Matthew J Stasiewicz

Matthew J Stasiewicz
University of Illinois, Urbana-Champaign | UIUC · Department of Food Science and Human Nutrition

PhD

About

34
Publications
9,041
Reads
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1,292
Citations
Additional affiliations
August 2015 - present
University of Illinois, Urbana-Champaign
Position
  • Upcoming Assistant Professor
Description
  • I will be starting in August
January 2008 - May 2015
Cornell University
Position
  • PhD Student
August 2004 - May 2007
Michigan State University
Position
  • Research Assistant
Description
  • I worked with Dr. Brad Marks on the effect of sublethal heating on the subsequent heat tolerance of Salmonella

Publications

Publications (34)
Article
The U.S. Department of Agriculture, Food Safety and Inspection Service maintains a recall case archive of meat and poultry product recalls from 1994 to the present. In this study, we collected all recall records from 1994 to 2015 and extracted the recall date, meat or poultry species implicated, reason for recall, recall class, and pounds of produc...
Article
Maize, a staple food in many African countries including Kenya, is often contaminated by toxic and carcinogenic fungal secondary metabolites such as aflatoxins and fumonisins. This study evaluated the potential use of a low-cost, multi-spectral sorter in identification and removal of aflatoxin- and fumonisin-contaminated single kernels from a bulk...
Article
While the foodborne pathogen Listeria monocytogenes can persist in food associated environments, there are no whole genome sequence (WGS) based methods to differentiate persistent from sporadic strains. Whole genome sequencing of 188 isolates from a longitudinal study of L. monocytogenes in retail delis was used to (i) apply single nucleotide polym...
Article
Aflatoxin and fumonisin contamination distribution in corn is non-homogeneous. Therefore, bulk sample testing may not accurately represent the levels of contamination. Single kernel analysis could provide a solution to these problems and lead to remediation strategies such as sorting. Our study uses extensive single kernel aflatoxin (AF) and fumoni...
Article
Full-text available
A prophage is a phage-related sequence that is integrated into a bacterial chromosome. Prophages play an important role in bacterial evolution, survival, and persistence. To understand the impact of Listeria prophages on their host genome organizations, this work sequenced two L. monocytogenes strains (134LM and 036LM), previously identified as lys...
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Probe sampling plans for aflatoxin in corn attempt to reliably estimate concentrations in bulk corn given complications like skewed contamination distribution and hotspots. To evaluate and improve sampling plans, three sampling strategies (simple random sampling, stratified random sampling, systematic sampling with U.S. GIPSA sampling schemes), thr...
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Ready-to-eat meat products, such as deli ham, can support the growth of Listeria monocytogenes (LM), which can cause severe illness in immunocompromised individuals. The objectives of this study were to validate a miniature ham model (MHM) against the ham slice method and to screen antimicrobial combinations to control LM on ham by using response s...
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Full-text available
While complex sample pooling strategies have been developed for large-scale experiments with robotic liquid handling, many medium-scale experiments like mycotoxin screening by Enzyme-Linked Immunosorbent Assay (ELISA) are still conducted manually in 48-and 96-well plates. At this scale, the opportunity to save on reagent costs is offset by the incr...
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Full-text available
Current detection methods for contamination of aflatoxin and fumonisin used in the corn industry are based on bulk level. However, literature demonstrates that contamination of these mycotoxins is highly skewed and bulk samples do not always represent accurately the overall contamination in a batch of corn. Single kernel analysis can provide an ins...
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Full-text available
Background: The foodborne pathogen Listeria monocytogenes causes the potentially lethal disease listeriosis. Within food-associated environments, L. monocytogenes can persist for long periods and increase the risk of contamination by continued presence in processing facilities or other food-associated environments. Most research on phenotyping of...
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Full-text available
We report here the complete genome sequences of three Listeria phages (PSU-VKH-LP019, PSU-VKH-LP040, and PSU-VKH-LP041), which were newly induced from lysogenic isolates of Listeria monocytogenes from seafood and a seafood processing environment in Thailand. The three phages show circularly permuted double-stranded DNA genomes with sizes of 38.6, 3...
Article
Queso Fresco (QF), a fresh Hispanic-style cheese, is often associated with Listeria monocytogenes outbreaks and recalls. Queso Fresco's susceptibility to bacterial contamination is partially due to its high pH and moisture content as well as Listeria's tolerance for the salt content typical for QF. Nine different brands of US QF, 2 packages from 4...
Article
In a recent longitudinal surveillance study in 30 U.S. retail delis, we found 9.7% of environmental surfaces positive for Listeria monocytogenes and substantial evidence of persistence. In this study, we aimed to reduce the prevalence and persistence of L. monocytogenes in the retail deli environment by developing and implementing practical and fea...
Article
Pathogen environmental monitoring programs (EMPs) are essential for food processing facilities of all sizes that produce ready-To-eat food products exposed to the processing environment. We developed, implemented, and evaluated EMPs targeting Listeria spp. and Salmonella in nine small cheese processing facilities, including seven farmstead faciliti...
