Martina G Vijver

Leiden University | LEI

Herein, we investigated to which extent metallic nanoparticles (MNPs) affect the trophic transfer of other coexisting MNPs from lettuce to terrestrial snails and the associated tissue-specific distribution using toxicokinetic (TK) modeling and single-particle inductively coupled plasma mass spectrometry. During a period of 22 days, snails were fed...

The wide production and use of metallic nanomaterials (MNMs) leads to increased emissions into the aquatic environments and induces high potential risks. Experimentally evaluating the (eco)toxicity of MNMs is time-consuming and expensive due to the multiple environmental factors, the complexity of material properties, and the species diversity. Mac...

Over the last years there has been significant research on the presence and effects of plastics in terrestrial systems. Here we summarize current research findings on the effects of nano- and microplastics (NMPs) on terrestrial plants, with the aim to determine patterns of response and sensitive endpoints. We conducted a systematic review (based on...

Material constraints may slow the pace of energy transition if the materials intensity of renewable energy technologies remains the same. Innovations in solar photovoltaics (PV) can contribute to achieving lower material demands. In this research, the actor-centered institutionalism framework, transitions literature and the science-policy interface...

Ongoing efforts focus on quantifying plastic pollution and describing and estimating the related magnitude of exposure and impacts on human and environmental health. Data gathered during such work usually follows a receptor perspective. However, Life Cycle Assessment (LCA) represents an emitter perspective. This study examines existing data gatheri...

Chemicals emitted to the environment affect ecosystem health from local to global scale, and reducing chemical impacts has become an important element of European and global sustainability efforts. The present work advances ecotoxicity characterization of chemicals in life cycle impact assessment by proposing recommendations resulting from internat...

The rapid development of nanomaterials (NMs) and the emergence of new multicomponent NMs will inevitably lead to simultaneous exposure of organisms to multiple engineered nanoparticles (ENPs) at varying exposure levels. Understanding the joint impacts of multiple ENPs and predicting the toxicity of mixtures of ENPs are therefore evidently of import...

New synthetic chemicals and materials are rising rapidly and are already widespread as novel entities in our daily lives. Although knowledge of their disruptive and long-lasting effects on the Earth system is accumulating, unknowns remain. This Voices asks: what risks do novel entities pose, and what are the emerging concerns?

The Safe by Design (SbD) concept aims to ensure the production, use and disposal of materials and products safely. While there is a growing interest in the potential of SbD to support policy commitments, such as the EU Green Deal and the Circular Economy Action Plan in Europe, methodological approaches and practical guidelines on SbD are, however,...

The goal of the current study was to quantify the trophic transfer of copper nanoparticles (CuNPs) in a food chain consisting of the microalga Pseudokirchneriella subcapitata as the representative of primary producer, the grazer Daphnia magna, and the omnivorous mysid Limnomysis benedeni. To quantify the size and number concentration of CuNPs in th...

Soil extra-cellular enzymes are the main driving force for microbial and biochemical processes, which makes them sensitive indicators for soil health and quality. Returning large amounts of sludge or its biochar to farmland may introduce exogenous substances into soil and have a significant impact on soil enzymatic activity. This study aimed to eva...

Multigenerational toxicity tests provide more sensitive measures of population-level effects than conventional single-generation tests. Particularly for stressors which exhibit slow uptake rates (e.g. nanomaterials), multigenerational tests may also provide a more realistic representation of natural exposure scenarios. To date, the inherently high...

Engineered nanomaterials (ENMs) are ubiquitous nowadays, finding their application in different fields of technology and various consumer products. Virtually any chemical can be manipulated at the nano-scale to display unique characteristics which makes them appealing over larger sized materials. As the production and development of ENMs have incre...

Ingested nanomaterials are exposed to many metabolites that are produced, modified, or regulated by members of the enteric microbiota. The adsorption of these metabolites potentially affects the identity, fate, and biodistribution of nanomaterials passing the gastrointestinal tract. Here, we explore these interactions using in silico methods, focus...

