Maryam TabarzadShahid Beheshti University of Medical Sciences · Protein Technology Research Center
Maryam Tabarzad
PhD, Assistant Professor
About
59
Publications
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Introduction
Skills and Expertise
Publications
Publications (59)
Capsaicin is a bioactive phytochemical of red and chili peppers. It has shown therapeutic properties, including anticancer activities. In this study, the potential anti-telomerase effect of capsaicin, as well as synergic inhibitory effect of this compound in combination with cobalt ferrite-graphene oxide nanocomposites was investigated on breast ca...
Introduction
Insulin-like growth factor-1 (IGF-1) is a single-chain polypeptide with various physiological functions. Escherichia coli is one of the most desirable hosts for recombinant protein production, especially for human proteins whose post-translation modifications are not essential for their bioactivity, such as hIGF-1.
Objectives
In this...
Background: Mycosporine-like amino acids (MAAs) are ultraviolet-absorbing metabolites found in cyanobacteria, fungi, algae, and animals, known for their antioxidant and anti-inflammatory properties. Objectives: This study aimed to optimize MAAs extraction from Fischerella sp. F5 using experimental design techniques. Methods: The central composite d...
Natural products are the most accessible source of compounds in anticancer drugs, although there are several challenges in their application in the clinic. Phytosomes as phospholipids-based drug delivery carriers are novel to formulate poorly soluble phytoconstituents. Conversely, phytosomes can increase the lipophilicity of highly hydrophilic drug...
Heavy and irresponsible use of antibiotics in the last century has put selection pressure on the microbes to evolve even faster and develop more resilient strains. In the confrontation with such sometimes called “superbugs”, the search for new sources of biochemical antibiotics seems to have reached the limit. In the last two decades, bioactive ant...
Fischerella sp. is a valuable source of active metabolites, including UV-protecting compounds, among which mycosporin-like amino acids (MAAs) can be mentioned. Mycosporine-like amino acids are attractive secondary metabolites of a wide range of microorganisms, including microalgae and cyanobacteria. Enhanced production of MAAs has been studied in d...
Background
Spirulina is a cyanobacteria species containing various bioactive compounds. Spirulina is a known source of nutrients in some traditional diets. Different activities have been reported for various extracts of S. platensis.
Objectives
In this study, the polysaccharide content of culture media and biomass extract of one species of Spiruli...
Background and aims:
Due to the increasing incidence of skin cancer and other skin disorders caused by
ultraviolet (UV) irradiation, the development of more efficient sunscreens seems to be essential. Mycosporine-like amino acids (MAAs) are one group of natural UV-absorbent compounds with sunscreen characteristics that have been the focus of a numb...
Some antimicrobial peptides have been shown to be able to inhibit the proliferation of cancer cell lines. Various strategies for treating cancers with active peptides have been pursued. According to the reports, anticancer peptides are important therapeutic peptides, which can act through two distinct pathways: they either just create pores in the...
HIGHLIGHTS Spirulina platensis protein extract was digested with three types of protease. Digestion of protein extract by pepsin resulted in higher antioxidant activity. Digestion by a bacterial protease from Bacillus licheniformis resulted in a higher yield of peptide formation. ABSTRACT Keywords: Cyanobacteria Spirulina platensis Protein ex...
Due to their unique mechanisms of action, antimicrobial peptides (AMPs) are promising candidates to combat different infectious diseases. They usually non-specifically interact with the bacterial cell membrane, create pores in their membrane and increase its permeability which causes the death of pathogens. In the design and development of AMPs, in...
Background:
Cyanobacteria can produce compounds absorbing ultraviolet irradiation. Mycosporine like amino acids (MAAs) are some of these important metabolites, which can be potentially considered as a sunscreen agent in the pharmaceutical and cosmetic industry. Different factors have been reported that can affect the biosynthesis of MAA.
Objectiv...
Background
Current approaches used to overcome microbial infections are becoming inefficient due to the overuse or misuse of antibiotics. Antimicrobial peptides (AMPs) are one of the most promising substitutional candidates for commercial antibiotics.
Methods and Results
The publications in the field of in silico design of AMPs with focus on the w...
Todays, novel and/or resistant pathogenic micro-organisms emergence is a great challenge of the word, and the development of effective vaccines became a valuable preventive strategy against the threats of infectious diseases. New delivery systems can be an effective approach to improve available vaccines efficacy or to develop novel vaccines agains...
Aptamers can be regarded as efficient substitutes for monoclonal antibodies in many diagnostic and therapeutic applications. Due to the tedious and prohibitive nature of SELEX (systematic evolution of ligands by exponential enrichment), the in silico methods have been developed to improve the enrichment processes rate. However, the majority of thes...