Article
Genomics tools, and specifically whole-genome sequencing (WGS) methods, are rapidly transforming food safety, specifically through improved outbreak detection. In the U.S., whole genome sequencing of all human clinical Listeria monocytogenes isolates has been performed since 2013; isolates from foods and food processing plants obtained by regulator...
Article
Career and educational opportunities in food science and food safety are underrecognized by K-12 students and educators. Additionally, misperceptions regarding nature of science understanding persist in K-12 students despite being emphasized as an important component of science education for over 100 y. In an effort to increase awareness concerning...
Article
Full-text available
Listeria monocytogenes is a foodborne pathogen that causes an estimated 1,591 cases of illness and 255 deaths annually in the United States, the majority of which are attributed to ready-to-eat deli meats processed in retail delis. Because retail delis distribute product directly to consumers, rapid methods to validate cleaning and sanitation are n...
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Postprocessing contamination in processing plants has historically been a significant source of Listeria monocytogenes in ready-to-eat delicatessen meats, and therefore a major cause of human listeriosis cases and outbreaks. Recent risk assessments suggest that a majority of human listeriosis cases linked to consumption of contaminated deli meats m...
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In a 2005 analysis of a potential bioterror attack on the food supply involving a botulinum toxin release into the milk supply, the authors recommended adopting a toxin inactivation step during milk processing. In response, some dairy processors increased the times and temperatures of pasteurization well above the legal minimum for high temperature...
Article
Increasing evidence suggests that persistence of Listeria monocytogenes in food processing plants has been the underlying cause of a number of human listeriosis outbreaks. This study extracts criteria used by food safety experts in determining bacterial persistence in the environment, using retail delicatessen operations as a model. Using the Delph...
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Full-text available
Sampling of agricultural and natural environments in two US states (Colorado and Florida) yielded 18 Listeria-like isolates that could not be identified as previously described species using traditional methods. Using whole genome sequencing and traditional phenotypic methods we identified five new species, each with a genome wide average blast nuc...
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Full-text available
Over the last 10 to 15 years, increasing evidence suggests that persistence of Listeria monocytogenes in food processing plants for years or even decades is an important factor in the transmission of this foodborne pathogen and the root cause of a number of human listeriosis outbreaks. L. monocytogenes persistence in other food-associated environme...
Article
Contamination of Ready-To-Eat foods with Listeria monocytogenes can typically be traced back to post-processing contamination from environmental sources; contamination is often linked to subtypes that persist in food associated environments. Although phage-based biocontrol strategies have been proposed for controlling this pathogen, information on...
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Full-text available
Listeria monocytogenes persistence in food processing plants is a key source of postprocessing contamination of ready-to-eat foods. Thus, identification and elimination of sites where L. monocytogenes persists (niches) is critical. Two smoked fish processing plants were used as models to develop and implement environmental sampling plans (i) to ide...
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The organic acids lactate and diacetate are commonly used in combination in ready-to-eat foods because they show synergistic ability to inhibit the growth of Listeria monocytogenes. Full-genome microarrays were used to investigate the synergistic transcriptomic responses of two L. monocytogenes strains, H7858 (serotype 4b) and F6854 (serotype 1/2a)...
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Full-text available
The persistence of Listeria monocytogenes in food-associated environments represents a key factor in transmission of this pathogen. To identify persistent and transient strains associated with production of fermented meat sausages in northern Portugal, 1,723 L. monocytogenes isolates from raw material and finished products from 11 processors were i...
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Full-text available
Combinations of organic acids are often used in ready-to-eat foods to control the growth of Listeria monocytogenes during refrigerated storage. The purpose of this study was to quantitatively assess synergy between two organic acid growth inhibitors under conditions similar to those present in cold-smoked salmon, and to assess the effect of evoluti...
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Full-text available
Traditional models for predicting the thermal inactivation rate of bacteria are state dependent, considering only the current state of the product. In this study, the potential for previous sublethal thermal history to increase the thermotolerance of Salmonella in ground turkey was determined, a path-dependent model for thermal inactivation was dev...

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Projects (2)
Project
We aim to simulate sampling in a 2D field or 3D space contaminated with foodborne pathogens or mycotoxins to improve the effectiveness of current sampling plans.