Martin Hessling

Martin Hessling
Technische Hochschule Ulm · Mechatronics and Medical Engineering - Biotech Lab

Dr.

About

131
Publications
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Introduction
We are interested in joint research projects, especially in the following fields: Medical Engineering, Biotechnology, Biophotonics, Bioreactors/Optical Bioreactor Sensors/Probes, UV-Disinfection, UV-LEDs, LED-Illumination in Medical Applications Current research projects are: -Clean Spring; Biofilm/pathogen reduction in drinking water systems -Safe Light: intraocular illumination for vitreoretinal surgery -Biooptiss: Bioreactor for cartilage tissue engineering with special optical analytics

Publications

Publications (131)
Article
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Background Due to the increase in multidrug-resistant pathogens, it is important to investigate further antimicrobial options. In order not to have to work directly with pathogens, the investigation of possible surrogates is an important aspect. It is examined how suitable possible surrogate candidates for ESKAPE pathogens are for UVC applications....
Article
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For SARS-CoV-2 disinfection systems or applications that are based on UVC, UVB or UVA irradiation, it would be desirable to have a SARS-CoV-2 surrogate for tests and development, which does not require a laboratory with a high biosafety level. The bacteriophage Phi 6, an enveloped RNA virus like coronaviruses, is an obvious candidate for such a sur...
Article
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Background and Objectives: Cataract is a disease that is globally prevalent in today’s population and occurs mostly in the elderly. It is an opacity of the lens that worsens vision and can lead to blindness. One well-known risk factor of cataract is ultraviolet (UV) radiation. However, increasing exposure to modern artificial light sources like lig...
Preprint
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Nicht nur ultraviolette Strahlung wirkt antimikrobiell. Auch sichtbares violettes und blaues Licht kann Bakterien, Pilze und Viren reduzieren und aufgrund ihres Blauanteils gilt das auch für das Licht weißer LEDs. Diese Wirkung weißen LED-Lichts, die eher schwach ist, kann durch Kombination mit violettem Licht verstärkt werden, aber erfordert auch...
Article
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Purpose Inside the eye light can be reflected multiple times due to light-tissue interactions and the spherical geometry of the eye. Due to these optical properties, a defined retinal area is not only illuminated by direct light but also by indirect, reflected light from the inner side of the eyewall. During illumination for ophthalmic surgery, thi...
Article
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The COVID-19 pandemic is driving the search for new antiviral techniques. Bacteria and fungi are known to be inactivated not only by ultraviolet radiation but also by visible light. Several studies have recently appeared on this subject, in which viruses were mainly irradiated in media. However, it is an open question to what extent the applied med...
Article
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The spread of infections, as in the coronavirus pandemic, leads to the desire to perform disinfection measures even in the presence of humans. UVC radiation is known for its strong antimicrobial effect, but it is also harmful to humans. Visible light, on the other hand, does not affect humans and laboratory experiments have already demonstrated tha...
Article
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Bacteria belonging to the group of ESKAPE pathogens are responsible for the majority of nosocomial infections. Due to the increase of antibiotic resistance, alternative treatment strategies are of high clinical relevance. In this context visible light as disinfection technique represents an interesting option as microbial pathogens can be inactivat...
Article
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Objective Ultraviolet radiation is known for its antimicrobial properties but unfortunately, it could also harm humans. Currently, disinfection techniques against SARS-CoV-2 are being sought that can be applied on air and surfaces and which do not pose a relevant thread to humans. In this study, the bacteriophage phi6, which like SARS-CoV-2 is an e...
Preprint
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Im Juni 2020 kam es in einem Schlachthof in der Nähe von Gütersloh zu einer der größten Coronavirus-Masseninfektionen in Deutschland mit über 1000 Infizierten. Der Betrieb musste stillgelegt werden bis Maßnahmen zur Luftdesinfektion installiert waren. Die tatsächliche Wirkung von Luftdesinfektionssystemen für Betreibe und andere Einrichtungen ist s...
Preprint
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Mit Far-UVC wird der Spektralbereich 200 – 230 nm bezeichnet. Im Gegensatz zur 254 nm UVC-Emission von Quecksilberdampflampen soll Far-UVC-Strahlung Krankheitserreger inaktivieren ohne dabei Menschen zu schädigen. Diese Eigenschaften wären sehr geeignet, um die Coronapandemie einzudämmen. Trotz bisherigen und vielversprechenden Studien ist noch wei...
