Maryline Vian

University of Avignon | UAPV · Green

The extraction of oil from oilseeds is conventionnaly performed using n-hexane. However, due to its toxicity and drawbacks, there is a growing need to explore greener solvents. In this study, 2-methyloxolane (2-MeOx) was selected to evaluate its potential as a replacement for n-hexane in extracting lipophilic compounds from Pistacia lentiscus seeds...

Microalgae have been widely recognized as a promising feedstock for sustainable biofuels production to tackle global warming and pollution issues related to fossil fuels uses. This study identified and analyzed indigenous microalgae strains for biodiesel production, specifically Chlorella vulgaris and Coelastrella thermophila var. globulina , from...

Pistacia lentiscus L. fruits are an excellent source of phenolic compounds endowed with antioxidant activities. Ethanol-water mixtures are in focus for plant extracts preparation due to their acceptability for human consumption. In this study, the effect of ethanol concentration in ethanol/water (EtOH/H 2 O) extraction solvent on the solubility of...

The vegetable oil world market is constantly growing to meet both the food needs of a growing population and the increasing demand for biofuels, the latter due to rising petroleum prices. The recovery of vegetable oils from oilseeds, commonly called “crushing,” is usually a four step process that successively includes: (i) pre-treatment of the seed...

Hexane is a solvent used extensively in the food industry for the extraction of various products such as vegetable oils, fats, flavours, fragrances, colour additives or other bioactive ingredients. As it is classified as a “processing aid”, it does not have to be declared on the label under current legislation. Therefore, although traces of hexane...

Hexane is a solvent used extensively in the food industry for the extraction of various products such as vegetable oils, fats, flavours, fragrances, colour additives or other bioactive ingredients. As it is classified as a "processing aid", it does not have to be declared on the label under current legislation. Therefore, although traces of hexane...

The black soldier fly larvae (BSFL) is a sustainable ingredient for feed applications, biofuels, composite materials and other biobased products. Processing BSFL to obtain lipid and protein fractions with enhanced functional properties as a suitable replacement for conventional feed ingredients has gained considerable momentum. In this regard, a no...

Analytical chemistry relies massively on solvents, which are generally petroleum-based and have hazardous effects. The ideal alternative solvents suitable for analysis should have high selectivity, solvency, low toxicity, and low environmental impacts, be easily biodegradable and recyclable, and be obtained from renewable resources at a reasonable...

Edible oils are currently largely obtained by solvent extraction using hexane. Despite the fact that this petroleum solvent is known to be neurotoxic, hexane extraction remains the accepted standard process for seed oil extraction and particularly, but not exclusively, for soybean oil extraction. This study evaluates an alternative replacement of h...

The orange tree (Citrus sinensis) is a species of fruit shrubs of the Rutaceae family. Essential oils from citrus peels are widely used in various agro-food and pharmaceutical preparations and hence they must be recovered using green extraction methods. In this study, we compare the efficiency of five extraction techniques (conventional hydro-disti...

Depletion of fossil resources, global warming, and increasing world’s population represents major Damocles' sword for humanity to ensure its future against famine, climate change and the end of the petroleum era. Solutions will come from production of plant-based chemicals which is not new, nor a historical artefact. On earth, 99% of the biomass al...

In this 21st century, much interest has been focused on the applications of ultrasound in food processing and technology. Applications of ultrasound generally involve processes that can enhance rates, improve quality and/or safety, reduce processing time, and play a key role in implementing sustainable “green” technology. Ultrasound is applied in o...

The emergence of black soldier fly larvae (BSFL) as one among the vital tools for generating circular economy has enabled its use in several applications such as biorefining, valorization of waste management, treatment of industrial by-products, and bioconversion of agro-industrial residues. The ability of BSFL to assimilate chemical constituents o...

This review presents a complete picture of current knowledge on 2-methyloxolane (2-MeOx), a bio-based solvent for the extraction of natural products and food ingredients. It provides the necessary background about the properties of 2-MeOx, not only its solvent power and extraction efficiency, but its detailed toxicological profile and environmental...

