Matěj Božik

Matěj Božik
Czech University of Life Sciences Prague | CULS · Department of Food Science

Ph.D.
open to scientific challenges and collaboration

About

32
Publications
5,707
Reads
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359
Citations
Introduction
plant antimicrobials as food preservatives, analytical methods: GC-MS, GC-FID, MALDI-TOF
Additional affiliations
March 2016 - June 2016
Universitat Politècnica de València
Position
  • PhD Student
September 2014 - September 2018
Czech University of Life Sciences Prague
Position
  • PhD Student
September 2014 - present
Czech University of Life Sciences Prague
Position
  • Researcher

Publications

Publications (32)
Article
Full-text available
The antibacterial effects of essential oils and their components (EOCs) are usually attributed to effects on membranes and metabolism. Studies of the effects of EOCs on protein expression have primarily analysed proteins larger than 10 kDa using gel electrophoresis. In the present study, we used MALDI-TOF-MS to investigate the effects of EOCs on lo...
Article
Full-text available
Detection of adulteration of small ruminant milk is very important for health and commercial reasons. New analytical and cost-effective methods need to be developed to detect new adulteration practices. In this work, we aimed to explore the ability of the MALDI-TOF mass spectrometry to detect bovine milk in caprine and ovine milk using samples from...
Article
Cannabis (Cannabis sativa L.) for medical purposes has been legalized again in many countries in recent years. Currently, only two major cannabinoids (Δ⁹-THC and CBD) are considered in the legislation and medication, which is not sufficient in case of dried plant material or resulting extract. Other substances (mainly terpenes/terpenoids), or their...
Article
Full-text available
The main objective of this study is to evaluate the effect of selected essential oils thyme chemotype linalool (Thymus zygis L.), thyme chemotype tymol (Thymus vulgaris L.), eucalyptus (Eucalyptus globulus Labill.), lavender (Lavandula angustifolia Mill.), mint (Mentha piperita L.), almond (Prunbus dulcis Mill.), cinnamon bark (Cinnamomum zeylanicu...
Article
The study aimed to assess the antifungal activity of twenty-five essential oils (EOs) and the potential synergistic activity of the most effective EOs against significant indoor fungi of the genus Aspergillus [A. fumigatus (KBio-122), A. flavus (KBio-134), A. terreus (KBio-145) and A. niger (KBio-202)]. The chemical composition of all EOs was evalu...
Article
Full-text available
The number of described species of the oomycete genus Phytophthora is growing rapidly, highlighting the need for low-cost, rapid tools for species identification. Here, a collection of 24 Phytophthora species (42 samples) from natural as well as anthropogenic habitats were genetically identified using the internal transcribed spacer (ITS) and cytoc...
Article
Full-text available
Essential oil components (EOCs) such as eugenol play a significant role in plant antimicrobial defense. Due to the volatility and general reactivity of these molecules, plants have evolved smart systems for their storage and release, which are key prerequisites for their efficient use. In this study, biomimetic systems for the controlled release of...
Article
The dietary fibre and lipid content of grains is variable. Hence, it is important to estimate the nutritional quality of grains destined for human and animal consumption. We investigated the lipid content and fatty acid composition of new hulled oat cultivars before and after dehulling and naked varieties. The fat content varied from 2.6 g/100 g (y...
Article
Full-text available
Fungal contamination in stored food grains is a global concern and affects food economics and human and animal health. It is clear that there is a need to develop new technologies with improved performances that are also eco-friendly in nature. Due to the bioactivity of essential oils (EOs) in the vapor phase, their low toxicity for humans, and the...
Article
The fat content, fatty acid composition, and atherogenic and thrombogenic indices were determined in five new oat cultivars grown under organic and conventional cropping systems at two experimental localities. The total fat content in the dehulled grain samples ranged from 4.8 to 6.0%. Naked oat Patrik had a significantly higher fat content than th...
Article
As the demand for sustainable, cost-effective food production increases, edible insects are an important alternative to traditional livestock as a source of human food. Understanding the differences in the nutritional composition of edible insects is essential for the optimization of rearing process. This study investigated the effects of developme...
Article
Carvacrol (CAR) is a natural bioactive compound with antioxidant and antimicrobial activity that is present in essential oils. The application of CAR in food preservation is hampered by its high volatility, low solubility in water, and susceptibility to light, heat and oxygen degradation. Polylactide (PLA) is an FDA-approved polymer derived from re...
Article
The Colorado potato beetle ranks as one of the most important potato pests, mainly due to its high feeding rate during all developmental stages, particularly third and fourth larval instar, and high fecundity. The effect of essential oil (EO) from anise (Pimpinella anisum L. [Apiales: Apiaceae]) prepared as conventional and encapsulated (EN) formul...
Article
The major objective of this study was to determine the antifungal, anti-toxicogenic and anti-sporulation effect of selected essential oils in the control growth of Aspergillus spp. by contact vapour method in in vitro and in situ condition. In continue, the effect of the essential oils treatment on the sensory profile of the bread was evaluated. In...
Article
