Masayoshi Tanaka

Masayoshi Tanaka
Tokyo Institute of Technology | TITech · Department of Chemical Science and Engineering

PhD

About

99
Publications
10,937
Reads
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2,413
Citations
Additional affiliations
April 2016 - December 2016
Tokyo Institute of Technology
Position
  • Professor (Assistant)
September 2011 - March 2014
Tokyo University of Agriculture and Technology
Position
  • Professor (Assistant)
September 2009 - August 2011
University of Leeds
Position
  • PDRA

Publications

Publications (99)
Article
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Tumors grow by receiving oxygen and nutrients from the surrounding blood vessels, leading to rapid angiogenesis. This results in functionally and structurally abnormal vasculature characterized by high permeability and irregular blood flow, causing hypoxia within the tumor microenvironment (TME). Hypoxia exacerbates the secretion of pro-angiogenic...
Article
Biological membranes, composed of lipids and proteins, exhibit dynamic curvature that influences cellular processes such as division, transport, and signaling. Curvature–sensing proteins, like BAR domain proteins, are known in eukaryotes for recognizing membrane shapes and inducing structural changes. Recent studies show that prokaryotes also posse...
Article
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While the utility of supported metal and alloy clusters as catalytic materials is widely recognized, their precise synthesis remains a challenge. Here, we demonstrate the precise synthesis of these clusters via metallopeptides. This technique is characterized by its ability to be automated using Merrifield's solid-phase peptide synthesis (SPPS). Me...
Article
Replicating the sense of smell presents an ongoing challenge in the development of biomimetic devices. Olfactory receptors exhibit remarkable discriminatory abilities, including the enantioselective detection of individual odorant molecules. Graphene has emerged as a promising material for biomimetic electronic devices due to its unique electrical...
Article
Migrasomes are extracellular vesicles that form on the retraction fibers of migrating cells. In this study, we report the formation of migrasome‐like vesicles enriched in tetraspanin 4 and containing cytoplasmic components in response to hypoosmotic stress. When migrating cells were subjected to hypoosmotic stress, vesicles with a size distribution...
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Infectious pathogens can be transmitted through textiles. Therefore, additional efforts are needed to develop functional fabrics containing antimicrobial substances to prevent the growth of antibiotic-resistant bacteria and their biofilms. Here, we developed a cotton fabric coated with reduced graphene oxide (rGO) and copper nanoparticles (Cu NPs),...
Article
Methylation of cytosine to 5-methylcytosine on CpG dinucleotides is the most frequently studied epigenetic modification involved in the regulation of gene expression. In normal tissues, tissue-specific CpG methylation patterns are established during development. In contrast, alterations in methylation patterns have been observed in abnormal cells,...
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CD81, a transmembrane protein belonging to the tetraspanin family, has recently been suggested as a therapeutic target for cancers. Here, we screened peptides that bind to the tetraspanin CD81 protein, and evaluated their inhibitory activity in cancer cell migration. To screen for CD81-binding peptides (CD81-BP), a peptide array membrane was prepar...
Article
As an organizer of multi-molecular membrane complexes, the tetraspanin CD9 has been implicated in a number of biological processes, including cancer metastasis, and is a candidate therapeutic target. Here, we evaluated the suppressive effects of an eight-mer CD9-binding peptide (CD9-BP) on cancer cell metastasis and its mechanisms of action. CD9-BP...
Article
An olfactory receptor mimetic peptide-modified graphene field-effect transistor (gFET) is a promising solution to overcome the principal challenge of low specificity graphene-based sensors for volatile organic compound (VOC) sensing. Herein, peptides mimicking a fruit fly olfactory receptor, OR19a, were designed by a high-throughput analysis method...
Article
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Bioactive molecules and their effects have been influenced by their solubility and administration route. In many therapeutic reagents, the performance of therapeutics is dependent on physiological barriers in the human body and delivery efficacy. Therefore, an effective and stable therapeutic delivery promotes pharmaceutical advancement and suitabl...
