Mateusz Kucharczyk

Mateusz Kucharczyk
King's College London | KCL · Wolfson Centre for Age-Related Diseases

BSc, MSc, PhD

About

33
Publications
4,586
Reads
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337
Citations
Introduction
Mateusz currently works in the Bannister's Lab, Wolfson Centre for Age-Related Diseases, King's College London. Mateusz does research in Biophysical Neuroscience and Pharmacology. He is interested in pain perception and what defines it. In particular he explores how does the brain control nociception by top-down modulation. Currently he studies that in the context of neuropathic and cancer pain. See more at: www.mateuszk.com
Additional affiliations
May 2019 - present
King's College London
Position
  • PostDoc Position
Description
  • Funded by the Academy of Medical Sciences and the Wellcome Trust I currently work in the Central Modulation of Pain Group lead by Dr Kirsty Bannister.
April 2017 - May 2018
King's College London
Position
  • Fellow
Description
  • A secondment within BonePain Network in Professor Stephen McMahon's Lab. Training in in vivo GCaMP6s imaging in sensory ganglia.
October 2015 - April 2019
University College London
Position
  • Researcher
Description
  • A project under the Bone Pain Network (www.bonepain.eu) covers in vivo electrophysiological recordings supplied with pharmacological tools to dissect peripheral and central consequences of cancer-induced bone pain.
Education
October 2015 - May 2019
University College London
Field of study
  • Neuroscience
October 2012 - June 2014
Jagiellonian University
Field of study
  • Biotechnology
October 2009 - July 2012
Jagiellonian University
Field of study
  • Biotechnology