The objective of this study was to evaluate in vitro and in vivo potential of a specific commercial blend of essential oil active compounds (BEO) to improve rumen fermentation and general dairy cow performance. First, the main active components of the BEO were identified and quantified. Then, the in vitro experiment was conducted using 24 h batch i...
Article
Essential oil components (EOCs) are potent natural antimicrobial agents, but their high volatility and reactivity limit their potential applications. Here, we report the preparation of a series of nanomaterials containing EOCs (i.e., allyl isothiocyanate, carvacrol, cinnamaldehyde, diallyl disulphide, eugenol and thymol). The materials were prepare...
Article
Full-text available
In the present study, the antimicrobial activity of sage oil, some of its pure components (1,8-cineole, borneol and α-β thujone) and sage extract were investigated against selected strains of Gram-positive bacteria (Enterococcus faecalis, Staphylococcus aureus) and Gram-negative bacteria (Klebsiella pneumoniae, Escherichia coli). The antimicrobial...
Article
Full-text available
Bread is considered an intermediate - moisture food product that is prone to mould spoilage. Growth of spoilage fungi is currently controlled with the addition of chemical preservatives. Consumers demand more natural products and there is a need to reduce the amount of chemical preservatives added to foods. Essential oils (EOs) and their constituen...
Article
Full-text available
In this dataset we provide MALDI-TOF MS spectra of Escherichia coli. The data presented in this article are related to the article entitled “Stress response of Escherichia coli to essential oil components – insights on low-molecular-weight proteins from MALDI-TOF” (Božik et al., 2018) [1]. Essential oils and their components are known for their ant...
Article
Oat is a cereal with a long history of cultivation and great importance in human nutrition, not only because of its starch and protein content, but also because of its substantial fat content with a relatively high proportion of unsaturated fatty acids. The fat content, fatty acid composition, and nutritional value of six new oat cultivars were eva...
Article
Since January 2018, insects have been recognised as novel foods in the EU, but their nutritional value varies, and factors affecting their nutritional composition have been debated. We investigated the effect of sex on the nutritional value and chemical composition of the house cricket (Acheta domestica L.). Both sexes were rich in protein and lipi...
Article
The main objective of this study was using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for assembling of DSM (German Collection of Microorganisms) Streptomyces spectral database and identification of wild Streptomyces cultures, which were clustered by MALDI-TOF Biotyper OC software as well as for tera...
Article
Full-text available
Essential oils play a prominent role as flavouring agents and fragrances in the food and perfume industries. Carvacrol is a major component of various essential oils, such as oregano and thyme oils, and is responsible for their antimicrobial activity. Lauric acid is a medium-chain fatty acid (MCFA) with a high antibacterial potential. Both carvacro...
Article
In the last decades, there has been a significant increase in the number of lifestyle and auto-immune diseases, such as various cancers or multiple sclerosis. In countries where cannabis is decriminalized for medical purposes, it is most often prescribed for these diagnoses. Today, over 700 different cannabis genotypes are being bred, and it is ver...
Article
Purpose: Plant-derived essential oils and their encapsulations have recently received increasing interest as an alternative to synthetic biopesticides suitable for integrated pest management and organic farming. In this study, combinations of essential oils (EO) from Rosmarinus officinalis with Cymbopogon citratus and Pelargonium graveolens with Th...
Article
Full-text available
Essential oils are volatile substances from plants and many of them have antimicrobial activity. For that reason, they have become known as a useful alternative to chemical preservatives and pesticides. In this study, we tested essential oils of four aromatic plants. Cinnamon (Cinnamomum zeylanicum), thyme (Thymus vulgaris), oregano (Origanum vulga...
Article
Full-text available
Antimicrobial volatile substances from plants represent alternatives to synthetic pesticides and food preservatives. In this study, the compositions of some essential oils were determined by gas chromatography with mass spectrometry, and the inhibitory properties of the essential oils and their components against the bacterial postharvest pathogens...
Article
The effect of essential oil (EO) from anise (Pimpinellia anisum) on the mortality of young larvae of Colorado potato beetles has been studied. In our bioassays, P. anisum EO significantly increased the mortality of the second instar larvae of L. decemlineata. Significantly different values of LD50 and LD90 were established for acute (LD50 = 1.76, a...
Article
Traditionally, chemical pesticides have played a central role in food protection, forcing trends towards reduction of using chemicals in agriculture leads to call for the development of new strategies of protection agricultural product. Antimicrobial volatile substances from plants have become known as a suitable alternative to synthetic pesticides...
Article
The bacterial diversity of Pectinatella magnifica colonies sampled from pounds in South Bohemia during the summer of 2012 was investigated. The bacterial counts determined after cultivation on modified yeast extract-tryptone agar (Oxoid) supplemented with glucose (1 g L-1) varied from 4.22 to 6.61 and from 1.30 to 6.85 log CFU/g for aerobes and ana...
Article
Fecal bacteria from 33 infants (aged 1 to 6 months) were tested for growth on commercial prebiotics. The children were born vaginally (20) or by caesarean section (13). Bifidobacteria, lactobacilli, gram-negative bacteria, Escherichia coli, and total anaerobes in fecal samples were enumerated by selective agars and fluorescence in situ hybridizatio...

