturprophylax...

Zusammenfassung Trainingsprotokolle zur Frakturprophylaxe müssen eine Vielzahl von trainingswissenschaftlichen, logistischen und finanziellen Rahmenbedingungen berücksichtigen, um die erwünschte Effektivität und Anwendbarkeit im Einzel- oder Gruppentraining zu gewährleisten. Basierend auf dem individuellen Risikoprofil ist eine Zuordnung von dedizi...

Zusammenfassung Trainingsprinzipien werden als konkrete und leitende Handlungsanweisung für ein körperliches Training angesehen und beziehen sich auf alle übergeordeten Aspekte des Trainings weitestgehend unabhängig von der fokussierten Zielgröße. Relevanz für Gresundheits- und Rehabilitationssport haben primär Prinzipien zur Auslösung und Sicherun...

Zusammenfassung „Trainingsinhalte“ sind „konkrete Maßnahmen zur Realisierung des/der geplanten Trainingsziel(e)s“. Im Bereich der Forschung am Knochen bietet sich eine Einteilung der Trainingsinhalte (oder Belastungstypen) in mechanisch lokale wie axiale Belastung, Muskelzugsbelastung, Belastungsverteilung sowie einen systemisch übergreifenden „Kno...

In der Biomedizin werden heute laufend neue Technologien eingeführt, insbesondere zur quantitativen Messung verschieden­ster Parameter. Diese neuen Messmethoden müssen mit bestehenden (Goldstandard-)Verfahren verglichen werden, um ihre Einsetzbarkeit in der Praxis zu prüfen. Darüber hinaus sind viele bereits etablierte Messmethoden sehr sensitiv ge...

The aim of the present systematic review and meta-analysis was to determine the effects of exercise on low-trauma overall and major osteoporotic fractures in adults. Our systematic search of six literature databases according to the PRISMA guideline was conducted up to May 22, 2021, and included controlled clinical exercise trials with (a) individu...

Zusammenfassung Die Belastungskomponenten bestimmen die Ausrichtung der Trainingsreize und sind somit maßgebend für die Wirkung eines Trainingsprotokolls auf die Knochenfestigkeit. In Anlehnung an die klassische Trainingswissenschaft ist eine Klassifizierung der Belastungskomponenten in Reizhöhe („strain-magnitude“), Reizrate („strain-rate“), Wiede...

Next-generation whole-genome sequencing is essential for high-resolution surveillance of bacterial pathogens, for example, during outbreak investigations or for source tracking and escape variant analysis. However, current global sequencing and bioinformatic bottlenecks and a long time to result with standard technologies demand new approaches. In...

Introduction: Aquatic or water-based exercise is a very popular type of exercise in particular for people with physical limitations, joint problems and fear of falling. The present systematic review and meta-analysis aimed to provide evidence for the effect of aquatic exercise on Bone Mineral Density (BMD) in adults. Methods: A systematic literatur...

In recent decades, nanopores have become a promising diagnostic tool. Protein and solid-state nanopores are increasingly used for both RNA/DNA sequencing and small molecule detection. The latter is of great importance because small molecules are difficult or expensive to detect using available methods such as HPLC or LC-MS. Moreover, DNA aptamers a...

Neben quantitativen und ordinalen Merkmalen, spielen auch nominale Merkmale und deren Zusammenhänge eine wichtige Rolle in der Medizin. Beispiele sind etwa das Geschlecht, Grunderkrankungen, verabreichte Medikamente, durchgeführte Operationen oder auch das Outcome der Patienten. Die Stärke des Zusammenhangs zweier nominaler Merkmale lässt sich jedo...

The aim of this systematic review and meta-analysis was (1) to determine exercise effects on bone mineral density (BMD) in postmenopausal women and (2) to address the corresponding implication of bone and menopausal status or supervision in postmenopausal women. A comprehensive search of eight electronic databases according to the PRISMA statement...

Objectives Due to their pronounced anti-inflammatory and immunosuppressive effects, glucocorticoids (GCs) are widely used in inflammatory conditions and organ transplants. Unfortunately, GC-induced osteoporosis is one of the most common causes of secondary osteoporosis. The aim of the present systematic review and meta-analysis was to determine the...

Fragestellung: Die frühzeitige Einleitung der Therapie ist die tragende Säule in der Behandlung der Sepsis [1]. Wir untersuchten, ob die prähospitale Erkennung einer Sepsis zur Verkürzung der Zeit bis zur Einleitung der Antibiose in der Notaufnahme führt. Methodik: Wir analysierten retrospektiv 277 Patienten, bei denen zwischen 2018 und 2020 in...

Purpose: To evaluate the effect of a high-intensity resistance training (HIT-RT) on visceral adipose tissue (VAT) and abdominal aortic calcifications (AAC). Patients and methods: We conducted a post hoc analysis of the Franconian Osteopenia and Sarcopenia Trial (FrOST). 43 community-dwelling men with osteosarcopenia aged 72 years and older were...

Regular exercise might reduce postmenopausal symptoms, however even short-moderate periods of absence from exercise training might significantly reduce these positive effects. The aim of the study was thus to determine detraining effects on postmenopausal symptoms after a 3-month detraining period in early post-menopausal women. After 13 months, th...

Everyone has or will have experienced some degree of neck pain. Typically, neck pain is associated with the sensation of tense, tight, or stiff neck muscles. However, it is unclear whether the neck muscles are objectively stiffer with neck pain. This study used 1099 ultrasound elastography images (elastograms) obtained from 38 adult women, 20 with...

