Martin ModerCeMM Research Center for Molecular Medicine | CeMM
Martin Moder
PhD
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14
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Citations since 2017
Introduction
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Publications
Publications (14)
Defects in DNA repair can cause various genetic diseases with severe pathological phenotypes. Fanconi anemia (FA) is a rare disease characterized by bone marrow failure, developmental abnormalities, and increased cancer risk that is caused by defective repair of DNA interstrand crosslinks (ICLs). Here, we identify the deubiquitylating enzyme USP48...
Maintenance of genome integrity via repair of DNA damage is a key biological process required to suppress diseases, including Fanconi anemia (FA). We generated loss-of-function human haploid cells for FA complementation group C (FANCC), a gene encoding a component of the FA core complex, and used genome-wide CRISPR libraries as well as insertional...
Maintenance of genome integrity via repair of DNA damage is a key biological process required to suppress diseases, including Fanconi anemia (FA). We generated loss-of-function human haploid cells for FA complementation group C (FANCC), a gene encoding a component of the FA core complex, and used genome-wide CRISPR libraries as well as insertional...
Proper development of the immune system is an intricate process dependent on many factors, including an intact DNA damage response. The DNA double-strand break signaling kinase ATM and its cofactor NBS1 are required during T cell development and for the maintenance of genomic stability. The role of a second ATM cofactor, ATMIN (also known as ASCIZ)...
Questions
Question (1)
Hi fellow researchers!
I have some problems with the separation of two fragments on an arose gel. It would be nice if somebody could share some tips with me.
I want to digest a vector to cut out a small fragment. I have to cut with two different enzymes, the backbone is 7500 bp, whereas the backbone after insert removal is 7000 bp. So I need a good separation on the gel to separate and elute the digested vector. The problem is that I get a weird kind of smear, that I’ve never seen before. That makes it impossible for me to separate the fragments properly.
Does anybody know where this could come from? Here are my digerstion conditions:
1 yl vector (=1303ng)
2 yl buffer Cut smart
0,5 yl NheI
0,5 yl XhoI
16 yl dH2O
___________
20 yl
I load it together with 6x dye onto a 1% Agarose gel and run it with 100V for 4h. The digestion itself works, but the separation fails.
The very left band is an undigested control, in wich only one enzyme was used. The smear hinders me from separating the bands.
Any ideas?
Thanks guys!
Martin
