Masatoshi Morimatsu

Masatoshi Morimatsu
Okayama University · Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences

Doctor of Philosophy

About

50
Publications
2,833
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
607
Citations
Additional affiliations
February 2012 - July 2015
Stanford University
Position
  • PostDoc Position
April 2010 - February 2012
Osaka University
Position
  • PostDoc Position

Publications

Publications (50)
Article
Cardiomyocytes are contractile cells that regulate heart contraction. Ca²⁺ flux via Ca²⁺ channels activates actomyosin interactions, leading to cardiomyocyte contraction, which is modulated by physical factors (e.g., stretch, shear stress, and hydrostatic pressure). We evaluated the mechanism triggering slow contractions using a high-pressure micro...
Article
Full-text available
Stem cell-based therapy has been used to treat ischaemic heart diseases for two decades. However, optimal cell types and transplantation methods remain unclear. This study evaluated the therapeutic effects of human umbilical cord mesenchymal stem cell (hUCMSC) sheet on myocardial infarction (MI). Methods hUCMSCs expressing luciferase were generate...
Article
Full-text available
Rab11b, abundantly enriched in endocytic recycling compartments, is required for the establishment of the machinery of vesicle trafficking. Yet, no report has so far characterized the biological function of Rab11b in osteoclastogenesis. Using in vitro model of osteoclasts differentiated from murine macrophages like RAW-D cells or bone marrow-derive...
Article
Mechanical stimulation is well known to be important for maintaining tissue and organ homeostasis. Here, we found that hydrostatic pressure induced nuclear translocation of a forkhead box O (FOXO) transcription factor DAF-16, in C. elegans within minutes, whereas the removal of this pressure resulted in immediate export of DAF-16 to the cytoplasm....
Article
Full-text available
Stem cell-derived sheet engineering has been developed as the next-generation treatment for myocardial infarction (MI) and offers attractive advantages in comparison with direct stem cell transplantation and scaffold tissue engineering. Furthermore, induced pluripotent stem cell-derived cell sheets have been indicated to possess higher potential fo...
Article
Full-text available
The application of mechanical stimuli to cells often induce increases in intracellular calcium, affecting the regulation of a variety of cell functions. Although the mechanism of mechanotransduction-induced calcium increases has not been fully resolved, the involvement of mechanosensitive ion channels in the plasma membrane and the endoplasmic reti...
Article
Transcriptional regulation is crucial for neuronal activity-dependent processes that govern neuronal circuit formation and synaptic plasticity. An intriguing question is how neuronal activity influences the spatiotemporal interactions between transcription factors and their target sites. Here, using a single-molecule imaging technique, we investiga...
Article
Significance statement: The transcription factor, CREB (cAMP response element-binding protein) is known to regulate gene expression in neuronal activity-dependent processes. However, its spatiotemporal interactions with the genome remain unknown. Single-molecule imaging in cortical neurons revealed that fluorescent-tagged CREB spots frequently res...
Article
Integrins mediate cell adhesion to the extracellular matrix and enable the construction of complex, multicellular organisms, yet fundamental aspects of integrin-based adhesion remain poorly understood. Notably, the magnitude of the mechanical load experienced by individual integrins within living cells is unclear, due principally to limitations inh...
Article
Integrins are heterodimeric transmembrane proteins that mediate cell attachment to the extracellular matrix (ECM). Mammalian cells express numerous α/β-integrin heterodimers, each with a unique combination of affinities for individual ECM components and distinct roles in intracellular signal transduction. Notably, αvβ3, αvβ5, and α5β1 integrins rec...
Article
Full-text available
Proper spatiotemporal gene expression is achieved by selective DNA binding of transcription factors in the genome. The most intriguing question is how dynamic interactions between transcription factors and their target sites contribute to gene regulation by recruiting the basal transcriptional machinery. Here we demonstrate individual binding and d...
Article
Full-text available
The structural dynamics of actin, including the tilting motion between the small and large domains, are essential for proper interactions with actin-binding proteins. Gly146 is situated at the hinge between the two domains, and we previously showed that a G146V mutation leads to severe motility defects in skeletal myosin but has no effect on motili...
Article
Focal adhesions (FAs) are micron-sized protein assemblies that coordinate cell adhesion, migration, and mechanotransduction. How the many proteins within FAs are organized into force sensing and transmitting structures is poorly understood. We combined fluorescent molecular tension sensors with super-resolution light microscopy to visualize tractio...
Article
Near-infrared (NIR) fluorescence imaging in wavelengths from 1000 to 1500 nm (2nd-NIR window) is a promising modality for in vivo fluorescence imaging because of the deeper tissue penetration with lower tissue scattering of the 2nd-NIR light. For such in vivo fluorescence imaging, highly fluorescent probes in the 2nd-NIR wavelength region are neede...
Article