Article
A cytopathology test for cervical cancer diagnosis has been introduced to complement the histopathology test. This study developed a new cervical cancer diagnostic method that complements the existing liquid cytology to reduce the diagnostician’s skill dependence and to improve diagnostic accuracy. With the ease of introduction of surface functiona...
Article
For the past 200 years, lactate has been regarded as a metabolic waste end product that causes fatigue during exercise. However, lactate production is closely correlated with energy metabolism. The lactate dehydrogenase-catalyzed reaction uses protons to produce lactate, which delays ongoing metabolic acidosis. Of note, lactate production differs d...
Article
Gas sensing based on graphene field-effect transistors (GFETs) has gained broad interest due to their high sensitivity. Further progress in gas sensing with GFETs requires to detection of various odor molecules for applications in the environmental monitoring, healthcare, food, and cosmetic industries. To develop the ubiquitous odor-sensing system,...
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A new route to single‐step and non‐covalent immobilization of proteins on graphene is exemplified with the first biosensor for nitriles based on a graphene field‐effect transistor (GFET). The biological recognition element is a fusion protein consisting of nitrile reductase QueF from Escherichia coli with an N‐terminal self‐assembling and graphene‐...
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Owing to increased environmental pollution, active research regarding microplastics circulating in the ocean has attracted significant interest in recent times. Microplastics accumulate in the bodies of living organisms and adversely affect them. In this study, a new method for the rapid detection of microplastics using peptides was proposed. Among...
Article
Cancer immunotherapy is an emerging therapeutic strategy for cancer treatment. Most of the immunotherapeutics approved by the FDA regulate the innate immune system and associated immune cell activity, with immune check inhibitors in particular having transformed the field of cancer immunotherapy due to their significant clinical potential. However,...
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Microplastics are found in various environments with the increasing use of plastics worldwide. Several methods have been developed for the sampling, extraction, purification, identification, and quantification of microplastics in complex environmental matrices. This study intends to summarize recent research trends on the subject. Large microplasti...
Article
Bacteria display dynamically organized curved membrane structures, especially during cell division. The importance of membrane curvature-sensing (MCS) proteins for the recognition and regulation of biological membrane morphologies has predominately been investigated in eukaryotic cells. Recently, a technique for screening MCS proteins from solution...
Article
Extracellular vesicles (EVs) are cell-to-cell communication tools. Migrasomes are recently discovered microscale EVs formed at the rear ends of migrating cells, and thus are suggested to be involved in communicating with neighboring cells. In cell culture, peptide scaffolds on substrates have been used to demonstrate cellular function for regenerat...
Article
Pulmonary tuberculosis is a highly prevalent respiratory disease that affects approximately a quarter of the world's population. The drug treatment protocol for tuberculosis is complex because the Mycobacterium tuberculosis (M. tuberculosis) invades macrophages and begins to infect. Thus treatment usually includes combination therapy with several d...
Article
Hypoxic microenvironments emerge as tumor grow, leading to the over-expression and stabilization of hypoxia-inducible factor 1-alpha (HIF-1α). HIF-1α lowers the sensitization against chemotherapy, radiation therapy and photodynamic therapy in cancer. In this study, nano-sized oxygen carrier, namely oxygen dissolved nanoliposome (ODL) was synthesize...
Article
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Triangular Au nanoplates (TrAuNPls) possessing strong plasmonic properties can be used as photothermal agents in cancer therapy. However, the controlled preparation of such morphologies typically requires harsh synthetic conditions. Biomolecules offer an alternative route to developing biocompatible synthetic protocols. In particular, peptides offe...
Article
A CD9-binding peptide (RSHRLRLH), screened from EWI-2, was characterized, and its inhibition effect on cancer-cell migration and invasion was demonstrated.
Article
Designing cell-electrode biointerfaces is important for electrochemical monitoring of cell functions and activities in their microenvironments. Herein, peptide monolayer was constructed on the pyrolytic graphite surface by self-assembling of tritryptophane, and the bi-functionalized peptide conjugating cell adhesion peptide, GRGDS (Gly-Arg-Gly-Asp-...