Each year, the food supply chain produces more than 1.3 billion tons of food and agricultural waste, which poses serious environmental problems. The loss of the massive quantity of secondary and primary metabolites retrievable from this resource is a significant concern. What if there is a global solution that caters to the numerous problems arisin...

Ultrasound-assisted extraction is widely recognized as an eco-friendly technique due to low solvent consumption and time extraction as well as enhanced extraction efficiency with respect to conventional methods. Nevertheless, it would be convenient to avoid the usually used organic solvents to reduce the environment pollution. In this regard, Deep...

Analytical techniques are employed every day in both, industry and academia. The concept of green analytical chemistry involves making analytical chemistry safer for operators, more sustainable for the environment and more economical. Improvements in the availability of renewable feedstocks, miniaturization, automated technology, and chemical recyc...

This review presents innovative extraction techniques and their role in promoting sustainable ingredients for the food, cosmetic and pharmaceutical industries. These techniques (such as microwave, ultrasound, pulse electric field, instant controlled pressure drop, sub- and super-critical fluid processing, extrusion, mechanochemistry, high pressure,...

The potential of 2-methyloxolane (2-MeO) as an alternative solvent to extract cactus seed oil was compared, in qualitative and quantitative terms, with that of n-hexane, a solvent commonly used for the extraction of edible or cosmetic oils. With 2-MeO, the oil yield was higher (9.55 ± 0.12 g/100 g) than the oil extracted with n-hexane (8.86 ± 0.25...

In recent years, almost all extraction processes in the perfume, cosmetic, pharmaceutical, food ingredients, nutraceuticals, biofuel and fine chemical industries rely massively on solvents, the majority of which have petroleum origins. The intricate processing steps involved in the industrial extraction cycle makes it increasingly difficult to pred...

Scrutiny of alternative solvents for the extraction of lipid constituents from black soldier fly (BSF) was the main theme of this research. The present investigation compared a wide array of solvents for the extraction of desired components theoretically using tools like Hansen solubility parameters (HSP), conductor-like screening model for real so...

Biorefinery is a concept inspired from traditional oil refinery for petrol production, which enables to generate numerous bioproducts without any wastes from a unique source. This idea fits in with the actual context of sustainable development, where every part of the biomass treated is valorized through different unit operations in an economic and...

This study assesses, qualitatively and quantitatively, the performance of ten bio-based solvents to replace hexane for the extraction of oil from Pistacia lentiscus edible fruits. Solvent screening was achieved through theoretical approach using the COnductor-like Screening MOdel for Realistic Solvation (COSMO-RS) predictions and an experimental pr...

Green extraction of natural products is based on design of extraction processes which will reduce or eliminate energy consumption and petroleum solvents, while ensuring a safe extract and quality. It is a concept to meet the challenges of the 21st century protecting both our environment and consumers, and in the meantime, enhance competition of aca...

This study aimed to evaluate the extraction efficiency of six alternative solvents compared to hexane, conventionally used, for extraction of aromas from herbs and spices. It has been performed through theoretical approach using conductor-like screening model for real solvents (COSMO-RS) as predictive method and experimental approach based on gas c...

Essential oils (EOs) are widely used in the perfume, cosmetic, pharmaceutical, agricultural, and food industries. These have long been recognized since antiquity to possess biological activities, including antioxidant, antibacterial, antifungal, antiviral, antimycosic, antitoxigenic, antiparasitic, and insecticidal properties. In recent years, a la...

Bio-refining of bilberry pomace using innovative technologies such as microwave hydro diffusion and gravity extraction (MHG) and ultrasound assisted extraction (UAE) with different concentrations of ethanol/water as the solvent was established. Bead milling was also utilized in this sequential extraction scheme to remove the lipophilic fraction. So...

Bligh and Dyer (B & D) or Folch procedures for the extraction and separation of lipids from microorganisms and biological tissues using chloroform/methanol/water have been used tens of thousands of times and are “gold standards” for the analysis of extracted lipids. Based on the Conductor-like Screening MOdel for realistic Solvatation (COSMO-RS), w...

