Martina RadićUniversity of Bern | UniBe · Department for BioMedical Research
Martina Radić
PhD
Postdoctoral Researcher
About
19
Publications
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Introduction
Publications
Publications (19)
Understanding the spatial heterogeneity of tumours and its links to disease initiation and progression is a cornerstone of cancer biology. Presently, histopathology workflows heavily rely on hematoxylin and eosin and serial immunohistochemistry staining, a cumbersome, tissue-exhaustive process that results in non-aligned tissue images. We propose t...
Recent research suggests the potential categorization of bladder cancer (BLCa) patients into molecular subtypes to aid in the selection of appropriate treatment strategies, be it conventional, targeted, or a combination thereof. However, the practical implementation of molecular classifiers has faced obstacles due to the intricate nature of gene re...
Understanding the spatial heterogeneity of tumors and its links to disease initiation and progression is a cornerstone of cancer biology. Presently, histopathology workflows heavily rely on hematoxylin & eosin (H&E) and serial immunohistochemistry (IHC) staining, a cumbersome, tissue-exhaustive process that results in non-aligned tissue images. We...
Understanding the spatial heterogeneity of tumors and its links to disease is a cornerstone of cancer biology. Emerging spatial technologies offer unprecedented capabilities towards this goal, but several limitations hinder their clinical adoption. To date, histopathology workflows still heavily depend on hematoxylin & eosin (H&E) and serial immuno...
Background
In intermediate-risk non-muscle invasive bladder cancer (NMIBC) clinical guidelines suggest an adjuvant instillation with a chemotherapeutic agent. However, the agent and regimen are not clearly defined. Worldwide, less than 15% of patients receive this adjuvant chemotherapeutic instillation. We recently developed a pipeline for the gene...
The determination of the protein’s intracellular localization is essential for understanding its biological function. Protein localization studies are mainly performed on primary and secondary vertebrate cell lines for which most protocols have been optimized. In spite of experimental difficulties, studies on invertebrate cells, including basal Met...
Background: In intermediate risk non-muscle-invasive bladder cancer (NMIBC) clinical guidelines suggest an adjuvant instillation with a chemotherapeutic agent. However, the agent and regimen are not clearly defined. Worldwide, less than 15% of patients receive this adjuvant chemotherapeutic instillation. We recently developed a pipeline for the gen...
Regardless of the significant improvements in treatment of melanoma, the majority of patients develop resistance whose mechanisms are still not completely understood. Hence, we generated and characterized two melanoma-derived cell lines, primary WM793B and metastatic A375M, with acquired resistance to the RAF inhibitor vemurafenib. The morphology o...
Background
NME6 is a member of the nucleoside diphosphate kinase (NDPK/NME/Nm23) family which has key roles in nucleotide homeostasis, signal transduction, membrane remodeling and metastasis suppression. The well-studied NME1-NME4 proteins are hexameric and catalyze, via a phospho-histidine intermediate, the transfer of the terminal phosphate from...
Cutaneous melanoma is the most aggressive form of skin cancer. Despite the significant advances in the management of melanoma in recent decades, it still represents a challenge for clinicians. The TP53 gene, the guardian of the genome, which is altered in more than 50% of human cancers, is rarely mutated in melanoma. More recently, researchers star...
Nucleoside diphosphate kinases (NDPK/NME/Nm23) are enzymes composed of subunits NME1/NDPK A and NME2/NDPK B, responsible for the maintenance of the cellular (d)NTP pool and involved in other cellular processes, such as metastasis suppression and DNA damage repair. Although eukaryotic NDPKs are active only as hexamers, it is unclear whether other NM...
Unlike other tumours, TP53 is rarely mutated in melanoma; however, it fails to function as a tumour suppressor. We assume that its functions might be altered through interactions with several families of proteins, including p53/p73, NME and GLI. To elucidate the potential interplay among these families we analysed the expression profiles of aforeme...
Introduction
Sphingosine-1-phosphate (S1P), a potent signalling lipid. It mediates its actions by binding to a family of G-protein coupled receptors (GPCRs), known as S1P receptors. It has been implicated in the several processes integral to carcinogenesis. S1P signalling promotes cancer by inhibiting apoptosis and by enhancing proliferation, trans...
Introduction
TP53 is the most frequently mutated gene in human cancer. However, in metastatic melanoma mutations of TP53 occur infrequently and p53 fails to function as a tumour suppressor. The altered expression of p53 family members, including p53/p73 isoforms, as well as of the interactions among them could affect normal function of p53. Further...
Introduction
Malignant melanoma is the most aggressive form of skin cancer and resistant to available therapies, therefore new molecular approaches for better understanding of disease are needed. Although TP53 is rarely mutated in melanoma, it fails to function as a tumour suppressor. This may result from alterations in p53 family members, includin...
Liquid chromatography coupled to electrospray ionization mass spectrometry is routinely used in proteomics research. Mass spectrometry-based peptide analysis is de facto performed in positive ion mode, except for the analysis of some post-translationally modified peptides (e.g. phosphorylation and glycosylation). Collected mass spectrometry data af...
Questions
Question (1)
I'm doing research on protein (A and B isoform) that form enzymatically active hexameric complex. These hexamers are represented in all combinations (A6, A5B,..., B6). I would like to find out if they are all equally represented in my cell line or some hexamers are preferred? I can do immunoprecipitation and after that I tried native electrophoresis (without SDS) and western blot, but didn't get any results. Do you have any idea what should I do after ip in order to separate and distinguish these hexamers?
