Matteo Marzi

Matteo Marzi
Istituto Italiano di Tecnologia | IIT · Center for Genomic Science

Phd in Molecular Medicine

About

49
Publications
14,126
Reads
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1,184
Citations
Citations since 2017
38 Research Items
925 Citations
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Introduction
I'm a biotechnologist who has trained in genomic science, with over 10 years of experience in non-coding RNA Biology. My main interest is in the role of ncRNAs in differentiation and in cancer and, more specifically, in their ability to modify the expression of genes, shaping cell fate. My scientific approach is characterized by the exploitation of genome-wide approaches, novel technologies and bioinformatics.
Additional affiliations
April 2011 - October 2012
IEO - Istituto Europeo di Oncologia
Position
  • PostDoc Position
January 2006 - December 2011
IEO - Istituto Europeo di Oncologia
Position
  • microRNA in the control of quiescence and differentiation
Education
January 2006 - January 2010
University of Milan
Field of study
  • Molecular Medicine
September 2001 - July 2006
University of Milan
Field of study
  • Biotechnology

Publications

Publications (49)
Chapter
MicroRNAs (miRNAs) are an evolutionarily conserved class of small (18–22 nucleotides) noncoding RNAs involved in the regulation of a variety of cellular and developmental processes. MiRNA expression is frequently altered in human cancers compared to normal tissues, potentially contributing to tumorigenesis. Generally, high-throughput profiles of mi...
Article
Full-text available
The transition from dividing progenitors to postmitotic motor neurons (MNs) is orchestrated by a series of events, which are mainly studied at the transcriptional level by analyzing the activity of specific programming transcription factors. Here, we identify a post-transcriptional role of a MN-specific transcriptional unit (MN2) harboring a lncRNA...
Article
Full-text available
It is currently unknown how many RNA transcripts are able to induce degradation of microRNAs (miRNA) via the mechanism known as target-directed miRNA degradation (TDMD). We developed TDMDfinder, a computational pipeline that identifies ‘high confidence’ TDMD interactions in the Human and Mouse transcriptomes by combining sequence alignment and feat...
Article
Full-text available
The original version of this Article contained an error in the spelling of the author Marco Bacigaluppi, which was incorrectly given as Marco Bagicaluppi. This has now been corrected in both the PDF and HTML versions of the Article.
Article
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Recent findings in human samples and animal models support the involvement of inflammation in the development of Parkinson's disease. Nevertheless, it is currently unknown whether microglial activation constitutes a primary event in neurodegeneration. We generated a new mouse model by lentiviral-mediated selective α-synuclein (αSYN) accumulation in...
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Although ectopic overexpression of miRNAs can influence mammary normal and cancer stem cells (SCs/CSCs), their physiological relevance remains uncertain. Here, we show that miR-146 is relevant for SC/CSC activity. MiR-146a/b expression is high in SCs/CSCs from human/mouse primary mammary tissues, correlates with the basal-like breast cancer subtype...
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Friedreich’s ataxia (FRDA) is an autosomal-recessive neurodegenerative and cardiac disorder which occurs when transcription of the FXN gene is silenced due to an excessive expansion of GAA repeats into its first intron. Herein, we generate dorsal root ganglia organoids (DRG organoids) by in vitro differentiation of human iPSCs. Bulk and single-cell...
Article
Full-text available
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
Data
Supplemental Information MiR-135a-5p Is Critical for Exercise-Induced Adult Neurogenesis Meritxell Pons-Espinal, Caterina Gasperini, Matteo J. Marzi, Clarissa Braccia, Andrea Armirotti, Alexandra Pötzsch, Tara L. Walker, Klaus Fabel, Francesco Nicassio, Gerd Kempermann, and Davide De Pietri Tonelli
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Physical exercise stimulates adult hippocampal neurogenesis and is considered a relevant strategy for preventing age-related cognitive decline in humans. The underlying mechanisms remains controversial. Here, we show that exercise increases proliferation of neural precursor cells (NPCs) of the mouse dentate gyrus (DG) via downregulation of microRNA...
Article
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Long noncoding RNAs (lncRNAs) are emerging as regulators of fundamental biological processes. Here we report on the characterization of an intergenic lncRNA expressed in epithelial tissues which we termed EPR (Epithelial cell Program Regulator). EPR is rapidly downregulated by TGF-β and its sustained expression largely reshapes the transcriptome, f...
Article
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The role of the tumour-suppressor miR-34 family in breast physiology and in mammary stem cells (MaSCs) is largely unknown. Here, we revealed that miR-34 family, and miR-34a in particular, is implicated in mammary epithelium homoeostasis. Expression of miR-34a occurs upon luminal commitment and differentiation and serves to inhibit the expansion of...
Article
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Little is known about miRNA decay. A target-directed miRNA degradation mechanism (TDMD) has been suggested, but further investigation on endogenous targets is necessary. Here, we identify hundreds of targets eligible for TDMD and show that an endogenous RNA (Serpine1) controls the degradation of two miRNAs (miR-30b-5p and miR-30c-5p) in mouse fibro...
Chapter
MicroRNAs (miRNAs) are an evolutionary conserved class of short, single-stranded noncoding RNAs (<18-22 nt in length) that act in posttranscriptional regulation of gene expression in higher eukaryotes. The abundance of a miRNA is a key feature in control of its activity and, therefore, a number of mechanisms finely regulate miRNA levels, acting at...