Vertebral fractures are frequent clinical consequences of osteoporosis. Considering the demographic change in Europe, the number of vertebral fractures will quite likely increase during the next decades. Apart from pharmaceutic agents and physiotherapy, spinal orthoses are established elements of conservative therapy for vertebral (body) fractures....

We read the study by Fleischer et al. [1] with interest. The authors conclude that post-COVID-19 syndrome, also known as long Covid, might be psychosomatic without providing any evidence supporting this conclusion. Their line of logic seems to be that if neurological tests are normal then the condition under study is psychosomatic. However, first o...

Background Hearts procured from circulatory death donors (DCD) are predominantly maintained by machine perfusion (MP) with normothermic donor blood. Currently, DCD heart function is evaluated by lactate and visual inspection. We have shown that MP with the cardioplegic, crystalloid Custodiol‐N solution is superior to blood perfusion to maintain por...

In der modernen Medizin ist man fortwährend auf der Suche nach möglichen Biomarkern, mit denen man etwa Erkrankungen diagnostizieren, den Therapieverlauf verfolgen oder eine Prognose über den Krankheitsverlauf stellen kann. Statistisch gesprochen geht es darum, klinisch relevante Zusammenhänge (Assoziationen) zwischen erhobenen Messgrößen (Variable...

The role of exercise in preventing osteoporotic fractures is vague, and further recommendations for optimized exercise protocols are very rare. In the present work, we provided positive evidence for exercise effects on the number of osteoporotic fractures in adults, albeit without observing any significant relevance of intensity progression or stud...

Inhalt: 1) Die Statistiksoftware R 2) Deskriptive Statistik 3) Farben und Diagramme 4) Wahrscheinlichkeitsverteilungen 5) Schätzen 6) Statistische Hypothesentests 7) Multiples Testen ### Buch und enthaltener R Code: https://github.com/stamats/ESDR

Content: 1) Statistical Software R 2) Descriptive Statistics 3) Colors and Diagrams 4) Probability Distributions 5) Estimation 6) Statistical Tests 7) Multiple Testing ### Book and included R code: https://github.com/stamats/ISDR

Evidence regarding perfusion conditions during extracorporeal cardiopulmonary resuscitation (ECPR) is rare. Therefore, we investigated the impact of perfusion parameters on neurologic outcome and survival in patients with in- or out-of-hospital cardiac arrest (IHCA; OHCA) treated with ECPR. We performed a systematic review with meta-analysis. The f...

In den meisten Fällen geht es bei statistischen Datenanalysen darum, gewisse unbekannte Kenngrößen einer Population (auch Grundgesamtheit genannt) zu bestimmen. In der Statistik nennt man dies Schätzen oder Fitten unbekannter Parameter. Beispiele sind etwa Wahrscheinlichkeiten, Mittelwerte, Mediane, Standardabweichungen, Korrelationen oder auchPara...

Zusammenfassung Hintergrund Körperliches Training und antiresorptive pharmakologische Therapie wirken über unterschiedliche Mechanismen auf den Knochenstoffwechsel ein. Die vorliegende Arbeit beschäftigt sich mit dem Ansatz, ob eine Bisphosphonat-Behandlung durch zusätzliches körperliches Training additive Effekte auf die Knochendichte (BMD) an Len...

Evidence regarding perfusion conditions during extracorporeal cardiopulmonary resuscitation (ECPR) is rare. Therefore, we investigated the impact of perfusion parameters on neurologic outcome and survival in patients with in- or out-of-hospital cardiac arrest (IHCA; OHCA) treated with ECPR. We performed a systematic review with meta-analysis. The f...

Zusammenfassung Hintergrund Die Transfusion von Erythrozytenkonzentraten (EK) ist mit verschiedenen Nebenwirkungen assoziiert, die u. a. durch Lagerungsschäden an Erythrozyten hervorgerufen werden. Die Zellen verändern ihre Struktur und setzen dabei Kalium sowie Lactat frei. Zur Minimierung dieser negativen Effekte können die Erythrozyten mithilfe...

The purpose of this systematic review and meta‐analysis (PROSPERO ID: CRD42021250467) was to evaluate the effects of exercise on low‐trauma overall and major osteoporotic fractures (hip, spine, forearm or humerus fractures) and to determine the corresponding effect of supervision of the exercise program. Our systematic search of six literature data...

In this study, relevant variables were determined to predict the probability of sooty mould biofilm (SMB) occurrence on willow leaves in a multivariate model. Despite a large temporal gap between the main sap-feeding insect (SFI) season in spring (May to June) and the main season of SMBs in late summer and fall (August to September), trees infested...

Background. Chest compresses with mustard (MU) or ginger (GI) are a complementary treatment option for respiratory tract infections. However, little is known about their specific thermogenic qualities. This study examines the short-term effects of MU, GI, and chest compresses with warm water only (WA) on measurable and self-perceived body warmth in...

Der Ausgangspunkt für eine effiziente und fehlerfreie statistische Analyse ist eine adäquate Erhebung, Organisation und Speicherung der Daten. Dabei sollte das Ziel eines guten Datenmanagements immer sein, die FAIR-Prinzipien („findable, accessible, interoperable, reusable“) einzuhalten [1]. Darüber hinaus sollten personenbezogene Daten immer in ps...