Microalgae and cyanobacteria are the potentially valuable source of bioactive compounds applied in the various industries and human usage in different fields of pharmaceutical, nutraceutical, and cosmetic disciplines. One of the interesting aspects is their application as the anti-inflammatory agents for treatment of inflammation related mal-condit...
Mycosporine like amino acids (MAAs) are UV absorbing compounds which are produced by a variety of organisms such as algae and cyanobacteria, in order to protect themselves from harmful UV irradiation. Thus, they can potentially be used as sunscreens in pharmaceutical and cosmetic industry. Many abiotic factors are studied which induce the productio...
Feature extraction is one of the most important preprocessing steps in predicting the interactions between RNAs and proteins by applying machine learning approaches. Despite many efforts in this area, still, no suitable structural feature extraction tool has been designed. Therefore, an online toolbox, named RPINBASE which can be applied to differe...
Today, the treatment of bacterial infections is a major challenge, due to growing rate of multidrug-resistant bacteria, complication of treatment and increased healthcare costs. Moreover, new treatments for bacterial infections are limited. Oligonucleotide aptamers are single stranded DNAs or RNAs with target-selective high-affinity feature, which...
Silymarin is a complex extract isolated from the plant Silybum marianum, widely known for its prominent antioxidant and hepatoprotective effects, although increasing evidences have reported extraordinary antiproliferative and apoptotic abilities. As a result, several signaling pathways involved in cell cycle control, cell proliferation, and cell de...
Oxidation of a vast range of phenolic and non-phenolic substrates has been catalyzed by laccases. Given wide range of substrates, laccases can be applied in different biotechnological applications. The present review was conducted to provide a broad context in pharmaceutical- and biomedical-related applications of laccases for academic and industri...
Blinatumomab, the bispecific T cell engager antibody (BsAb), has been demonstrated as the most successful BsAb to date. Throughout the past decade, vector design has great importance for the expression of monoclonal antibody in Chinese hamster ovary (CHO) cells. It has been indicated that expression vectors based on the elongation factor-1 alpha (E...
Recently, antimicrobial peptides have been introduced as potent antibiotics with a wide range of antimicrobial activities. They have also exhibited other biological activities, including anti-inflammatory, growth stimulating, and anti-cancer activities. In this study, an analog of Magainin II was designed and produced as a recombinant fusion protei...
Conventional treatment for cancer such as surgical resection and chemotherapy can cause damage in cases with advanced cancers. Moreover, the identification of tumor-specific targets has great importance in T-cell therapies. For decades, T cell activity has been stimulated to improve anti-tumor activity. Bispecific antibodies have attracted strong i...
Recently, much attention has been focused on the use of miRNAs in cancer treatment. The role of proto-oncogene Janus kinase-2 (JAK-2) in proliferation and survival of gastric cancer has been previously documented. The aim of this study was to evaluate the effect of a chimera consisted of nucleolin specific aptamer (NCL-Apt) and miRNA let-7d on JAK2...
The "bispecifics" market improved over the past decade due to the development of many technological platforms including bispecific T cell engagers (BiTEs). The approval of blinatumomab, the most advanced bispecific T-cell engager (BiTE) in clinical trials, can be a significant milestone in the development of bispecific antibodies. Both Chinese hams...
IGF-I as a human growth factor produced in Escherichia Coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7.6 kDa. Up to now, E. coli expression system has been widely used as a host to produce rhIGF-1 with high yields. Acyl homoserine lactones (AHLs) are intercellular signaling molecules...
Abstract: Background: IGF-I as a human growth factor produced in Escherichia Coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7.6
kDa. Up to now, E. coli expression system has been widely used as the host to produce rhIGF-1 with high yields. Acyl Homoserine Lactones (AHLs) are intercellu...
Janus Kinase (JAK) family is a group of four signaling enzymes composing of four distinct domains and involved in the intracellular pathways of cytokine downstream signaling. There are two kinase domains at C-terminal of protein, one of which is regulatory and the other has the main functionality in phosphorylation of target proteins. JAKs involve...
Peptide and protein based therapeutics are the most promising approaches in today medicine. Bioactive peptides can be valuable drugs in the treatment of various illnesses, such as cardiovascular and neurodegenerative diseases. Cell toxic peptides can be considered for cancer or infection therapy. Antimicrobial peptides (AMPs) are one of the most in...
miRNAs as one of the potential therapeutic agents have been recently considered for cancer treatment. AS1411 (aptNCL) is a DNA aptamer specifically binding to nucleolin protein on the cancer cell surface with antiproliferative effect. The aim of the study was to develop a conjugate consisting of aptNCL (as targeted delivery of therapeutic agent) an...
Introduction:
Magnetic nanoparticles (MNPs) are one of the most useful particulate systems in analytical applications such as specific aptamer selection. Proteins are the most noted targets of aptamer selection. Generally, covalently immobilized protein coated MNPs are more stable structures.
