Masako Fujioka-Kobayashi

Shimane University · Department of Oral and Maxillofacial Surgery

BACKGROUND Injectable platelet-rich fibrin (iPRF), a liquid form of PRF that is prepared from peripheral blood without anticoagulants, promotes tissue wound healing and regeneration. The present study focused on iPRF-like bone marrow aspirate concentrate (iBMAC) prepared without anticoagulant, and the regenerative potential of iPRF and iBMAC was co...

Background Surgical guides manufactured with computer‐aided design/computer‐aided manufacturing (CAD/CAM) technology are effective tools for oral surgeries. Case presentation A new type of surgical guide with mucoperiosteal flap elevation function was custom designed, printed, and applied for the safe and accurate removal of six impacted supernume...

Objectives Collagen membranes are extensively used for guided bone regeneration procedures, primarily for horizontal bone augmentation. More recently, it has been demonstrated that collagen membranes promote bone regeneration. Present study aimed at assessing if structural modifications of collagen membranes may enhance their osteoconductive capaci...

Gradual elevation of the periosteum from the original bone surface, based on the principle of distraction osteogenesis, induces endogenous hard and soft tissue formation. This study aimed to assess the impact of alternating protocols of activation with relaxation (periosteal pumping) on bone modeling and remodeling. One hundred and sixty-two adult...

Bone grafts are typically categorized into four categories: autografts, allografts, xenografts, and synthetic alloplasts. While it was originally thought that all bone grafts should be slowly resorbed and replaced with native bone over time, accumulating evidence has in fact suggested that the use of nonresorbable xenografts is favored for certain...

The goals of the study were to investigate the effects on bone bioactivity of a titanium dioxide layer formed by hydrothermal oxidation of a titanium surface with hydrogen peroxide（H2O2） and loading with fibroblast growth factor-2（FGF-2） in vitro and in vivo. Ti-6Al-4V discs were hydrothermally oxidized with H2O2 and then loaded with FGF-2. After c...

The use of platelet‐rich fibrin (PRF) has gained tremendous popularity in recent years owing to its ability to speed wound healing postsurgery. However, to date, many clinicians are unaware of methods designed to optimize the technology. This overview article will discuss the advancements and improvements made over the years aimed at maximizing cel...

Nasopalatine cysts are common nonodontogenic cysts that occur in the maxilla. During the nucleation of large cysts extending to the floor of the nasal cavity, care must be taken to avoid damage to the nasal mucosa. In the present report, an innovative custom-made surgical guide made by a Three-dimensional printer is introduced for accurate enucleat...

Solid bone marrow aspirate concentrate (sBMAC) is harvested from bone marrow aspirate without anticoagulants by a centrifugation protocol similar to that for platelet-rich fibrin (PRF) prepared from peripheral blood. It was hypothesized that sBMAC could accelerate not only wound healing but also bone regeneration because of the abundant growth fact...

The goals of the study were to investigate the effects on bone bioactivity of a titanium dioxide layer formed by hydrothermal oxidation of a titanium surface with hydrogen peroxide (H2O2) and loading with fibroblast growth factor‐2 (FGF‐2) in vitro and in vivo. Ti‐6Al‐4V discs were hydrothermally oxidized with H2O2 and then loaded with FGF‐2. After...

(1) Aim: To investigate the effect of synthetic bone substitutes, α-tricalcium phosphate (α-TCP) or bi-layered biphasic calcium-phosphate (BBCP) combined with deproteinized bovine bone mineral (DBBM), on bone formation. (2) Methods: Thirty critical size defects were randomly treated with the following five different treatment modalities: (1) negati...

The present study investigated the osteoclast differentiation potential and paracrine effects of osteoclasts on osteoblast differentiation when the cells were cultured directly on two bone substitutes (BSs): deproteinized bovine bone mineral (DBBM) and carbonate apatite (CO3Ap). Human primary osteoclasts cultured on the BSs were assessed by tartrat...

