Masrina Mohd Nadzir

• PhD in Engineering, Osaka University
• Associate Professor at University of Science Malaysia

Curcumin is widely used as a therapeutic drug for cancer treatment. However, its limited absorption and rapid excretion are the major therapeutic limitations to its clinical use. Using niosomes as a curcumin delivery system is a cheap, easy, and less toxic strategy for enhancing the absorption of curcumin by cells and delaying its excretion. Thus,...

Asiaticoside is one of the biomarker components in Centella asiatica that possesses extraordinary medicinal properties. It can be used as a source of natural ingredients in the pharmaceutical and cosmeceutical industries. In this work, asiaticoside was extracted using ultrasound and betaine-based natural deep eutectic solvent (NADES). The kinetics...

Cinnamomum cassia is a tropical medicinal and aromatic plant from the Lauraceae family, which is commonly used as a natural spice in food preparation and traditional medicine to treat respiratory and digestive disorders. The extract of C. cassia contains bioactive compounds, such as cinnamaldehyde, cinnamic alcohol, cinnamic acid, and cinnamate. Re...

Infections by ESKAPE (Enterococcus sp., Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp.) pathogens cause major concern due to their multi-drug resistance (MDR). The ESKAPE pathogens are frequently linked to greater mortality, diseases, and economic burden in healthcare worldwide....

Centella asiatica is known for its valuable medicinal properties which highlight its potential application in the pharmaceutical industry. In this work, the ultrasound-assisted extraction (UAE) of asiaticoside from C. asiatica using betaine-based natural deep eutectic solvents (NADES) was developed for the first time. Eleven different NADES were sc...

Solvent-free microwave extraction (SFME) is a green extraction method that requires no solvent and has a short extraction time. This study extracted Centella asiatica using SFME and analysed the extract for its phytoconstituents, phenolic content, antioxidant, and antimicrobial activity. Extraction of 20 g fresh C. asiatica by SFME was carried out...

Eleven different types of natural deep eutectic solvent (NADES) were synthesised using betaine (Bet) as a hydrogen bond acceptor (HBA) with glycerol (Gly), 1,2-propanediol (1,2P), lactic acid (LA), levulinic acid (Lev), anhydrous citric acid (CA), malic acid (MA), tartaric acid (TA), xylitol (Xyl), sorbitol (Sor), glucose (Glu), and sucrose (Suc) a...

Anthocyanins are natural water-soluble colourants with a number of reported health benefits and are an excellent alternative to artificial dyes. Anthocyanins from Clitoria ternatea were obtained using ultrasound-assisted extraction with glycerol-water (60:40 w/v). The anthocyanins in the extract were measured at wavelength 550 nm and 700 nm using a...

Microbes (e.g., bacteria, fungi, yeast, and algae) are beneficial in our daily life. Utilization of microbes in the preparation of food products like cheese, yoghurt, and bread is well known. Still, in reality, there are many other uses of microbes that contribute massively in our life, such as the production of cleaning products, cosmetics, and te...

The principle of green chemistry is crucial in the industrial processing of natural products. In particular, the extraction process of bioactive compounds from plant sources which involve food, nutraceutical, pharmaceutical, and cosmetic industries demand the use of green solvents. This is important to produce a value-added product that is safe for...

The interest of consumers in using products containing phytochemicals derived from plants is growing day by day due to the shift of consumers’ preferences from convenience to environmental sustainability. One plant utilized in many products is Centella asiatica, a herb commonly used in folk medicine, health supplements, and beauty products. Extract...

Bacterial exopolysaccharides (EPSs) are an essential group of compounds secreted by bacteria. These versatile EPSs are utilized individually or in combination with different materials for a broad range of biomedical field functions. The various applications can be explained by the vast number of derivatives with useful properties that can be contro...

The current strategy for rapid wound healing treatment involves combining a biomaterial and cell-secreted proteins or biomolecules. This study was aimed at characterizing 3-dimensional (3D) collagen hydrogels fortified with dermal fibroblast-conditioned medium (DFCM) as a readily available acellular skin substitute. Confluent fibroblasts were cultu...

Green synthesis of particles involves the use of safe biological agents as an eco-friendly and cost-effective alternative to chemical synthesis. In this study, silver particles were biosynthesised by using silver nitrate and aqueous Citrus microcarpa peel extract as the reducing and stabilising agent. The synthesised silver particles were confirmed...

Centella asiatica (C. asiatica) is a common herb found in the Indian subcontinent, Southeast Asia, and wetland regions of the Southern US. Although it grows abundantly in these regions, very few reports are available on the modern extraction of C. asiatica, especially by the solvent-free microwave extraction (SFME) method. In this study, the proces...

Alpinia galanga (A. galanga) is a popular culinary ingredient as well as medicinal herb commonly found in Southeast Asia. Compared to the rhizome of A. galanga, the leaves and stems are always being discarded as waste. In this research, active compounds from the stem and leaves of this plant were extracted using maceration technique with ethanol as...

Normally discarded as waste, ovine tendon collagen has great potential for use as an injectable hydrogel. Typical collagen used for biomedical applications is derived from epidermal tissue. Compared to the epidermal collagen, tendon collagen consists of collagen fibrils with large diameters. This limits the conjugation of phenolic hydroxyl (Ph) gro...