Article
Cancer-derived circulating exosomes or nanoscale extracellular vesicles are emerging biomarkers for disease detection and treatment because of their cell-specific constituents and unique intercellular pathways. For efficient exosome isolation from bio-fluids, the design of high-affinity nanointerfaces is of great importance in the development of mi...
Article
Natural polymer materials have high biocompatibility and are not toxic because they can decompose into metabolites in the body; thus, they are widely used as medical polymer materials as well as porous support materials. As a biopolymer, alginate has a negative charge; in contrast, chitosan has a positive charge. Therefore, when the two substances...
Article
Membrane curvature-sensing (MCS) proteins recognize and regulate the morphologies of biological membranes. As these proteins lack characteristic sequence motifs in their primary structure, they are not instantly recognizable by genomic databases. Overcoming this technological challenge toward the agile identification of new proteins can promote the...
Article
Some peptides have been utilized in the functionalization of nanomaterials, the control of nanoparticle (NP) synthesis, as biotemplating for morphological control, and biocatalysts for the NP crystallization. The catalytic peptides used for NP synthesis, named biomineralisation peptides, generally demonstrate high specific binding to a target mater...
Article
The complete recovery of tissues or cells damaged by an accident or illness is a major issue in medicine. With increasing medical costs and incidences of incurable diseases, this aspect is emerging as an important human health problem worldwide. However, reproducing actual cells and tissues is not easy because of the involvement of several variable...
Article
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A rapid method for screening pathogens can revolutionize health care by enabling infection control through medication before symptom. Here we report on label-free single-cell identifications of clinically-important pathogenic bacteria by using a polymer-integrated low thickness-to-diameter aspect ratio pore and machine learning-driven resistive pul...
Article
Integration of a large‐sized DNA fragment into a chromosome is an important strategy for characterization of cellular functions in microorganisms. Magnetotactic bacteria synthesize intracellular organelles comprised of membrane‐bound single crystalline magnetite, also referred to as magnetosomes. Magnetosomes have gained interest in both scientific...
Article
The topical delivery of siRNA‐based therapies has opened new avenues for the treatment of skin disorders. The use of siRNA as a therapeutic, however, is limited due to its rapid degradation and poor cellular uptake. Furthermore, the top layer of skin, the stratum corneum, is a major barrier to the delivery of topical agents. There is an unmet need...
Article
Using protein and peptide additives to direct the crystallisation of inorganic materials is a very attractive and environ-mentally friendly strategy to access complex and sometimes inaccessible mineral phases. CoPt is a very desirable high-magnetoanisotropic material in its L10 phase, but this is acquired by annealing at high temperatures which is...
Article
With increased awareness among consumers regarding food safety and security, food allergen control has become an indispensable requirement in the food industry. Although several methods for detecting allergens in food products are available, highly sensitive techniques are required. In this study, we developed a technique named as peptide array-bas...
Article
Explosive compounds, such as 2,4,6-trinitrotoluene (TNT), pose a great concern in terms of both global public security and environmental protection. There are estimated to be hundreds of TNT contaminated sites all over the world, which will affect the health of humans, wildlife, and the ecosystem. Clearly, the ability to detect TNT in soils, water...
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The use of biomolecules in nanomaterial synthesis has received increasing attention, because they can function as a medium to produce inorganic materials in ambient conditions. Short peptides are putative ligands that interact with metallic surfaces, as they have the potential to control the synthesis of nanoscale materials. Silver nanoparticle (Ag...
Article
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Quantum dots (QDs) are promising nanomaterials due to their unique photophysical properties. For them to be useful in biological applications, the particle surface generally needs to be conjugated to biological molecules, such as antibodies. In this study, we screened CdTe/CdS QD-binding peptides from a phage display library as linkers for simple a...
Article
A novel peptide-based three-dimensional probe called "peptide matrix," inspired by the antibody paratope region, was fabricated on a surface plasmon resonance (SPR) sensor chip to enhance the sensitivity of detecting the explosive 2,4,6-trinitrotoluene (TNT). Although peptide aptamer is an attractive candidate for a molecular recognition probe beca...