Methods:
In this study, coagulation factor VIII (FVII...
Casein phosphopeptides (CPPs) are mineral-binding bioactive peptides derived from enzymatic digestion of casein. They have been shown to increase iron bioavailability due to increasing iron solubility at the alkaline pH of the intestine. Thus, they are potentially applicable as an advantageous preparation for iron fortification of food. This study...
Cancer stem cells (CSCs) are one of the most important origins of cancer progression and metastasis. CSCs have unique self-renewal properties and diverse cell membrane receptors that induced the resistance to the conventional chemotherapeutic agents. Therefore, the therapeutic removal of CSCs could result in the cancer cure with lack of recurrence...
Combination of aptamers with DNAzymes attracted intense attention for development of DNA-based biosensors for detection of mycotoxins. In the present study a combination of aflatoxin B1 specific aptamer and HRP- (horseradish peroxidase-) mimicking DNAzyme was optimized for detecting aflatoxin B1. Detecting approach is based on the binding affinity...
Polymeric drug delivery systems in the form of nanocarriers are the most interesting vehicles in anti-cancer therapy. Among different types of biocompatible polymers, carbohydrate-based polymers or polysaccharides are the most common natural polymers with complex structures consisting of long chains of monosaccharide or disaccharide units bound by...
Background
Among diverse protein purification systems, affinity chromatography is the most attractive one in the purification process of coagulation factors. Coagulation factor VII is a plasma serine protease that has a significant role in natural human hemostasis and its recombinant form such as AryoSeven™, has been applied in clinical treatment o...
Protein specific aptamers are highly applicable affinity ligands in different fields of research and clinical applications. They have been developed against various targets, in particular, biomacromolecules such as proteins. Among human proteins, the coagulation factors are the most attractive targets for aptamer selection and their specific aptame...
DNAzyme and aptamer conjugations have already been used for sensitive and accurate detection of several molecules. In this study, we tested the relationship between conjugation orientation of DNAzyme and Aflatoxin B1 aptamer and their subsequent peroxidase activity. Circular dichroism (CD) spectroscopy and biochemical analysis were used here to dif...
In the pharmaceutical industry, hard- and soft-shelled capsules are typically made from gelatin, commonly derived from bovine and porcine sources. To ensure that pharmaceutical products comply with halal regulations in Muslim countries (no porcine products allowed), development of a valid, reliable, quick, and most importantly, cost-effective tests...
The development of enhanced drug delivery systems is one of the most attractive fields of pharmaceutical sciences, as some of the highly effective chemo/biotherapeutics for cancer treatment can not be administrated due to their high toxicities for normal cells or low stability in physiological media. However, drugs that are currently not administra...
Aptamers are single stranded oligonucleotides, comparable to monoclonal antibodies (mAbs) in selectivity and affinity and have significant strategic properties in design, development and applications more than mAbs. Ease of design and development, simple chemical modification and the attachment of functional groups, easily handling and more adaptab...
L-Asparaginase has remarkable properties which make it useful in dual pharmaceutical and food industries.In this study, simple and advantageous methods have been validated for rapid and precise determination of intracellular L-Asparaginasein bacterial species. A suspension of bacterial cells was used instead of cell extract and incubated by substra...
Aptamers, or single stranded oligonucleotides, are produced by systematic evolution of ligands by exponential enrichment, abbreviated as SELEX. In the amplification and regeneration step of SELEX technique, dsDNA is conversed to ssDNA. Asymmetric PCR is one of the methods used for the generation of ssDNA. The purpose of this study was to design a r...
Voltage-dependent calcium channels are crucial targets for a wide range of clinically active pharmacological agents. From these agents, 1,4-dihydropyridines constitute a group of small organic compounds are based on a core pyridine structure which can both block and enhance calcium currents. They are considered specific for L-Type calcium channels;...
The Carbonic Anhydrase (CA) inhibitory activities of a large number of sulfonamide derivatives toward CA isoenzyme types I, II, IV, and IX were collected and then subjected to QSAR analyses. The dataset used consisted of 343 sulfonamide derivatives with 200 calculated descriptors. For each type of isoenzyme, a separate QSAR model was obtained using...
The search for novel antibacterial agents active against Mycobacterium tuberculosis and other atypical Mycobacterium is urgent due to the lack of effectiveness of known anti-tuberculosis agents against opportunistic pathogens as a consequence of rapidly emerging resistance. In this study, QSAR analysis was conducted on the anti-tuberculosis activit...
A quantitative structure activity relationship analysis has been applied to a data set of 20 N-phenyl-N′-(4-pyridinyl)-urea derivatives with anticonvulsant activity. Semi-empirical quantum chemical calculations at AM1 level was used to find the optimum 3D geometry of the studied molecules. Four types of molecular descriptors including classical (su...