Article
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Background: Touchscreens are usually microbially contaminated and can therefore act as fomites inside and outside healthcare environments. Due to the increasing use of such touchscreens and the growing awareness of infection risks, approaches that allow safe and automatic disinfection are desired. Ultraviolet (UV) irradiation, with its known antimi...
Data
Attachment to: Hessling M, Haag R, Sicks B. Review of microbial touchscreen contamination for the determination of reasonable ultraviolet disinfection doses. GMS Hyg Infect Control. 2021;16:Doc30. DOI: 10.3205/dgkh000401
Article
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Lesions of aural, nasal and tracheal cartilage are frequently reconstructed by complex surgeries which are based on harvesting autologous cartilage from other locations such as the rib. Cartilage tissue engineering (CTE) is regarded as a promising alternative to attain vital cartilage. Nevertheless, CTE with nearly natural properties poses a signif...
Article
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Background: In current vitrectomy, the surgeon guides the vitrectome and a rigid fiber light guide with one hand each. It would be desirable to have a free hand for other surgical instruments and maneuvers. Methods: In the feasibility study presented here, a 20 gauge vitrectome is equipped with a miniature white LED that could eliminate the need fo...
Article
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The ongoing coronavirus pandemic spreads through airborne transmission and is therefore difficult to contain. However, coronaviruses are highly sensitive to UVC, so UVC air disinfection systems should be able to inactivate the virus. Unfortunately, so far there are only few possibilities to test the reduction of airborne viruses or other pathogens....
Article
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Artificial respiration is saving lives especially in the COVID-19 pandemic, but it also carries the risk to cause ventilator-associated pneumonia (VAP). VAP is one of the most common and severe nosocomial infections, often leading to death and adding a major economic burden to the healthcare system. To prevent a proliferation of microbial pathogens...
Article
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It has been proven that visible light with a wavelength of about 405 nm exhibits an antimicrobial effect on bacteria and fungi if the irradiation doses are high enough. Hence, the question arises as to whether this violet light would also be suitable to inactivate SARS-CoV-2 coronaviruses. Therefore, a high-intensity light source was developed and...
Article
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Background: Diaphanoscopy is an old but still useful technique in ophthalmic diagnostics. Its application suffers somewhat from the fact that the light is strongly attenuated and red-shifted in color when the eye wall is transilluminated. Material and methods: A color adjustable diaphanoscope prototype is developed based on a powerful red-green-...
Preprint
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Beatmungsassoziierte Lungenentzündungen gehören zu den häufigsten Krankenhausinfektionen und verursachen nicht nur hohe Kosten für das Gesundheitssystem, sondern enden auch oft tödlich für den betroffenen Patienten. Mit neuen Endotrachealtuben, die von blauen oder violette LEDs beleuchtet werden, soll die Zahl dieser Fälle reduziert werden.
Conference Paper
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The resolution of small commercial fiber-optic based ophthalmic endoscopes is severely limited by the size of the incision and the employed trocar. Therefore, this study presents a new endoscope that features a CMOS camera at the distal end of the endoscope and thus in the patient's eye. A suitably developed RGBW LED illumination system successfull...
Article
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Background and Objectives: Cataract is still the leading cause of blindness. Its development is well researched for UV radiation. Modern light sources like LEDs and displays tend to emit blue light. The effect of blue light on the retina is called blue light hazard and is studied extensively. However, its impact on the lens is not investigated so f...
Article
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The antimicrobial impact of visible violet and blue light has been known for more than a century but hardly been applied for purposeful pathogen reduction or prevention. The disinfecting properties of wide-spread warm-white and cool-white light emitting diodes (LEDs) are investigated by irradiation of staphylococci with different LEDs and varying...
Article
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Interest in visible light irradiation as a microbial inactivation method has widely increased due to multiple possible applications. Resistance development is considered unlikely, because of the multi-target mechanism, based on the induction of reactive oxygen species by wavelength specific photosensitizers. However, the affected targets are still...
Article
Full-text available
The globally observed rise in bacterial resistance against antibiotics has increased the need for alternatives to antibiotic treatments. The most prominent and important pathogen bacteria are the ESKAPE pathogens, which include among others Staphylococcus aureus, Klebsiella pneumoniae and Acinetobacter baumannii. These species cause ventilator-asso...
Article
Full-text available
Background: The ongoing coronavirus pandemic requires new disinfection approaches, especially for airborne viruses. The 254 nm emission of low-pressure vacuum lamps is known for its antimicrobial effect, but unfortunately, this radiation is also harmful to human cells. Some researchers published reports that short-wavelength ultraviolet light in th...