Publications

Publications (33)
Article
Objective: Develop and validate a low‐intensity sinusoidal electrical stimulation paradigm to preferentially activate C‐fibers in human skin. Methods: Sinusoidal transcutaneous stimulation (4 Hz) was assessed psychophysically in healthy volunteers (n=14) and neuropathic pain patients (n=9). Pursuing laser Doppler imaging and single nociceptor recor...
Article
In the present study, we asked if the different types of stress alter neuronal plasticity markers distinctively in the frontal cortex (FCx) and in the hippocampus (Hp). To do so, we implemented various stress regimens to analyze changes evoked in these rat brain structures. We utilized several molecular techniques, including western blot, ELISA, qu...
Article
Full-text available
Background: The term 'irritable nociceptor' was coined to describe neuropathic patients characterized by evoked hypersensitivity and preservation of primary afferent fibres. Oxcarbazepine is largely ineffectual in an overall patient population, but has clear efficacy in a subgroup with the irritable nociceptor profile. We examine whether neuropath...
Article
Full-text available
Skeletal metastases are frequently accompanied by chronic pain that is mechanoceptive in nature. Mechanistically, cancer-induced bone pain (CIBP) is mediated by peripheral sensory neurones innervating the cancerous site, the cell bodies of which are housed in the dorsal root ganglia (DRG). How these somatosensory neurons encode sensory information...
Article
Brainstem to spinal cord noradrenergic pathways include a locus coeruleus origin projection and diffuse noxious inhibitory controls. While both pathways are traditionally viewed as exerting an inhibitory effect on spinal neuronal activity, the locus coeruleus was previously shown to have a facilitatory influence on thermal nociception according to...
Preprint
Full-text available
Brainstem to spinal cord pathways modulate spinal neuronal activity. We implemented locus coeruleus (LC) targeting strategies by microinjecting CAV-PRS-ChR2 virus in the spinal cord (LC:SC module) or LC (LC:LC module). While activation of both modules inhibited evoked spinal neuronal firing via α1-adrenoceptor-mediated actions, LC:SC opto-activatio...
Article
Full-text available
Bulbospinal pathways regulate nociceptive processing, and inhibitory modulation of nociception can be achieved via the activity of diffuse noxious inhibitory controls (DNIC), a unique descending pathway activated upon application of a conditioning stimulus (CS). Numerous studies have investigated the effects of varied pharmacological systems on the...
Article
Full-text available
Pain resulting from metastatic bone disease is a major unmet clinical need. Studying spinal processing in rodent models of cancer pain is desirable since the percept of pain is influenced in part by modulation at the level of the transmission system in the dorsal horn of the spinal cord. Here, a rodent model of cancer-induced bone pain (CIBP) was g...
Preprint
Full-text available
Pain resulting from metastatic bone disease is a major unmet clinical need. Studying spinal processing in rodent models of cancer pain is desirable since the percept of pain is influenced in part by modulation at the level of the transmission system in the dorsal horn of the spinal cord. Here a rodent model of cancer induced bone pain (CIBP) was ge...
Article
Full-text available
Background Diffuse noxious inhibitory controls (DNIC) as measured in rat and conditioned pain modulation (CPM), the supposed psychophysical paradigm of DNIC measured in humans, are unique manifestations of an endogenous descending modulatory pathway that is activated by application of a noxious conditioning stimulus. The predictive value of the hum...
Thesis
Cancer pain remains a major area of unmet medical need. One common form of chronic cancer pain, affecting 400,000 people each year in the US alone, is associated with skeletal metastases. These pains are typically mechanoceptive in nature and poorly managed by available analgesics. Here, I employed in vivo imaging using GCaMP6s to assess the proper...
Preprint
Full-text available
Cancer pain remains a major area of unmet medical need, with few studies existing in proportion to that need. One common form, affecting 400,000 people each year in the US alone, is associated with skeletal metastases. These pains are typically mechanoceptive in nature and poorly managed by available analgesics. Here, we employed in vivo imaging us...
Article
Background: In depression, excessive glucocorticoid action may cause maladaptive brain changes, including in the pathways controlling energy metabolism. Insulin and glucagon-like peptide-1 (GLP-1), besides regulation of glucose homeostasis, also possess neurotrophic properties. Current study was aimed at investigating the influence of prenatal str...
Article
Here we aim to present an accessible review of the pharmacological targets for pain management, and succinctly discuss the newest trends in pain therapy. A key task for current pain pharmacotherapy is the identification of receptors and channels orchestrating nociception. Notwithstanding peripheral alterations in the receptors and channels followin...
Article
Full-text available
Elevated levels of glucocorticoids exert neurotoxic effects, and the hippocampus is particularly sensitive to the effects of glucocorticoids. Because some data have indicated that an increased action of glucocorticoids in the perinatal period enhances the susceptibility of brain tissue to adverse substances later in life, the main purpose of the pr...
Article
Several lines of evidence indicate that adverse experience in early life may be a triggering factor for pathological inflammatory processes and lead to the development of depression. Fractalkine (CX3CL1), a chemokine, plays an important role not only in the migration, differentiation and proliferation of neuronal and glial cells but also in the reg...
Article
Full-text available
An increasing number of data support the involvement of disturbances in glucose metabolism in the pathogenesis of depression. We previously reported that glucose and glycogen concentrations in brain structures important for depression are higher in a prenatal stress model of depression when compared with control animals. A marked rise in the concen...
Article
Full-text available
Endocannabinoids (EC), particularly anandamide (AEA), released constitutively in pain pathways might be accountable for the inhibitory effect on nociceptors. Pathogenesis of neuropathic pain may reflect complex remodeling of the dorsal root ganglia (DRGs) and spinal cord EC system. Multiple pathways involved both in the biosynthesis and degradation...
Poster
Neurochemical changes in opioid systems activity in adult mice as consequences of neonatal painful stimulation METHODS Pain experienced in the infancy influence the pain sensitivity in adult life and neuroplasticity induced by neonatal inflammation is the consequence of a combination of activity-dependent changes in neurons. Additionally, the relat...
Poster
There is a strong evidence showing involvement of CB1/TRPV1 receptors system in pathophysiology of neuropathic pain. Our studies focused on the metabolism of anandamide (AEA), an CB1/TRPV1 agonist, which is involved in the development of pain and inflammation. Chronic constriction injury (CCI) of the sciatic nerve was used as a neuropathic pain mod...
Chapter
Full-text available
Oporność wielolekowa uwarunkowana obecnością specyficznych białek transportowych stanowi aktualny i poważny problem we współczesnej farmakologii. Podwyższona ekspresja białek oporności wielolekowej (MDR) w niektórych nowotworach znacząco obniża jakość chemioterapii. W ciągu ostatnich lat zbadano wiele białek odpowiedzialnych za ten proces. Na szcze...
Poster
Chlorophyll derivatives have a well known photosensitive activity. The absorption of red light, a short retention time in the body, an effective generation of reactive oxygen species and the dietary origin render these photosensitisers prominent agents for photodynamic therapy (PDT). Our lead compounds, zinc pheophorbide a and chlorophyllide a, hav...
Poster
Carotenoids are isoprenoid pigments presented in all photosynthetic organisms. Their role as photosystem protectors is regarded as one of the most important and very well known. Different structure of these pigments is thought to have essential impact on their photosynthetic functions, i.e. photoprotection of chlorophylls or light harvesting. The a...

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