Mechanical interactions between cells and the extracellular matrix (ECM) exert a profound influence on cell migration, proliferation, and stem-cell differentiation. However, how cells generate and detect mechanical force remains poorly understood, in part due to a lack of methods that visualize molecular-scale forces in living cells. Here we descri...
Article
Living cells are exquisitely responsive to mechanical cues, yet how cells produce and detect mechanical force remains poorly understood due to a lack of methods that visualize cell-generated forces at the molecular scale. Here we describe Förster resonance energy transfer (FRET)-based molecular tension sensors that allow us to directly visualize ce...
Article
Mechanical interactions between cells and the extracellular matrix (ECM) exert a profound influence on cell migration, proliferation, and stem cell differentiation. However, fundamental aspects of how cells detect and generate mechanical forces at the cell-ECM interface remain poorly understood. Here we describe a new technique, termed Molecular Fo...
Article
Epithelial cells transmit mechanical forces through E-cadherin-mediated intercellular contacts, and these dynamic complexes undergo force-dependent remodeling. At present, however, fundamental aspects of how cells detect and generate mechanical forces at intercellular junctions remain poorly understood. Here, we describe a FRET-based molecular forc...
Article
Full-text available
Transformations between G- (monomeric) and F-actin (polymeric) are important in cellular behaviors such as migration, cytokinesis, and morphing. In order to understand these transitions, we combined single-molecule Förster resonance energy transfer with total internal reflection fluorescence microscopy to examine conformational changes of individua...
Data
The effects of phalloidin on F-actin. Distribution of the FRET efficiencies in F-actin without phalloidin is shown (cf. Fig. 2C). High ionic strength increased the fg state of F-actin. In the absence of phalloidin, G-actin depolymerized from the filament and adhered to the glass surface non-specifically, affecting the distribution of its states. (T...
Data
Details of statistical analysis of the Förster resonance energy transfer (FRET) states and molecular dynamics simulations. (DOCX)
Data
Schematic diagram of the experimental setup. (TIF)
Data
Comparisons of non-specific adhesion of G-actin to the glass surface via an anti-myc-antibody in assay buffer with 150 mM KCl. Distribution of the FRET efficiencies in non-specifically adhered G-actin is shown. (cf. Fig. 2B). Non-specific adhesion increased the probability of the g state, as compared to the specifically binding case using antibody....
Data
Time series of the corresponding distance observed in molecular dynamics simulations. The distance between the α-carbon atoms in residues 41 and 374 is shown. (G) an isolated G-actin monomer and (F) an F-actin model (pentamer). (TIF)
Data
Distribution of G and F-actin Förster resonance energy transfer (FRET) efficiencies. Additional data (not shown in Fig. 2) is shown. Color codes correspond to the classification in the statistical analysis shown in Text S1 and Table S1. (TIF)
Data
Distribution of Förster resonance energy transfer (FRET) states in each condition. (DOC)
Article
In the lever arm model for actomyosin motility, the only roles of actin filaments are to stimulate Pi release from myosin and to provide foothold for tension generation. However, actin filaments undergo myosin-dependent cooperative conformational changes. To understand the possible functions of those changes in actomyosin motility, we hoped to obta...
Article
One of the most abundant proteins in eukaryotes is actin, a ubiquitous protein that plays a role in cell dynamics like cell migration. The dynamics of actin filament treadmilling is regulated by two actin structural states: globular actin (G-actin) and filamentous actin (F-actin). While G-actin's crystal structure has been solved by several groups,...
Article
Until recently, actin was thought to act merely as a passive track for its motility partner, myosin, during actomyosin interactions. Yet a recent report having observed dynamical conformational changes in labeled skeletal muscle alpha-actin suggests that actin has a more active role. Because the labeling technique was still immature, however, concl...
Article
A novel pattern transfer technique has been demonstrated by simple procedure based on a nanoimprint lithography. A polymer is coated on a mold by a spin coater and cast into fine grooves on the mold. After evaporation of a solvent, an adhesive resin is coated on the polymer and put a substrate plate. After releasing the mold, fine pattern is succes...
Article
The fabrication of a three-dimensional multilayered microstructure is demonstrated by the imprint and reversal imprint technique. We have studied polymer properties and pressing conditions as important parameters for selecting the reversal imprint modes. We investigated the conditions that are required to form the fluid channels and the through hol...
Article
A simple microfabrication method for a controlled-release drug-delivery system has been designed using biodegradable polymeric microchips. Microholes were made in a poly(L-lactic acid) plate and dyes were cast in each well. After drying, the wells were sealed with polymers having different biodegradation rates using a mold that had hollows correspo...
Article
Three dimensional micro-nano structures have been achieved to fabricate by nano imprint lithography and advanced reversal imprint technology using thermal polymer. Fine grating structures is fabricated by conventional imprint lithography on a polymer surface. A spin coated thin polymer film on mold plate is pressed onto the grating structure and th...

Network

Cited By

Projects

Project (1)