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Publications (4)
Dracocephalum L. is a well-known aromatic and medicinal plant genus in the Mint family. Some species of the genus such as D. kotschyi (DK) are widely used in Iranian folk medicine as tonic and carminative, as well as for relieves of headache, stomachache, and liver ailments. The present study was aimed to: isolate and determine some bioactive flavo...
Over-consumption of antibiotics has led to antimicrobial resistance creating urgency to find fast and efficient techniques to monitor the level of antibiotics in the foods. The advantages of using aptamer for a variety of scientific challenges are becoming more abundant in different fields, making its applications more effective and cost-efficient....
The major bioactive compounds of saffron flowers are picrocrocin, and safranal, which significantly contribute towards the quality of saffron. Nevertheless, the variation in the metabolite profiling among Iranian saffron accessions with different origins has been reported due to the uniformity of cultivation, climatic conditions, and agronomic prac...
Saffron (Crocus sativus L.) is a precious commodity with a high economic value for exporting countries such Iran. One of the major challenges of saffron cultivation is the lack of a reliable method of saffron secondary metabolites determination. This study is aimed to introduce a reliable and accurate method for determining the quality of saffron....
Questions
Questions (2)
Hello everyone,
I did a TA cloning. My DNA was 70 bp and the vector was PTZ57R/T. To check the insertion I did PCR by Plasmids extracted from white colonies and M13 (as primers). As result, I found many PCR products that are small (approximately 150 bP), even smaller than blue colonies. On the other hand, I have not any bond in control PCR. It means these very small bonds are not dimer primer. I am wondering how I can interpret this result.
I red some articles that mentioned “large scale PCR” but for this much pool, there is need for at least 3300, (50 µL) PCR reactions. But it does not make sense to me!! Because after this PCR process, desalting should be done for this much of pool.