To assess the safety of engineered nanomaterials (ENMs) and to evaluate and improve ENMs' targeting ability for medical application, it is necessary to analyze the fate of these materials in biological media. This protocol presents a workflow that allows researchers to determine, characterize and quantify metal-bearing ENMs (M-ENMs) in biological t...

Many host-microbiota interactions depend on the recognition of microbial constituents by toll-like receptors of the host. The impacts of these interactions on host health can shape the hosts response to environmental pollutants such as nanomaterials. Here, we assess the role of toll-like receptor 2 (TLR2) signaling in the protective effects of colo...

Recently, the delivery of pesticides through novel controlled-release (nano-)formulations has been proposed intending to reduce (incidental) pesticide translocation to non-target sites. Concerns have however been raised with regards to the potentially enhanced toxicity of controlled-release (nano-)formulations to non-target organisms and ecosystems...

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes the coronavirus disease-19 (COVID-19) pandemic spread across the world and remains difficult to control. Environmental pollution and habitat conditions do facilitate SARS-CoV-2 transmission as well as increase the risk of exposure to SARS-CoV-2. The coexistence of microplastics...

Screening and prioritization of chemicals is essential to ensure that available evaluation capacity is invested in those substances that are of highest concern. We, therefore, recently developed structural similarity models that evaluate the structural similarity of substances with unknown properties to known Substances of Very High Concern (SVHC),...

The European Green Deal outlines ambitions to build a more sustainable, climate neutral, and circular economy by 2050. To achieve this, the European Commission has published the Chemicals Strategy for Sustainability: Towards a Toxic-Free Environment, which provides targets for innovation to better protect human and environmental health, including c...

Regulation of plastics has emerged as a significant science-policy challenge, initiated by the increasing societal concerns regarding plastic pollution. A specific focus is now on plastic of sizes smaller than 1000 nm, often referred to as nanoplastics. The need to include nanoplastics in existing regulatory frameworks arises from the increased bio...

The conventional Hill equation model is suitable to fit dose‐response data obtained from performing (eco)toxicity assays. Models based on quasi‐QSARs to estimate the Hill coefficient ( n H ) were developed with the aim of predicting the response of the invertebrate species Daphnia magna to exposure to metal‐based nanomaterials. Descriptors represen...

In recent years, various ecotoxicological test guidelines and (technical) guidance documents have been evaluated and updated with regard to their applicability to nanomaterials (NMs). Several of these have currently reached official regulatory status. Ensuring their harmonized implementation with previously recognized methods for ecotoxicity testin...

Similarity assessment is one of the means of optimally using scarcely available experimental data on the fate and hazards of nanoforms (NFs) for regulatory purposes. For a set of NFs that are shown to be similar it is allowed in a regulatory context to apply the information available on any of the NFs within the group to the whole set of NFs. Obvio...

Plastics in the environment can be degraded to nanoscale plastic debris (NPD) with a size smaller than 1 µm. But it is unknown how the physicochemical properties of NPD influence their interaction with organisms and their toxicokinetics in organisms' bodies. This study uses the knowledge gained from investigating engineered polymeric nanomaterials...

Microplastics in the environment are a serious environmental problem. The absence of standard methods for sampling and sample preparation and techniques for characterization and quantification of microplastics, particularly, in the complex matrices of environmental samples hinder assessing the fate and the risk of these emerging contaminants. In th...

Microplastics in the environment are a serious environmental problem. The absence of standard methods for sampling and sample preparation and techniques for characterization and quantification of microplastics, particularly, in the complex matrices of environmental samples hinder assessing the fate and the risk of these emerging contaminants. In th...

The meaning of the term ‘life cycle’ in relation to chemical, material and product in Safe-by-Design (SbD) studies is discussed. We recommend including explicit definitions and graphical representations of life cycles in future SbD work.