Platelet-rich fibrin (PRF) prepared from venous blood is used in the clinic to improve soft tissue wound healing. Nevertheless, arterial blood or bone marrow aspirate might also be a candidate for the source of PRF-like concentrates. The purpose of the present study was to investigate blood/bone marrow aspirate concentrates obtained from arterial b...

Objectives We previously showed that accelerated degradation of collagen membranes (CMs) in diabetic rats is associated with increased infiltration of macrophages and blood vessels. Since pre-implantation immersion of CMs in cross-linked high molecular weight hyaluronic acid (CLHA) delays membrane degradation, we evaluated here its effect on the nu...

Brain abscesses are mainly caused by pyogenic bacteria such as staphylococci and streptococci. The oral bacteria Streptococcus intermedius, which has attracted attention in recent years because of its pathogenicity and tendency to cause infections in the central nervous system, is remotely transferred. We report a case of a brain abscess caused by...

The aim of the present study was to investigate the effects of recombinant human (rh)BMP-9 on bone regenerative potential in a mouse model of antibody-mediated antiresorptive therapy (AMART). A monoclonal anti-murine receptor activator of nuclear factor-kappa B ligand (RANKL) antibody (mAb) was used to create an AMART model in mice. rhBMP-9 combine...

Purpose: To investigate the effect of platelet-rich fibrin on bone formation by investigating its use in guided bone regeneration, sinus elevation and implant therapy. Materials and methods: This systematic review and meta-analysis were conducted and reported in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analys...

Objective Bone substitute (BS) size might influence the clinical outcomes of guided bone regeneration (GBR) procedures. The aim of the present study was to investigate the influence of BS size on macrophage (Mφ) and osteoblast behaviors in vitro. Materials and methods Two different granule sizes (S and M/L) were assessed for four different commerc...

Alveolar bone defects caused by tooth loss often lead to challenges in implant dentistry, with a need for development of optimal bone biomaterials to predictably rebuild these tissues. To address this problem, we fabricated a novel bone block using platelet-rich fibrin (PRF) and Deproteinized Bovine Bone Mineral (DBBM), and characterized their mech...

The cross-linking of collagen matrices (Cl_CM) may provide volume-stable enhanced defect regeneration when compared to non-cross-linked matrices (Ncl_CM). The aim of the present study was to investigate the bone forming potential of collagen matrices (CMs) and the effects of cross-linking CMs in a rabbit calvaria defect model. (1) Empty controls (n...

Purpose: To investigate the use of platelet-rich fibrin for alveolar ridge preservation compared to natural healing, bone graft material and platelet-rich fibrin in combination with bone graft material. Materials and methods: The present systematic review was conducted and reported according to the Preferred Reporting Items for Systematic Review...

Objectives This study aims to compare the treatment outcomes of periodontal intrabony defects by using platelet-rich fibrin (PRF) with other commonly utilized modalities. Materials and methods The eligibility criteria comprised randomized controlled trials (RCTs) comparing the clinical outcomes of PRF with that of other modalities. Studies were cl...

Accelerated degradation of collagen membranes (CMs) in diabetic rats is associated with increased infiltration of macrophages and blood vessels. Since pre-implantation immersion of CMs in cross-linked high molecular weight hyaluronic acid (CLHA) delays membrane degradation, we evaluated its effect on the number of macrophages and endothelial cells...

Objective To investigate the efficacy of platelet-rich fibrin (PRF) for the socket healing after removal of mandibular third molars when compared to natural wound healing. Methods This systematic review and meta-analysis were conducted according to PRISMA guidelines. The eligibility criteria comprised randomized controlled trials (RCTs) comparing...

Background Platelet-rich fibrin (PRF) has been widely utilized in modern medicine and dentistry owing to its ability to rapidly stimulate neoangiogenesis, leading to faster tissue regeneration. While improvements over traditional platelet rich plasma therapies (which use chemical additives such as bovine thrombin and calcium chloride) have been obs...

The aim of this study was to evaluate the influence of additional hydroxyapatite (HA) in collagen-based matrices (CM) and membrane placement on bone formation in calvarial defects. Critical size defects in the calvaria of 16 New Zealand White Rabbits were randomly treated with CM or mineralized collagen-based matrices (mCM). Half of the sites were...