Article
Full-text available
Due to the globally observed increase in antibiotic resistance of bacterial pathogens and the simultaneous decline in new antibiotic developments, the need for alternative inactivation approaches is growing. This is especially true for the treatment of infections with the problematic ESKAPE pathogens, which include Enterococcus faecium, Staphylococ...
Article
Full-text available
For the visualisation of the intraocular space it is essential to illuminate the inside of the eye. One illumination approach is diaphanoscopy, in which the light of an extraocular source is transmitted through the tissue layers of the eyewall. The transmission properties of these tissues and their irradiation load depend on applied diaphanoscope c...
Article
Full-text available
To stop the coronavirus spread, new inactivation approaches are being sought that can also be applied in the presence of humans or even on humans. Here, we investigate the effect of visible violet light with a wavelength of 405 nm on the coronavirus surrogate phi6 in two aqueous solutions that are free of photosensitizers. A dose of 1300 J c/m2 of...
Article
Microorganisms can be photoinactivated with 405 and 450 nm irradiation, due to endogenous photosensitizers, which absorb light of these wavelengths and generate reactive oxygen species that destroy the cells from within. The photosensitizers assumed to be responsible are porphyrins in the spectral region around 405 nm and flavins at about 450 nm. T...
Article
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Multiple use contact lenses have to be disinfected overnight to reduce the risk of infections. However, several studies demonstrated that not only microorganisms are affected by the disinfectants, but also ocular epithelial cells, which come into contact via residuals at reinsertion of the lens. Visible light has been demonstrated to achieve an ina...
Article
Background: Ventilator-associated pneumonia (VAP) is one of the most common nosocomial infections in intensive care units, which not only leads to prolonged hospital stays and higher treatment costs but is also associated with high mortality. Objective: An approach to equip endotracheal tubes (ETT) with blue LEDs for photoinactivation of bacterial...
Data
Coronavirus inactivation data belonging to "Selection of parameters for thermal coronavirus inactivation – a data-based recommendation"
Article
Full-text available
Background: Healthcare workers and large parts of the population are currently using personal protective equipment, such as face masks, to avoid infections with the novel coronavirus SARS-CoV-2. This equipment must be sterilized as gently as possible before reuse. One possibility is thermal inactivation, but professional autoclaves with their high...
Article
Full-text available
Background: To slow the increasing global spread of the SARS-CoV-2 virus, appropriate disinfection techniques are required. Ultraviolet radiation (UV) has a well-known antiviral effect, but measurements on the radiation dose necessary to inactivate SARS-CoV-2 have not been published so far. Methods: Coronavirus inactivation experiments with ultrav...
Article
Purpose For transscleral application of optical radiation or light to the eye, it is important to know the transmission and absorbance of the tissue layers of the eyewall. The impact of photochemical energy to the retina located directly at the point of contact with the radiation emitter must be considered as well as the absorbance in choroid and r...
Article
This study presents a device for efficient, low-cost and eye-friendly overnight disinfection of contact lenses by visible violet light as an alternative to disinfection with biocide-containing solutions. Bacterial solutions with one Pseudomonas and one Staphylococcus strain each were irradiated for up to 8 h in commercial transparent contact lens c...
Article
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Despite the high number of legionella infections, there are currently no convincing preventive measures. Photoinactivation with visible light is a promising new approach and the photoinactivation sensitivity properties of planktonic Legionella rubrilucens to 450, 470, and 620 nm irradiation were thus investigated and compared to existing 405 nm ina...
Article
Inactivation properties of visible light are of increasing interest due to multiple possible fields of application concerning antibacterial treatment. For violet wavelengths, the generation of reactive oxygen species by porphyrins is accepted as underlying mechanism. However, there is still little knowledge about photosensitizers at blue wavelength...
Article
Touch screen displays are potential pathogen reservoirs and involved in the spread of hospital acquired infections. They emit visible light that is known for a weak but proven antimicrobial photoinactivation effect, so the question is whether displays have the potential to disinfect themselves. To test the antimicrobial capacity of touch screen dis...
Article
Full-text available
Introduction All applied illumination systems are validated according to a standard that measures in an experimental setup the direct radiation intensity on a surface in an aqueous solution, not involving an eyeball. Due to various factors, multiple intraocular light-tissue interactions could occur and lead to retinal irradiation intensities that a...
Article
Full-text available
Photoinactivation of bacteria with visible light has been reported in numerous studies. Radiation around 405 nm is absorbed by endogenous porphyrins and generates reactive oxygen species that destroy bacteria from within. Blue light in the spectral range of 450–470 nm also exhibits an antibacterial effect, but it is weaker than 405 nm radiation, an...
