Matthew R Clark

• Founder at Polymyositis Research Association

MHC imbalance can cause attack by T-cells in certain diseases. Wondering if the MHC balance was affected by cellular glutathione levels?

Can ALA be reduced to a salt? Will it lose its effectiveness for lengthening telomeres?

I have a client that cannot swallow the pills, but it is too strong to take as powder, even mixed in liquid. I would like to make it into a salt if this can be done without it losing its effectiveness.

Can they be taken close together without loss of effect? How much time if any must separate oral administration to avaoid any loss of efficacy of the serrapeptase?

Recently I was reading about the structure of musculature with a focus on the difficulties of muscular regeneration in polymyositis. My attention was drawn to the mention that the stem cells for the regeneration of muscle were located in the luminal / sub luminal layer of the muscle. This is probably well-known, but I had not really considered it before.

It reminded me of the excellent article by Drs. Schubert, Masters et al Entitled “APP+ T lymphocytes selectively sorted to endomysial tubes in polymyositis displace NCAM-expressing muscle fibers”.[1] In this article the what interested me was the suggestion that a combined expression of CDT8+, CD4+, and APP+ may form as an invasive front or ‘attack complex.

What particularly struck my notice was the statement that the attack complex so formed attacked the lamina layer in particular, effectively destroying the NCAM adhesion molecules and loosening the endomysial basal lamina, displacing the muscle fibers. The idea is interesting, and I wonder about the effect of this proposed attack complex on the muscular stem cells.

Is it possible that muscular regeneration in polymyositis is thwarted at least in part due to the disruption of the stem cells from their natural habitat by the attack complex targeting the stem cells home in the basal lamina?

1. Schubert W, Masters CL, Beyreuther K, APP+ T lymphocytes selectively sorted to endomysial tubes in polymyositis displace NCAM-expressing muscle fibers., European Journal of Cell Biology [1993, 62(2):333-342] as found at http://europepmc.org/abstract/med/7925489

The anti-oxidant properties of a-lipoic acid are well established, but the taste is very strong for oral administration unless capsulated.  For patients with dysphagia who cannot swallow capsules, is there a preferred method or carrier for oral administration that will not interfere with its function but will render it palatable for the patient?

We are looking at DAMP in its role as mediator of inflammatory cytokines in muscle tissue. What is your preferred method for determining DAMP level?

What markers have you had success with in measuring ER Stress in human subjects?