An analytical procedure comprises two steps: sample preparation mostly extraction (Soxhlet, Dean-Stark, Likens-Nickerson, Blye&Dyer, Folch, leaching, maceration, percolation) and analysis (spectrophotometry, chromatography, spectrometry). Whereas chemical analysis is finished after few minutes, extraction takes at least several hours and require la...

The ideal solvent for extraction is no solvent. This chapter presents innovative techniques convenient to perform extraction very efficiently in the absence of any organic solvents, so-called dry media conditions. The advantages of using solvent-free conditions reach from faster extraction with a different selectivity to more economical conditions...

Manothermonication is a recognized and efficient method used for sterilization in food preservation. The synergistic effect of sonication combined with pressure and temperature allows enhancing the cavitation activity. Never employed for extraction, this study is about the transposition of this process as a tool of extraction. In this study, Rhodos...

Oleaginous microbial sample is added to a mixture of methanol, chloroform and water in a three step extraction. This principle is well known as Folch or Bligh and Dyer (REF) procedure for the extraction and separation of lipid-rich tissues. Despite their high efficiency for extraction of lipids from microbial tissue, solvents involved in those proc...

This article deals with the extraction of neutral lipids and antioxidants from enriched Spirulina platensis microalgae using supercritical carbon dioxide (CO2), and more particularly with the influence of experimental conditions on extraction yields and kinetics at laboratory and pilot scales. Preliminary studies were carried out at laboratory scal...

Lipid-based oleaginous microorganisms are potential candidates and resources for the sustainable production of biofuels. They can accumulate 20% to 70% of their dry cell mass as lipids [1][2] converting carbon sources contained in various substrates. Currently, technique for extraction of lipids from microorganisms use petroleum solvents, such as h...

In the present work, two different ways of lipids extraction from Yarrowia lipolytica yeast were investigated in order to maximize the extraction yield. Firstly, various modern techniques of extraction including ultrasound, microwave, and bead milling were tested to intensify the efficiency of lipid recovery. Secondly, several pretreatments such as...

Lipid-based oleaginous microorganisms are potential candidates and resources for the sustainable production of biofuels. This study was designed to evaluate the performance of several alternative bio-based solvents for extracting lipids from yeasts. We used experimental design and simulation with Hansen solubility simulations and the conductor-like...

Ultrasound-assisted extraction of rapeseed oil was investigated and compared with conventional extraction for energy efficiency, throughput time, extraction yield, cleanness, processing cost and product quality. A multivariate study enabled us to define optimal parameters (7.7 W/cm2 for ultrasonic power intensity, 40 °C for processing temperature,...

In the past few years, much interest has focused on the applications of ultrasound in food processing and technology. Many reports suggest effective benefits from combining ultrasound with classical techniques. Applications of ultrasound generally involve processes that can enhance rates, improve quality and/or safety, and reduce processing time. U...

Background: Phycocyanin (PC) is a major light harvesting pigment of cyanobacteria. It has gained importance in many biotechnological applications in food sciences, therapy, cosmetics and pharmacological processes thanks to its blue color and to its properties. Arthrospira platensis, also called blue algae, and commonly known as spirulina, is very i...

Rapeseed oil is at the third place of the most consumed vegetable oils worldwide after palm and soya and is at the first place in Europe and France. The implementation of rapeseed oil process includes several steps such as preparation of seeds, mechanical extraction by pressing and solvent extraction of the press cake. This solvent extraction step...

Oleaginous microorganisms such as yeast, bacteria, and microalgae, are increasingly studied as an alternative source of lipids owning high potential due to their productivity and rapid growth. Some species of oleaginous microorganisms are able to accumulate more than 20 % of their dry cell mass in the form of lipids [1]. In a particular and optimiz...

Mass production of microalgae worldwide, and even more so in developing countries, is strongly contingent upon the availability of economical and efficient photobioreactors (PBRs) that are amenable for use in resource-limited environments. Such options are limited. This work assesses the effects of temperature, CO2 enrichment, and mixing by air + C...

A valuable reference presenting many processes that facilitate lipid extraction from micro-organisms. Amongst the techniques included are Folch, Bligh and Dyer methods, and the Soxhlet technique as well as intensified green processes (ultrasound, microwave, supercritical fluid extraction, agro-solvent, accelerated solvent extraction, enzyme-assiste...