The current debate on hazards associated with sub-micron sized plastics is hampered by a lack of quantitative data on the uptake and biological fate of plastics in organisms. Analytical methods should be developed to identify, characterize, and quantify sub-micron particulate plastic in biota to understand their biological fate in terms of biodistr...

The increasing application of biosolids and agrochemicals containing silver nanoparticles (AgNPs) and titanium dioxide nanoparticles (TiO2NPs) results in their inevitable accumulation in soil, with unknown implications along terrestrial food chains. Here, the trophic transfer of single NPs and a mixture of AgNPs and TiO2NPs from lettuce to snails a...

Assessment of chronic impact of metallic nanoparticles (NPs) in soil ecosystems is a necessity for ensuring safe and sustainable application. NPs affect plants and their associated microbial life, while the plants and their associated microbiota affect the NPs’ fate. Here, we measured the available Ag pool (determined as diethylenetriaminepentaacet...

Significance Survey data show a large-scale decline in insects. This global decline is often linked to human actions in intensive agricultural areas. To investigate whether this decline has a causal relationship with neonicotinoid insecticides, we performed an outdoor experiment with representative surface water concentrations of the neonicotinoid...

The rapid development of nanotechnology influences the developments within the agro-sector. An example is provided by the production of nanoenabled pesticides with the intention to optimize the efficiency of the pesticides. At the same time, it is important to collect information on the unintended and unwanted adverse effects of emerging nanopestic...

Various modern products have metallic nanoparticles (MNPs) embedded to enhance products performance. Technological advances enable nowadays even multiple hybrid nanoparticles. Consequently, the future co-release of multiple MNPs will inevitably result in the presence of MNP mixtures in the environment. An important question is if the responses of m...

Copper oxide nanoparticles (CuO NPs) are one of the most widely used materials owing to their excellent properties such as thermal and photochemical stability, superconductivity, and high electrochemical activity. Once they enter the environment, Cu²⁺ may be released in water, which alters the behavior and toxicity of CuO NPs. The present study thu...

The Association of nanoparticles (NPs) with algae likely plays a critical role in their transfer in aquatic food chains. Although our understanding of the ecotoxicity and fate of NPs in the environment is increasing, it is still unclear how the physicochemical properties of NPs influence their interaction with algae at cellular levels and how this...

The coronavirus disease-19 (COVID-19) pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is rampant in the world and is a serious threat to global health. The SARS-CoV-2 RNA has been detected in various environmental media, which speeds up the pace of the virus becoming a global biological pollutant. Because many en...

A QSAR model was developed to predict the response (immobilization) of Daphnia magna following exposure to metal and metal oxide nanomaterials.

Nanomaterials (NMs) taken up from the environment carry a complex ecocorona consisting of dissolved organic matter. An ecocorona is assumed to influence the interactions between NMs and endogenous biomolecules and consequently affects the formation of a biological corona (biocorona) and the biological fate of the NMs. This study shows that biomolec...

Sample preparation for extraction of nanoscale plastic debris (NPD, size < 1 µm) from environmental samples is a critical step to prepare NPD for further identification and quantification. Developing an NPD extraction method from soil matrices is, particularly, challenging due to the complexity of solid matrices. In the present study, we built upon...

The impacts of humic substances (HS) on the aquatic stability and toxicity of nano-cerium dioxide (nCeO2) to three organisms with different exposure characteristics were investigated. Addition of HS to suspensions of nCeO2 lowered the surface zeta potential of the particles, reduced their hydrodynamic size, and increased the energy barrier as indic...

Analytical limitations considerably hinder our understanding of the impacts of the physicochemical properties of nanomaterials (NMs) on their biological fate in organisms. Here, using a fit-for-purpose analytical workflow, including dosing and emerging analytical techniques, NMs present in organisms are characterized and quantified across an aquatic f...

