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Adequate functional recovery after a nerve injury reconstructed with a long nerve graft, tissue-engineered construct, or scaffold is rarely achieved. This outcome is due to few axons regenerating across the bridging material. The reason as to why the bridging material can support robust axon regeneration across short distances/lengths and not longer distances is poorly understood. This project seeks to understand the biology of nerve regeneration across long scaffolds and utilizes bioengineering techniques and approaches.