Silver nanoparticles (AgNPs) were produced utilizing the aqueous extract of Colubrina asiatica (C. asiatica) and AgNO 3 solution. The absorption spectra, morphology and size were analyzed using UV-vis spectroscopy, transmission electron microscopy and dynamic light scattering method, respectively. The effect of various parameters i.e., reaction tim...

Nanoparticle is increasing in demand especially in the area such as cosmetics, biomedical sciences, environmental health and drug delivery which mainly due to its minute particle size. Gynura procumbens (G. procumbens) is a medicinal plant that easily found in Southeast Asia and can be used to synthesize silver nanoparticles (AgNPs) without any har...

Silver nanoparticles (AgNPs) were produced utilizing the aqueous extract of Colubrina asiatica (C. asiatica) and AgNO3 solution. The absorption spectra, morphology and size were analyzed using UV-vis spectroscopy, transmission electron microscopy and dynamic light scattering method, respectively. The effect of various parameters i.e., reaction time...

In this research, injectable collagen-chitosan conjugates (Col-Chit-Ph) hydrogel was formed by oxidative coupling of phenolic hydroxyl (Ph) groups in polymer chain via peroxidase-catalysed crosslinking reaction. The resulting Col-Chit-Ph solutions formed rapid hydrogel at physiological conditions by utilizing H2O2. The amount of chitosan in the com...

The objective of this study was to evaluate the effects of addition of membrane additives, cholesterol (chol) with further molecular charges modification using Dicetylphosphate (DCP) and Stearylamine (SA) on stability of curcumin niosomes. Niosomes with entrapped curcumin were prepared by thin-film hydration method. This vesicular drug carrier was...

In this research, the fabrication of collagen/chondroitin 4-sulphate (C4S) hydrogel scaffold for biomedical applications was studied. The influence of C4S content on physical, mechanical, and biological properties of scaffold were investigated. It was found that high C4S content significantly increased the swelling capacity of scaffold. Furthermore...

In this research, the fabrication of collagen/chondroitin 4-sulphate (C4S) hydrogel scaffold for biomedical applications was studied. The influence of C4S content on physical, mechanical, and biological properties of scaffold were investigated. It was found that high C4S content significantly increased the swelling capacity of scaffold. Furthermore...

Introduction: Collagen type I is widely used as a biomaterial for tissue-engineered substitutes. This study aimed to fabricate different three-dimensional (3D) scaffolds using ovine tendon collagen type I (OTC-I), and compare the attachment, proliferation and morphological features of human dermal fibroblasts (HDF) on the scaffolds. Methods: Th...

Introduction: Collagen type I is widely used as a biomaterial for tissue-engineered substitutes. This study aimed to fabricate different three-dimensional (3D) scaffolds using ovine tendon collagen type I (OTC-I), and compare the attachment, proliferation and morphological features of human dermal fibroblasts (HDF) on the scaffolds. Methods: Thi...

Centella asiatica (C. asiatica) or known as pegaga in Malaysia is commonly eaten raw by local as it is claimed to have medicinal purposes such as for healing of wound, and kidney diseases. Furthermore, this plant is said to have antimicrobial properties. Since extraction is an important step for separation of bioactive compounds from the plant mate...

The effect of insulin-like growth factor-1 (IGF-1) on the behavior of rabbit chondrocytes in cultured collagen (CL) gels initially seeded with 2 × 10(5) cells/ml was examined. On day 5, the frequency of migrating cells cultured in presence of 100 ng IGF-1/ml was 0.04, which was 54 % of the frequency in IGF-1-free culture. The presence of IGF-1 caus...

The degree of collagen fibril formation was altered by varying the preservation conditions. The collagen substrate under atmosphere of nitrogen gas for 4 days exhibited well-developed collagen fibril network accompanied with the frequency of round-shaped chondrocyte cells (f(R)) of 0.62, the value of which was slightly lower than that on the refere...

A high density collagen type I coated substrate (CL substrate) was used to evaluate the chondrocyte phenotypes in passaged cultures. With increasing age of cell population (population doubling (PD)=0-14.5), the frequency of non-dividing spindle shaped cells without ALP activity increased, accompanied with an increase in gene expression of collagen...

In static culture of rabbit chondrocytes in collagen gel, the direct measurement of dissolved oxygen (DO) concentration revealed that the DO level at the top surface of gel decreased due to an increase in overall cell density with elapsed time. The local cell density at the top surface on day 21 was 5.7x10(7) cells/cm(3), being 11 times that at the...

A laboratory-scale study was conducted to evaluate the feasibility of using palm oil mill effluent (POME) as a major substrate and other nutrients for maximum production of citric acid using the potential fungal strain Aspergillus niger (A103). Statistical optimization of medium composition (substrate–POME, co-substrates–wheat flour and glucose, an...

In this study, screening of potential microbes, especially Aspergillus, for citric acid production from Palm Oil Mill Effluent (POME) is carried out to improve the product yield. The fermentation of the raw material POME for the production of citric acid was conducted by the liquid state fermentation process. A total of ten strains of Aspergillus w...