Mathieu Rolland

Mathieu Rolland
Agence Nationale de Sécurité Sanitaire de l'Alimentation, de l'Environnement et du Travail | ANSES · ANSES Plant Health Laboratory

PhD

About

41
Publications
7,026
Reads
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206
Citations
Additional affiliations
May 2016 - present
Agence Nationale de Sécurité Sanitaire de l'Alimentation, de l'Environnement et du Travail
Position
  • Head of Department
March 2011 - May 2016
Le Groupe d'Etude et de contrôle des Variétés Et des Semences.
Position
  • Head of Department
January 2009 - December 2010
Cornell University
Position
  • Post doctoral associate
Description
  • Aphid cell and molecular biology in virus transmission
Education
March 2005 - December 2008
Agrocampus Ouest
Field of study
  • Plant Virology
September 2002 - October 2004
Agrocampus Ouest
Field of study
  • Plant protection
September 2000 - June 2002
University of Angers
Field of study
  • Plant science

Publications

Publications (41)
Article
Full-text available
Background: The European Community has adopted very restrictive policies regarding the dissemination and use of genetically modified organisms (GMOs). In fact, a maximum threshold of 0.9% of contaminating GMOs is tolerated for a "GMO-free" label. In recent years, imports of undescribed GMOs have been detected. Their sequences are not described and...
Article
Full-text available
The presence of genetically modified organisms (GMO) is commonly assessed using real-time PCR methods targeting the most common transgenic elements found in GMOs. Once the presence of GM material has been established using these screening methods, GMOs are further identified using a battery of real-time PCR methods, each being specific of one GM ev...
Article
Full-text available
The market of ornamental plants is extremely competitive, and for many species genetic engineering can be used to introduce original traits of high commercial interest. However, very few genetically modified (GM) ornamental varieties have reached the market so far. Indeed, the authorization process required for such plants has a strong impact on th...
Article
Full-text available
The EU directive 2001/18/EC requires any genetically modified (GM) event to be stable. In the present work, a targeted Next-Generation Sequencing (NGS) approach using barcodes to specifically tag each individual DNA molecules during library preparation was implemented to detect mutations taking into account the background noise due to amplification...
Article
Full-text available
Despite the generalized use of cultivars carrying the rym4 resistance gene, the impact of viral mosaic diseases on winter barleys increased in recent years in France. This change could reflect i) an increased prevalence of the rym4 resistance-breaking pathotype of Barley yellow mosaic virus Y (BaYMV-2), ii) the emergence of rym4 resistance-breaking...
Data
Accession numbers of sequences deposited in Genbank. (DOCX)
Data
Information on MySeq multiplexed runs, sequencing reads and reads mapping to BaYMV VPg for the various samples analyzed in this study. (DOCX)
Conference Paper
Full-text available
Objectives: Despite the widespread use of resistant varieties carrying the rym4 gene, the recent years have seen an increase in the impact of viral mosaic diseases in winter barley crops in France. Surveys showed that yield losses could reach up to 20% in some cases. This novel worrisome situation could result from a change in the populations of th...
Conference Paper
Full-text available
Genetic resistance is the only way to control mosaic damage in winter barley. At least 18 resistance genes are known, but until recently, rym4 was the most used by breeders to control Barley yellow mosaic virus-1 (BaYMV-1), predominant in France and Barley mild mosaic virus (BaMMV). However, since 2009, new significant damage has been observed in m...
Article
Full-text available
Broomrapes are holoparasitic plants spreading through seeds. Each plant produces hundreds of thousands of seeds which remain viable in the soils for decades. To limit their spread, drastic measures are being taken and the contamination of a commercial seed lot by a single broomrape seed can lead to its rejection. Considering that broomrapes species...
Data
Alignment of partial sequences of the trnL gene obtained by amplification using the primers trnL C (F) and trnL HRM R of total DNA extracted from individual seeds belonging to the 8 species of interest.
Data
Alignment of partial sequences of the rbcL gene obtained by amplification of total DNA extracted from individual seeds belonging to 7 of the 8 species of interest.
Article
Winter barley is subjected to severe yield losses due to the yellow mosaic virus disease. Two soil borne bymoviruses, BaYMV (Barley yellow mosaic virus) and BaMMV (Barley mild mosaic virus) are responsible for this disease in Europe. As breeding resistant cultivars is the only control method against the disease, barley varieties carrying the recess...
Technical Report
Full-text available
Testing seed lots for the unintended presence of genetically modified (GM) seeds is carried out in European Union Member States (MS). The aim of the testing of seeds for genetically modified organisms (GMOs) is to test whether GMOs are present in non-GM seed lots. Splitting samples of seeds taken from lots into subsamples, testing for the presence...