The use of innovative extraction techniques such as ultrasound, microwave, instant controlled pressure drop, supercritical fluid, pulsed electric fields, mechanical pressing, agro-solvent and enzyme assisted extraction (Table 4.1) allows total recovery of lipids from microorganisms and also reduced extraction time, energy consumed, and less solvent...

This chapter reviews the development of extraction and analytical techniques for lipids from microorganisms. The commonly used extraction techniques of total lipid such as «Folch», «Bligh and Dyer», and Soxhlet Extraction methods are detailed and explained. A special focus has been made for the complementary analytical methods such as HP-TLC (High...

This chapter presents the potential of primary and secondary metabolites produced by microorganisms after a brief introduction of their classification and detailing their cell wall structure. Furthermore, applications of microorganisms as sources of reagents and ingredients in various industries: food, cosmetic, pharmaceutics, nutraceutics, and bio...

This chapter reviews the recent development of extraction techniques, procedures and solvents for lipids from microorganisms. The modern innovative and intensified extraction techniques, alternative solvents and original procedures (ultrasound, microwave, supercritical fluid extraction, biobased-solvent, mechanical extraction, enzyme-assisted extra...

Vegetable oils take a large part in industry for food and non-food applications. However the extraction process of oil from oilseeds includes a solvent extraction step using hexane. Despite its various advantages it presents numerous drawbacks; it is sourced from petroleum, it has a high flammability and it appears to be dangerous for health and en...

Microorganisms called “oleaginous” such as yeast, bacteria, and microalgae, are increasingly studied as an alternative source of oils and fats owning high potential due to their productivity and rapid growth. Some species of oleaginous microorganisms are able to accumulate more than 20 % of their dry cell mass in the form of lipids. In a particular...

This review presents useful and green techniques of solvent-free extraction used in ancient times, such as extraction of olive oil and citrus essential oil, and innovative techniques, such as pulsed electric field, microwave, instantaneous controlled pressure drop, and extrusion. We discuss the devices, their applications, mechanisms, and parameter...

Extraction yields and fatty acid profiles from freeze-dried Chlorella vulgaris by microwave pretreatment followed by supercritical carbon dioxide (MW-SCCO(2)) extraction were compared with those obtained by supercritical carbon dioxide extraction alone (SCCO(2)). Work performed with pressure range of 20-28 Mpa and temperature interval of 40-70 °C,...

Currently, the most widely used solvent for extraction of vegetable oils is hexane given its various advantages such as ease of removal from the products, low boiling point and good lipid solubility. One of the major constituents of industrial hydrocarbons mixture is n-hexane; it is sourced from fossil resources and registered under the REACH Regul...

The present study was designed to evaluate the performance of alternative bio-based solvents, more especially 2-methyltetrahydrofuran, obtained from crop's byproducts for the substitution of petroleum solvents such as hexane in the extraction of fat and oils for food (edible oil) and non-food (bio fuel) applications. First a solvent selection as we...

In the past few years, much interest has focused on the applications of ultrasound in food processing and technology. Many reports suggest effective benefits from combining ultrasound with classical techniques. Applications of ultrasound generally involve processes that can enhance rates, improve quality and/or safety, and reduce processing time. U...

2-Methyltetrahydrofuran (MeTHF) is a solvent produced from renewable raw materials by the hydrogenation of products obtained from carbohydrate fractions of hemicellulose from various feedstocks. MeTHF has the advantages to be biodegradable and has a promising environmental footprint, good preliminary toxicology assessments, and an easy recycling. A...

One of the many environmental challenges faced by Extraction field is the widespread use of organic solvents. With a solvent based extraction the solvent necessarily has to be separated from the final extract. A large number of these solvents are toxic that pose a risk to workers and community members and virtually all of them are classified as vol...

This chapter presents a complete picture of current knowledge on useful and green bio-solvent “terpenes” obtained from aromatic plants and spices through a steam distillation procedure followed by a deterpenation process. Terpenes could be a successful substitute for petroleum solvents, such as dichloromethane, toluene, or hexane, for the extractio...