Due to the large amount of chemical substances on the market, fast and reproducible screening is essential to prioritize chemicals for further evaluation according to highest concern. We here evaluate the performance of structural similarity models that are developed to identify potential substances of very high concern (SVHC) based on structural s...

Titanium dioxide nanoparticles (TiO2 NPs) are widely used as nano-agrochemicals. In this study we investigated the influence of soil heterogeneity on bacterial communities exposed to TiO2 NPs over time. Clay and sandy soils with low- and high-organic matter contents were exposed to environmentally relevant concentration of TiO2 NPs (1 mg/kg) and so...

Novel products often have a multitude of nanomaterials embedded; likewise within many products graphite-based products are decorated with nano-zirconium oxide (nZrO2) because graphene is an ultrahigh conductive material whereas nZrO2 is for instance fire-retardant. As a consequence, the pristine/isolated nanoparticle has unique beneficial propertie...

Teleost fish embryos are protected by two acellular membranes against particulate pollutants that are present in the water column. These membranes provide an effective barrier preventing particle uptake. In this study, we tested the hypothesis that the adsorption of antimicrobial titanium dioxide nanoparticles onto zebrafish eggs nevertheless harms...

With the expanding nanotechnology, nanoparticles (NPs) embedded products are used in the agricultural sector to improve soil fertility. Thus, two typical metal oxides NPs and their mixtures were applied in different doses to evaluate the impacts on soil microbes. CuO and ZnO NPs boosted soil microbial communities as reflected by the increased numbe...

The existing limitations in analytical techniques for characterization and quantification of nanoscale plastic debris (NPD) in organisms hinder understanding of the parental and trophic transfer of NPD in organisms. Herein, we used iron oxide-doped polystyrene (PS) NPD (Fe-PS-NPD) of 270 nm and Europium (Eu)-doped PS-NPD (Eu-PS-NPD) of 640 nm to ci...

Innovation in photovoltaics (PV) is mostly driven by the cost per kilowatt ratio, making it easy to overlook environmental impacts of technological enhancements during early research and development stages. As PV technology developers introduce novel materials and manufacturing methods, the well‐studied environmental profile of conventional silicon...

Functionalized high-aspect-ratio silver nanowires (AgNWs) have been recognized as one of the most promising alternatives for fabricating products, with their use ranging from electronic devices to biomedical fields. Given concerns on the safety of AgNWs, there is an urgent need to investigate the relation between intrinsic properties of AgNWs and t...

Multijunction III–V/silicon photovoltaic cells (III–V/Si), which have achieved record conversion efficiencies, are now looking as a promising option to replace conventional silicon cells in future PV markets. As efforts to increase efficiency and reduce cost are gaining important traction, it is of equal importance to understand whether the manufac...

An approach to solve the emerging need of prediction of the toxicity of mixtures of engineered nanoparticles (ENPs) is presented. The integration of classic approaches to mixture toxicity assessment and computational toxicology approaches is proposed to be a smart strategy for forecasting the toxicity of a mixture of ENPs.

There is a debate on whether the Trojan horse principle is occurring for nanoscale plastic debris (NPD < 1 µm). It is realized that NPD have a high capacity to sorb environmental contaminants such as metals from the surrounding environment compared to their microplastic counterparts, which influences the sorbed contaminants’ uptake. Herein, we stud...

There is a debate on whether the Trojan horse principle is occurring for nanoscale plastic debris (NPD < 1 µm). It is realized that NPD have a high capacity to sorb environmental contaminants such as metals from the surrounding environment compared to their microplastic counterparts, which influences the sorbed contaminants’ uptake. Herein, we stud...

PurposeThe goal of this study is to perform an ex-ante life cycle assessment (LCA) of the emerging gallium-arsenide nanowire tandem solar cells on silicon (GaAs/Si) and to provide a benchmark for the commercialization of the technology. The environmental impacts and energy payback time (EPBT) of the GaAs/Si modules are compared with those of the in...