The aim of this study was to evaluate the influence of the intensity of the biomimetic hydroxyapatite (HA) coating of α-tricalcium phosphate (α-TCP) on biomaterial degradation and bone formation. Twenty-four female NZW rabbits of approximately 12 weeks of age were used. Critical size defects were randomly treated with 3%:97% HA:α-TCP (BBCP1), 12%:8...

Much can be discussed with respect to the biology of PRF and its ability to impact tissue regeneration. During the natural wound healing process, vascularization of tissues plays a pivotal role, facilitating the invasion of incoming cells, growth factors (GFs), cytokines, and other regenerative factors. The main aim of platelet concentrates discove...

The first two chapters of this textbook discussed the history of platelet concentrates and their commercial and biologic evolution. The aim of this chapter is to discuss centrifugation parameters that may best optimize the production of PRF. A great deal of work has been conducted at both the preclinical and clinical level to maxi-mize the regenera...

This chapter is a relatively short one designed to simplify proto-cols for the production of PRF. In general, only three protocols are necessary for PRF therapy. The first is the standard PRF membrane in which ideally a higher platelet and leukocyte yield is harvested with an even distribution of cells within the upper 4- to 5-mL PRF layers. This i...

It is hard to imagine that the effect of centrifugation tubes may in fact be more relevant for the production of PRF than the actual centrifugation device. Tubes, which are generally purchased and range in price from $0.85 to $1.55 each, have a massive implication in the final outcomes of both solid-PRF and liquid-PRF. Simply, more hydrophilic tube...

One of the main reported drawbacks of PRF use is its rather fast resorption properties characterized within a 2-to 3-week time frame. However, recent studies have demonstrated that by heating a liquid platelet-poor plasma (PPP) layer, the resorption properties of heated albumin (albumin gel) can be significantly extended from 2 weeks to more than 4...

Today, the use of PRF has been growing in the field of oral and maxillofacial surgery. Many studies have now demonstrated the effectiveness of local application of PRF as an adjunctive therapy to surgical protocols for the management of various common defect types, such as osteonecrosis of the jaw (ONJ). The first aim of this chapter is therefore t...

Various bone grafting materials are used for bone augmentation procedures in the field of oral-maxillofacial surgery. Knowledge of biomaterial characteristics is crucial in order to choose the appropriate materials for each clinical case. The purpose of the present study was to investigate not only the conventional method of comparison of osteogeni...

Objectives Several studies have recently demonstrated that only marginal improvements in platelet and leukocyte concentrations are achieved following standard injectable platelet-rich fibrin (i-PRF) protocols. Due to these previous findings, a novel harvesting technique was recently developed to collect higher concentrations of platelets/leukocytes...

Objective This study correlates the mechanical and biological response of commercially available resin-based composites (RBCs) to clinically relevant light-curing conditions. Methods Two RBCs (Venus and Venus Pearl; Kulzer) that use different monomer and photo-initiator systems, but have a similar filler volume and shade, were exposed to either ju...

Background The aim of this study was to evaluate 24 protocols for the production of platelet rich fibrin (PRF) produced via horizontal centrifugation to better understand cell separation following protocols at various times and speeds. Methods All protocols were compared utilizing a recent method to quantify cells in PRF in 1 mL sequential layers...

Objectives Biphasic calcium phosphates (BCP) are synthetic biomaterials developed as an alternative to the autogenous bone grafts and xenografts. The aim of the present study was to assess the influence of the addition of collagen onto the BCP resorption rate and bone formation. Material and methods Eighteen male NWZ rabbits approximately 12 weeks...

Background and objectives Liquid platelet rich fibrin (PRF; often referred to as injectable PRF) has been utilized as an injectable formulation of PRF that is capable of stimulating tissue regeneration. Our research group recently found that following standard L-PRF protocols (2700 RPM for 12 min), a massive increase in platelets and leukocytes was...