Article
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Due to unique properties arising from its 2D nature, molybdenum disulfide (MoS2) has been studied widely toward its application for biosensing. While MoS2 field effect transistor has been utilized for electrical detection of biological events in many studies, photoluminescence (PL) characteristics of MoS2 have been poorly employed. MoS2 PL can prov...
Article
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Diseases caused by antibiotic-resistant bacteria are challenging to treat, and biofilms, which are polymeric conglomerations of bacterial extracellular polysaccharides, proteins, lipids, and DNA, form rigid barriers that prevent external antibacterial reagents from penetrating. In synergy, antibiotic-resistant bacteria and biofilms are highly resis...
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Biomineralisation peptides that facilitate the one-pot synthesis of gold nanoparticles (AuNPs) with selected optical properties, were screened using a coherent peptide-spotted array consisting of a AuNP binding peptide library.
Article
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Nanodiamonds are emerging as new nanoscale materials because of their chemical stability, excellent crystallinity, and unique optical properties. In this study, the structure of nanodiamonds was engineered to produce carbon nano-onion particles (CNOs) with multiple layers. Following a series of physicochemical characterizations of the CNOs, various...
Article
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In this study, we developed a surface plasmon resonance (SPR) sensor chip based on 2,4,6-trinitrotoluene (TNT) recognition peptide-modified single-walled carbon nanotubes (SWCNTs). The carboxylic acid-functionalized SWCNTs were immobilized on a 3-aminopropyltriethoxysilane (APTES)-modified SPR Au chip surface. Through π-stacking between the aromati...
Article
Immunosensing is a bioanalytical technique capable of selective detections of pathogens by utilizing highly specific and strong intermolecular interactions between recognition probes and antigens. Here, we exploited the molecular mechanism in artificial nanopores for selective single-virus identifications. We designed hemagglutinin antibody mimicki...
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Abstract Rapid diagnosis of flu before symptom onsets can revolutionize our health through diminishing a risk for serious complication as well as preventing infectious disease outbreak. Sensor sensitivity and selectivity are key to accomplish this goal as the number of virus is quite small at the early stage of infection. Here we report on label-fr...
Article
Lipid tubules are of particular interest for many potential applications in nanotechnology. Among various lipid tubule fabrication techniques, the morphological regulation of membrane structure by proteins mimicking biological processes may provide the chances to form lipid tubes with highly‐tuned structures. Magnetotactic bacteria synthesize magne...
Chapter
Magnetotactic bacteria are a group of microorganism producing nano-sized magnetic particles. The bacterial cells accumulate a large amount of iron ion from aquatic environment and synthesize single-crystal magnetic nano-particles under ambient conditions. The size, shape, and composition of the magnetic nano-particles are precisely regulated in ind...
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Short functional peptides are promising materials for use as targeting recognition probes. Toll-like receptor 4 (TLR4) plays an essential role in pathogen recognition and in activation of innate immunity. Here, the TLR4 amino acid sequence was used to screen for bacterial cell binding peptides using a peptide array. Several octamer peptides, includ...
Article
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Particulate matter (PM) is becoming an increasing health concern and there is a need to develop detection methods to keep its harmful effects in check. Generation of reactive oxygen species (ROS) by PM is often associated with metal compounds, hence our aim is to screen for a peptide probe towards improved collection and the detection of PM having...
Article
In this study, 2,4,6-trinitrotoluene (TNT) binding peptide was synthesized and screened for TNT specific detection using surface plasmon resonance (SPR) sensor. The TNT binding peptide was rational design and synthesized through amino acid sequence from complementarity determining region (CDR) in the anti-TNT monoclonal antibody, which was produced...
Article
Bioinspired pore sensing for selective detection of flagellated bacteria was investigated. The Au micropore wall surface was modified with a synthetic peptide designed from toll-like receptor 5 (TLR5) to mimic the pathogen-recognition capability. We found that intermolecular interactions between the TLR5-derived recognition peptides and flagella in...
Article
Probiotics are viable microbes in food and feed supplements that are beneficial to the health of humans and animals. A direct peptide–bacteria binding assay for identification of Toll-like receptor 2 (TLR2)-derived peptides binding to a probiotic bacterium, Lactobacillus rhamnosus GG, was performed using an octameric-peptide library spot-synthesize...