Nanotechnology can potentially revolutionize the agricultural industry by offering nano-formulations of pesticides, so-called nano-pesticides which can e.g. increase the efficacy and stability of the active ingredients of pesticides. However, it is unknown how the nano-formulation may modulate the interaction between the active ingredient and non-t...

Global production of fibrous material is significantly growing with expectation of reaching 145 million metric tons in2030. Production includes mostly synthetic polymers fibers, cotton fibers and man-made cellulosic (viscose) fibers. Asmaller contribution comes from animal-made fibers (wool, silk). The main uses of fibrous material are in clothing,...

It is increasingly recognized that the ubiquity of convenient single-use plastic has resulted in a global plastic pollution challenge, with substantial environmental and health consequences. Physical, chemical, and biological processes result in plastic weathering, with eventual formation of debris in the micro to nano size range. There is an incre...

Metal-based nanoparticles exhibiting antimicrobial activity are of emerging concern to human and environmental health. In addition to their direct adverse effects to plants and animals, indirect effects resulting from disruption of beneficial host–microbiota interactions may contribute to the toxicity of these particles. To explore this hypothesis,...

Biodegradable chelators (BCs) are promising substitutes for conventional washing agents in the remediation of heavy metal contaminated soil with strong complexing ability and less cost. However, great challenges for the applications of BC–assisted washing still exist, such as the assessment of the factor affecting the efficiency of metal removal an...

A key source of uncertainty in the environmental assessment of emerging technologies is the unpredictable manufacturing, use, and end-of-life pathways a technology can take as it progresses from lab to industrial scale. This uncertainty has sometimes been addressed in life cycle assessment (LCA) by performing scenario analysis. However, the scenari...

Pb-based perovskite nanoparticles (PbPNPs) are amongst others used within highly efficient solar cells. PbPNPs can be released into the environment during their production, recycling or waste processing. In this study we investigated the fate and toxicity of PbPNPs on soil bacterial community under simulated natural environmental conditions across...

Whether toxicity of silver nanoparticles (AgNPs) to organisms originates from the nanoparticles themselves or from the dissolved Ag-ions is still debated, with the majority of studies claiming that extracellular release of Ag-ions is the main cause of toxicity. The objective of this study was to determine the contributions of both particles and dis...

Nanocellulose is a functional material derived from natural carbon-based polymers. These nanomaterials are biodegradable and renewable in nature and hence are seen as environmentally-friendly materials in many applications. The use of such innovative materials is accelerating and inescapable there is a need to test these presumed environmentally-fr...

Transformation of nutrients to their nano-form, such as selenium (Se) engineered nanonutrients (Se-ENNs), is expected to enhance the absorption of the nutrients into fish and increase the efficiency of the feed. However, dissolution, aggregation, and release of ENNs from the feed matrix may decrease the efficiency of the Se-ENNs. In this study, we...

Humic substances are the dominant dissolved organic matter fraction in the aqueous phase of environmental media. They would inevitably react with chemicals released into the environment. The influence of dissolved humic acid (DHA) on the dissolution and dispersion of copper oxide nanoparticles (CuO NPs, 50 nm, 49.57 mg L-1) was therefore investigat...

A single-cell inductively coupled plasma mass spectrometry technique was used to explore the influence of particle properties on the association of nanomaterials (NMs) with algal cells. We investigated the effect of particle size, shape and surface chemistry [citrate and natural organic matter (NOM) coating] on the association of gold (Au)-NMs with...

We systematically investigated how the combinations of size, shape and the natural organic matter (NOM)-ecocorona of gold (Au) engineered nanoparticles (ENPs) influence the attachment of the particles to algae and physical toxicity to the cells. Spherical (10, 60 and 100 nm), urchin-shaped (60 nm), rod-shaped (10 × 45, 40 × 60 and 50 × 100 nm), and...