Objectives The aim of this systematic review and meta-analysis was to compare the use of platelet-rich fibrin (PRF) with other commonly utilized treatment modalities for root coverage procedures.Materials and methodsThe eligibility criteria comprised randomized controlled trials (RCTs) comparing the performance of PRF with that of other modalities...

Most available antiseptic solutions available today have strong antibacterial effects, however most also possess major cytotoxic effects on human gingival tissues. The VEGA Oral Care Recovery Kit (StellaLife), previously evaluated in clinical studies, consists of 16 active ingredients that are monographed in the Homeopathic Pharmacopeia of United S...

Deproteinized bovine bone mineral (DBBM) bone grafts are commonly utilized for guided bone regeneration (GBR) techniques in regenerative dentistry. It has been hypothesized that different forms (blocks versus particulates) might demonstrate the varying properties of cell behavior during the regenerative process. Therefore, the aim of the present st...

Platelet-rich fibrin (PRF) is prepared from whole blood without any exogenous coagulation factors. Several preparation methods have now been introduced, particularly with differences in centrifugation parameters including g-force and time to improve their regenerative potential. Nevertheless, the centrifugation systems have not yet been clearly inv...

Background The aim of this study was to evaluate 24 protocols for the production of platelet rich fibrin (PRF) produced via horizontal centrifugation to better understand cell separation following protocols at various times and speeds. Methods All protocols were compared utilizing a recent method to quantify cells in PRF in 1 mL sequential layers p...

Collagen biomaterials are widely used for soft tissue augmentation. Cross-linking techniques for collagen matrix (CM) achieve mechanical and volumetric stability; nevertheless, cross-linking may compromise biocompatibility. The aim of the present study was to investigate two different three-dimensional (3D) porcine-derived CMs, non-crosslinked (ncl...

Platelet-rich fibrin (PRF) has been proposed as an autologous membrane with the advantages of host accumulation of platelets and leukocytes with entrapment of growth factors. However, limitations include its faster resorption properties (~2 weeks). Interestingly, recent studies have demonstrated that by heating a liquid platelet-poor plasma (PPP) l...

Background The aim of this study was to evaluate 24 protocols for the production of platelet rich fibrin (PRF) produced via horizontal centrifugation to better understand cell separation following protocols at various times and speeds. Methods All protocols were compared utilizing a recent method to quantify cells in PRF in 1 mL sequential layers p...

Background The aim of this study was to evaluate 24 protocols for the production of platelet rich fibrin (PRF) produced via horizontal centrifugation to better understand cell separation following protocols at various times and speeds. Methods All protocols were compared utilizing a recent method to quantify cells in PRF in 1 mL sequential layers p...

Background: The aim of this study was to evaluate 24 protocols for the production of platelet rich fibrin (PRF) produced via horizontal centrifugation to better understand cell separation following protocols at various times and speeds. Methods: All protocols were compared utilizing a recent method to quantify cells in PRF in 1 mL sequential layers...

Platelet concentrates have seen a steady rise in use in various fields of medicine as a natural autogenous source of growth factors derived from human peripheral blood. While platelet-rich plasma (PRP) was proposed as a first-generation platelet concentrate over three decades ago, over the past 10 years platelet-rich fibrin (PRF) has been introduce...

Calvarial bone surgery on rabbits is frequently performed. This report aims to document a simple and practical anaesthetic and perioperative management for this procedure. Fourteen male New Zealand white rabbits were included in the study. Subcutaneous (SC) dexmedetomidine, ketamine and buprenorphine ± isoflurane vaporized in oxygen administered th...

Abstract Recombinant human bone morphogenic protein (rhBMP) 9 has recently been reported to have more osteopromotive potential in vitro when compared to rhBMP2. The aim of the present study was to investigate the bone‐inducing potential of rhBMP2 and rhBMP9. We compared rhBMP2, rhBMP7, and rhBMP9 at five different concentrations and showed convinci...

Objective: This study investigated the influence of the degree of conversion (DC), resin-based composites (RBC) composition, and the effect of additional violet light from one light curing unit (LCU) on cell attachment/growth, eluate cytotoxicity, and gene expression. Methods: The effect of different DC of RBCs on human gingival fibroblasts (HGF...