Article
Full-text available
Endornaviruses are unusual plant-, fungus- and oomycete-infecting viruses with a large, ca 14- to 17-kb linear double-stranded RNA (dsRNA) genome and a persistent lifestyle. The complete genome sequence of an endornavirus from the barley (Hordeum vulgare) Nerz variety was determined from paired Illumina MySeq reads derived from purified dsRNAs. The...
Article
Clavibacter michiganensis subsp. michiganensis (Cmm) is one of the most important seed-transmitted pathogens in tomato. In recent years, the number of outbreaks has increased. Due to the absence of chemical controls, the most effective way to prevent the spread of this disease is through the use of clean seed, requiring effective seed health testin...
Article
Full-text available
QUALITÉ 9 M aladies virales transmises par un champignon du sol appelé Polymyxa grami-nis, les mosaïques du blé peuvent entraîner des pertes de rende-ments allant de 20 à 80 % en blé tendre et jusqu'à 100 % en blé dur. Il en existe deux types pro-voquées par deux virus à ARN : la mosaïque des céréales est due au VMC (virus de la mosaïque des céréal...
Article
Understanding the processes that have led to the recent prevalence of necrotic genotypes in PVY populations is an important challenge for research programs studying this virus. Non-necrotic PVY(O)-139, necrotic PVY(N)-605 and point mutated versions of PVY(N)-605 (PVY(KRED), PVY(KR) and PVY(ED)), were used in mixtures to inoculate two Nicotiana host...
Article
Homology-driven proteomics promises to reveal functional biology in insects with sparse genome sequence information. A proteomics study comparing plant virus transmission competent and refractive genotypes of the aphid Schizaphis graminum isolated numerous candidate proteins involved in virus transmission, but limited genome sequence information ha...
Chapter
The analysis of viral populations requires the use of techniques that describe characteristics of individuals. The single-strand conformation polymorphism (SSCP) makes possible the identification of genetic differences between viral sequences and constitutes an alternative to the expensive and time-consuming cloning and sequencing strategies. Appli...
Article
The prevalence of necrotic potato virus Y (PVY) in natural populations could reflect increased fitness of necrotic isolates. In this paper, the effects of the acquisition of molecular determinants (A/G(2213) and A/C(2271)) involved in necrosis capacity on both the number of progeny produced and the competitiveness of PVY were characterized. The rel...
Article
Fluorescent-based technologies offer opportunities for developing new assays for detection, quantification, and characterization of viral isolates. According to the intrinsic characteristics of fluorescent-based tools (high specificity, sensitivity, and reliability), such type of molecular assays makes possible investigations on original studies su...
Article
The analysis of viral populations requires the use of techniques that describe characteristics of individuals. The single-strand conformation polymorphism (SSCP) makes possible the identification of genetic differences between viral sequences and constitutes an alternative to the expensive and time-consuming cloning and sequencing strategies. Appli...
Article
Full-text available
Vous trouverez ci-après le tiré à part de votre article en format électronique (pdf) : Le virus Y de la pomme de terre (PVY) : de la première description aux derniers épisodes d'émer-gence paru dans Virologie, 2008, Volume 12, Numéro 4 John Libbey Eurotext Ce tiré à part numérique vous est délivré pour votre propre usage et ne peut être transmis à...
Article
Full-text available
The Potato Virus Y (PVY) is one of the most economically important potato pathogens. PVY isolates are described according to their biological, serological and molecular characteristics. Such data have been used to classify PVY isolates in groups (including the PVY N and PVY O main groups) and variants (PVY N-W (also described as PVY N:O) and PVY NT...
Article
The complex Potato virus Y classification, including groups (PVYN and PVYO) and variants (PVYNTN and PVYN-W), is based mainly on biological properties of isolates. Published PVY detection tools targeting markers not associated with biological properties could fail to assign correctly isolates in the current classification. To improve PVY detection...
Article
Full-text available
plant pathogens. First described in the 1930s as a pathogen of potato, PVY infects a wide host range mainly within the Solanaceae and is distributed worldwide. This virus is transmitted by aphids in a non-persistent manner and has a single-stranded, positive-sense RNA genome of about 10 kb in length that encodes a large polyprotein cleaved into nin...
Chapter
Fluorescent-based technologies offer opportunities for developing new assays for detection, quantification, and characterization of viral isolates. According to the intrinsic characteristics of fluorescent-based tools (high specificity, sensitivity, and reliability), such type of molecular assays makes possible investigations on original studies su...

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