Article
Full-text available
The CAALA (Complex Augmentation of ALA) regimen was developed with the goal of redressing some of the weaknesses of 5-aminolevulinic acid (5-ALA) use in glioblastoma treatment as it now stands. 5-ALA is approved for use prior to glioblastoma surgery to better demarcate tumor from brain tissue. 5-ALA is also used in intraoperative photodynamic treat...
Article
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In the current discussion about the possible dangers and health consequences of blue or white LED light for various lighting purposes including displays, it is often ignored that LEDs are not the only light sources with a significant blue component. Fluorescent tubes or energy‐saving lamps have been used for decades, although they also have emissio...
Preprint
The CAALA (Complex Augmentation of ALA) regimen was developed with the goal of redressing some of the weaknesses of 5-aminolevulinic acid (5-ALA) use in glioblastoma treatment as it now stands. 5-ALA is approved for use prior to glioblastoma surgery to better demarcate tumor from brain tissue. 5-ALA is also used in intraoperative photodynamic treat...
Article
Full-text available
In a recently developed perfusion bioreactor, human nasal septum chondrocytes were seeded on decellularized porcine collagen scaffolds and cultivated for 6 weeks. Afterwards, the samples were stained with alcian blue and multiple histological microscope images were taken to assess the migration of the human cells and their redifferentiation. In thi...
Article
Background The increasing use of light-emitting diodes (LEDs) for lighting applications and displays is giving rise to public and professional concern that blue LED emissions could damage the retina or via the proven influence on the melatonin levels disrupt the human day-night rhythm. Objective The study aimed to measure the emission of LEDs and...
Article
Full-text available
Legionella infections caused by contaminated water are a widespread problem worldwide. Discharge lamps like mercury vapor lamps are widely known for the disinfection properties of their radiation, but they suffer technical disadvantages, like high voltages and toxic content, and are, therefore, not suitable for some infection control applications....
Article
Purpose: Surgical or diagnostic procedures are often accompanied by a short-term increase in intraocular pressure (IOP). A short-term increase in IOP can occur during refractive procedures, vitreoretinal surgery, transillumination, photocoagulation, or cryocoagulation. A porcine eye model was chosen (n = 89) to compile comparable study data and to...
Article
Full-text available
Purpose: Development of a new, fiber-free, single-use endo-illuminator for pars plana vitrectomy as a replacement for fiber-based systems with external light sources. The hand-guided intraocularly placed white micro light-emitting diode is evaluated for its illumination properties and potential photochemical and thermal hazards. Methods: A micro li...
Article
Die Zahl der jährlichen Legionelleninfektionen in Deutschland wird auf weit über 100 000 Fälle geschätzt. Eine mit dem WGKT-Innovationspreis 2017 ausgezeichnete Bachelorarbeit von Julian Schmid (Hochschule Ulm) wies auf neue Desinfektionsansätze hin.
Article
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Human chondrocytes were seeded on porcine collagen scaffolds and cultivated for up to six weeks in a cartilage bioreactor. To evaluate the influence of cultivation parameters on the proliferation and migration of the cells into the scaffolds, microscopic images from histological and immunohistochemical stainings were taken and digitalized. For eval...
Article
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Purpose: For diaphanoscopy or transscleral laser applications, the transmission of the sclera is an essential property. The study aimed to determine the pressure dependent transmission of human sclera from anterior to posterior. Methods: Pressure dependent transmission measurements were performed by a pressure inducing setup at the range of 60-2...
Article
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Knorpel in Ohren, Nase und Luftröhre bestimmt deren Form und biomechanische Eigenschaften. Kommt es aufgrund von Unfällen oder Erkrankungen zu einem Knorpelverlust, kann dies bei Ohr und Nase zu einer erheblichen funktionellen und psychischen Belastung des Patienten führen. Bei der Luftröhre sind sogar lebensbedrohliche Folgen möglich. Bisherige Be...
Article
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A list of the globally most important pathogens is generated based on the causes of death statistics published in the Global Burden Disease study 2015. Pathogens are assigned to the specified diseases as far as possible. Difficulties arises because the death provoking pathogens are often unidentified or arise in mixed infections with more than one...
Article
Existing light sources for intraocular illumination are often bulky and expensive and pose a risk for the patient, because light guides are inserted in the eye through incisions and if the tip of these light guides get too close to the retina, the retina can be damaged photochemically within minutes or even seconds. Therefore a new, safe and simple...
Patent
Vorrichtung zur einfachen und effizienten Fluoreszenzanregung in Realtime-Thermocyclern mit einer oder mehreren LEDs