Questions

Questions (3)
Question
My windows 7 / HDD failed during the update. The system does not boot, but the disk remains readable. Do you know some hack how to get the Biotyper database from disk? I received an answer from Bruker's support that the backup can only be created from the running Biotyper. Unfortunately, I have never made any backups, and I have many of my own strains and species in my database. I still believe in some hack how to do it.
Question
Hi,
I am currently looking for a quick and simple method for staining Pectobacterium in microtiter plate without any prior treatment or minimal treatment.
I have tried MTT, it worked perfectly with the Bacillus but did not work with Pectobacterium. It is fast and very simple to prepare and evaluate.
Some recommendations why it does not work with Pectobacterium?
What can I use instead MTT? I would like preform test directly in broth in microtiter plate. I will use it for determination of minimum inhibitory concentration, so I need to detect only live cells.
Thank you for your suggestions.
Question
I am looking for anyone with experience with statistical comparison of MALDI-TOF data. I have no experience with analyzing profile data and I need to compare the effect of the treatment on bacterial culture. It should work in MathLab or Bionumerics.

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Projects

Projects (4)
Project
Infrastructure for Promoting Metrology in Food and Nutrition High-level metrology services in food and nutrition for the enhancement of food quality and safety METROFOOD-CZ is Czech national node of METROFOOD-RI METROFOOD-CZ will provide services to different categories of users: researchers / academics, food control agencies and inspectors, food merchants, consumers and the general public.
Project
The aim of this project is to assess yield and quality of edible fungi from different growing conditions. To investigate genetic and protein/peptide variability of wild and cultivated species of edible and non-edible fungi. From this information, to create complementary datasets and methods used to fungal strains identification by genetic method DNA barcoding analysis and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) biotyping.
Project
The aim of this project is to investigate the proteome of E. coli. exposed to natural antimicrobials, which are compounds of essential oils.