Article
Full-text available
In this study, a rationally-designed 2,4,6-trinitrotoluene (TNT) binding peptide derived from an amino acid sequence of the complementarity-determining region (CDR) of an anti-TNT monoclonal antibody was used for TNT detection based on a maleimide-functionalized surface plasmon resonance (SPR) sensor. By antigen-docking simulation and screening, th...
Article
Complementarity-determining regions (CDRs) are sites on the variable chains of antibodies responsible for binding to specific antigens. In this study, a short peptide probe for recognition of 2,4,6-trinitrotoluene (TNT), was identified by testing sequences derived from the CDRs of an anti-TNT monoclonal antibody. The major TNT-binding site in this...
Article
Particulate matter (PM), especially particulates with diameters of less than 2.5 μm, can penetrate the alveolar region and increase the risk of respiratory diseases. This has stimulated research efforts to develop detection methods so that counter measures can be taken. In this study, four PM binding peptides were obtained by phage display and bind...
Article
Statement of significance: The development of a technique for functionally regulated nanosized material production in ambient condition is broadly required according to the expansion of nanomaterial based applications. Short peptides, which bind to metallic surfaces, have great potential for the technique development, but the realization remains a...
Article
An on-chip gene expression analysis compartmentalized in droplets was developed for detection of cancer cells at a single-cell level. The chip consists of a keyhole-shaped reaction chamber with hydrophobic modification employing a magnetic bead-droplet-handling system with a gate for bead separation. Using three kinds of water-based droplets in oil...
Article
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Living organisms produce finely tuned biomineral architectures with the aid of biomineral-associated proteins. The functional amino acid residues in these proteins have been previously identified using in vitro and in silico experimentation in different biomineralization systems. However, the investigation in living organisms is limited owing to th...
Article
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Importance: Magnetotactic bacteria synthesize magnetite (Fe3O4) nanocrystals in a prokaryotic organelle called the magnetosome. This organelle is formed using various magnetosome proteins in multi-steps including vesicle formation, magnetosome alignment, and magnetite crystal formation to provide compartmentalized nanospaces for the regulation of...
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Living organisms can produce inorganic materials with unique structure and properties. The biomineralization process is of great interest as it forms a source of inspiration for the development of methods for production of diverse inorganic materials under mild conditions. Nonetheless, regulation of biomineralization is still a challenging task. Ma...
Article
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Importance: The development of a technique for effective environmental water remediation is urgently required across the globe. A biological remediation process of waste removal and/or neutralization of pollutant from contaminated water using microorganism has great potential, but cell recovery remains a bottleneck. Magnetotactic bacteria synthesi...
Article
Simple and rapid tools for antibody detection are beneficial for therapeutic monoclonal antibody development. Using a synthetic cationic antibody-recognizing peptide, a label-free colorimetric assay for antibody detection was established, in which a solution containing silver nanoparticles (AgNPs) changes color depending on particle aggregation/dis...
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Microalgal oil is a promising alternative feedstock for biodiesel fuel (BDF). Mixotrophic cultivation with glycerol, the primary byproduct of BDF production, may be used to optimize BDF production. This strategy would reduce costs through glycerol recycling and improve lipid productivity and biomass productivity by overcoming the growth retardation...
Article
A molecular peptide beacon was designed for fluorescence detection of IgG in a homogeneous assay. pH-triggered detection of IgG was demonstrated using a fluorophore-labeled peptide that incorporated a binding site in the Fc region of IgG with a complementary quenching site.
Article
Rapid and sensitive detection of microorganisms is important in cosmetics, foods, and pharmacy manufacturing from the aspect of biocontrol. The PCR test could be a potential alternative technology enabling rapid detection. However, it also meets technical difficulties that genome of microorganisms should be amplified from extremely low DNA concentr...
Article
Label-free colorimetric assays using metallic nanoparticles have received much recent attention, for their application in simple and sensitive methods for detection of biomolecules. Short peptide probes that can bind to analyte biomolecules are attractive ligands in molecular nanotechnology; however, identification of biological recognition motifs...