Aim To examine the in vitro biokinetics of hyaluronic acid (HA) from a collagen membrane (CM) and to evaluate the in vivo effect of immersion of the CM in HA solution on its degradation in streptozotocin (STZ)‐induced diabetes conditions in a rat calvaria subcutaneous model. Background CM degradation is accelerated in uncontrolled diabetic rats. I...

Objectives Recently, a new liquid carrier system for enamel matrix derivate (EMD‐liquid) was developed with better physico‐chemical properties for improved adsorption of EMD to biomaterial surfaces. The aim of the present study was to investigate the bone regenerative potential of EMD‐liquid in combination with a natural bone mineral (NBM) in vivo....

Objectives: Guided bone / tissue regeneration (GBR/GTR) procedures are necessary to improve conditions for implant placement. These techniques in turn can be enhanced by using growth factors (GFs) such as bone morphogenetic protein (BMP-2) and platelet-derived growth factor (PDGF) to accelerate regeneration. The aim of the present systematic revie...

Platelet concentrates have had a long history of use in various fields of dentistry and medicine as an autogenous source of growth factors derived from human peripheral blood. While platelet-rich plasma (PRP) was proposed as a first-generation platelet concentrate over three decades ago, over the past 10 years, platelet-rich fibrin (PRF) has seen a...

Hyaluronic acid (HA) has been utilized for a variety of regenerative medical procedures due to its widespread presence in connective tissue and perceived biocompatibility. While its use in various fields of medicine has provided encouraging data supporting its clinical use, the dental field has only recently utilized HA as a regenerative modality....

Since recombinant human bone morphogenetic protein 2 (rhBMP-2) was first introduced to the market as a recombinant human growth factor with US Food and Drug Administration (FDA) approval for bone regeneration, it has blossomed into an over $750 million per year industry. Its main advantage is its ability to rapidly stimulate new bone formation , an...

Objectives: The purpose of this study was to compare the effects of rhBMP2 with rhBMP9 on ridge augmentation following healing of extraction sockets in dogs. Material and methods: Five male Beagle dogs, approximately 12 months of age, were used. The mesial roots of the four maxillary premolars were endodontically treated. The distal roots were e...

The cover image is based on the Original Research Recombinant human BMP9 (RhBMP9) in comparison with rhBMP2 for ridge augmentation following tooth extraction: An experimental study in the Beagle dog by Nikola Saulacic et al., DOI: 10.1111/clr.13371.

Carbonate apatite‐coated calcium carbonate (CO3Ap/CaCO3) was fabricated through a dissolution–precipitation reaction using CaCO3 granules as a precursor to accelerate bone replacement based on superior osteoconductivity of the CO3Ap shell, along with Ca²⁺ release from the CaCO3 core and quicker resorption of the CaCO3 core. In the present study, Ca...

This study aimed to evaluate the impact of three types of block bone substitute material on bone formation and graft resorption in vivo. Standardized bone defects (n = 4 defects/animal) were created in the calvaria of nine dogs. Block bone substitutes made of deproteinized bovine bone mineral (DBBM), beta-tricalcium phosphate (β-TCP) and a mixture...

Objectives: Among bone morphogenetic protein (BMP) fam- ily members, BMP-2 and BMP-9 have demonstrated potent osteoinductive potential. However, in vivo differences in their potential for bone regeneration remain unclear. The present study aimed to compare the effects of recombinant human (rh) BMP-2 and rhBMP-9 on bone formation in rat calvarial cr...

Objectives: Platelet rich plasma (PRP) has been utilized in regenerative dentistry as a supra-physiological concentrate of autologous growth factors capable of stimulating tissue regeneration. Despite this, concerns have been expressed regarding the use of anti-coagulants, agents known to inhibit wound healing. In this study, a liquid formulation...

Antiseptic solutions are commonly utilized to treat local infection in the oral and maxillofacial region. However, surrounding vital bone is also exposed to antiseptic agents during irrigation and may have a potential negative impact on bone survival. The aim of the present study was therefore to investigate the effect of rinsing time with various...