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Full-text available
Adult neurogenesis requires the precise control of neuronal versus astrocyte lineage determination in neural stem cells. While microRNAs (miRNAs) are critically involved in this step during development, their actions in adult hippocampal neural stem cells (aNSCs) has been unclear. As entry point to address that question we chose DICER, an endoribon...
Data
Table S1. Predicted Targets of at Least Two of the 11 miRNAs Selected with Mirwalk 2.0, Related to Figure 5
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Recent evidence indicates that the miRNA biogenesis factors DROSHA, DGCR8, and DICER exert non‐overlapping functions, and have also roles in miRNA‐independent regulatory mechanisms. However, it is currently unknown whether miRNA‐independent functions of DGCR8 play any role in the maintenance of neuronal progenitors and during corticogenesis. Here,...
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BACKGROUND: The identification of circulating microR- NAs (miRNAs) in the blood has been recently exploited for the development of minimally invasive tests for the early detection of cancer. Nevertheless, the clinical transferability of such tests is uncertain due to still-insufficient standard- ization and optimization of methods to detect circula...
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The regulation of miRNAs is critical to the definition of cell identity and behavior in normal physiology and disease. To date, the dynamics of miRNA degradation and the mechanisms involved in remain largely obscure, in particular, in higher organisms. Here, we developed a pulse-chase approach based on metabolic RNA labeling to calculate miRNA deca...
Article
Lung cancer is leading cause of cancer death worldwide. Being lung cancer asymptomatic in its early stages, the majority of patients are diagnosed with advanced disease. Therefore, it is vital that screening programs and novel diagnostic tools are developed to increase lung cancer early detection. The development of a minimally invasive blood-based...
Article
Lung cancer is the leading cause of cancer death worldwide. Low-dose computed tomography screening (LDCT) was recently shown to anticipate the time of diagnosis, thus reducing lung cancer mortality. However, concerns persist about the feasibility and costs of large-scale LDCT programs. Such concerns may be addressed by clearly defining the target "...
Article
Proceedings: AACR Annual Meeting 2014; April 5-9, 2014; San Diego, CA Background. The recent development of high-throughput sequencing technologies (Next Generation Sequencing - NGS) provided instruments to reveal the complexity of nucleic acids, highlighting the existence of numerous species of non-coding RNAs (ncRNAs) that play a significant reg...
Article
Cancer stem cells (CSCs) are cells with aberrantly regulated stem-like characteristics, such as self-renewal and quiescence. In breast cancer, CSCs have been shown to drive the tumorigenic process and to contribute to the emergence of therapeutic resistance and disease relapse. Based on this background, we decided to focus on the mechanisms that su...
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Full-text available
As more small RNA sequencing libraries are becoming available, it clearly emerges that microRNAs (miRNAs) are highly heterogeneous both in length and sequence. In comparison to canonical miRNAs, miRNA isoforms (termed as "isomiRs") might exhibit different biological properties, such as a different target repertoire, or enhanced/reduced stability. N...
Article
Proceedings: AACR 104th Annual Meeting 2013; Apr 6-10, 2013; Washington, DC Background. High incidence and mortality rate tumours present a more favourable prognosis if diagnosed in early stages (Jemal A. et al, 2011). Screening programs based on effective radiological techniques (e.g. mammography or low dose spiral CT) promise a mortality reducti...
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Full-text available
The cancer-associated loss of microRNA (miRNA) expression leads to a proliferative advantage and aggressive behavior through largely unknown mechanisms. Here, we exploit a model system that recapitulates physiological terminal differentiation and its reversal upon oncogene expression to analyze coordinated mRNA/miRNA responses. The cell cycle reent...
Article
Full-text available
Lung cancer is the first cause of cancer mortality worldwide, and its early detection is currently the main available strategy to improve disease prognosis. While early diagnosis can be successfully achieved through tomography-based population screenings in high-risk individuals, simple methodologies are needed for effective cancer prevention progr...

Questions

Question (1)
Question
We are experiencing a problem when digesting the mouse mammary gland. At the end of the procedure (that is the one published by Weinberg group; digestion with collagenase+ialuronidase+dispase/DNAse), we find, a huge number of "objects" (I use objects since I am unaware if they are of biological origin) mostly in the shape of rectangles (like sheets). This stuff cannot be eliminated by pelleting or by filtering at 40um. Since we grow cells in suspension, we cannot even eliminate this contamination by cell adhesion. I know it is difficult, but is there anyone that can suggest the origin of the contamination, or a smart way of getting rid of them? If I can I will attach a photo to make it more clearer.

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Projects

Project (1)
Project
Uncover the role of small noncoding RNAs, such as microRNAs, in mammalian neurogenesis and astrocyte circadian rhythms. Since 2016 we are also investigating alternative miRNA-independent functions of the Microprocessor protein Dgcr8. Since 2017 we are using miRNAs as potential drugs. miRNAs are administered in vitro/vivo via nanoparticles (available at IIT in collaboration with the lab of Paolo Decuzzi's and Nicola Tirelli's labs. Targets are Glioblastoma and Neuro-protection)